Intracellular delivery of a cell-penetrating SOCS1 that targets IFN-γ signaling

Science Signaling

Volumn 2, Issue 80, 2009, Pages

  DiGiandomenico, Antonio ^a   Wylezinski, Lukasz S ^a   Hawiger, Jacek ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOKINE; CYTOKINE; GAMMA INTERFERON; GAMMA INTERFERON INDUCIBLE PROTEIN 10; GRANULOCYTE COLONY STIMULATING FACTOR; INTERLEUKIN 6; RANTES; STAT1 PROTEIN; SUPPRESSOR OF CYTOKINE SIGNALING 1; SOCS1 PROTEIN, HUMAN; SOCS1 PROTEIN, MOUSE; STAT1 PROTEIN, HUMAN; STAT1 PROTEIN, MOUSE; SUPPRESSOR OF CYTOKINE SIGNALING;

ANIMAL CELL; ANTIINFLAMMATORY ACTIVITY; ARTICLE; ATTENUATION; CYTOKINE PRODUCTION; DRUG DELIVERY SYSTEM; DRUG TARGETING; ESCHERICHIA COLI; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; WESTERN BLOTTING; ANIMAL; CELL LINE; INFLAMMATION; METABOLISM; MOUSE;

ANIMALS; CELL LINE; HUMANS; INFLAMMATION; INTERFERON-GAMMA; MICE; SIGNAL TRANSDUCTION; STAT1 TRANSCRIPTION FACTOR; SUPPRESSOR OF CYTOKINE SIGNALING PROTEINS;

EID: 70349562245     PISSN: 19450877     EISSN: 19379145     Source Type: Journal    
DOI: 10.1126/scisignal.1162191     Document Type: Article

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54. We thank M. Shong (Chungnam National University, Korea) for providing mouse Socs1 cDNA with permission from D. J. Hilton (Royal Melbourne Hospital, Australia). We thank Y. Durocher (National Research Council of Canada) for providing our laboratory with the HEK 293-6E mammalian protein expression system. We also thank S. Richards for assistance in the preparation of this manuscript. U.S. NIH National Heart, Lung and Blood Institute (NHLBI) grants RO1 HL069452 and PO1 HL68744 supported this work. A.D. was supported by a NIH/NHLBI Immunobiology of Blood and Vascular Systems Training Grant (T32 HL069765) and a Ruth L. Kirschstein National Research Service Awards Individual Fellowship (F32 HL087531). The use of core facilities in this study was supported by the US NIH 2P30 CA 68485 to the Vanderbilt Ingram Cancer Center and by 5P30DK058404 to the Vanderbilt Digestive Disease Research Center.