Functional dissection of siRNA sequence by systematic DNA substitution: Modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect

Nucleic Acids Research

Volumn 36, Issue 7, 2008, Pages 2136-2151

  Ui Tei, Kumiko ^a   Naito, Yuki ^a   Zenno, Shuhei ^a,c   Nishi, Kenji ^a   Yamato, Kenji ^b   Takahashi, Fumitaka ^a   Juni, Aya ^a   Saigo, Kaoru ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^c MAEBASHI INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ARGONAUTE 2 PROTEIN; DNA; GENE PRODUCT; SMALL INTERFERING RNA; TRANS ACTIVATING RESPONSE RNA BINDING PROTEIN 2; UNCLASSIFIED DRUG; DEOXYRIBONUCLEOTIDE; MESSENGER RNA; OCTAMER TRANSCRIPTION FACTOR 4; RIBONUCLEOTIDE; RNA BINDING PROTEIN;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ANIMAL CELL; ARTICLE; CHIMERA; CONTROLLED STUDY; DNA MODIFICATION; DNA RNA HYBRIDIZATION; DNA STRAND; GENE SILENCING; GENE TARGETING; HUMAN; HUMAN CELL; MAMMALIAN GENETICS; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; PROTEIN TARGETING; RNA SEQUENCE; SEQUENCE HOMOLOGY; ANIMAL; AT RICH SEQUENCE; CELL DIFFERENTIATION; CELL LINE; CHEMISTRY; CYTOLOGY; DRUG ANTAGONISM; EMBRYONIC STEM CELL; GENETIC TRANSFECTION; GENOMICS; HAMSTER; METABOLISM; RNA INTERFERENCE;

MAMMALIA;

ANIMALS; AT RICH SEQUENCE; CELL DIFFERENTIATION; CELL LINE; CRICETINAE; DEOXYRIBONUCLEOTIDES; DNA; EMBRYONIC STEM CELLS; GENOMICS; HUMANS; OCTAMER TRANSCRIPTION FACTOR-3; RIBONUCLEOTIDES; RNA INTERFERENCE; RNA, MESSENGER; RNA, SMALL INTERFERING; RNA-BINDING PROTEINS; TRANSFECTION;

EID: 42449091989     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkn042     Document Type: Article

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