Effect of construct design on MAPKAP kinase-2 activity, thermodynamic stability and ligand-binding affinity

Archives of Biochemistry and Biophysics

Volumn 449, Issue 1-2, 2006, Pages 47-56

  Kervinen, Jukka ^a   Ma, Hongchang ^a   Bayoumy, Shariff ^a   Schubert, Carsten ^a   Milligan, Cynthia ^a   Lewandowski, Frank ^a   Moriarty, Kevin ^a   DesJarlais, Renee L ^a   Ramachandren, Kannan ^a   Wang, Haiyun ^b   Harris, Crafford A ^b   Grasberger, Bruce ^a   Todd, Matthew ^a   Springer, Barry A ^a   Deckman, Ingrid ^a  

^a Development LLC   (United States)

^b JOHNSON AND JOHNSON PHARMACEUTICAL RESEARCH AND DEVELOPMENT   (United States)

Author keywords

Baculovirus; Equilibrium ligand binding assay; Inflammatory disease; MAP kinase; MAPKAPK2

Indexed keywords

4 (4 FLUOROPHENYL) 2 (4 HYDROXYPHENYL) 5 (4 PYRIDYL)IMIDAZOLE; 4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL)IMIDAZOLE; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE DERIVATIVE; K 252A; MAGNESIUM; MITOGEN ACTIVATED PROTEIN KINASE KINASE 2; MITOGEN ACTIVATED PROTEIN KINASE P38 INHIBITOR; NUCLEOTIDE; STAUROSPORINE; ISOENZYME; LIGAND; MAP KINASE ACTIVATED KINASE 2; MAP-KINASE-ACTIVATED KINASE 2; PROTEIN SERINE THREONINE KINASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BACULOVIRUS; BINDING AFFINITY; BINDING ASSAY; ENZYME ACTIVITY; IC 50; INSECT CELL; LIGAND BINDING; MUTANT; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; THERMODYNAMICS; THERMOSTABILITY; BINDING SITE; CHEMISTRY; ENZYME ACTIVATION; ENZYME STABILITY; PROTEIN BINDING; STRUCTURE ACTIVITY RELATION; TEMPERATURE;

INSECTA;

BINDING SITES; ENZYME ACTIVATION; ENZYME STABILITY; ISOENZYMES; LIGANDS; PROTEIN BINDING; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; TEMPERATURE; THERMODYNAMICS;

EID: 33745167708     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2006.03.018     Document Type: Article

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