Structure and mass analysis by scanning transmission electron microscopy

Micron

Volumn 32, Issue 1, 2001, Pages 21-31

  Muller S A ^a   Engel, A ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

Author keywords

Electron beam sensitivity; Mass measurement; Scanning transmission electron microscope

Indexed keywords

ANALYTICAL PROCEDURES AND TECHNIQUES; ELECTRON BEAM; PROTEIN STRUCTURE; STRUCTURE ANALYSIS; TRANSMISSION ELECTRON MICROSCOPY;

CHAPERONINS; ESCHERICHIA COLI; MICROSCOPY, ELECTRON, SCANNING; SPLICEOSOMES;

EID: 0035239217     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0968-4328(00)00022-6     Document Type: Article

References (79)

1. Characterization of a functional GroEL(14)(GroES(7))(2) chaperonin hetero-oligomer
2. Visualization of a ternary complex of the Escherichia-Coli Phe-transfer RNA(Phe) and Tu.GTP from Thermus-Thermophilus by scanning transmission electron microscopy
3. Concentration evaluation of chromatin in unstained resin-embedded sections by means of low-dose ratio-contrast imaging in STEM
4. A polypeptide bound by the chaperonin GroEL is localized within a central cavity
5. The crystal structure of the bacterial chaperonin GroEL at 2.8 Angstrom
6. Towards atomic interpretation of F-actin filament three-dimensional reconstructions
7. Quantitative scanning transmission electron microscopy of ultrathin cryosections - Subcellular organelles in rapidly frozen liver and cerebellar cortex
8. The reproducible observation of unstained embedded cellular material in thin sections: Visualisation of an integral membrane protein by a new mode of imaging for STEM
9. Location of a folding protein and shape changes in GroEL-GroES complexes imaged by cryo-electron microscopy
10. Molecular microscopy of labeled polynucleotides: Stability of osmium atoms
11. Scanning transmission electron microscopy of biological structures
12. The current state of high resolution scanning electron microscopy
13. High-resolution scanning transmission electron microscopy
14. A Scanning microscope with 5 Å resolution
15. Characterisation of complexes between reca protein and duplex DNA by electron microscopy
16. Thickness dependence of the STEM ratio image
17. Molecular weight determination by scanning transmission electron microscopy
18. Imaging of biological structures with the scanning transmission electron microscope
19. Application of scanning transmission electron microscopy to the study of biological structure
20. A comparison of calculated images by six modes of transmission electron microscopy
21. Some progress in the use of a scanning transmission electron microscope for the observation of biomacromolecules
22. Digital acquisition and processing of electron micrographs using a scanning transmission electron microscope
23. Mass mapping of a protein complex with the scanning transmission electron microscope
24. Functional significance of symmetrical versus asymmetrical GroEL-GroES chaperonin complexes
25. Molecular mass determination by STEM and EFTEM: A critical comparison
26. Function in protein folding of TRiC, a cytosolic ring complex containing TCP-1 and structurally related subunits
27. Site specific cluster labels
28. Mapping the domains of molecules and complexes by mass and heavy atom loading
29. 2-discharged carbon film and characterized by molecular weight and its projected distribution
30. Identification and electron microscopic analysis of a chaperonin oligomer from Neurospora crassa mitochondria
31. Structural and mass analysis of dynein by scanning transmission electron microscopy
32. Z-contrast in biology
33. The influence of the surface relief of thin sections of embedded, unstained biological material on image quality
34. The biochemistry of pre-mRNA splicing
35. Chaperonin-mediated protein folding - GroES Binds to one end of the GroEL cylinder, which accommodates the protein substrate within its central cavity
36. Characterization of biological macromolecules by combined mass mapping and electron energy-loss spectroscopy
37. Compositional imaging with electron energy loss spectroscopy
38. Phosphorylation and subunit organization of axonal neurofilaments determined by scanning transmission electron microscopy
39. Conformational analysis of 16S ribosomal RNA from Escherichia coli by scanning transmission electron microscopy
40. Protein-induced conformational changes in 16 S ribosomal RNA during the initial assembly steps of the Escherichia coli 30 S ribosomal subunit
41. Visualization of ion-dependent conformational changes in E. coli 23S ribosomal RNA by scanning transmission electron microscopy
42. Topology of the morphological domains of the chaperonin GroEL visualized by immuno-electron microscopy
43. The C-terminal domain of RNA polymerase II couples mRNA processing to transcription
44. A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix
45. Identification and mass analysis of human fibrinogen molecules and their domains by scanning transmission electron microscopy
46. Factors influencing the precision of quantitative scanning transmission electron microscopy
47. A supraspliceosome model for large nuclear ribonucleo-protein particles based on mass determinations by scanning transmission electron microscopy
48. Structure of Tobacco Mosaic Virus at 3.6 Å resolution: Implications for assembly
49. Quantitative structural analysis of eukaryotic ribosomal RNA by scanning transmission electron microscopy
50. Tetrabrachion: A filamentous archaebacterial surface protein assembly of unusual structure and extreme stability
51. Concentration determination of embedded biological matter by scanning transmission electron microscopy
52. Monte Carlo calculations of elastic and inelastic electron scattering in biological and plastic materials
53. Contrast and resolution of scanning transmission electron microscope imaging modes
54. Correlation between structure and mass distribution of the nuclear pore complex and of distinct pore complex components
55. Biospecific labeling with undecagold: Visualization of the biotin-binding site on avidin
56. Actin labeling with undecagold
57. Imaging of RNA-protein interactions in splicing complexes with dark-field STEM
58. Quantitation of molecular densities by cryo-electron microscopy. Determination of the radial density distribution of Tobacco Mosaic Virus
59. Mass determination and estimation of subunit stoichiometry of the bacterial hook-basal body flagellar complex of Salmonella typhimurium by scanning transmission electron microscopy
60. Large nuclear ribonucleoprotein particles of specific RNA polymerase II transcripts
61. Evaluating atomic models of F-actin with an undecagold-tagged phalloidin derivative
62. The distribution of mass in heteropolymer intermediate filaments assembled in vitro. STEM analysis of vimentin/desmin and bovine epidermal keratin
63. Radial distributions of density within macromolecular complexes determined from dark-field electron micrographs
64. Water distributions of hydrated biological specimens by valence electron energy loss spectroscopy
65. Quantitative water mapping of cryosectioned cells by electron energy-loss spectroscopy
66. A molecular chaperone from a thermophilic archaebacterium is related to the eukariotic protein t-complex polypeptide-l
67. Visualization of protein-nucleic acid interactions involved in the in vitro assembly of the Escherichia coli 50S ribosomal subunit
68. The thermosome of Thermoplasma acidophilum and its relationship to the eukaryotic chaperonin TRiC
69. Biological Scanning Transmission Electron Microscopy
70. Mass mapping with the scanning transmission electron microscope
71. Scanning transmission electron microscopy at high resolution
72. Observation of an undecagold cluster compound in the scanning transmission electron microscope
73. Biological scanning transmission electron microscopy
74. Unshadowed myosin molecules: STEM mass-maps of myosin heads
75. The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins
76. A photometric procedure for weight determination of submicroscopic particles quantitative electron microscopy