Recent advances in freeze-fracture electron microscopy: The replica immunolabeling technique

Biological Procedures Online

Volumn 10, Issue 1, 2008, Pages 9-19

  Robenek, Horst ^a   Severs, Nicholas J ^b  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

Author keywords

Freeze fracturing; Immunoelectron; Immunogold techniques; Microscopy

Indexed keywords

CARBON; DODECYL SULFATE SODIUM; FAT DROPLET; PLATINUM;

ANTIBODY LABELING; ARTICLE; CYTOCHEMISTRY; ELECTRON MICROSCOPY; FREEZE FRACTURE; FREEZE FRACTURE ELECTRON MICROSCOPY; IMMUNOGOLD LABELING; MEMBRANE STRUCTURE; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

EID: 38649132870     PISSN: 14809222     EISSN: None     Source Type: Journal    
DOI: 10.1251/bpo138     Document Type: Article

References (27)

1. Severs NJ. Freeze-fracture electron microscopy. Nature Protocols 2007;2:547-576.
2. Severs NJ. Freeze-fracture cytochemistry: an explanatory survey of methods. In "Rapid Freezing, Freeze Fracture, and Deep Etching" (NJ Severs, DM Shotton eds.), 1995, pp. 173-208. Wiley-Liss, New York.
3. Fujimoto K. Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane proteins - Application to the immunogold labeling of intercellular junctional complexes. J Cell Sci 1995;108:3443-3449.
4. Fujimoto K. SDS-digested freeze-fracture replica labeling electron microscopy to study the two-dimensional distribution of integral membrane proteins and phospholipids in biomembrane: practical procedure, interpretation and application. Histochem Cell Biol 1997; 107:87-96.
5. Pinto da Silva P, Kan FWK. Label-fracture: a method for high resolution labeling of cell surfaces. J Cell Biol 1984;99:1156-1161.
6. Andersson Forsman C, Pinto da Silva P. Label-fracture of cell surfaces by replica staining. J Histochem Cytochem 1998;36:1413-1418.
7. Pinto da Silva P. Visual thinking of biological membranes: from freeze-etching to label-fracture. In "Immunogold labeling methods in cell biology" (A Verkleij, JLN Leunissen eds.), 1989, pp. 179-197. CRC Press, Boca Raton.
8. Pinto da Silva P, Andersson Forsman C, Fujimoto K. Fracture-flip: nanoanatomy and topochemistry of cell surfaces. In "Cells and tissues: a three-dimensional approach by modern techniques in microscopy" (P Motta ed.), 1989, pp. 49-56. Alan R. Liss, New York.
9. Kan FWK, Pinto da Silva P. Label-fracture cytochemistry. In "Colloidal Gold. Principles, Methods and Applications" (MA Hayat ed.), 1989, pp. 175-201. Academic Press, Orlando.
10. Rash JE, Yasumura T, Hudson CS, Agre P, Nielsen S. Direct immunogold labeling of aquaporin-4 in square arrays of astrocyte and ependymocyte plasma membranes in rat brain and spinal cord. Proc Natl Acad Sci USA 1998;95:11981-11986.
11. Rash JE, Yasumura T. Direct immunogold labeling of connexins and aquaporin-4 in freeze-fracture replicas of liver, brain and spinal cord: factors limiting quantitative analysis. Cell Tiss Res 1999;296:307-321.
12. Rash JE, Olson CO, Pouliot WA, Davidson KGV, Yasumura T, Furman CS, Royer S, Kamasawa N, Nagy JI, Dudok FE. Connexin35 vs Connexin32, "Miniature" neuronal gap junctions and limited electrotonic coupling in rodent suprachiasmatic nucleus. Neuroscience 2007; 149:350-371.
