MicroRNA-Directed Cleavage of HOXB8 mRNA

Science

Volumn 304, Issue 5670, 2004, Pages 594-596

  Yekta, Soraya ^a,b   Shih, I Hung ^a   Bartel, David P ^a,b  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENES; GENETIC ENGINEERING; PROTEINS;

PROTEIN-CODING GENES; TRANSLATIONAL REPRESSION;

RNA;

MICRORNA;

BIOLOGY; RNA;

ANIMAL CELL; ARTICLE; CELL CULTURE; COMPLEMENTARITY DETERMINING REGION; CONTROLLED STUDY; DOWN REGULATION; EMBRYO; GENE EXPRESSION; HOMEOBOX; HOXA7 GENE; HOXB8 GENE; HOXC8 GENE; HOXD8 GENE; METAZOON; MOUSE; NONHUMAN; POSTTRANSCRIPTIONAL GENE SILENCING; PRIORITY JOURNAL; RNA CLEAVAGE; RNA GENE; RNA TRANSLATION;

3' UNTRANSLATED REGIONS; ANIMALS; BASE SEQUENCE; DOWN-REGULATION; GENES, HOMEOBOX; GENES, REPORTER; HELA CELLS; HOMEODOMAIN PROTEINS; HUMANS; MICE; MICRORNAS; MOLECULAR SEQUENCE DATA; NEOPLASM PROTEINS; RNA, MESSENGER; SEQUENCE ALIGNMENT; TRANSCRIPTION FACTORS; TRANSFECTION;

ANIMALIA; MAMMALIA; METAZOA; VERTEBRATA;

EID: 2142654329     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1097434     Document Type: Article

References (26)

1. M. Lagos-Quintana, R. Rauhut, J. Meyer, A. Borkhardt, T. Tuschl, RNA 9, 175 (2003).
2. L. P. Lim, M. E. Glasner, S. Yekta, C. B. Burge, D. P. Bartel, Science 299, 1540 (2003).
3. D. P. Bartel, Cell 116, 281 (2004).
4. R. Krumlauf, Cell 78, 191 (1994).
5. C. Llave, Z. Xie, K. D. Kasschau, J. C. Carrington, Science 297, 2053 (2002).
6. G. Tang, B. J. Reinhart, D. P. Bartel, P. D. Zamore, Genes Dev. 17, 49 (2003).
7. K. D. Kasschau et al., Dev. Cell 4, 205 (2003).
8. Y. Zeng, E. J. Wagner, B. R. Cullen, Mol. Cell 9, 1327 (2002).
9. G. Hutvágner, P. D. Zamore. Science 297, 2056 (2002); published online 1 August 2002; 10.1126/science.1073827.
10. Materials and methods are available as supporting material on Science Online.
11. B. P. Lewis, I. Shih, M. W. Jones-Rhoades, D. P. Bartel, C. B. Burge, Cell 115, 787 (2003).
12. P. H. Olsen, V. Ambros, Dev. Biol. 216, 671 (1999).
13. K. Seggerson, L. Tang, E. G. Moss, Dev. Biol. 243, 215 (2002).
14. M. J. Aukerman, H. Sakai, Plant Cell 15, 2730 (2003).
15. X. Chen, Science, in press; published online 31 July 2003; 10.1126/science.1088060.
16. J. G. Doench, C. P. Peterson, P. A. Sharp, Genes Dev. 17, 438 (2003).
17. Y. Zeng, R. Yi, B. R. Cullen, Proc. Natl. Acad. Sci. U.S.A. 100, 9779 (2003).
18. C. E. Nelson et al., Development 122, 1449 (1996).
19. T. Brend, J. Gilthorpe, D. Summerbell, P. W. Rigby, Development 130, 2717 (2003).
20. M. Kmita, D. Duboule, Science 301, 331 (2003).
21. L. P. Lim et al., Genes Dev. 17, 991 (2003).
22. A. A. Aravin et al., Dev. Cell 5, 337 (2003).
23. A. Stark, J. Brennecke, R. B. Russell, S. M. Cohen, PLoS Biol. 1, E60 (2003).
24. A. L. Jackson et al., Nat. Biotechnol. 21, 635 (2003).
25. J. G. Doench, P. A. Sharp, Genes Dev. 18, 504 (2004).
26. We thank W. Johnston for plasmid construction, J. Doench and P. Sharp for sharing ribonuclease-protection protocols and reagents, H. Lodish and D. Page for sharing facilities, and A. Chess, and C. Tabin for comments on this manuscript. Supported by a Cancer Research Institute Fellowship (I.S.) and a grant from NIH.