What transmission electron microscopes can visualize now and in the future

Journal of Structural Biology

Volumn 163, Issue 3, 2008, Pages 235-245

  Müller, Shirley A ^a   Aebi, Ueli ^a   Engel, Andreas ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

Author keywords

Automated TEM; Electron crystallography; Electron tomography; Low dose EM; Single cell proteomics; STEM mass determination; Transmission electron microscopy microscope (TEM)

Indexed keywords

ALPHA ACTIN; AQUAPORIN; AQUAPORIN O; BACTERIAL TOXIN; CYTOTOXIN; MEMBRANE PROTEIN; PHALLOIDIN; PULLULANASE; UNCLASSIFIED DRUG;

ACTIN FILAMENT; ARTICLE; FIELD EMISSION SCANNING ELECTRON MICROSCOPY; KLEBSIELLA OXYTOCA; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; SCANNING TRANSMISSION ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY; X RAY CRYSTALLOGRAPHY; YERSINIA;

COMPUTING METHODOLOGIES; FORECASTING; FREEZING; HISTORY, 20TH CENTURY; HISTORY, 21ST CENTURY; MACROMOLECULAR SUBSTANCES; MICROSCOPY, ELECTRON, TRANSMISSION; PROTEINS; SPECIMEN HANDLING;

BACTERIA (MICROORGANISMS); MAMMALIA; YERSINIA;

EID: 51349168500     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2008.05.008     Document Type: Article

References (113)

