Simplified protein purification using engineered self-cleaving affinity tags

Journal of Chemical Technology and Biotechnology

Volumn 78, Issue 2-3, 2003, Pages 103-110

  Wood, David W ^a  

^a Princeton University   (United States)

Author keywords

Affinity tags; Bioprocessing; Bioseparations; Protein engineering; Protein precursors

Indexed keywords

BIOTECHNOLOGY; COSTS; GENES; PURIFICATION;

EXPRESSION SYSTEMS;

PROTEINS;

HYBRID PROTEIN; INTEIN; PROTEIN; PROTEIN PRECURSOR;

PROTEIN;

BIOENGINEERING; BIOPROCESS; BIOSEPARATION; BIOTECHNOLOGY; CLEAVED AMPLIFIED POLYMORPHIC SEQUENCE; CONFERENCE PAPER; COST BENEFIT ANALYSIS; COST EFFECTIVENESS ANALYSIS; EXPRESSED SEQUENCE TAG; GENE FUSION; GENETIC MANIPULATION; GENETIC SELECTION; MEDICAL GENETICS; NONHUMAN; OUTCOMES RESEARCH; PRODUCTIVITY; PROTEIN DEGRADATION; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN TARGETING; RELIABILITY; SEPARATION TECHNIQUE;

EID: 0037291488     PISSN: 02682575     EISSN: None     Source Type: Journal    
DOI: 10.1002/jctb.762     Document Type: Conference Paper

References (72)

