R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway

Science

Volumn 301, Issue 5641, 2003, Pages 1921-1925

  Liu, Qinghua ^a   Rand, Tim A ^a   Kalidas, Savitha ^b   Du, Fenghe ^a   Kim, Hyun Eui ^a   Smith, Dean P ^b   Wang, Xiaodong ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEGRADATION; ENZYMES; STOICHIOMETRY;

EFFECTOR STEPS;

RNA;

CAENORHABDITIS ELEGANS PROTEIN; DICER 2 PROTEIN; INSECT PROTEIN; MESSENGER RNA; R2D2 PROTEIN; RDE 4 PROTEIN; RNA; UNCLASSIFIED DRUG;

ENZYME; RNA;

ANIMAL CELL; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; DROSOPHILA; EMBRYO; ENZYME ACTIVITY; ENZYME SUBUNIT; INSECT CELL; NONHUMAN; PRIORITY JOURNAL; RNA DEGRADATION; RNA PROCESSING; RNA SEQUENCE; SEQUENCE HOMOLOGY; STOICHIOMETRY;

AMINO ACID SEQUENCE; ANIMALS; BIOTINYLATION; CAENORHABDITIS ELEGANS; CAENORHABDITIS ELEGANS PROTEINS; CELL LINE; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; ENDORIBONUCLEASES; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; PRECIPITATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; RIBONUCLEASE III; RNA HELICASES; RNA INTERFERENCE; RNA, DOUBLE-STRANDED; RNA, MESSENGER; RNA, SMALL INTERFERING; RNA-BINDING PROTEINS; RNA-INDUCED SILENCING COMPLEX;

ANIMALIA; ARACHNIDA; CAENORHABDITIS; CAENORHABDITIS ELEGANS; HEXAPODA; INSECTA;

EID: 0141868916     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1088710     Document Type: Article

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18. We thank G. Hannon, E. Bernstein, J. Abrams, and L Ford (Ambion) for useful reagents; H. Shu for mass spectrometric analysis; J. Morse for embryo injections; and J. Goldstein, K. Lynch, J. Rutter, X. Jiang, L. Wang, and P. Mishra for critical reading of the manuscript. This work was supported by a high-risk grant from UT Southwestern Medical Center and by grants from the American Heart Association (EI0040029N) and NIH (DC02539). T.A.R. is in the medical scientist training program. Q.L. is a GlaxoSmithKline Fellow of the Damon Runyon Cancer Research Foundation.