Polyethylenimine PEI F25-LMW allows the long-term storage of frozen complexes as fully active reagents in siRNA-mediated gene targeting and DNA delivery

European Journal of Pharmaceutics and Biopharmaceutics

Volumn 70, Issue 1, 2008, Pages 29-41

  Höbel, Sabrina ^a   Prinz, Robert ^a   Malek, Anastasia ^a   Urban Klein, Beata ^a   Sitterberg, Johannes ^b   Bakowsky, Udo ^b   Czubayko, Frank ^a   Aigner, Achim ^a  

^a Fachbereich Medizin der Universitat Marburg   (Germany)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

Frozen complexes; Nucleic acid delivery; PEI; Polyethylenimine; RNAi; siRNAs

Indexed keywords

BUFFER; DNA; GENE THERAPY AGENT; LOW MOLECULAR WEIGHT POLYETHYLENEIMINE F25; POLYETHYLENEIMINE DERIVATIVE; REAGENT; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ARTICLE; ATOMIC FORCE MICROSCOPY; COMPLEX FORMATION; CONTROLLED STUDY; CYTOTOXICITY; DIAGNOSTIC VALUE; DRUG FORMULATION; DRUG MANUFACTURE; GENE SILENCING; GENE TARGETING; GENE THERAPY; HUMAN; HUMAN CELL; IN VIVO STUDY; INCUBATION TIME; IONIC STRENGTH; MOLECULAR STABILITY; MOLECULAR WEIGHT; NUCLEIC ACID ANALYSIS; PH MEASUREMENT; PHYSICAL CHEMISTRY; REPRODUCIBILITY; RNA INTERFERENCE; STORAGE; ZETA POTENTIAL;

BUFFERS; CELL LINE, TUMOR; DNA; FREEZING; HUMANS; HYDROGEN-ION CONCENTRATION; INDICATORS AND REAGENTS; MICROSCOPY, ATOMIC FORCE; MOLECULAR WEIGHT; OSMOLAR CONCENTRATION; POLYETHYLENEIMINE; RNA INTERFERENCE; RNA STABILITY; RNA, SMALL INTERFERING; SERUM; TIME FACTORS; TRANSFECTION;

EID: 50149105616     PISSN: 09396411     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejpb.2008.03.014     Document Type: Article

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