Visualization of cell microtubules in their native state

Biology of the Cell

Volumn 99, Issue 1, 2007, Pages 45-53

  Bouchet Marquis, Cédric ^a   Zuber, Benoît ^a   Glynn, Anne Marie ^b   Eltsov, Mikhail ^a   Grabenbauer, Markus ^b   Goldie, Kenneth N ^b   Thomas, Daniel ^c   Frangakis, Achilleas S ^b   Dubochet, Jacques ^a   Chrétien, Denis ^c  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c UNIVERSITÉ DE RENNES 1   (France)

Author keywords

Cryo electron microscopy of vitreous sections (CEMOVIS); Cryo electron tomography; Luminal material; Microtubule; Tomography of vitreous sections (TOVIS)

Indexed keywords

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL POLARITY; CELL ULTRASTRUCTURE; CHO CELL; CRYOELECTRON MICROSCOPY; CYTOARCHITECTURE; FEMALE; HIPPOCAMPUS; IN VITRO STUDY; MICROFILAMENT; MICROSCOPIC ANATOMY; MICROTUBULE; MICROTUBULE ASSEMBLY; NONHUMAN; PRIORITY JOURNAL; RAT; THREE DIMENSIONAL IMAGING; TOMOGRAPHY; VITREOUS BODY;

ANIMALS; CELL POLARITY; CHO CELLS; CRICETINAE; CRICETULUS; CRYOELECTRON MICROSCOPY; HIPPOCAMPUS; MICROTUBULES; NEURONS; RATS;

CRICETULUS GRISEUS; RATTUS;

EID: 33846247396     PISSN: 02484900     EISSN: None     Source Type: Journal    
DOI: 10.1042/BC20060081     Document Type: Article

References (43)

