Site-specific 3D imaging of cells and tissues with a dual beam microscope

Journal of Structural Biology

Volumn 155, Issue 1, 2006, Pages 63-73

  Heymann, Jurgen A W ^a   Hayles, Mike ^b   Gestmann, Ingo ^b   Giannuzzi, Lucille A ^b   Lich, Ben ^b   Subramaniam, Sriram ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b Thermo Fisher Scientific   (United States)

Author keywords

3D cellular imaging; Cryo electron microscopy; Dual beam imaging; Focused ion beam; Tumor tissue; Yeast

Indexed keywords

ARTICLE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; ELECTRON MICROSCOPY; HUMAN; HUMAN CELL; HUMAN TISSUE; LYMPHOID TISSUE; NONHUMAN; PRIORITY JOURNAL; ROOM TEMPERATURE; SCANNING ELECTRON MICROSCOPY; SURFACE PROPERTY; THREE DIMENSIONAL IMAGING;

ANIMALS; CELL SHAPE; CELLS, CULTURED; CRYOELECTRON MICROSCOPY; DIAGNOSTIC IMAGING; FREEZE DRYING; FROZEN SECTIONS; LYMPHOID TISSUE; MICROSCOPY, ELECTRON, TRANSMISSION; MICROTOMY; MODELS, BIOLOGICAL; PLASTIC EMBEDDING; SENSITIVITY AND SPECIFICITY; TEMPERATURE; YEAST, DRIED; YEASTS;

EID: 33744979750     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2006.03.006     Document Type: Article

References (26)

1. Ballerini M., Milani M., Batani M., and Squadrini F. Focused ion beam techniques for the analysis of biological samples: a revolution in ultramicroscopy?. Proc. SPIE 4261 (2001) 92-104
2. Barnard D.P., Turner J.N., Frank J., and McEwen B.F. A 360 degrees single-axis tilt stage for the high-voltage electron microscope. J. Microsc. 167 Pt 1 (1992) 39-48
3. Bauer C., Herzog V., and Bauer M.F. Improved technique for electron microscope visualization of yeast membrane structure. Microsc. Microanal. 7 (2001) 530-534
4. Denk W., and Horstmann H. Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure. PLoS Biol. 2 (2004) e329
5. Drobne D., Milani M., Zrimec A., Zrimec M.B., Tatti F., and Draslar K. Focused ion beam/scanning electron microscopy studies of Porcellio scaber (Isopoda, Crustacea) digestive gland epithelium cells. Scanning 27 (2005) 30-34
6. Dubochet J., Adrian M., Chang J.J., Homo J.C., Lepault J., McDowall A.W., and Schultz P. Cryo-electron microscopy of vitrified specimens. Q. Rev. Biophys. 21 (1988) 129-228
7. Forster F., Medalia O., Zauberman N., Baumeister W., and Fass D. Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography. Proc. Natl. Acad. Sci. USA 102 (2005) 4729-4734
8. Gestmann I., Hayles M., Shi D., Kumar G., Giannuzzi L.A., Lich B., and Subramaniam S. Site-specific 3D imaging of cells and tissues using dual beam technology. Microsc. Microanal. 10 (2004) 1124-1125
9. Giannuzzi, L.A., Kempshall, B.W., Anderson, S.D., Prenitzer, B.I., Moore, T.M., 2002. FIB Lift-Out for Defect Analysis. Analysis Techniques of Submicron Defects, 2002 Supplement to the EDFAS Analysis Desktop Reference, 29-35.
10. In: Giannuzzi L.A., and Stevie F.A. (Eds). Introduction to Focused Ion Beams (2005), Springer, New York
11. Heymann J., Hayles M., Gestmann I., Shi D., Lich B., and Subramaniam S. Site-specific, automated 3D imaging of cells and tissues using a dual beam microscope. Microsc. Microanal. 11 Suppl 2 (2005) 858
12. Kremer J.R., Mastronarde D.N., and McIntosh J.R. Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116 (1996) 71-76
13. Larabell C.A., and Le Gros M.A. X-ray tomography generates 3-D reconstructions of the yeast, Saccharomyces cerevisiae, at 60-nm resolution. Mol. Biol. Cell 15 (2004) 957-962
14. Lee S., and Subramaniam S. Use of cylindrically symmetric specimen chambers for full tilt electron tomography. Biophys. J. 86 (2004) supplement, 80a
15. Marsh B.J., Volkmann N., McIntosh J.R., and Howell K.E. Direct continuities between cisternae at different levels of the Golgi complex in glucose-stimulated mouse islet beta cells. Proc. Natl. Acad. Sci. USA 101 (2004) 5565-5570
16. McIntosh R., Nicastro D., and Mastronarde D. New views of cells in 3D: an introduction to electron tomography. Trends Cell Biol. 15 (2005) 43-51
17. Mulders H. The use of a SEM/FIB dual beam applied to biological samples. G.I.T. Imaging Microsc. 2 (2003) 8-10
18. Rubanov S., and Munroe. Damage in III-V compounds during focused ion beam milling. Microsc. Microanal. 11 (2005) 446-455
19. Subramaniam S. Bridging the imaging gap: visualizing subcellular architecture with electron tomography. Curr. Opin. Microbiol. 8 (2005) 316-322
20. Subramaniam S., and Milne J.L. Three-dimensional electron microscopy at molecular resolution. Annu. Rev. Biophys. Biomol. Struct. 33 (2004) 141-155
21. Sugiyama M., and Sigesato G. A review of focused ion beam technology and its applications in transmission electron microscopy. J. Electron Microsc. (Tokyo) 53 (2004) 527-536
22. Tsai P.S., Friedman B., Ifarraguerri A.I., Thompson B.D., Lev-Ram V., Schaffer C.B., Xiong Q., Tsien R.Y., Squier J.A., and Kleinfeld D. All-optical histology using ultrashort laser pulses. Neuron 39 (2003) 27-41
23. Wallis J.W., Chrebet G., Brodsky G., Rolfe M., and Rothstein R. A hyper-recombination mutation in S. cerevisiae identifies a novel eukaryotic topoisomerase. Cell 58 (1989) 409-419
24. Weiss D., Schneider G., Niemann B., Guttmann P., Rudolph D., and Schmahl G. Computed tomography of cryogenic biological specimens based on X-ray microscopic images. Ultramicroscopy 84 (2000) 185-197
25. Young R.J., Dingle T., Robinson K., and Pugh P.J.A. An application of scanned focused ion beam milling to studies on the internal morphology of small arthropods. J. Microsc. 172 (1993) 81-88
26. Zhang P., Land W., Lee S., Juliani J., Lefman J., Smith S.R., Germain D., Kessel M., Leapman R., Rouault T.A., and Subramaniam S. Electron tomography of degenerating neurons in mice with abnormal regulation of iron metabolism. J. Struct. Biol. 150 (2005) 144-153