Highly site-selective stability increases by glycosylation of dihydrofolate reductase

FEBS Journal

Volumn 277, Issue 9, 2010, Pages 2171-2179

  Tey, Lai Hock ^a   Loveridge, E Joel ^a   Swanwick, Richard S ^a   Flitsch, Sabine L ^b   Allemann, Rudolf K ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Enzyme; Glycosylation; Kinetics; Mutagenesis; Stability

Indexed keywords

CYSTEINE; DIHYDROFOLATE REDUCTASE; GLUCOSE; IODOACETAMIDE; LACTOSE; MALTOTRIOSE; N ACETYLGLUCOSAMINE; SUGAR;

ALKYLATION; ARTICLE; CATALYST; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME DENATURATION; ENZYME GLYCOSYLATION; ENZYME STABILITY; ESCHERICHIA COLI; LIGAND BINDING; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; TEMPERATURE STRESS; THERMOSTABILITY;

BIOCATALYSIS; CIRCULAR DICHROISM; ENZYME STABILITY; ESCHERICHIA COLI; GLYCOSYLATION; KINETICS; LIGANDS; MODELS, MOLECULAR; MUTATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; TETRAHYDROFOLATE DEHYDROGENASE; UNFOLDED PROTEIN RESPONSE;

ESCHERICHIA COLI; EUKARYOTA;

EID: 77951209387     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2010.07634.x     Document Type: Article

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