Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy

Science

Volumn 320, Issue 5881, 2008, Pages 1332-1336

  Schermelleh, Lothar ^a   Carlton, Peter M ^b   Haase, Sebastian ^b,d   Shao, Lin ^b   Winoto, Lukman ^b   Kner, Peter ^b   Burke, Brian ^c   Cardoso, M Cristina ^d   Agard, David A ^b   Gustafsson, Mats G L ^e   Leonhardt, Heinrich ^a   Sedat, John W ^b  

^a UNIVERSITY OF MUNICH   (Germany)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF FLORIDA   (United States)

^d MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^e UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LAMIN; 4',6 DIAMIDINO 2 PHENYLINDOLE; FLUORESCENT DYE; INDOLE DERIVATIVE;

ELECTRON MICROSCOPY; IMAGING METHOD; SPATIAL RESOLUTION; THREE-DIMENSIONAL MODELING; VISUALIZATION;

ARTICLE; CELL NUCLEUS; CELL NUCLEUS MEMBRANE; CHROMATIN; COLOR; DIFFRACTION; FLUORESCENCE MICROSCOPY; HETEROCHROMATIN; IMAGE ANALYSIS; IMAGE QUALITY; IMAGE RECONSTRUCTION; IMAGING SYSTEM; NUCLEAR PORE COMPLEX; PRIORITY JOURNAL; PROPHASE; PROTEIN LOCALIZATION; SUBDIFFRACTION MULTICOLOR IMAGING; THREE DIMENSIONAL STRUCTURED ILLUMINATION MICROSCOPY; ANIMAL; CELL LINE; CONFOCAL MICROSCOPY; INSTRUMENTATION; INTERPHASE; METHODOLOGY; MOUSE; MYOBLAST; NUCLEAR LAMINA; NUCLEAR PORE; OPTICS; THREE DIMENSIONAL IMAGING; ULTRASTRUCTURE;

MAMMALIA;

ANIMALS; CELL LINE; CELL NUCLEUS; CHROMATIN; FLUORESCENT DYES; HETEROCHROMATIN; IMAGING, THREE-DIMENSIONAL; INDOLES; INTERPHASE; LAMINS; MICE; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MYOBLASTS; NUCLEAR ENVELOPE; NUCLEAR LAMINA; NUCLEAR PORE; OPTICS;

EID: 45549091077     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1156947     Document Type: Article

References (30)

1. M. Born, E. Wolf, Eds., Principle of Optics (Cambridge Univ. Press, Cambridge, 1998).
2. J. B. Pawley, Ed., Handbook of Biological Confocal Microscopy (Springer, New York, ed. 3, 2006).
3. E. Abbe, Arch. Mikrosk. Anat. 9, 413 (1873).
4. M. G. Gustafsson et al., Biophys. J., in press; published online 7 March 2008 (10.1529/biophysj.107.120345).
5. M. G. Gustafsson, J. Microsc. 198, 82 (2000).
6. R. Heintzmann, G. Ficz, Brief. Funct. Genomics Proteomics 5, 289 (2006).
7. S. W. Hell, Nat. Biotechnol. 21, 1347 (2003).
8. S. W. Hell, Science 316, 1153 (2007).
9. R. J. Kittel et al., Science 312, 1051 (2006); published online 13 April 2006 (10.1126/science.1126308).
10. K. I. Willig, S. O. Rizzoli, V. Westphal, R. Jahn, S. W. Hell, Nature 440, 935 (2006).
11. Material and methods are available on Science Online.
12. R. Reichelt et al., J. Cell Biol. 110, 883 (1990).
13. B. Burke, C. L. Stewart, Nat. Rev. Mol. Cell Biol. 3, 575 (2002).
14. M. R. Paddy, A. S. Belmont, H. Saumweber, D. A. Agard, J. W. Sedat, Cell 62, 89 (1990).
15. M. Beck et al., Science 306, 1387 (2004); published online 28 October 2004 (10.1126/science.1104808).
16. M. Beck, V. Lucic, F. Forster, W. Baumeister, O. Medalia, Nature 449, 611 (2007).
17. D. Stoffler et al., J. Mol. Biol. 328, 119 (2003).
18. D. A. Agard, Y. Hiraoka, P. Shaw, J. W. Sedat, Methods Cell Biol. 30, 353 (1989).
19. H. Albiez et al., Chromosome Res. 14, 707 (2006).
20. L. Gerace, A. Blum, G. Blobel, J. Cell Biol. 79, 546 (1978).
21. B. Fahrenkrog et al., J. Struct. Biol. 140, 254 (2002).
22. R. Tonini, F. Grohovaz, C. A. Laporta, M. Mazzanti, FASEB J. 13, 1395 (1999).
23. M. Winey, D. Yarar, T. H. Giddings Jr., D. N. Mastronarde, Mol. Biol. Cell 8, 2119 (1997).
24. M. Fricker, M. Hollinshead, N. White, D. Vaux, J. Cell Biol. 136, 531 (1997).
25. M. Bates, B. Huang, G. T. Dempsey, X. Zhuang, Science 317, 1749 (2007); published online 15 August 2007 (10.1126/science.1146598).
26. E. Betzig et al., Science 313, 1642 (2006); published online 9 August 2006 (10.1126/science.1127344).
27. G. Donnert et al., Proc. Natl. Acad. Sci. U.S.A. 103, 11440 (2006).
28. B. Huang, W. Wang, M. Bates, X. Zhuang, Science 319, 810 (2008); published online 2 January 2008 (10.1126/science.1153529).
29. M. J. Rust, M. Bates, X. Zhuang, Nat. Methods 3, 793 (2006).
30. This work was supported by grants from the Bavaria California Technology Center, the Center for NanoScience, the Nanosystems Initiative Munich, and the Deutsche Forschungsgemeinschaft to L. Schermelleh, M.C.C., and H.L.; by NIH grant GM-2501-25 to J.W.S.; by the David and Lucile Packard Foundation; and by NSF through the Center for Biophotonics, an NSF Science and Technology Center managed by the University of California, Davis, under cooperative agreement no. PHY 0120999. P.M.C. is partially supported by the Keck Laboratory for Advanced Microscopy at the University of California, San Francisco. We thank A. Čopïč, K. Weis, and F. Spada for comments on the manuscript and helpful discussions. P.M.C., L. Shao, L.W., and P.K. have performed limited paid consulting for Applied Precision, which is planning a commercial microscope system using three-dimensional structured illumination. The University of California holds patents for structured illumination microscopy.