Optimizing RNA interference for application in mammalian cells

Biochemical Journal

Volumn 380, Issue 3, 2004, Pages 593-603

  Medema, René H ^a  

^a NETHERLANDS CANCER INSTITUTE   (Netherlands)

Author keywords

Complementation analysis; Mitosis; Off target; Secondary effect; Small interfering RNA (siRNA); Synchronization

Indexed keywords

COST EFFECTIVENESS; GENES; GENETIC ENGINEERING; LABORATORIES; MUTAGENESIS; RNA;

CELL CYCLE; LIFE SCIENCES; MAMMALIAN CELLS; MUTATIONAL ANALYSIS;

CELLS;

LENTIVIRUS VECTOR; RETROVIRUS VECTOR; TRANSFER RNA;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; BASE PAIRING; CAENORHABDITIS ELEGANS; EXPRESSION VECTOR; GENE CONTROL; GENE EXPRESSION REGULATION; GENE SILENCING; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; MAMMAL CELL; MOLECULAR EVOLUTION; NONHUMAN; PLANT GENETICS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STABILITY; REVIEW; RNA DEGRADATION; RNA INTERFERENCE; RNA PROCESSING; TRANSLATION INITIATION;

ANIMALS; GENETIC TECHNIQUES; HUMANS; RNA INTERFERENCE;

CAENORHABDITIS; CAENORHABDITIS ELEGANS; LENTIVIRUS; MAMMALIA; UNIDENTIFIED RETROVIRUS; VECTORS; VERTEBRATA;

EID: 3042704068     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20040260     Document Type: Review

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