FRET-based detection of different conformations of MK2

EMBO Reports

Volumn 2, Issue 8, 2001, Pages 703-708

  Neininger, Armin ^a   Thielemann, Heiko ^a   Gaestel, Matthias ^b  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; GREEN FLUORESCENT PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE KINASE; STRESS ACTIVATED PROTEIN KINASE;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL ACTIVATION; CELL NUCLEUS; CELL TRANSPORT; CYTOPLASM; EMBRYO; EMBRYO CELL; ENERGY TRANSFER; ENZYME ACTIVATION; ENZYME CONFORMATION; ENZYME PHOSPHORYLATION; FLUORESCENCE; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; KIDNEY TUMOR; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN VARIANT; TUMOR CELL;

ACTIVE TRANSPORT, CELL NUCLEUS; ANISOMYCIN; CELL LINE; ENERGY TRANSFER; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HUMANS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; MITOGEN-ACTIVATED PROTEIN KINASES; PHOSPHORYLATION; PROTEIN CONFORMATION; PROTEIN KINASES; PROTEIN SYNTHESIS INHIBITORS; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE;

EID: 0034859534     PISSN: 1469221X     EISSN: None     Source Type: Journal    
DOI: 10.1093/embo-reports/kve157     Document Type: Article

References (33)

1. Two co-existing mechanisms for nuclear import of MAP kinase: Passive diffusion of a monomer and active transport of a dimer
2. Nuclear export of the stress-activated protein kinase p38 mediated by its substrate MAPKAP kinase-2
3. Fluorescence resonance energy transfer and nucleic acids
4. Constitutive activation of mitogen-activated protein kinase-activated protein kinase 2 by mutation of phosphorylation sites and an A-helix motif
5. Leptomycin B-sensitive nuclear export of MAPKAP kinase 2 is regulated by phosphorylation
6. Anisomycin selectively desensitizes signalling components involved in stress kinase activation and fos and jun induction
7. MAPKAP kinase 2 phosphorylates serum response factor in vitro and in vivo
8. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer
9. LSP1 is the major substrate for mitogen-activated protein kinase-activated protein kinase 2 in human neutrophils
10. Phosphorylation of the MAP kinase ERK2 promotes its homodimerization and nuclear translocation
11. Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase
12. MAPKAP kinase 2 is essential for LPS-induced TNF-α biosynthesis
13. Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein
14. 2+ based on green fluorescent proteins and calmodulin
15. A fluorescent indicator for visualizing cAMP-induced phosphorylation in vivo
16. Serine/threonine kinases 3pK and MAPK-activated protein kinase 2 interact with the basic helix-loop-helix transcription factor E47 and repress its transcriptional activity
17. The p38 signal transduction pathway: Activation and function
18. Characterization of the proline-rich region of mouse MAPKAP kinase 2: Influence on catalytic properties and binding to the c-abl SH3 domain in vitro
19. MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway
20. 2+-dependent changes in the fluorescence emission of an indicator composed of two green fluorescent protein variants linked by a calmodulin-binding sequence. A new class of fluorescent indicators
21. A FRET-based sensor reveals large ATP hydrolysis-induced conformational changes and three distinct states of the molecular motor myosin
22. Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation of the small mammalian heat shock proteins
23. Swing of the lever arm of a myosin motor at the isomerization and phosphate-release steps
24. A conserved docking motif in MAP kinases common to substrates, activators and regulators
25. Participation of a stress-activated protein kinase cascade in the activation of tyrosine hydroxylase in chromaffin cells
26. The green fluorescent protein
27. Rapid caspase-3 activation during apoptosis revealed using fluorescence-resonance energy transfer
28. A conserved helix motif complements the protein kinase core
29. 5-lipoxygenase is phosphorylated by p38 kinase-dependent MAPKAP kinases
30. Leptomycin B is an inhibitor of nuclear export: Inhibition of nucleo-cytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) Rev protein and Rev-dependent mRNA
31. A genetically encoded, fluorescent indicator for cyclic AMP in living cells
32. Characterization of an autoinhibitory domain in human mitogen-activated protein kinase-activated protein kinase 2