A faster migrating variant masquerades as NICD when performing in vitro γ-secretase assays with bacterially expressed notch substrates

Biochemistry

Volumn 45, Issue 16, 2006, Pages 5351-5358

  Keller II, Preston C ^a   Tomita, Taisuke ^a,b   Hayashi, Ikuo ^b   Chandu, Dilip ^a   Weber, Jason D ^a   Cistola, David P ^a   Kopan, Raphael ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; BIOCHEMISTRY; BIOLOGICAL MEMBRANES; CELLS; ENZYMES; PROTEINS; SUBSTRATES;

BIOCHEMICAL PROPERTIES; INSECT CELLS; INTRAMEMBRANE PROTEOLYSIS; NOTCH SUBSTRATES;

ENZYME KINETICS;

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 2 HYDROXY 1 PROPANESULFONIC ACID; GAMMA SECRETASE; NOTCH RECEPTOR; PROTEINASE INHIBITOR; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; ARTIFACT; CARBOXY TERMINAL SEQUENCE; HUMAN; HUMAN CELL; IN VITRO STUDY; INSECT CELL; MAMMAL CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN;

AMYLOID PRECURSOR PROTEIN SECRETASES; ASPARTIC ENDOPEPTIDASES; CELL LINE; ENDOPEPTIDASES; GENE EXPRESSION REGULATION, BACTERIAL; HUMANS; HYDROGEN-ION CONCENTRATION; RECEPTORS, NOTCH; SUBSTRATE SPECIFICITY; TIME FACTORS;

ANIMALIA; INSECTA; MAMMALIA;

EID: 33645972587     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi052228a     Document Type: Article

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