1. Abu-Arish A., Frenkiel-Krispin D., Fricke T., Tzfira T., Citovsky V., Wolf Sharon G., and Elbaum M. Three-dimensional reconstruction of Agrobacterium Vire2 protein with single-stranded DNA. J. Biol. Chem. 279 (2004) 25359-25363
2. Adrian M., Dubochet J., Lepault J., and McDowall A.W. Cryo-electron microscopy of viruses. Nature 308 (1984) 32-36
3. Adrian M., Dubochet J., Fuller S.D., and Harris J.R. Cryo-negative staining. Micron 29 (1998) 145-160
4. Al-Amoudi A., Chang J.-J., Leforestier A., McDowall A., Salamin L.M., Norlen L.P.O., Richter K., Blanc N.S., Studer D., and Dubochet J. Cryo-electron microscopy of vitreous sections. EMBO J. 23 (2004) 3583-3588
5. Al-Amoudi A., Norlen Lars P.O., and Dubochet J. Cryo-electron microscopy of vitreous sections of native biological cells and tissues. J. Struct. Biol. 148 (2004) 131-135
6. Al-Amoudi A., Studer D., and Dubochet J. Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy. J. Struct. Biol. 150 (2005) 109-121
7. Baer H., Muecke N., Ringler P., Müller S.A., Kreplak L., Katus H.A., Aebi U., and Herrmann H. Impact of disease mutations on the desmin filament assembly process. J. Mol. Biol. 360 (2006) 1031-1042
8. Balcome, G.E., Splittstoesser, K.A., 2005. SEM sample holder apparatus for implementing enhanced examination of multiple samples, 7 pp. (International Business Machines Corporation, USA). Application: US.
9. Ballweber E., Hannappel E., Huff T., Stephan H., Haener M., Taschner N., Stoffler D., Aebi U., and Mannherz Hans G. Polymerisation of chemically cross-linked actin:thymosin beta(4) complex to filamentous actin: alteration in helical parameters and visualisation of thymosin beta(4) binding on F-actin. J. Mol. Biol. 315 (2002) 613-625
10. Baumeister W. A voyage to the inner space of cells. Protein Sci. 14 (2005) 257-269
11. Beck M., Lucic V., Foerster F., Baumeister W., and Medalia O. Snapshots of nuclear pore complexes in action captured by cryo-electron tomography. Nature 449 (2007) 611-615
12. Beck M., Forster F., Ecke M., Plitzko J.M., Melchior F., Gerisch G., Baumeister W., and Medalia O. Nuclear pore complex structure and dynamics revealed by cryoelectron tomography. Science 306 (2004) 1387-1390
13. Bouchet-marquis C., Zuber B., Glynn A.-M., Eltsov M., Grabenbauer M., Goldie K.N., Thomas D., Frangakis A.S., Dubochet J., and Chretien D. Visualization of cell microtubules in their native state. Biol. Cell 99 (2007) 45-53
14. Bremer A., Henn C., Engel A., Baumeister W., and Aebi U. Has negative staining still a place in biomacromolecular electron microscopy?. Ultramicroscopy 46 (1992) 85-111
15. Bremer A., Henn C., Goldie K.N., Engel A., Smith P.R., and Aebi U. Towards atomic interpretation of F-actin filament three-dimensional reconstructions. J. Mol. Biol. 242 (1994) 683-700
16. Broz P., Mueller C.A., Müller S.A., Philippsen A., Sorg I., Engel A., and Cornelis G.R. Function and molecular architecture of the Yersinia injectisome tip complex. Mol. Microbiol. 65 (2007) 1311-1320
17. Burghout P., Van Boxtel R., Van Gelder P., Ringler P., Müller S.A., Tommassen J., and Koster M. Structure and electrophysiological properties of the YscC secretin from the type III secretion system of Yersinia Enterocolitica. J. Bacteriol. 186 (2004) 4645-4654
18. Buser C., Walther P., Mertens T., and Michel D. Cytomegalovirus primary envelopment occurs at large infoldings of the inner nuclear membrane. J. Virol. 81 (2007) 3042-3048
19. Cornelis G.R. The type III secretion injectisome. Nat. Rev. Microbiol. 4 (2006) 811-825
20. Cyrklaff M., Linaroudis A., Boicu M., Chlanda P., Baumeister W., Griffiths G., and Krijnse-Locker J. Whole cell cryo-electron tomography reveals distinct disassembly intermediates of Vaccinia virus. PLoS One 2 5 (2007)
21. Denk W., and Horstmann H. Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure. PLoS Biol. 2 (2004) 1900-1909
22. Di Carlo D., Wu Liz Y., and Lee Luke P. Dynamic single cell culture array. Lab Chip 6 (2006) 1445-1449
23. Di Carlo D., Ionescu-Zanetti C., Zhang Y., Hung P., and Lee Luke P. On-chip cell lysis by local hydroxide generation. Lab Chip 5 (2005) 171-178