1. Andersen DS and Krummen L, Recombinant protein expression for therapeutic applications. Curr Opin Biotechnol 13:117-123 (2002).
2. Ogden JE, Issues relating to the host expression system, in Understanding Biopharmaceuticals, Ed by Grindley J and Ogden JE, Interpharm Press, Denver, Colorado, USA. pp 35-95 (2000).
3. Yarmush ML and Jayaraman A, Advances in proteomic technologies. Annu Rev Biomed Eng 4:349-373 (2002).
4. Lee KH, Proteomics: A technology-driven and technology-limited discovery science. Trends Biotechnol 19:217-222 (2001).
5. Schellekens H and Ryff JC, 'Biogenerics': The off-patent biotech products. Trends Pharmacol Sci 23:119-121 (2002).
6. Freitag R and Horvath C, Chromatography in the downstream processing of biotechnological products. Adv Biochem Eng Biotechnol 53:17-59 (1996).
7. Fish NM and Lilly MD, The interactions between fermentation and protein recovery. Bio/Technology 2:623-627 (1984).
8. Cuatrecasas P, Wilchek M and Anfinsen CB, Selective enzyme purification by affinity chromatography. Proc Natl Acad Sci USA 61:636-643 (1968).
9. Wilchek M and Chaiken I, An overview of affinity chromatography. Methods Mol Biol 147:1-6 (2000).
10. Olson WP (ed), Separations Technology: Pharmaceutical and Biotechnology Applications, Interpharm Press, Inc, Buffalo Grove, IL (1995).
11. Campbell DH, Leuscher E and Lerman LS, The use of an immunologically specific adsorbent for purification of antibody. Proc Natl Acad Sci USA 37:575-578 (1951).
12. Axen R, Porath J and Ernback S, Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature 214:1302-1304 (1967).
13. Germino J and Bastia D, Rapid purification of a cloned gene product by genetic fusion and site-specific proteolysis. Proc Natl Acad Sci USA 81:46292-4696 (1984).
14. LaVallie ER and McCoy JM, Gene fusion expression systems in Escherichia coli. Curr Opin Biotechnol 6:501-506 (1995).
15. Riggs P, Expression and purification of recombinant proteins by fusion to maltose-binding protein. Mol Biotechnol 15:51-63 (2000).
16. Ford CF, Suominen I and Glatz CE, Fusion tails for the recovery and purification of recombinant proteins. Protein Expr Purif 2:95-107 (1991).
17. Hearn MT and Acosta D, Applications of novel affinity cassette methods: Use of peptide fusion handles for the purification of recombinant proteins. J Mol Recognit 14:323-369 (2001).
18. Kapust RB and Waugh DS, Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci 8:1668-1674 (1999).
19. Bach H, Mazor Y, Shaky S, Shoham-Lev A, Berdichevsky Y, Gutnick DL and Benhar I, Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies. J Mol Biol 312:79-93 (2001).
20. Lowe CR, Lowe AR and Gupta G, New developments in affinity chromatography with potential application in the production of biopharmaceuticals. J Biochem Biophys Methods 49:561-574 (2001).
21. Leong LE, The use of recombinant fusion proteases in the affinity purification of recombinant proteins. Mol Biotechnol 12:269-274 (1999).
22. Chase HA and Draeger NM, Affinity purification of proteins using expanded beds. J Chromatogr 597:129-145 (1992).
23. Clemmirtt RH and Chase HA, Facilitated downstream processing of a histidine-tagged protein from unclarified E coli homogenates using immobilized metal affinity expanded-bed adsorption. Biotechnol Bioeng 67:206-216 (2000).
24. Xu MQ, Paulus H and Chong S, Fusions to self-splicing inteins for protein purification. Methods Enzymol 326:376-418 (2000).
25. Hirata R, Ohsumk Y, Nakano A, Kawasaki H, Suzuki K and Anraku Y, Molecular structure of a gene, VMAi, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. J Biol Chem 265:6726-6733 (1990).
26. Perler FB, InBase: The Intein Database. Nucleic Acids Res 30:383-384 (2002).
27. Perler FB, Davis EO, Dean GE, Gimble FS, Jack WE, Neff N, Noren CJ, Thorner J and Belfort M, Protein splicing elements: Inteins and exteins - A definition of terms and recommended nomenclature. Nucleic Acids Res 22:1125-1127 (1994).
28. Davis EO, Jenner PJ, Brooks PC, Colston MJ and Sedgwick SG, Protein splicing in the maturation of M tuberculosis recA protein: A mechanism for tolerating a novel class of intervening sequence. Cell 71:201-210 (1992).
29. Perler FB, Comb DG, Jack WE, Moran LS, Qiang B, Kucera RB, Benner J, Slatko BE, Nwankwo DO, Hempstead SK, Carlow CKS and Jannasch H, Intervening sequences in an Archaea DNA polymerase gene. Proc Natl Acad Sci USA 89:5577-5581 (1992).
30. Gu HH, Xu J, Gallagher M and Dean GE, Peptide splicing in the vacuolar ATPase subunit A from Candida tropicalis. J Biol Chem 268:7372-7381 (1993).
31. Liu XQ and Hu Z, Identification and characterization of a cyanobacterial DnaX intein. FEBS Lett 408:311-314 (1997).
32. Wang S and Liu XQ, Identification of an unusual intein in chloroplast ClpP protease of Chlamydomonas eugametos. J Biol Chem 272:11869-11873 (1997).
33. Cooper AA and Stevens TH, Protein splicing: Excision of intervening sequences at the protein level. Bioessays 15:667-674 (1993).
34. Derbyshire V, Wood DW, Wu W, Dansereau JT, Dalgaard JZ and Belfort M, Genetic definition of a protein-splicing domain: Functional mini-inteins support structure predictions and a model for intein evolution. Proc Natl Acad Sci USA 94:11466-11471 (1997).
35. Davis EO, Thangaraj HS, Brooks PC and Colston MJ, Evidence of selection for protein introns in the recAs of pathogenic mycobacteria. Embo J 13:699-703 (1994).
36. Noren CJ, Wang J and Perler FB, Dissecting the chemistry of protein splicing and its applications. Angew Chem Int Ed Engl 39:450-466 (2000).
37. Southworth MW, Adam E, Panne D, Byer R, Kautz R and Perler FB, Control of protein splicing by intein fragment reassembly. Embo J 17:918-926 (1998).
38. Vogel G, A two-piece protein assembles itself. Science 281;763-764 (1998).
