Cryo-electron microscopy of vitreous sections

EMBO Journal

Volumn 23, Issue 18, 2004, Pages 3583-3588

  Al Amoudi, Ashraf ^a   Chang, Jiin Ju ^b   Leforestier, Amélie ^c   McDowall, Alasdair ^d   Salamin, Laurée Michel ^a   Norlén, Lars P O ^e   Richter, Karsten ^f   Blanc, Nathalie Sartori ^a   Studer, Daniel ^g   Dubochet, Jacques ^a  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^c UNIVERSITÉ PARIS SUD   (France)

^d UNIVERSITY OF QUEENSLAND   (Australia)

^e UNIVERSITY OF GENEVA   (Switzerland)

^f GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^g UNIVERSITY OF BERN   (Switzerland)

Author keywords

Bacterial envelope; Desmosome; High pressure freezing; Intermediate filament; Vitrification

Indexed keywords

BIOMATERIAL;

ARTIFACT; COMPUTER ASSISTED TOMOGRAPHY; CRYOELECTRON MICROSCOPY; FILM; HYDRATION; IMAGE RECONSTRUCTION; PRIORITY JOURNAL; REVIEW; STAINING; SUSPENSION;

BACTERIA (MICROORGANISMS);

EID: 20844438496     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.emboj.7600366     Document Type: Review

References (43)