24. de Groot B.L., and Grubmüller H. The dynamics and energetics of water permeation and proton exclusion in aquaporins. Curr. Opin. Struct. Biol. 15 (2005) 176-183
25. Dowell R.D., Jokerst R.M., Day A., Eddy S.R., and Stein L. The distributed annotation system. BMC Bioinform. 2 (2001) e420 ([online computer file)
26. Dube P., Herzog F., Gieffers C., Sander B., Riedel D., Müller S.A., Engel A., Peters J.-M., and Stark H. Localization of the coactivator Cdh1 and the cullin subunit Apc2 in a cryo-electron microscopy model of vertebrate APC/C. Mol. Cell 20 (2005) 867-879
27. Dubochet J., Adrian M., Chang J.J., Homo J.C., Lepault J., McDowall A.W., and Schultz P. Cryo-electron microscopy of vitrified specimens. Q. Rev. Biophys. 21 (1988) 129-228
28. Egelman E.H. Single-particle reconstruction from EM images of helical filaments. Curr. Opin. Struct. Biol. 17 (2007) 556-561
29. Egelman E.H., and Orlova A. New insights into actin filament dynamics. Curr. Opin. Struct. Biol. 5 (1995) 172-180
30. Eifler N., Vetsch M., Gregorini M., Ringler P., Chami M., Philippsen A., Fritz A., Müller S.A., Glockshuber R., Engel A., and Grauschopf U. Cytotoxin ClyA from Escherichia coli assembles to a 13-meric pore independent of its redox-state. EMBO J. 25 (2006) 2652-2661
31. Endres N.F., Yoshioka C., Milligan R.A., and Vale R.D. A lever-arm rotation drives motility of the minus-end-directed kinesin Ncd. Nature 439 (2006) 875-878
32. Fahrenkrog B., and Aebi U. The nuclear pore complex: nucleocytoplasmic transport and beyond. Nat. Rev. Mol. Cell Biol. 4 (2003) 757-766
33. Fahrenkrog B., Hubner W., Mandinova A., Pante N., Keller W., and Aebi U. The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import. Mol. Biol. Cell 11 (2000) 3885-3896
34. Fellmann D., Banez R., Carragher B., and Potter C.S. Temperature monitoring of an EM environment. Microsc. Today (2006) 24-28
35. Ford R.C., and Holzenburg A. Electron crystallography of biomolecules: mysterious membranes and missing cones. Trends Biochem. Sci. 33 (2008) 38-43
36. 2+-binding domain at the C terminus of AQP1. J. Mol. Biol. 318 (2002) 1381-1394
37. Frangakis A.S., Bohm J., Forster F., Nickell S., Nicastro D., Typke D., Hegerl R., and Baumeister W. Identification of macromolecular complexes in cryoelectron tomograms of phantom cells. Proc. Natl. Acad. Sci. USA 99 (2002) 14153-14158
38. Freitag B., Kujawa S., Mul P.M., Ringnalda J., and Tiemeijer P.C. Breaking the spherical and chromatic aberration barrier in transmission electron microscopy. Ultramicroscopy 102 (2005) 209-214
39. Fujiyoshi Y. The structural study of membrane proteins by electron crystallography. Adv. Biophys. 35 (1998) 25-80
40. Gao H., and Frank J. Molding atomic structures into intermediate-resolution cryo-EM density maps of ribosomal complexes using real-space refinement. Structure 13 (2005) 401-406
41. Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., and Frank J. Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement. Cell 113 (2003) 789-801
42. Giddings Jr. T.H., O'Toole E.T., Morphew M., Mastronarde D.N., McIntosh J.R., and Winey M. Using rapid freeze and freeze-substitution for the preparation of yeast cells for electron microscopy and three-dimensional analysis. Methods Cell Biol. 67 377 (2001) 27-42
43. Goldsbury C., Frey P., Olivieri V., Aebi U., and Müller S.A. Multiple assembly pathways underlie amyloid-beta fibril polymorphisms. J. Mol. Biol. 352 (2005) 282-298
44. Gonen T., Cheng Y., Sliz P., Hiroaki Y., Fujiyoshi Y., Harrison S.C., and Walz T. Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. Nature 438 (2005) 633-638
45. Gonen T., Cheng Y., Sliz P., Hiroaki Y., Fujiyoshi Y., Harrison Stephen C., and Walz T. Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. Nature 438 (2005) 633-638
46. Goodman J. Introduction to Fourier Optics (1968), McGraw-Hill Book Company, New York
47. Gregori L., Hainfeld J.F., Simon M.N., and Goldgaber D. Binding of amyloid β protein to the 20S proteasome. J. Biol. Chem. 272 (1997) 58-62
48. Guo, Z., Qin, T., Liao, B., Dong, W., 2006. Clamp for chemical staining treatment of scanning electron microscope test sample, 12 pp. (Shanghai Semiconductor Manufacturing International Corporation, Peop. Rep. China). Application: CN.
