Cell biology: Protein synthesis upon acute nutrient restriction relies on proteasome function

Science

Volumn 310, Issue 5756, 2005, Pages 1960-1963

  Vabulas, Ramunas M ^a   Hartl, F Ulrich ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIODEGRADATION; BIOSYNTHESIS; CELLS;

CELL BIOLOGY; PROTEIN SYNTHESIS; TRANSLATION;

PROTEINS;

AMINO ACID; POLYPEPTIDE; PROTEASOME; AZETIDINE 2 CARBOXYLIC ACID; GREEN FLUORESCENT PROTEIN; PROTEIN KINASE; UBIQUITIN;

BIOLOGY;

ARTICLE; CELL SURVIVAL; MAMMAL CELL; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN SYNTHESIS; PROTEIN TRANSPORT; CELL LINE; DRUG ANTAGONISM; GENETICS; HELA CELL; HUMAN; METABOLISM; PHYSIOLOGY;

MAMMALIA;

AMINO ACIDS; AZETIDINECARBOXYLIC ACID; CELL LINE; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; PROTEASOME ENDOPEPTIDASE COMPLEX; PROTEIN BIOSYNTHESIS; PROTEIN KINASES; UBIQUITIN;

EID: 29344464782     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1121925     Document Type: Article

References (28)

1. G. E. Mortimore, A. R. Poso, Annu. Rev. Nutr. 7, 539 (1987).
2. A. Kuma et al., Nature 432, 1032 (2004).
3. N. Mizushima, A. Yamamoto, M. Matsui, T. Yoshimori, Y. Ohsumi, Mol. Biol. Cell 15, 1101 (2004).
4. S. H. Lecker, V. Solomon, W. E. Mitch, A. L. Goldberg, J. Nutr. 129, 227S (1999).
5. A. Hershko, A. Ciechanover, Annu. Rev. Biochem. 67, 425 (1998).
6. A. Varshavsky, Trends Biochem. Sci. 30, 283 (2005).
7. U. Schubert et al., Nature 404, 770 (2000).
8. E. S. Johnson, B. Bartel, W. Seufert, A. Varshavsky, EMBO J. 11, 497 (1992).
9. N. P. Dantuma, K. Lindsten, R. Glas, M. Jellne, M. G. Masucci, Nat. Biotecnnol. 18, 538 (2000).
10. Addition of 5 mM CHX together with radioactive tracer amino acid immediately blocked biosynthesis of polypeptides as evidenced by SDS-polyacrylamide gel electrophoresis (PAGE) analysis or from the lack of TCA-precipitable radioactivity.
11. R. M. Vabulas and F. U. Haitl, data not shown.
12. N. W. Tietz, Ed., Clinical Guide to Laboratory Tests (W. B. Saunders Company, Philadelphia, PA, 1995).
13. Materials and methods are available as supporting material on Science Online.
14. A. F. Kisselev, A. L. Goldberg, Chem. Biol. 8, 739 (2001).
15. T. Yoshimori, A. Yamamoto, Y. Moriyama, M. Futai, Y. Tashiro, J. Biol. Chem. 266, 17707 (1991).
16. I. Mellman, R. Fuchs, A. Helenius, Annu. Rev. Biochem. 55, 663 (1986).
17. V. Botbol, O. A. Scornik, J. Biol. Chem. 264, 13504 (1989).
18. K. Sekine, H. Fujii, F. Abe, K. Nishikawa, Int. J. Cancer 94, 485 (2001).
19. P. O. Seglen, P. B. Gordon, Proc. Natl. Acad Sci. U.S.A. 79, 1889 (1982).
20. P. O. Seglen, P. B. Gordon, H. Hoyvik, Biomed. Biochim. Acta 45, 1647 (1986).
21. S. A. Wek, S. Zhu, R. C. Wek, Mol. Cell. Biol. 15, 4497 (1995).
22. H. P. Harding et al., Mol. Cell 6, 1099 (2000).
23. A. Ciechanover, D. Finley, A. Varshavsky, Cell 37, 57 (1984).
24. K. L. Rock et al., Cell 78, 761 (1994).
25. A. J. McClellan, M. D. Scott, J. Frydman, Cell 121, 739 (2005).
26. C. Esser, S. Alberti, J. Hohfeld, Biochim. Biophys. Acta 1695, 171 (2004).
27. G. C. Tumer, A. Varshavsky, Science 289, 2117 (2000).
28. We thank K. Mann and W. Straßhofer for performing the amino acid analyses; J. M. Barral, P. Breuer, H.-C. Chang, N. Tzvetkov, and J. C. Young for stimulating discussion; and J. M. Barral and S. Broadley for critical reading of the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft (SFB 596).