1. Abbott A (2002) The society of proteins. Nature 417: 894-896
2. Adrian M, Dubochet J, Lapault J, McDowall AW (1984) Cryo-electron microscopy of viruses. Nature 308: 32-36
3. Al-Amoudi A, Dubochet J, Gnaegi H, Lüthi W, Studer D (2003) An oscillating cryo-knife reduces cutting-induced deformation of vitreous ultrathin sections. J Microsc 212: 26-33
4. Al-Amoudi A, Dubochet J, Studer D (2002) Amorphous solid water produced by cryosectioning of crystalline ice at 113 K. J Microsc 207: 146-153
5. Al-Amoudi A, Norlén LPO, Dubochet J (2004) Cryo-electron microscopy of vitreous sections of native biological cells and tissues. J Struct Biol (in press)
6. Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P (2002) Molecular Biology of the Cell. New York, London: Garland Publishing Inc.
7. Angell CA (2004) Amorphous water. Annu Rev Phys Chem 55: 559-583.
8. Beveridge TJ (1999) Structures of Gram-negative cell walls and their derived membrane vesicles. J Bacteriol 181: 4725-4733
9. Dubochet J, Adrian M, Chang J-J, Homo J-C, Lepault J, McDowall AW, Schultz P (1988) Cryo-electron microscopy of vitrified specimens. Q Rev Biophys 21: 129-228
10. Dubochet J, Bednar J, Furrer P, Stasiak A (1994) Cryo-electron microscopy of DNA. In Nucleic Acids and Molecular Biology, Eckstein F, Lilley DMJ (eds) Vol. 8, pp 41-55. Berlin: Springer-Verlag
11. Dubochet J, McDowall AW (1981) Vitrification of pure water for electron microscopy. J Microsc 124: RP3-RP4
12. Dubochet J, Sartori Blanc N (2001) The cell in absence of aggregation artifacts. Micron 32: 91-99
13. Fernandez-Moran H (1985) Cryo-electron microscopy and ultramicrotomy: reminiscences and reflections. In Adv Electronics Electron Phys. Academic Press suppl. 16, pp 167-223
14. Garrod DR, Merritt AJ, Nie Z (2002) Desmosomal cadherins. Curr Opin Cell Biol 14: 537-545
15. Gautier A, Michel-Salamin L, Tosi-Couture E, McDowall AW, Dubochet J (1986) Electron microscopy of the chromosomes of dinoflagellates in situ: confirmation of Bouligand's liquid crystal hypothesis. J Ultrastruct Mol Struct Res 97: 10-30
16. Grünewald K, Medalia O, Gross A, Steven AC, Baumeister W (2003) Prospects of electron cryotomography to visualize macromolecular complexes inside cellular compartments: implications of crowding. Biophys Chem 100: 577-591
17. He W, Cowin P, Stokes DL (2003) Untangling desmosomal knots with electron tomography. Science 302: 109-113
18. Hegerl R, Hoppe W (1976) Influence of electron noise on three-dimensional reconstruction. Z Naturforsch 31a: 1717-1721
19. Hoiczyk E, Baumeister W (1995) Envelope structure of four gliding filamentous cyanobacteria. J Bacterial 177: 2387-2395
20. Leforestier A, Dubochet J, Livolant F (2001) Bilayers of nucleosome core particles. Biophys J 81: 2414-2421
21. Lepault J, Ranck J-L, Erk I (1992) Electron cryomicroscopy of frozen-hydrated biological specimens: analysis of freezing artifacts by X-ray cryocrystallography. Ultramicroscopy 46: 19-24
22. Matias VRF, Al-Amoudi A, Dubochet J, Beveridge TJ (2003) Cryotransmission electron microscopy of frozen-hydrated sections of Gram-negative bacteria. J Bacteriol 185: 6112-6118
23. Mayer E, Brüggeller P (1980) Complete vitrification in pure liquid water and dilute aqueous solutions. Nature 288: 569-571
24. McDowall AW, Smith JM, Dubochet J (1986) Cryo-electron microscopy of vitrified chromosomes in situ. EMBO J 5: 1395-1402
25. Medalia O, Weber I, Frangakis AS, Nicastro D, Gerisch G, Baumeister W (2002) Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography. Science 298: 1209-1213
26. Michel M, Hillmann T, Müller M (1991) Cryosectioning of plant material frozen at high pressure. J Microsc 163: 13-18
27. Moor H (1987) Theory and practice of high pressure freezing. In Cryotechniques in Biological Electron Microscopy, Steinbrecht RA, Zierold K (eds) pp 175-191. Heidelberg: Springer
28. Nicastro D, Frangakis AS, Typke D, Baumeister W (2000) Cryoelectron tomography of Neurospora mitochondria. J Struct Biol 129: 48-56
29. Norlén L, Al-Amoudi A (2004) Stratum corneum keratin structure, function and formation-the cubic rod-packing and membrane templating model. J Invest Dermatol (in press)
30. Norlén L, Al-Amoudi A, Dubochet J (2003) A cryotransmission electron microscopy study of skin barrier formation. J Invest Dermatol 120: 555-560
31. Parry DA, Steinert PM (1999) Intermediate filaments: molecular architecture, assembly, dynamics and polymorphism. Q Rev Biophys 32: 99-187
32. Richter K (1994a) A cryoglue to mount vitreous biological specimens for cryoultramicrotomy at 110K. J Microsc 173: 143-147
33. Richter K (1994b) Cutting artefacts on ultrathin cryosections of biological bulk specimens. Micron 25: 297-308
34. Richter K (1994c) High-density morphologies of ice in high-pressure frozen biological specimens. Ultramicroscopy 53: 237-249
35. Richter K, Gnägi H, Dubochet J (1991) A model for cryosectioning based on the morphology of vitrified ultrathin sections. J Microsc 163: 19-28
36. Robinow C, Kellenberger E (1994) The bacterial nucleoid revisited. Microbiol Rev 58: 211-232
37. Sali A, Glaeser R, Earnest T, Baumeister W (2003) From words to literature in structural proteomics. Nature 422: 216-225
38. Sartori Blanc N, Senn A, Leforestier A, Livolant F, Dubochet J (2001) DNA in human and stallion spermatozoa forms local hexagonal packing with twist and many defects. J Struct Biol 134: 76-81
39. Sartori Blanc N, Studer D, Ruhl K, Dubochet J (1998) Electron beam-induced changes in vitreous sections of biological samples. J Microsc 192: 194-201
40. Sartori N, Richter K, Dubochet J (1993) Vitrification depth can be increased more than 10 fold by high pressure freezing. J Microsc 172: 55-61
41. Sleytr UB, Beveridge TJ (1999) Bacterial S-layers. Trends Microbiol 7: 253-260
42. Studer D, Graber W, Al-Amoudi A, Eggli P (2001) A new approach for cryofixation by high-pressure freezing. J Microsc 203: 285-294
43. Studer D, Michel M, Müller M (1989) High pressure freezing comes of age. Scanning Microsc 3: 253-269