Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy

Journal of Structural Biology

Volumn 150, Issue 1, 2005, Pages 109-121

  Al Amoudi, Ashraf ^a   Studer, Daniel ^b   Dubochet, Jacques ^a  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

CEMOVIS; Chatter; Compression; Crevasses; Cutting artefacts; Knife marks

Indexed keywords

ANALYTIC METHOD; ARTICLE; ARTIFACT; COMPRESSION; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; FLOW; FRICTION; IMAGE QUALITY; MODEL; NONHUMAN; PARAMETER; PRIORITY JOURNAL; QUALITATIVE ANALYSIS;

EID: 15444366581     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jsb.2005.01.003     Document Type: Article

References (37)

1. A. Al-Amoudi, J. Dubochet, H. Gnaegi, W. Lüthi, and D. Studer An oscillating cryo-knife reduces cutting-induced deformation of vitreous ultrathin sections J. Microsc. 212 2003 26 33
2. A. Al-Amoudi, L. Norlén, and J. Dubochet Cryo-electron microscopy of vitreous sections of native biological cells and tissues J. Struct. Biol. 148 2004 131 135
3. Al-Amoudi, A., Dubochet, J., Norlén, L., 2005. Nanostructure of the epidermal extracellular space as observed by cryo-electron microscopy of vitreous sections of human skin. J. Invest Dermatol. (in press)
4. A. Al-Amoudi, J.J. Chang, A. Leforestier, A. McDowall, L. Salamin, L. Norlén, K. Richter, N. Sartori Blanc, D. Studer, and J. Dubochet Cryo-electron microscopy of vitreous sections EMBO J. 23 2004 3583 3588
5. A. Al-Amoudi, J. Dubochet, and D. Studer Amorphous solid water produced by cryosectioning of crystalline ice at 113K J. Microsc. 207 2002 146 153
6. C.A. Angell Amorphous water Ann. Rev. Phys. Chem. 55 2004 559 583
7. J.J. Chang, A.W. McDowall, J. Lepault, R. Freeman, C.A. Walter, and J. Dubochet Freezing, sectioning and observation artefacts of frozen hydrated sections for electron microscopy J. Microsc. 132 1983 109 123
8. R.M. Christensen Theory of viscoelasticity 1982 Academic Press New York
9. J. Dubochet, and N. Sartori Blanc The cell in absence of aggregation artifacts Micron 32 2001 91 99
10. J. Dubochet, and A.W. McDowall Frozen hydrated sections Science of Biological Specimen Preparation 1984 147 152
11. J. Dubochet, M. Adrian, J.-J. Chang, J.-C. Homo, J. Lepault, A.W. McDowall, and P. Schultz Cryo-electron microscopy of vitrified specimens Q. Rev. Biophys. 21 1988 129 228
12. P.M. Frederik, W.M. Busing, and A. Persson Surface defects on thin cryosections Scanning Electron Microsc. 1 1984 433 443
13. G. Griffiths, A. McDowall, R. Back, and J. Dubochet On the preparation of cryosections for immunocytochemistry J. Ultrastruct. Res. 89 1984 65 84
14. C.-E. Hsieh, M. Marko, J. Frank, and C.A. Mannella Electron tomographic analysis of frozen-hydrated tissue sections J. Struct. Biol. 138 2002 63 73
15. J.-C. Jésior How to avoid compression II. The influence of sectioning conditions J. Ultrastruc. Mol. Struct. Res. 95 1986 210 217
16. A. Leforestier, J. Dubochet, and F. Livolant Bilayers of nucleosome core particles Biophys. J. 81 2001 2414 2421
17. K.G. Lickfeld Ein Betrag zur Frage welche Kräfte und Faktoren Düunnstschneiden bewirken J. Ultrastruct. Res. 93 1985 101 115
18. V.R.F. Matias, A. Al-Amoudi, J. Dubochet, and T.J. Beveridge Cryo-transmission electron microscopy of frozen-hydrated sections of Escherichia coli and Pseudomonas aeruginosa J. Bacteriol. 185 2003 6112 6118
19. T.R. Matzelle, H. Gnaegi, A. Ricker, and R. Reichelt Characterization of the cutting edge of glass and diamond knives for ultramicrotomy by scanning force microscopy using cantilevers with a defined tip geometry Part II J. Microsc. 209 2003 113 117
20. A.W. McDowall, J.-J. Chang, R. Freeman, J. Lepault, C.A. Walter, and J. Dubochet Electron microscopy of frozen hydrated sections of vitreous ice and vitrified biological samples J. Microsc. 131 1983 1 9
21. L. Norlén, and A. Al-Amoudi Stratum corneum keratin structure, function, and formation: The cubic rod-packing and membrane templating model J. Invest. Dermatol. 123 2004 715 732
22. L. Norlén, A. Al-Amoudi, and J. Dubochet A cryotransmission electron microscopy study of skin barrier formation J. Invest. Dermatol. 120 2003 555 560
23. T. Özel, and T. Altan Determination of workpiece flow stress and friction at the chip-tool contact for high-speed cutting Int. J. Mach. Tools Manu. 40 2000 133 152
24. K. Richter, H. Gnägi, and J. Dubochet A model for cryosectioning based on the morphology of vitrified ultrathin sections J. Microsc. 163 1991 19 28
25. K. Richter A cryoglue to mount vitreous biological specimens for cryoultramicrotomy at 110K J. Microsc. 173 1994 143 147
26. K. Richter Cutting artefacts on ultrathin cryosections of biological bulk specimens Micron 25 1994 297 308
27. K. Richter High-density morphologies of ice in high-pressure frozen biological specimens Ultramicroscopy 53 1994 237 249
28. N. Sartori Blanc, A. Senn, A. Leforestier, F. Livolant, and J. Dubochet DNA in human and stallion spermatozoa forms local hexagonal packing with twist and many defects J. Struct. Biol. 134 2001 76 81
29. N. Sartori, K. Richter, and J. Dubochet Vitrification depth can be increased more than 10-fold by high pressure freezing J. Microsc. 172 1993 55 61
30. N. Sartori Blanc, D. Studer, K. Ruhl, and J. Dubochet Electron beam-induced changes in vitreous sections of biological samples J. Microsc. 192 1998 194 201
31. H. Sitte Advanced instrumentation and methodology related to cryoultramicrotomy: A review Scanning Microsc. Suppl. 10 1996 387 466
32. D. Studer, W. Graber, A. Al-Amoudi, and P. Eggli A new approach for cryofixation by high-pressure freezing J. Microsc. 203 2001 285 294
33. K.T. Tokuyasu, and S.J. Singer Improved procedures for immunoferritin labeling of ultrathin frozen sections J. Cell Biol. 71 1976 894 906
34. M. Urbakh, J. Klafter, and J. Israelachvili The nonlinear nature of friction Nature 430 2004 525 528
35. P. Zhang, E. Bos, J. Heymann, H. Gnaegi, M. Kessel, P.J. Peters, and S. Subramaniam Direct visualization of receptor arrays in frozen-hydrated sections and plunge-frozen specimens of E. coli engineered to overproduce the chemotaxis receptor Tsr J. Microsc. 216 2004 76 83
36. K. Zierold The morphology of ultrathin cryosections Ultramicroscopy 14 1984 201 210
37. K. Zierold Cryoultramicrotomy R.A. Steinbrecht K. Zierold Cryotechniques in biological electron microscopy 1987 Springer Berlin, Heidelberg 132 148