1. Al-Amoudi, A., Chang, J.J., Leforestier, A., McDowall, A., Salamin, L.M., Norlen, L.P., Richter, K., Blanc, N.S., Studer, D. and Dubochet, J. (2004) Cryo-electron microscopy of vitreous sections. EMBO J. 23, 3583-3588
2. Al-Amoudi, A., Dubochet, J. and Norlen, L. (2005a) Nanostructure of the epidermal extracellular space as observed by cryo-electron microscopy of vitreous sections of human skin. J. Invest. Dermatol. 124, 764-777
3. Al-Amoudi, A., Studer, D. and Dubochet, J. (2005b) Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy. J. Struct. Biol. 150, 109-121
4. Bass, P.W., Deitch, J.S., Black, M.M. and Banker, G.A. (1988) Polarity orientation of microtubules in hippocampal neurons: uniformity in the axon and nonuniformity in the dendrite. Proc. Natl. Acad. Sci. U.S.A. 85, 8335-8339
5. Bouchet-Marquis, C., Dubochet, J. and Fakan, S. (2006) Cryoelectron microscopy of vitrified sections: a new challenge for the analysis of functional nuclear architecture. Histochem. Cell Biol. 125, 43-51
6. Burton, P.R. (1984) Luminal material in microtubules of frog olfactory axons: structure and distribution. J. Cell Biol. 99, 520-528
7. Chrétien, D. and Wade, R.H. (1991) New data on the microtubule surface lattice. Biol. Cell 71, 161-174
8. Chrétien, D., Fuller, S.D. and Karsenti, E. (1995) Structure of growing microtubule ends: two-dimensional sheets close into tubes at variable rates. J. Cell Biol. 129, 1311-1328
9. Chrétien, D., Kenney, J.M., Fuller, S.D. and Wade, R.H. (1996) Determination of microtubule polarity by cryo-electron microscopy. Structure 4, 1031-1040
10. Chrétien, D., Flyvbjerg, H. and Fuller, S.D. (1998) Limited flexibility of the inter-protofilament bonds in microtubules assembled from pure tubulin. Eur. Biophys. J. 27, 490-500
11. Dallai, R. and Afzelius, B.A. (1990) Microtubular diversity in insect spermatozoa: results obtained with a new fixative. J. Struct. Biol. 103, 164-179
12. Dubochet, J., Adrian, M., Chang, J.J., Homo, J.C., Lepault, J., McDowall, A.W. and Schultz, P. (1988) Cryo-electron microscopy of vitrified specimens. Q. Rev. Biophys. 21, 129-228
13. Frangakis, A.S., Bohm, J., Forster, F., Nickell, S., Nicastro, D., Typke, D., Hegerl, R. and Baumeister, W. (2002) Identification of macromolecular complexes in cryoelectron tomograms of phantom cells. Proc. Natl. Acad. Sci. U.S.A. 99, 14153-14158
14. Garvalov, B.K., Zuber, B., Bouchet-Marquis, C., Kudryashev, M., Gruska, M., Beck, M., Leis, A., Frischknecht, F., Bradke, F., Baumeister, W., Oubochet, J. and Cyrklaff, M. (2006) Luminal particles within cellular microtubules. J. Cell Biol. 174, 759-765
15. Hsieh, C.E., Leith, A., Mannella, C.A., Frank, J. and Marko, M. (2006) Towards high-resolution three-dimensional imaging of native mammalian tissue: electron tomography of frozen-hydrated rat liver sections. J. Struct. Biol. 153, 1-13
16. Hyams, J.S. and Lloyd, C.W. (1994) Microtubules. Wiley-Liss, New York
17. Li, H., DeRosier, D.J., Nicholson, W.V., Nogales, E. and Downing, K.H. (2002) Microtubule structure at 8 Å resolution. Structure 10, 1317-1328
18. Lowe, J., Li, H., Downing, K.H. and Nogales, E. (2001) Refined structure of αβ-tubulin at 3.5 Å resolution. J. Mol. Biol. 313, 1045-1057
19. Maiato, H., Sampaio, P. and Sunkel, C.E. (2004) Microtubule-associated proteins and their essential roles during mitosis. Int. Rev. Cytol. 241, 53-153
20. Mandelkow, E.M. and Mandelkow, E. (1985) Unstained microtubules studied by cryo-electron microscopy. Substructure, supertwist and disassembly. J. Mol. Biol. 181, 123-135
21. Matias, V.R. and Beveridge, T.J. (2006) Native cell wall organization shown by cryo-electron microscopy confirms the existence of a periplasmic space in Staphylococcus aureus. J. Bacteriol. 188, 1011-1021
22. McIntosh, J.R. and Euteneuer, U. (1984) Tubulin hooks as probes for microtubule polarity: an analysis of the method and an evaluation of data on microtubule polarity in the mitotic spindle. J. Cell Biol. 98, 525-533
23. Muller-Reichert, T., Chrétien, D., Severin, F. and Hyman, A.A. (1998) Structural changes at microtubule ends accompanying GTP hydrolysis: information from a slowly hydrolyzable analogue of GTP, guanylyl (α,β)methylenediphosphonate. Proc. Natl. Acad. Sci. U.S.A. 95, 3661-3666
24. Nickell, S., Kofler, C., Leis, A.P. and Baumeister, W. (2006) A visual approach to proteomics. Nat. Rev. Mol. Cell Biol. 7, 225-230
25. Nogales, E. and Grigorieff, N. (2001) Molecular Machines: putting the pieces together. J. Cell Biol. 152, F1-10
26. Nogales, E., Wolf, S.G. and Downing, K.H. (1998) Structure of the αβ-stubulin dimer by electron crystallography. Nature 391, 199-203
27. Orlova, E.V. and Saibil, H.R. (2004) Structure determination of macromolecular assemblies by single-particle analysis of cryo-electron micrographs. Curr. Qpin. Struct. Biol. 14, 584-590
28. Orren, D.K., Petersen, L.N. and Bohr, V.A. (1995) A UV-responsive G2 checkpoint in rodent cells. Mol. Cell. Biol. 15, 3722-3730
29. Rossmann, M.G., Morals, M.C., Leiman, P.G. and Zhang, W. (2005) Combining X-ray crystallography and electron microscopy. Structure 13, 355-362
30. Sartori Blanc, N., Studer, D., Ruhl, K. and Dubochet, J. (1998) Electron beam-induced changes in vitreous sections of biological samples. J. Microsc. 192, 194-201
31. Sosa, H. and Chrétien, D. (1998) Relationship between moire patterns, tubulin shape, and microtubule polarity. Cell Motil. Cytoskeleton 40, 38-43
32. Sosa, H., Dias, D.P., Hoenger, A., Whittaker, M., Wilson-Kubalek, E., Sablin, E., Fletterick, R.J., Vale, R.D. and Milligan, R.A. (1997) A model for the microtubule-Ncd motor protein complex obtained by cryo-electron microscopy and image analysis. Cell 90, 217-224
33. Stoppini, L., Buchs, P.A. and Muller, D. (1991) A simple method for organotypic cultures of nervous tissue. J. Neurosci. Methods 37, 173-182
34. Taylor, K.A. and Glaeser, R.M. (1976) Electron microscopy of frozen hydrated biological specimens. J. Ultrastruct. Res. 55, 448-456
35. Tilney, L.G., Bryan, J., Bush, D.J., Fujiwara, K., Mooseker, M.S., Murphy, D.B. and Snyder, D.H. (1973) Microtubules: evidence for 13 protofilaments. J. Cell Biol. 59, 267-275
36. Wade, R.H. and Chrétien, D. (1993) Cryoelectron microscopy of microtubules. J. Struct. Biol. 110, 1-27
37. Wade, R.H., Chrétien, D. and Job, D. (1990) Characterization of microtubule protofilament numbers. How does the surface lattice accommodate? J. Mol. Biol. 212, 775-786
38. Whitfield, J.F. (2004) The neuronal primary cilium - an extrasynaptic signaling device. Cell Signal. 16, 763-767
39. Xu, Z. and Afzelius, B.A. (1988a) Early changes in the substructure of the marginal bundle in human blood platelets responding to adenosine diphosphate. J. Ultrastruct. Mol. Struct. Res. 99, 254-260
40. Xu, Z. and Afzelius, B.A. (1988b) The substructure of marginal bundles in human blood platelets. J. Ultrastruct. Mol. Struct. Res. 99, 244-253
41. Zheng, Q.S., Braunfeld, M.B., Sedat, J.W. and Agard, D.A. (2004) An improved strategy for automated electron microscopic tomography. J. Struct. Biol. 147, 91-101
42. Zuber, B., Nikonenko, I., Klauser, P., Muller, D. and Dubochet, J. (2005) The mammalian central nervous synaptic cleft contains a high density of periodically organized complexes. Proc. Natl. Acad. Sci. U.S.A. 102, 19192-19197
43. Zuber, B., Haenni, M., Ribeiro, T., Minnig, K., Lopes, F., Moreillon, P. and Dubochet, J. (2006) Granular layer in the periplasmic space of Gram-positive bacteria and fine structure of Enterococcus gallinarum and Streptococcus gordonii septum revealed by cryo-electron microscopy of vitreous sections. J. Bacteriol. 188, 6652-6660