Mis-translation of a Computationally Designed Protein Yields an Exceptionally Stable Homodimer: Implications for Protein Engineering and Evolution

Journal of Molecular Biology

Volumn 362, Issue 5, 2006, Pages 1004-1024

  Dantas, Gautam ^a   Watters, Alexander L ^b   Lunde, Bradley M ^b   Eletr, Ziad M ^c   Isern, Nancy G ^d   Roseman, Toby ^b   Lipfert, Jan ^e   Doniach, Sebastian ^e   Tompa, Martin ^b   Kuhlman, Brian ^c   Stoddard, Barry L ^f   Varani, Gabriele ^a,b   Baker, David ^a,b  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

^c UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^d PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^e STANFORD UNIVERSITY   (United States)

^f Fred Hutchinson Cancer Research Center   (United States)

Author keywords

mistranslation; NMR structure; protein engineering; protein sub fragments; protein fold evolution

Indexed keywords

CELL PROTEIN; DISULFIDE; GLOBULAR PROTEIN; HOMODIMER; PROTEIN; TOP 7 PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANALYTICAL ERROR; ARTICLE; BINDING SITE; BIOLOGY; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; COVALENT BOND; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GENETIC SELECTION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STABILITY; PROTEIN STRUCTURE; PROTEIN VARIANT; RIBOSOME; SOLUTION AND SOLUBILITY; STATISTICAL ANALYSIS; TRANSLATION REGULATION; ULTRACENTRIFUGATION; X RAY CRYSTALLOGRAPHY;

ESCHERICHIA COLI;

EID: 33748474370     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2006.07.092     Document Type: Article

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