Crystal structure of a "nonfoldable" insulin. Impaired folding efficiency despite native activity

Journal of Biological Chemistry

Volumn 284, Issue 50, 2009, Pages 35259-35272

  Liu, Ming ^a   Wan, Zhu Ii ^b   Chu, Ying Chi ^c   Aladdin, Hassan ^d   Klaproth, Birgit ^d   Choquette, Meredith ^e   Hua, Qing Xin ^b   Mackin, Robert B ^e   Rao, J Sunil ^b   De Meyts, Pierre ^d   Katsoyannis, Panayotis G ^c   Arvan, Peter ^a   Weiss, Michael A ^b  

^a UNIVERSITY OF MICHIGAN HEALTH SYSTEM   (United States)

^b CASE WESTERN RESERVE UNIVERSITY   (United States)

^c NEW YORK UNIVERSITY   (United States)

^d HAGEDORN RESEARCH INSTITUTE   (Denmark)

^e CREIGHTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL DYSFUNCTION; CONSERVED RESIDUES; DIABETES MELLITUS; ENDOPLASMIC RETICULUM; IN-VITRO; INSULIN ANALOGUES; INSULIN CHAIN; MAMMALIAN CELL CULTURE; MISFOLDING; NATIVE STATE; NON-LOCAL INTERACTIONS; PROINSULIN; PROTEIN EVOLUTION; REFOLDING; STRUCTURAL EFFECT; WILD-TYPE PROTEINS;

ANIMAL CELL CULTURE; CELL CULTURE; CRYSTAL STRUCTURE; MAMMALS; PROTEIN FOLDING;

INSULIN;

ALANINE; CYSTEINE; DISULFIDE; INSULIN; LEUCINE; PROINSULIN; VALINE;

ALPHA HELIX; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BIOLOGICAL ACTIVITY; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENDOPLASMIC RETICULUM; HUMAN; HUMAN CELL; IN VITRO STUDY; MAMMAL CELL; MOLECULAR EVOLUTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FOLDING; PROTEIN MISFOLDING; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN STRUCTURE; THERMODYNAMICS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; CELL LINE; DISULFIDES; HUMANS; INSULIN; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN FOLDING; PROTEIN PRECURSORS; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; THERMODYNAMICS; VALINE;

MAMMALIA;

EID: 71749116066     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.046888     Document Type: Article

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