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Volumn 73, Issue 1, 2001, Pages 74-79

Human insulin from a precursor overexpressed in the methylotrophic yeast Pichia pastoris and a simple procedure for purifying the expression product

(9) Wang, Yan a Liang, Zhen He a Zhang, You Shang a Yao, Shi Yin a Xu, Ying Gao a Tang, Yue Hua a Zhu, Shang Quan a Cui, Da Fu a Feng, You Min a

a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY (China)

Author keywords

Dot blotting method; Insulin precursor; Methylotrophic yeast

Indexed keywords

TRYPTIC TRANSPEPTIDATION;

CHROMOSOMES; FERMENTATION; GENES; METHANOL; PROTEINS; YEAST;

INSULIN;

PREPROINSULIN;

ARTICLE; CRYSTALLIZATION; CULTURE MEDIUM; DOT HYBRIDIZATION; ENZYME MECHANISM; FERMENTATION; NONHUMAN; PICHIA PASTORIS; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RECEPTOR BINDING; SIGNAL TRANSDUCTION; YEAST;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRYSTALLIZATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FERMENTATION; HUMANS; INSULIN; PICHIA; PLASMIDS; PROTEIN PRECURSORS; RECEPTOR, INSULIN; RECOMBINANT PROTEINS; TRANSFORMATION, GENETIC; ZINC;

PICHIA PASTORIS;

EID: 0035810269 PISSN: 00063592 EISSN: None Source Type: Journal
DOI: 10.1002/1097-0290(20010405)73:1<74::AID-BIT1038>3.0.CO;2-V Document Type: Article

Times cited : (67)

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