13. Dunia I, Recouvreur M, Nicolas P, Kumar NM, Bloemendal H, Benedetti EL. Sodium dodecyl Sulphate-freeze-fracture immunolabeling of gap junctions. In "Methods in Molecular Biology: Connexin Methods and Protocols, Vol 154" (R Bruzzone, C Giaume eds.), 2001, pp. 33-55. Humana Press, Totowa.
14. Kamasawa N, Furman CS, Davidson KG, Sampson JA, Magnie AR, Gebhardt BR, Kamasawa M, Yasumura T, Zumbrunnen JR, Pickard GE, Nagy JI, Rash JE. Abundance and ultrastructural diversity of neuronal gap junctions in the OFF and ON sublaminae of the inner plexiform layer of rat and mouse retina. Neuroscience 2006;142-1093-1117.
15. Kamasawa N, Sik A, Morita M, Yasumura T, Davidson KG, Nagy JI, Rash SE. Connexin-47 and connexin-32 in gap junctions of oligodendrocyte somata, myelin sheaths, paranodal loops and Schmidt-Lanterman incisures: implications for ionic homeostasis and potassium siphoning. Neuroscience 2005;136:65-86.
16. Nagy JI, Dudek FE, Rash JE. Update on connexins and gap Junctions in neurons and glia in the mammalian nervous system. Brain Res Rev 2004;47:191-215.
17. Rash JE, Davidson KG, Kamasawa N, Yasumura T, Kamasawa M, Zhang C, Michaels R, Restrepo D, Ottersen OP, Olson CO, Nagy JI. Ultrastructural localization of connexins (Cx36, Cx43, Cx45), glutamate receptors and aquaporin-4 in rodent olfactory mucosa, olfactory nerve and olfactory bulb. J Neurocytol 2005;34:307-341.
18. Rash JE, Johnson TJA, Dinchuk JE, and Levinson SR. Labeling intramembrane particles in freeze-fracture replicas. In "Freeze-fracture Studies of Membranes" (SW. Hui ed.), 1989, pp. 41-59. CRC Press, Boca Raton.
19. Stevenson S, Rothery S, Cullen MJ, Severs NJ. Spatial relationship of C-terminal domains of dystrophin and β-dystroglycan in cardiac muscle support a direct molecular interaction at the plasma membrane interface. Circ Res 1998;82:82-93.
20. Stevenson SA, Cullen MJ, Rothery S, Coppen SR, Severs NJ. High-resolution en-face visualization of the cardiomyocyte plasma membrane reveals distinctive distributions of spectrin and dystrophin. Eur J Cell Biol 2005;84:961-971.
21. Robenek H, Hofnagel O, Buers I, Robenek MJ, Troyer D, Severs NJ. Adipophilin-enriched domains in the ER membrane are sites of lipid droplet biogenesis. J Cell Sci 2006;119:4215-4224.
22. Robenek MJ, Schlattmann K, Zimmer KP, Plenz G, Troyer D, Robenek H. Cholesterol transporter caveolin-1 transits the lipid bilayer during intracellular cycling. FASEB J 2003;17:1940-1942.
23. Robenek MJ, Severs NJ, Schlattmann Y, Plenz G, Zimmer KP, Troyer D, Robenek H. Lipids partition caveolin-1 from ER membranes into lipid droplets: updating the model of lipid droplet biogenesis. FASEB J 2004;18:866-868.
24. Robenek H, Lorkowski S, Schnoor M, Troyor D. Spatial integration of TIP47 and adipophilin in macrophage lipid bodies. J Biol Chem 2005;280:5789-5794.
25. Robenek H, Robenek MJ, Buers I, Lorkowski S, Hofnagel O, Troyer D, Severs NJ. Lipid droplets gain PAT family proteins by interaction with specialized plasma membrane domains. J Biol Chem 2005;280:26330-26338.
26. Robenek H, Hofnagel O, Buers I, Lorkowski S, Schnoor M, Robenek MJ, Heid H, Troyer D, Severs NJ. Butyrophilin controls milk fat globule secretion. Proc Natl Acad Sci USA 2006;103:10385-10390.
27. Branton D, Bullivant S, Gilula NB, Karnovsky MJ, Moor H, Muhlethaler K, Northcote DH, Packer L, Satir B, Satir P. Speth V, Staehelin LA, Steere RL, Weinstein RS. Freeze-etching nomenclature. Science 1975;190:54-56.