49. Hahn E., Wild P., Hermanns U., Sebbel P., Glockshuber R., Haner M., Taschner N., Burkhard P., Aebi U., and Müller S.A. Exploring the 3D molecular architecture of Escherichia coli type 1 pili. J. Mol. Biol. 323 (2002) 845-857
50. Hainfeld J.F., and Powell R.D. New frontiers in gold labeling. J. Histochem. Cytochem. 48 (2000) 471-480
51. Harris A., Belnap D.M., Watts N.R., Conway J.F., Cheng N., Stahl S.J., Vethanayagam J.G., Wingfield P.T., and Steven A.C. Epitope diversity of hepatitis B virus capsids: quasi-equivalent variations in spike epitopes and binding of different antibodies to the same epitope. J. Mol. Biol. 355 (2006) 562-576
52. He W., Kivork C., Machinani S., Morphew Mary K., Gail Anna M., Tesar Devin B., Tiangco Noreen E., McIntosh J.R., and Bjorkman P.J. A freeze substitution fixation-based gold enlarging technique for EM studies of endocytosed nanogold-labeled molecules. J. Struct. Biol. 160 (2007) 103-113
53. Henderson R., and Unwin P.N.T. Three-dimensional model of purple membrane obtained by electron microscopy. Nature 257 (1975) 28-32
54. Hess M.W. Cryopreparation methodology for plant cell biology. Methods Cell Biol. 79 (2007) 57-100
55. Hoenger A., and Gross H. Structural investigations into microtubule-map complexes. Methods Cell Biol. 84 (2008) 425-444
56. Kikkawa M. A new theory and algorithm for reconstructing helical structures with a seam. J. Mol. Biol. 343 (2004) 943-955
57. King M.E., Ghoshal N., Wall J.S., Binder L.I., and Ksiezak-Reding H. Structural analysis of Pick's disease-derived and in vitro-assembled tau filaments. Am. J. Pathol. 158 (2001) 1481-1490
58. Köhler R., Schafer K., Müller S., Vignon G., Diederichs K., Philippsen A., Ringler P., Pugsley AP., Engel A., and Welte W. Structure and assembly of the pseudopilin PulG. Mol. Microbiol. 54 (2004) 647-664
59. Koster A.J., Grimm R., Typke D., Hegerl R., Stoschek A., Walz J., and Baumeister W. Perspectives of molecular and cellular electron tomography. J. Struct. Biol. 120 (1997) 276-308
60. Krzysiak T.C., Wendt T., Sproul L.R., Tittmann P., Gross H., Gilbert S.P., and Hoenger A. A structural model for monastrol inhibition of dimeric kinesin Eg5. EMBO J. 25 (2006) 2263-2273
61. Lee K.K., and Johnson J.E. Complementary approaches to structure determination of icosahedral viruses. Curr. Opin. Struct. Biol. 13 (2003) 558-569
62. Lenzi D., Runyeon J.W., Crum J., Ellisman M.H., and Roberts W.M. Synaptic vesicle populations in saccular hair cells reconstructed by electron tomography. J. Neurosci. 19 (1999) 119-132
63. Lorentz M., Popp D., and Holmes K.C. Refinement of the F-actin model against X-ray fiber diffraction data by the use of a directed mutation algorithm. J. Mol. Biol. 234 (1993) 926-936
64. Maco B., Fahrenkrog B., Huang N.-P., and Aebi U. Nuclear pore complex structure and plasticity revealed by electron and atomic force microscopy. Methods Mol. Biol. 322 (2006) 273-288
65. Martin J., Goldie K.N., Engel A., and Hartl F.U. Topology of the morphological domains of the chaperonin GroEL visualized by immuno-electron microscopy. Biol. Chem. 375 (1994) 635-639
66. Masugi-Tokita M., and Shigemoto R. High-resolution quantitative visualization of glutamate and GABA receptors at central synapses. Curr. Opin. Neurobiol. 17 (2007) 387-393
67. McDonald K. Cryopreparation methods for electron microscopy of selected model systems. Methods Cell Biol. 79 (2007) 23-56
68. Metlagel Z., Kikkawa Y.S., and Kikkawa M. Ruby-Helix: an implementation of helical image processing based on object-oriented scripting language. J. Struct. Biol. 157 (2006) 95-105
69. Miyazawa A., Fujiyoshi Y., and Unwin N. Structure and gating mechanism of the acetylcholine receptor pore. Nature 423 (2003) 949-955
70. Morgelin M., Paulsson M., Heinegard D., Aebi U., and Engel J. Evidence of a defined spatial arrangement of hyaluronate in the central filament of cartilage proteoglycan aggregates. Biochem. J. 307 Pt 2 (1995) 595-601
71. Mueller C.A., Broz P., Müller S.A., Ringler P., Erne-Brand F., Sorg I., Kuhn M., Engel A., and Cornelis Guy R. The V-antigen of Yersinia forms a distinct structure at the tip of injectisome needles. Science 310 (2005) 674-676