39. Cowburn D and Muir TW, Segmental isotopic labeling using expressed protein ligation. Methods Enzymol 339:41-54 (2001).
40. Mathys S, Evans TC, Chute IC, Wu H, Chong S, Benner J, Liu XQ and Xu MQ, Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: Facile production of protein building blocks for protein ligation. Gene 231:1-13 (1999).
41. Scott CP, Abel-Santos E, Wall M, Wahnon DC and Benkovic SJ, Production of cyclic peptides and proteins in vivo. Proc Natl Acad Sci USA 96:13638-13643 (1999).
42. Chong S, Shao Y, Paulus H, Benner J, Perler FB and Xu MQ, Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system. J Biol Chem 271:22159-22168 (1996).
43. Paulus H, Protein splicing and related forms of protein autoprocessing. Annu Rev Biochem 69:447-496 (2000).
44. Chong S, Mersha FB, Comb DG, Scott ME, Landry D, Vence LM, Perler FB, Benner J, Kucera RB, Hirvonen CA, Pelletier JJ, Paulus H and Xu MQ, Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 192:271-281 (1997).
45. Chong S, Montello GE, Zhang A, Cantor EJ, Liao W, Xu MQ and Benner J, Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucleic Acids Res 26:5109-5115 (1998).
46. Inoue K, Demel R, De Kruijff B and Keegstra K, The N-terminal portion of the preToc75 transit peptide interacts with membrane lipids and inhibits binding and import of precursor proteins into isolated chloroplasts. Eur J Biochem 268:4036-4043 (2001).
47. Hong S, Toyama M, Maret W and Murooka Y, High yield expression and single step purification of human thionein/metallothionein. Protein Expr Purif 21:243-250 (2001).
48. Cantor EJ and Chong S, Intein-mediated rapid purification of Cre recombinase. Protein Expr Purif 22:135-140 (2001).
49. Saiki K, Konishi K, Gomi T, Nishihara T and Yoshikawa M, Reconstitution and purification of cytolethal distending toxin of Actinobacillus actinomycetemcomitans. Microbiol Immunol 45:497-506 (2001).
50. Thomson CA and Ananthanarayanan VS, A method for expression and purification of soluble, active Hsp47, a collagen-specific molecular chaperone. Protein Expr Purif 23:8-13 (2001).
51. Gorbalenya AE, Non-canonical inteins. Nucleic Acids Res 26:1741-1748 (1998).
52. Pietrokovski S, Identification of a virus intein and a possible variation in the protein-splicing reaction. Curr Biol 8:R634-635 (1998).
53. Southworth MW, Benner J and Perler FB, An alternative protein splicing mechanism for inteins lacking an N-terminal nucleophile. Embo J 19:5019-5026 (2000).
54. Chong S, Williams KS, Wotkowicz C and Xu MQ, Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein. J Biol Chem 273:10567-10577 (1998).
55. Xu MQ and Perler FB, The mechanism of protein splicing and its modulation by mutation. Embo J 15:5146-5153 (1996).
56. Kawasaki M, Nogami S, Satow Y, Ohya Y and Ankaru Y, Identification of three core regions essential for protein splicing of the yeast Vma1 protozyme. A random mutagenesis study of the entire Vma1-derived endonuclease sequence. J Biol Chem 272:15668-15674 (1997).
57. Amitai G and Pietrokovski S, Fine-tuning an engineered intein. Nat Biotechnol 17:854-855 (1999).
58. Poland BW, Xu MQ and Quiocho FA, Structural insights into the protein splicing mechanism of PI-SceI. J Biol Chem 275:16408-16413 (2000).
59. Dalgaard JZ, Moser MJ, Hughey R and Mian IS, Statistical modeling, phylogenetic analysis and structure prediction of a protein splicing domain common to inteins and hedgehog proteins. J Comput Biol 4:193-214 (1997).
60. Duan X, Gimble FS and Quiocho FA, Crystal structure of PI-SceI, a homing endonuclease with protein splicing activity. Cell 89:555-564 (1997).
61. Ichiyanagi K, Ishino Y, Ariyoshi M, Komori K and Morikawa K, Crystal structure of an archaeal intein-encoded homing endonuclease Pi-PfuI. J Mol Biol 300:889-901 (2000).
62. Davis EO, Sedgwick SG and Colston MJ, Novel structure of the recA locus of Mycobacterium tuberculosis implies processing of the gene product. J Bacteriol 173:5653-5662 (1991).
63. Shingledecker K, Jiang SQ and Paulus H, Molecular dissection of the Mycobacterium tuberculosis RecA intein: Design of a minimal intein and of a trans-splicing system involving two intein fragments. Gene 207:187-195 (1998).
64. Wood DW, Wu W, Belfort G, Derbyshire V and Belfort M, A genetic system yields self-cleaving inteins for bioseparations. Nat Biotechnol 17:889-892 (1999).
65. Wood DW, Derbyshire V, Wu W, Chartrain M, Belfort M and Belfort G, Optimized single-step affinity purification with a self-cleaving intein applied to human acidic fibroblast growth factor. Biotechnol Prog 16:1055-1063 (2000).
66. Southworth MW, Amaya K, Evans TC, Xu MQ and Perler FB, Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. Biotechniques 27:110-114, 116, 118-120 (1999).
67. Zhang A, Gonzalez SM, Cantor EJ and Chong S, Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins. Gene 275:241-252 (2001).
68. Myscofski DM, Dutton EK, Cantor E, Zhang Z and Hruby DE, Cleavage and purification of intein fusion proteins using the Streptococcus gordonii spex system. Prep Biochem Biotechnol 31:275-290 (2001).
69. Evans TC, Jr, Benner J and Xu MQ, The in vitro ligation of bacterially expressed proteins using an intein from Methanobacterium thermoautotrophicum. J Biol Chem 274:3923-3926 (1999).
70. Ghosh I, Sun L and Xu MQ, Zinc inhibition of protein transplicing and identification of regions essential for splicing and association of a split intein*. J Biol Chem 276:24051-24058 (2001).
71. Mills KV and Paulus H, Reversible inhibition of protein splicing by zinc ion. J Biol Chem 276:10832-10838 (2001).
72. Daugelat S and Jacobs WR Jr, The Mycobacterium tuberculosis recA intein can be used in an ORFTRAP to select for open reading frames. Protein Sci 8:644-653 (1999).