Production and recovery of recombinant protease inhibitor α1-antitrypsin

Enzyme and Microbial Technology

Volumn 29, Issue 10, 2001, Pages 611-620

  Tamer, I Melih ^a   Chisti, Yusuf ^b  

^a UNIVERSITY OF WATERLOO   (Canada)

^b MASSEY UNIVERSITY   (New Zealand)

Author keywords

1 antitrypsin; Cell disruption; Protease inhibitor; Recombinant Saccharomyces cerevisiae

Indexed keywords

AMMONIUM COMPOUNDS; BIOMASS; CELL CULTURE; FRACTIONATION; MICROFILTRATION;

BEAD MILLING;

ENZYME INHIBITION;

ALPHA 1 ANTITRYPSIN; ALUMINUM CHLORIDE; AMMONIUM SULFATE; PROTEINASE INHIBITOR;

ARTICLE; BIOMASS; CELL FREE SYSTEM; CELL SEPARATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME DEGRADATION; ENZYME SYNTHESIS; FLOCCULATION; FUNGUS CULTURE; FUNGUS GROWTH; HUMAN; MICROFILTRATION; NONHUMAN; PH; PRODUCTIVITY; SACCHAROMYCES CEREVISIAE; TEMPERATURE SENSITIVITY;

EID: 0035814361     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(01)00444-6     Document Type: Article

References (32)

1. 1-antitrypsin deficiency
2. 1-antitrypsin variants expressed in Escherichia coli
3. A rapid, and sensitive method for the quantitation of microgram quantities of protein utilising the principle of dye-binding
4. 1-antitrypsin cDNA in the yeast Saccharomyces cerevisiae
5. Automated fed-batch fermentation with feed-back controls based on dissolved oxygen (DO) and pH for production of DNA vaccines
6. A novel feeding strategy for enhanced plasmid stability, and protein production in recombinant yeast fed-batch fermentation
7. Strategies in downstream processing
8. Disruption of microbial cells for intracellular products
9. 1-antitrypsin in Saccharomyces cerevisiae using galactose as a gratuitous inducer
10. A robust fed-batch feeding strategy for optimal parameter estimation for baker's yeast production
11. Culture of Saccharomyces cerevisiae on hydrolysed waste cassava starch for production of baking-quality yeast
12. Controlled expression and purification of human immune interferon from high cell density fermentations of Saccharomyces cerevisiae
13. Disruption of a recombinant yeast for the release of β-galactosidase
14. 1-protease inhibitor by a unique procedure designed for industrial application
15. Recombinant protein production via fed-batch culture of the yeast Saccharomyces cerevisiae
16. 1-antitrypsin from Saccharomyces cerevisiae using inulinase signal sequence
17. 1-antitrypsin in Saccharomyces cerevisiae and methylotrophic yeasts
18. 1-antitrypsin secreted by recombinant yeast Saccharomyces diastaticus
19. 1-antitrypsin expressed as inclusion bodies in Escherichia coli: Characterization of aggregation
20. 1-antitrypsin toward urea denaturation and thermal deactivation
21. Microfiltration of recombinant yeast cells using a rotating disk dynamic filtration system
22. 1-antitrypsin
23. Broth conditioning and clarification
24. 1-proteinase inhibitor
25. 1-antitrypsin in transformed CHO cells
26. Synthesis in yeast of a functional oxidation resistant mutant of human α-1-antitrypsin
27. A rapid sensitive, and versatile assay for protein using Coomassie brilliant blue G250
28. 1-antitrypsin by plant-cell culture
29. Effects of sugar concentration on recombinant human α-1-antitrypsin production by genetically engineered rice cell
30. Isolation of a recombinant intracellular β-galactosidase by ammonium sulfate fractionation of cell homogenates
31. Plasmid stability in recombinant Saccharomyces cerevisiae