72. Müller S.A., and Engel A. Structure and mass analysis by scanning transmission electron microscopy. Micron 32 (2001) 21-31
73. Müller S.A., and Engel A. Biological scanning transmission electron microscopy: imaging and single molecule mass determination. CHIMIA 60 (2006) 749-753
74. Murata K., Mitsuoka K., Hirai T., Walz T., Agre P., Heymann J.B., Engel A., and Fujiyoshi Y. Structural determinants of water permeation through aquaporin-1. Nature 407 (2000) 599-605
75. Nickell S., Kofler C., Leis A.P., and Baumeister W. A visual approach to proteomics. Nat. Rev. Mol. Cell Biol. 7 (2006) 225-230
76. Nickell S., Forster F., Linaroudis A., Net W.D., Beck F., Hegerl R., Baumeister W., and Plitzko J.M. Tom software toolbox: acquisition and analysis for electron tomography. J. Struct. Biol. 149 (2005) 227-234
77. Philippsen A., Engel H.-A., and Engel A. The contrast-imaging function for tilted specimens. Ultramicroscopy 107 (2007) 202-212
78. Philippsen A., Schenk A.D., Signorell G.A., Mariani V., Berneche S., and Engel A. Collaborative em image processing with the IPLT image processing library and toolbox. J. Struct. Biol. 157 (2006) 28-37
79. Phillips D.M. Electron microscopy: use of transmission and scanning electron microscopy to study cells in culture. Methods Cell Biol. 57 (1998) 297-311
80. Plancon L., Chami M., and Letellier L. Reconstruction of FhuA, an Escherichia coli outer membrane protein, into liposomes. Binding of phage t5 to FhuA triggers the transfer of DNA into the proteoliposomes. J. Biol. Chem. 272 (1997) 16868-16872
81. Rath B.K., and Frank J. Fast automatic particle picking from cryo-electron micrographs using a locally normalized cross-correlation function: a case study. J. Struct. Biol. 145 (2004) 84-90
82. Reichelt R., Holzenburg A., Buhle Jr. E.L., Jarnik M., Engel A., and Aebi U. Correlation between structure and mass distribution of the nuclear pore complex and of distinct pore complex components. J. Cell Biol. 110 (1990) 883-894
83. Rèmigy H.W., Caujolle-Bert D., Suda K., Schenk A., Chami M., and Engel A. Membrane protein reconstitution and crystallization by controlled dilution. FEBS Lett. 555 (2003) 160-169
84. Rizzoli S.O., and Betz W.J. Synaptic vesicle pools. Nat. Rev. Neurosci. 6 (2005) 57-69
85. Robenek H., and Severs N.J. Recent advances in freeze-fracture electron microscopy: the replica immunolabeling technique. Biol. Proced. Online 10 (2008) 9-19
86. Robertson C.E. Electron microscopy of Archaea. Methods Cell Biol. 79 (2007) 169-191
87. Roseman A.M. Docking structures of domains into maps from cryo-electron microscopy using local correlation. Acta Crystallogr. Sect. D Biol. Crystallogr. D56 (2000) 1332-1340
88. Sandblad L., Busch K.E., Tittmann P., Gross H., Brunner D., and Hoenger A. The Schizosaccharomyces pombe EB1 homolog Mal3p binds and stabilizes the microtubule lattice seam. Cell 127 (2006) 1415-1424
89. Segui-Simarro J.M., and Staehelin L.A. Cell cycle-dependent changes in golgi stacks, vacuoles, clathrin-coated vesicles and multivesicular bodies in meristematic cells of Arabidopsis thaliana: a quantitative and spatial analysis. Planta 223 (2006) 223-236
90. Severs N.J. Freeze-fracture electron microscopy. Nat. Protoc. 2 (2007) 547-576
91. Shaikh T.R., Thomas D.R., Chen J.Z., Samatey F.A., Matsunam H., Imada K., Namba K., and DeRosier D.J. A partial atomic structure or the flagellar hook of Salmonella typhimurium. Proc Natl. Acad. Sci. USA 102 (2005) 1023-1028
92. Shakil S.S., Holmer H.K., Moore C., Abernathy A.T., Jakowec M.W., Petzinger G.M., and Meshul C.K. High and low responders to novelty show differential effects in striatal glutamate. Synapse 58 (2005) 200-207
93. Sindelar C.V., and Downing K.H. The beginning of kinesin's force-generating cycle visualized at 9 Å resolution. J. Cell Biol. 177 (2007) 377-385
94. Skiniotis G., Surrey T., Altmann S., Gross H., Song Y.-H., Mandelkow E., and Hoenger A. Nucleotide-induced conformations in the neck region of dimeric kinesin. EMBO J. 22 (2003) 1518-1528
