Polymerisation of chemically cross-linked actin:thymosin β4 complex to filamentous actin: Alteration in helical parameters and visualisation of thymosin β4 binding on F-actin

Journal of Molecular Biology

Volumn 315, Issue 4, 2002, Pages 613-625

  Ballweber, Edda ^a   Hannappel, Ewald ^b   Huff, Thomas ^b   Stephan, Harald ^a   Haener, Markus ^c   Taschner, Nicole ^c   Stoffler, Daniel ^c   Aebi, Ueli ^c   Mannherz, Hans Georg ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

^b UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

^c UNIVERSITY OF BASEL   (Switzerland)

Author keywords

Actin; DNase I; Gelsolin; Phalloidin; thymosins

Indexed keywords

ACTIN; F ACTIN; GELSOLIN; PHALLOIDIN; THYMOSIN BETA4;

ARTICLE; BINDING SITE; COMPLEX FORMATION; CROSS LINKING; MOLECULAR WEIGHT; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PUBLISHING; STAINING; TRANSMISSION ELECTRON MICROSCOPY;

EID: 0036305697     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.5281     Document Type: Article

References (42)

1. Isolation of a 5 kilodalton actin-sequestering peptide from human blood platelets
2. 4 and Fx an actin sequestering peptide are indistinguishable
3. Structure of actin binding proteins: Insights about function at atomic resolution
4. β-Thymosins, small acidic peptides with multiple functions
5. Beta thymosins as actin binding peptides
6. 4 binds actin in an extended conformation and contacts both the barbed and pointed ends
7. 4 on actin by competition with G-actin binding proteins indicates negative cooperativity between binding sites located on subdomains 1 and 2 of actin
8. Plant profilin induces actin polymerisation from actin:thymosin complexes and competes directly with β-thymosins and with negative co-operativity with DNase I for binding to actin
9. 4 complex by calcium, phalloidin, free ends of filamentous actin, skeletal muscle subfragment 1 and chicken intestinal myosin I
10. 4 is not a simple G-actin sequestering protein and interacts with F-actin at high concentration
11. β-Thymosins are not simple actin monomer buffering proteins
12. 4 serves as a glutaminyl substrate of transglutaminase. Labelling with fluorescent dansylcadaverine does not abolish interaction with G-actin
13. 4 like peptides as assessed by the DNase I inhibition assay
14. Computer generated Fourier transforms of helical particles
15. Cofilin changes the twist of F-actin: Implications for actin filament dynamics and cellular function
16. The atomic structure of actin:DNase I complex
17. Refinement of the F-actin model against X-ray fibre diffraction data by the use of a directed mutation algorithm
18. Evaluating atomic models of F-actin with an undecagold-tagged phalloidin derivative
19. 4 changes the conformation and dynamics of actin monomers
20. 4: A nuclear magnetic resonance and simulated annealing study
21. 4 in water determined by NMR spectroscopy
22. The regulation of rabbit skeletal muscle contraction. I: Biochemical studies on the interaction of the tropomyosin/troponin complex with actin and the proteolytic fragments of myosin
23. Expression of human plasma gelsolin in Escherichia coli and dissection of actin binding sites by segmental deletion mutagenesis
24. The interaction of bovine pancreatic deoxyribonuclease I and skeletal muscle actin
25. Met from pork spleen
26. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
27. Subtilisin-cleaved actin: Polymerisation and interaction with myosin subfragment 1
28. Purification and characterization of subtilisin cleaved actin lacking the segment of residues 43 to 47 in the DNase I binding loop
29. Monoclonal antibodies prepared against Dictyostelium actin: Characterization and interactions with actin
30. A simple procedure to produce monospecific polyclonal antibodies of high affinity against actin
31. Crystalline deoxyribonuclease I. Isolation and general properties
32. A specific actin:DNAase I complex formed by the action of DNAase I on F-actin
33. Factors influencing the precision of quantitative scanning transmission electron microscopy
34. Application of scanningtransmission electron microscopy to the study of biological structure
35. A correlative analysis of actin filament assembly, structure and dynamics
36. Semper: Distortion compensation, selective averaging, 3-D reconstruction, and transfer function correction in a highly programmable system
37. The micrograph data processing program
38. Studies on the structure of the bacteriophage T4 tail sheath. I. The recovery of 3-D structural information from the extended sheath
39. Towards atomic interpretation of F-actin filament three-dimensional reconstructions
40. Reconstruction of three-dimensional images from electron micrographs of structures with helical symmetry
41. Real-time isocontouring and texture mapping meet new challenges in interactive molecular graphics applications