Chemical protein synthesis methods in drug discovery

Current Opinion in Drug Discovery and Development

Volumn 4, Issue 2, 2001, Pages 205-214

  Kochendoerfer, G G ^a  

^a Gryphon Therapeutics   (United States)

Author keywords

Chemoselective ligation; FRET; Glycoprotein; Membrane protein; Native chemical ligation; Performance enhanced protein; Protein synthesis

Indexed keywords

CHEMOKINE RECEPTOR CCR5; GLYCOPROTEIN; MEMBRANE PROTEIN; PHOSPHOPROTEIN; RECEPTOR BLOCKING AGENT;

BIOSENSOR; CARBOXY TERMINAL SEQUENCE; CRYSTALLOGRAPHY; DRUG RESEARCH; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN SYNTHESIS; REACTION ANALYSIS; REVIEW;

ANIMALS; CHEMICAL ENGINEERING; HUMANS; PROTEINS; RECOMBINANT PROTEINS; TECHNOLOGY, PHARMACEUTICAL;

EID: 0035074833     PISSN: 13676733     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (63)

1. Synthesis of proteins by native chemical ligation
2. Modulation of reactivity in native chemical ligation through the use of thiol additives
3. Protein synthesis by native chemical ligation: Expanded scope by straightforward methodology
4. Native chemical ligation of polypeptides
5. Protein synthesis by chemical ligation of unprotected peptides in aqueous solution
6. Chemical protein synthesis
7. Chemical protein synthesis
8. Synthesis of native proteins by chemical ligation
9. A 'traceless' Staudinger ligation for the chemoselective synthesis of amide bonds
10. Staudinger ligation: A peptide from a thioester and azide
11. High-yielding Staudinger ligation of a phosphinothioester and azide to form a peptide
12. Solid-phase synthesis of peptide C-terminal thioesters by Fmoc/t-Bu chemistry
13. Fmoc-based synthesis of peptide-α-thioesters: Application to the total chemical synthesis of a glycoprotein by native chemical ligation
14. An alkanesulfonamide 'safety-catch' linker for solid-phase synthesis
15. Peptide thioester preparation by Fmoc solid-phase peptide synthesis for use in native chemical ligation
16. Expressed protein ligation: A general method for protein engineering
17. Semisynthesis of cytotoxic proteins using a modified protein splicing element
18. Expressed protein ligation, a novel method for studying protein-protein interactions in transcription
19. Chemical ligation of folded recombinant proteins: Segmental isotopic labeling of domains for NMR studies
20. Peptide ligation and its application to protein engineering
21. Semisynthesis of hyperphosphorylated type I TGFβ receptor: Addressing the mechanism of kinase activation
22. Intein-mediated synthesis of proteins containing carbohydrates and other molecular probes
23. New directions in glycoprotein engineering
24. Synthesis of peptides and proteins without cysteine residues by native chemical ligation combined with desulfurization
25. Conformationally assisted protein ligation using C-terminal thioester peptides
26. Native chemical ligation using removable Nα-(1-phenyl-2-mercaptoethyl) auxiliaries
27. Challenges for protein chemical synthesis in the 21st century: Bridging genomics and proteomics
28. Chemical ligation of cysteine-containing peptides: Synthesis of a 22 kDa tethered dimer of HIV-1 protease
29. Total chemical synthesis of a unique transcription factor-related protein: cMyc-Max
30. Chemical protein synthesis by solid-phase ligation of unprotected peptide segments
31. Do transmembrane protein superfolds exist?
32. Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms
33. Total chemical synthesis of the integral membrane protein influenza A virus M2: Role of its C-terminal domain in tetramer assembly
34. Enzymatic activity of a synthetic 99 residue protein corresponding to the putative HIV-1 protease
35. Conserved folding in retroviral proteases: Crystal structure of a synthetic HIV-1 protease
36. Structure of complex of synthetic HIV-1 protease with a substrate-based inhibitor at 2.3 Å resolution
37. Structure at 2.5 Å resolution of chemically synthesized human immunodeficiency virus type 1 protease complexed with a hydroxyethylene-based inhibitor
38. X-ray crystallographic structure of a complex between a synthetic protease of human immunodeficiency virus 1 and a substrate-based hydroxyethylamine inhibitor
39. Determining the 3D structure of HIV-1 protease
40. Probing intermolecular backbone H-bonding in serine proteinase-protein inhibitor complexes
41. Advances in direct methods for protein crystallography
42. Accessibility of selenomethionine proteins by total chemical synthesis: Structural studies of human herpesvirus-8 MIP-II
43. Comparison of the structure of vMIP-II with eotaxin-1, RANTES, and MCP-3 suggests a unique mechanism for CCR3 activation
44. Synthesis of multi-domain proteins using expressed protein ligation: Strategies for segmental isotopic labeling of internal regions
45. Selective 'in synthesis' labelling of peptides by fluorochromes
46. A highly efficient method for site-specific modification of unprotected peptides after chemical synthesis
47. Synthesis and evaluation of fluorescent chemokines labeled at the amino terminal
48. Time-resolved fluorescence probes for membrane protein chemical ligation
49. Luminescence resonance energy transfer
50. Rare earth cryptates and homogeneous fluoroimmunoassays with human sera
51. Probing molecular interactions with homogeneous techniques based on rare earth cryptates and fluorescence energy transfer
52. Design, total chemical synthesis, and binding properties of a [Leu-91-N1-methyl-7-azaTrp]Ras-binding domain of c-Raf-1
53. A sensitive fluorescence monitor for the detection of activated Ras: Total chemical synthesis of site specifically labeled Ras-binding domain of c-Raf1 immobilized on a surface
54. Generation of a dual-labeled fluorescence biosensor for Crk-II phosphorylation using solid-phase expressed protein ligation
55. Biosynthetic phage display: A novel protein engineering tool combining chemical and genetic diversity
56. Preparation of functionally active cell-permeable peptides by single-step ligation of two peptide modules
57. Dissecting G protein-coupled receptor signaling pathways with membrane-permeable blocking peptides. Endogenous 5-HT(2C) receptors in choroid plexus epithelial cells
58. Extension of recombinant human RANTES by the retention of the initiating methionine produces a potent antagonist
59. Potent inhibition of HIV-1 infectivity in macrophages and lymphocytes by a novel CCR5 antagonist
60. Total chemical synthesis and high-resolution crystal structure of the potent anti-HIV protein AOP-RANTES
61. Aminooxypentane-RANTES induces CCR5 internalization but inhibits recycling: A novel inhibitory mechanism of HIV infectivity
62. Highly potent RANTES analogues either prevent CCR5-using human immunodeficiency virus type 1 infection in vivo or rapidly select for CXCR4-using variants
63. Thiol-reactive, luminescent europium chelates: Luminescent probes for resonance energy transfer distance measurements in biomolecules