95. Suloway C., Pulokas J., Fellmann D., Cheng A., Guerra F., Quispe J., Stagg S., Potter Clinton S., and Carragher B. Automated molecular microscopy: the new Leginon system. J. Struct. Biol. 151 (2005) 41-60
96. Sousa A.A., Aronova M.A., Kim Y.C., Dorward L.M., Zhang G., and Leapman R.D. On the feasibility of visualizing ultrasmall gold labels in biological specimens by STEM tomography. J. Struct. Biol. 159 (2007) 507-522
97. Stagg S.M., Lander G.C., Pulokas J., Fellmann D., Cheng A., Quispe J.D., Mallick S.P., Avila R.M., Carragher B., and Potter C.S. Automated cryoEM data acquisition and analysis of 284742 particles of GroEL. J. Struct. Biol. 155 (2006) 470-481
98. Stagg S.M., Guerkan C., Fowler D.M., LaPointe P., Foss T.R., Potter C.S., Carragher B., and Balch W.E. Structure of the Sec13/31 COPII coat cage. Nature 439 (2006) 234-238
99. Steinmetz M.O., Stoffler D., Hoenger A., Bremer A., and Aebi U. Actin: from cell biology to atomic detail. J. Struct. Biol. 119 (1997) 295-320
100. Steinmetz M.O., Stoffler D., Müller S.A., Jahn W., Wolpensinger B., Goldie K.N., Engel A., Faulstich H., and Aebi U. Evaluating atomic models of F-actin with an undecagold-tagged phalloidin derivative. J. Mol. Biol. 276 (1998) 1-6
101. Steven A.C., Heymann J.B., Cheng N., Trus B.L., and Conway J.F. Virus maturation: dynamics and mechanism of a stabilizing structural transition that leads to infectivity. Curr. Opin. Struct. Biol. 15 (2005) 227-236
102. Stoffler D., Feja B., Fahrenkrog B., Walz J., Typke D., and Aebi U. Cryo-electron tomography provides novel insights into nuclear pore architecture: implications for nucleocytoplasmic transport. J. Mol. Biol. 328 (2003) 119-130
103. Takamori S., Holt M., Stenius K., Lemke E.A., Gronborg M., Riedel D., Urlaub H., Schenck S., Bruegger B., Ringler P., Müller S.A., Rammner B., Graeter F., Hub J.S., De Groot B.L., Mieskes G., Moriyama Y., Klingauf J., Grubmüller H., Heuser J., Wieland F., and Jahn R. Molecular anatomy of a trafficking organelle. Cell 127 (2006) 831-846
104. Traxler K.W., Norcum M.T., Hainfeld J.F., and Carlson G.M. Direct visualization of the calmodulin subunit of phosphorylase kinase via electron microscopy following subunit exchange. J. Struct. Biol. 135 (2001) 231-238
105. Unwin N. Refined structure of the nicotinic acetylcholine receptor at 4 Å resolution. J. Mol. Biol. 346 (2005) 967-989
106. von Bergen M., Barghorn S., Müller S.A., Pickhardt M., Biernat J., Mandelkow E.-M., Davies P., Aebi U., and Mandelkow E. The core of tau-paired helical filaments studied by scanning transmission electron microscopy and limited proteolysis. Biochemistry 45 (2006) 6446-6457
107. Wang Y.A., Yu X., Yip C., Strynadka N.C., and Egelman E.H. Structural polymorphism in bacterial EspA filaments revealed by cryo-EM and an improved approach to helical reconstruction. Structure 14 (2006) 1189-1196
108. Wang Y.A., Yu X., Overman S., Tsuboi M., Thomas G.J., and Egelman E.H. The structure of a filamentous bacteriophage. J. Mol. Biol. 361 (2006) 209-215
109. Wickert U., Mücke N., Wedig T., Müller S.A., Aebi U., and Herrmann H. Characterization of the in vitro co-assembly process of the intermediate filament proteins vimentin and desmin: mixed polymers at all stages of assembly. Eur. J. Cell Biol. 84 (2005) 379-391
110. Winey M., Yarar D., Giddings Jr. T.H., and Mastronarde D.N. Nuclear pore complex number and distribution throughout the Saccharomyces cerevisiae cell cycle by three-dimensional reconstruction from electron micrographs of nuclear envelopes. Mol. Biol. Cell 8 (1997) 2119-2132
111. Yakushevska A.E., Lebbink M.N., Geerts W.J.C., Spek L., van Donselaar E.G., Jansen K.A., Humbel B.M., Post J.A., Verkleij A.J., and Koster A.J. STEM tomography in cell biology. J. Struct. Biol. 159 (2007) 381-391
112. Yonekura K., Maki-Yonekura S., and Namba K. Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy. Nature 424 (2003) 643-650
113. Young R.J., and Moore M.V. Dual-beam (FIB-SEM) systems. Techniques and automated applications. In: Giannuzzi L.A., and Stevie F. (Eds). Introduction to Focused Ion Beams (2005), Springer Science + Business Media Inc., New York 247-268