Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump

Science

Volumn 300, Issue 5621, 2003, Pages 976-980

  Yu, Edward W ^a   McDermott, Gerry ^b   Zgurskaya, Helen I ^a,c   Nikaido, Hiroshi ^a   Koshland Jr , Daniel E ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^c UNIVERSITY OF OKLAHOMA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CHEMOTHERAPY; HYDROPHOBICITY; ONCOLOGY; X RAY CRYSTALLOGRAPHY;

MULTIDRUG EFFLUX PUMPS;

DRUG PRODUCTS;

LIGAND;

ADMINISTRATION; DRUG DEVELOPMENT; PUMP;

ARTICLE; BACTERIAL INFECTION; CANCER CHEMOTHERAPY; CHEMICAL INTERACTION; DRUG BINDING; DRUG STRUCTURE; ESCHERICHIA COLI; HYDROPHOBICITY; LIGAND BINDING; MOLECULAR STABILITY; PRIORITY JOURNAL; X RAY CRYSTALLOGRAPHY;

ANTI-INFECTIVE AGENTS; ANTI-INFECTIVE AGENTS, LOCAL; BINDING SITES; CARRIER PROTEINS; CELL MEMBRANE; CHEMISTRY, PHYSICAL; CIPROFLOXACIN; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DEQUALINIUM; ELECTROSTATICS; ESCHERICHIA COLI PROTEINS; ETHIDIUM; HYDROGEN BONDING; HYDROPHOBICITY; LIGANDS; MEMBRANE PROTEINS; MODELS, MOLECULAR; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RHODAMINES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0038670226     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1083137     Document Type: Article

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23. We thank G. Meigs and H. Petras for technical support at the Advanced Light Source. Initial crystal screens were performed at the Stanford Synchrotron Radiation Laboratory. We also thank T. Alber and Y. Koshland for proofreading the manuscript. Supported by NIH grants AI 09644 (to H.N.) and an NIH postdoctoral fellowship (to E.W.Y.). Coordinates have been deposited with the Protein Data Bank. Accession codes for the unliganded, AcrB-R6G, AcrB-Et, AcrB-Dq, and AcrB-Cip structures are 1OY6, 1OY8, 1OY9, 1OYD, and 1OYE, respectively.