Selective in vitro glycosylation of recombinant proteins: Semi-synthesis of novel homogeneous glycoforms of human erythropoietin

Chemistry and Biology

Volumn 8, Issue 2, 2001, Pages 133-145

  Macmillan, Derek ^a   Bill, Roslyn M ^a   Sage, Karen A ^a   Fern, Dominic ^a   Flitsch, Sabine L ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Erythropoietin; Human; In vitro glycosylation; Recombinant protein; Semi synthetic glycoprotein

Indexed keywords

AFFINITY CHROMATOGRAPHY; ASPARAGINE; CHEMICAL REACTION; CYSTEINE; ERYTHROPOIETIN; ESCHERICHIA COLI; GLYCOSYL BETA N IODOACETAMIDE; MUTANT PROTEIN; PROTEIN GLYCOSYLATION; RECOMBINANT PROTEIN; WILD RELATIVE;

ESCHERICHIA COLI;

EID: 0035057706     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(00)90065-6     Document Type: Article

References (49)

1. Erythropoietin
2. Cloning and expression of the human erythropoietin gene
3. Efficiency of signalling through cytokine receptors depends critically on receptor orientation
4. Cytokine therapy: Lessons learned and future challenges
5. Microheterogeneity of erythropoietin carbohydrate structure
6. Lectin-binding assays for the isoforms of human erythropoietin: Comparison of urinary and four recombinant erythropoietins
7. Carbohydrate structure of erythropoietin expressed in chinese hamster ovary cells by a human erythropoietin cDNA
8. Effects of site directed removal of N-glycosylation sites in human erythropoietin on it's production and biological properties
9. The importance of N- and O-linked oligosaccharides for the biosynthesis and in vitro and in vivo biologic activities of erythropoietin
10. Isolation and characterisation of conformation sensitive antierythropoietin monoclonal antibodies: Effect of disulfide bonds and carbohydrate on recombinant human erythropoietin structure
11. Role of antennary structure of N-linked sugar chains in renal handling of recombinant human erythropoietin
12. N- and O-glycosylation muteins of recombinant human erythropoietin secreted from BHK-21 cells
13. Role of sugar chains in the expression of the biological activity of human erythropoietin
14. Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in chinese hamster ovary cells
15. The effect of carbohydrate on the structure and stability of erythropoietin
16. Erythropoietin structure-function relationships, mutant proteins that test a model of tertiary structure
17. Lectins as chaperones in protein folding
18. Synthesis of native proteins by chemical ligation
19. Adding splice to protein engineering
20. A new ligation method for N-terminal tryptophan-containing peptides using the Pictet-Spengler reaction
21. Putting protein splicing to work
22. Transfer active ester condensation: A novel technique for peptide segment coupling
23. Fmoc-based synthesis of peptide-α thioesters: Application to the total synthesis of a glycoprotein by native chemical ligation
24. Intein-mediated synthesis of peptides containing carbohydrates and other molecular probes
25. A highly convergent total synthetic route to glycopeptides carrying a high mannose core pentasaccharide domain N-linked to a natural peptide motif
26. A modular system for the synthesis of complex N-glycans
27. A practical and efficient synthesis of complex-type biantennary heptasaccharide-asparagine conjugate, a key building block for the synthesis of complex N-linked glycopeptides
28. Novel sequential solid-phase synthesis of N-linked glycopeptides from natural sources
29. A strategy for the chemoselective synthesis of O-linked glycopeptides with native sugar-peptide linkages
30. Carbohydrate mimetics: A new strategy for tackling the problem of carbohydrate-mediated biological recognition
31. New directions in the synthesis of glycopeptide mimetics
32. Recent developments in glycoconjugates
33. Effect of N-linked glycosylation on glycopeptide and glycoprotein structure
34. From the laboratory to the clinic: A retrospective on fully synthetic carbohydrate-based anticancer vaccines
35. Synthesis of structurally defined antigen-immunostimulant combination for use in cancer vaccines
36. A novel method for the specific glycosylation of proteins
37. Expression and mutagenesis of recombinant human and murine erythropoietins in Escherichia coli
38. Synthetic glycosylation of proteins using N-(beta-saccharide) iodoacetamides - Applications in site specific glycosylation and solid phase enzymatic oligosaccharide synthesis
39. A unique and highly facile method for synthesising disulfide linked neoglycoconjugates: A new approach for remodelling of peptides and proteins
40. Controlled site selective glycosylation of proteins by combined site directed mutagenesis and chemical modification approach
41. The identity of the base following the stop codon determines the efficiency of in vivo translational termination in Escherichia coli
42. UGA read-through artifacts - When popular gene expression systems need a pATCH
43. Advances in refolding of proteins produced in E. coli
44. Specific chemical cleavage in high yield at the amino peptide bonds of cysteine and cystine residues
45. NMR structure of human erythropoietin and a comparison with its receptor bound conformation
46. Biological roles of oligosaccharides: All of the theories are correct