From 2D slices to 3D volumes: Image based reconstruction and morphological characterization of hippocampal cells on charged and uncharged surfaces using FIB/SEM serial sectioning

Ultramicroscopy

Volumn 111, Issue 4, 2011, Pages 259-266

  Schmidt, Franziska ^a   Kühbacher, Markus ^b   Gross, Ulrich ^a,c   Kyriakopoulos, Antonius ^b   Schubert, Helmut ^a   Zehbe, Rolf ^a  

^a TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^b HAHN MEITNER INSTITUT   (Germany)

^c CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

3D morphology; Cell death; Cell spreading; EDX; Electron microscopy; Focused ion beam; Neuronal cells

Indexed keywords

2-D SLICE; 3D IMAGING; 3D MORPHOLOGY; ABSORBING STRUCTURE; ARTIFICIAL OBJECTS; CELL SPREADING; CHROMATIN CONDENSATION; DUAL BEAM; EDX; EXPERIMENTAL SETUP; EXTREME DEVIATIONS; FIB/SEM; GOLD MICROELECTRODES; HIPPOCAMPAL CELLS; IMAGE-BASED; LIFE-SCIENCES; LIMITING FACTORS; MORPHOLOGICAL CHARACTERIZATION; NEURONAL CELL; PENETRATION DEPTH; POLYMER SUBSTRATE; SERIAL SECTIONING; STATE OF THE ART; SUBCELLULAR RESOLUTION; TOMOGRAPHIC; X RAY MICROSCOPY;

BEAM PLASMA INTERACTIONS; CELL CULTURE; CELL DEATH; CHEMICAL ANALYSIS; COMPUTERIZED TOMOGRAPHY; ELECTRIC POTENTIAL; ELECTRON BEAMS; ELECTRONS; FOCUSED ION BEAMS; GOLD COATINGS; ION BOMBARDMENT; IONS; MICROELECTRODES; MORPHOLOGY; SCANNING ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY; TWO DIMENSIONAL; X RAYS;

THREE DIMENSIONAL;

ANIMAL CELL; ARTICLE; BRAIN CELL; CELL STRAIN; CELL STRUCTURE; CHROMATIN CONDENSATION; CONTROLLED STUDY; ELECTRIC POTENTIAL; ELECTRON BEAM; ENERGY DISPERSIVE X RAY SPECTROSCOPY; FOCUSED ION BEAM; HIPPOCAMPUS; IMAGE RECONSTRUCTION; MICRO-COMPUTED TOMOGRAPHY; MICROELECTRODE; MOUSE; NERVE CELL NECROSIS; NEUROIMAGING; NONHUMAN; OPTICAL RESOLUTION; ROENTGEN SPECTROSCOPY; SCANNING ELECTRON MICROSCOPY; SURFACE PROPERTY; THREE DIMENSIONAL IMAGING; X RAY MICROANALYSIS;

ANIMALS; CELL LINE; ELECTRODES; GOLD; HIPPOCAMPUS; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; IMAGE PROCESSING, COMPUTER-ASSISTED; IMAGING, THREE-DIMENSIONAL; MICE; MICROSCOPY, ELECTRON, SCANNING; POLYMERS;

EID: 78651349103     PISSN: 03043991     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ultramic.2010.12.017     Document Type: Article

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