Monitoring GFP-operon fusion protein expression during high cell density cultivation of Escherichia coli using an on-line optical sensor

Biotechnology and Bioengineering

Volumn 65, Issue 1, 1999, Pages 54-64

  Delisa, Matthew P ^a,b   Li, Jincai ^a,c   Rao, Govind ^a,c   Weigand, William A ^b   Bentley, William E ^a,b  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b UNIVERSITY OF MARYLAND   (United States)

^c UNIVERSITY OF MARYLAND BALTIMORE COUNTY   (United States)

Author keywords

Escherichia coli; Green fluorescent protein; High cell density cultivation; On line monitoring; Sensor

Indexed keywords

BIOMASS; BIOSYNTHESIS; CELL CULTURE; ENZYME KINETICS; ENZYMES; ESCHERICHIA COLI; FERMENTATION; GENES; GLUCOSE; GROWTH KINETICS; MATHEMATICAL MODELS; OPTICAL SENSORS;

CHLORAMPHENICOL ACETYLTRANSFERASE; ENZYMATIC ACTIVITY; GREEN FLUORESCENT PROTEIN; HIGH CELL DENSITY CULTIVATION; TRANSCRIPTION;

PROTEINS;

ARABINOSE; CHLORAMPHENICOL ACETYLTRANSFERASE; GLUCOSE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BIOMASS; CELL DENSITY; ESCHERICHIA COLI; FERMENTATION; NONHUMAN; OPERON; SENSOR;

ARABINOSE; BIOSENSING TECHNIQUES; BIOTECHNOLOGY; CHLORAMPHENICOL O-ACETYLTRANSFERASE; COLONY COUNT, MICROBIAL; CULTURE MEDIA; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; ONLINE SYSTEMS; OPERON; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI; FELIS CATUS; NEGIBACTERIA;

EID: 0033527396     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19991005)65:1<54::AID-BIT7>3.0.CO;2-R     Document Type: Article

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