U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells

Nature Biotechnology

Volumn 20, Issue 5, 2002, Pages 497-500

  Miyagishi, Makoto ^a   Taira, Kazunari ^a,b  

^a UNIVERSITY OF TOKYO   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; GENES; PROTEINS; RNA;

VIRAL INFECTIONS;

BIOTECHNOLOGY;

DOUBLE STRANDED RNA; RECOMBINANT RNA; RNA; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; URIDINE;

ARTICLE; EXPRESSION VECTOR; GENE DISRUPTION; GENE EXPRESSION SYSTEM; GENE REPRESSION; GENE SILENCING; GENE TARGETING; GENETIC TRANSFECTION; HOST RESISTANCE; HUMAN; HUMAN CELL; MAMMAL CELL; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN SYNTHESIS INHIBITION; VIRAL GENE DELIVERY SYSTEM;

ANIMALS; BLOTTING, NORTHERN; BLOTTING, WESTERN; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE EXPRESSION REGULATION; GENE TRANSFER TECHNIQUES; GENES, REPORTER; GENETIC VECTORS; HELA CELLS; HUMANS; MICROSCOPY, FLUORESCENCE; MODELS, GENETIC; OLIGONUCLEOTIDES, ANTISENSE; RNA, SMALL INTERFERING; RNA, SMALL NUCLEAR; RNA, UNTRANSLATED; TRANSFECTION; URIDINE;

CAENORHABDITIS; CAENORHABDITIS ELEGANS; MAMMALIA; NEMATODA; PROTOZOA; VECTORS;

EID: 0036245628     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0502-497     Document Type: Article

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