메뉴 건너뛰기




Volumn 21, Issue 7, 2003, Pages 312-317

Antibodies in proteomics II: Screening, high-throughput characterization and downstream applications

Author keywords

[No Author keywords available]

Indexed keywords

BINDERS; CELLS; FLUORESCENCE; IMMUNOLOGY;

EID: 10744220060     PISSN: 01677799     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7799(03)00117-3     Document Type: Review
Times cited : (54)

References (64)
  • 1
    • 0026317443 scopus 로고
    • By-passing immunization. Human antibodies from V-gene libraries displayed on phage
    • Marks J.D., et al. By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222:1991;581-597.
    • (1991) J. Mol. Biol. , vol.222 , pp. 581-597
    • Marks, J.D.1
  • 2
    • 0034425742 scopus 로고    scopus 로고
    • Antibody arrays for high-throughput screening of antibody-antigen interactions
    • de Wildt R.M., et al. Antibody arrays for high-throughput screening of antibody-antigen interactions. Nat. Biotechnol. 18:2000;989-994.
    • (2000) Nat. Biotechnol. , vol.18 , pp. 989-994
    • De Wildt, R.M.1
  • 3
    • 18344385481 scopus 로고    scopus 로고
    • Autoantigen microarrays for multiplex characterization of autoantibody responses
    • Robinson W.H., et al. Autoantigen microarrays for multiplex characterization of autoantibody responses. Nat. Med. 8:2002;295-301.
    • (2002) Nat. Med. , vol.8 , pp. 295-301
    • Robinson, W.H.1
  • 4
    • 0035151047 scopus 로고    scopus 로고
    • Advances in protein microarray technology for protein expression and interaction profiling
    • Haab B.B. Advances in protein microarray technology for protein expression and interaction profiling. Curr. Opin. Drug Discov. Devel. 4:2001;116-123.
    • (2001) Curr. Opin. Drug Discov. Devel. , vol.4 , pp. 116-123
    • Haab, B.B.1
  • 5
    • 0035221240 scopus 로고    scopus 로고
    • Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions
    • RESEARCH0004
    • Haab B.B., et al. Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions. Genome Biol. 2001;2. RESEARCH0004.
    • (2001) Genome Biol. , pp. 2
    • Haab, B.B.1
  • 6
    • 12444317574 scopus 로고    scopus 로고
    • Antibodies in proteomics 1: Generating antibodies
    • Bradbury A. Antibodies in proteomics 1: generating antibodies. Trends Biotechnol. 2003;21.
    • (2003) Trends Biotechnol. , pp. 21
    • Bradbury, A.1
  • 7
    • 0035400341 scopus 로고    scopus 로고
    • Antibodies in haystacks: How selection strategy influences the outcome of selection from molecular diversity libraries
    • Lou J., et al. Antibodies in haystacks: how selection strategy influences the outcome of selection from molecular diversity libraries. J. Immunol. Methods. 253:2001;233-242.
    • (2001) J. Immunol. Methods , vol.253 , pp. 233-242
    • Lou, J.1
  • 8
    • 0026528230 scopus 로고
    • Miniantibodies: Use of amphipathic helices to produce functional, flexibly linked dimeric FV fragments with high avidity in Escherichia coli
    • Pack P., Pluckthun A. Miniantibodies: use of amphipathic helices to produce functional, flexibly linked dimeric FV fragments with high avidity in Escherichia coli. Biochemistry. 31:1992;1579-1584.
    • (1992) Biochemistry , vol.31 , pp. 1579-1584
    • Pack, P.1    Pluckthun, A.2
  • 9
    • 0022340978 scopus 로고
    • Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product
    • Evan G.I., et al. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5:1985;3610-3616.
    • (1985) Mol. Cell. Biol. , vol.5 , pp. 3610-3616
    • Evan, G.I.1
  • 10
    • 0026568735 scopus 로고
    • Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen
    • Hanke T., et al. Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J. Gen. Virol. 73:1992;653-660.
    • (1992) J. Gen. Virol. , vol.73 , pp. 653-660
    • Hanke, T.1
  • 11
    • 0030738617 scopus 로고    scopus 로고
    • New protein engineering approaches to multivalent and bispecific antibody fragments
    • Pluckthun A., Pack P. New protein engineering approaches to multivalent and bispecific antibody fragments. Immunotechnology. 3:1997;83-105.
    • (1997) Immunotechnology , vol.3 , pp. 83-105
    • Pluckthun, A.1    Pack, P.2
  • 12
    • 0028945817 scopus 로고
    • High-affinity antigen binding by chelating recombinant antibodies (CRAbs)
    • Neri D., et al. High-affinity antigen binding by chelating recombinant antibodies (CRAbs). J. Mol. Biol. 246:1995;367-373.
    • (1995) J. Mol. Biol. , vol.246 , pp. 367-373
    • Neri, D.1
  • 13
    • 0031032155 scopus 로고    scopus 로고
    • Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system
    • Krebber A., et al. Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. J. Immunol. Methods. 201:1997;35-55.
    • (1997) J. Immunol. Methods , vol.201 , pp. 35-55
    • Krebber, A.1
  • 14
    • 0035427605 scopus 로고    scopus 로고
    • High-throughput generation and engineering of recombinant human antibodies
    • Krebs B., et al. High-throughput generation and engineering of recombinant human antibodies. J. Immunol. Methods. 254:2001;67-84.
    • (2001) J. Immunol. Methods , vol.254 , pp. 67-84
    • Krebs, B.1
  • 15
    • 0033152010 scopus 로고    scopus 로고
    • PSKAP/S: An expression vector for the production of single-chain Fv alkaline phosphatase fusion proteins
    • Griep R.A., et al. pSKAP/S: an expression vector for the production of single-chain Fv alkaline phosphatase fusion proteins. Protein Expr. Purif. 16:1999;63-69.
    • (1999) Protein Expr. Purif. , vol.16 , pp. 63-69
    • Griep, R.A.1
  • 16
    • 0031039221 scopus 로고    scopus 로고
    • Targeting vectors for intracellular immunisation
    • Persic L., et al. Targeting vectors for intracellular immunisation. Gene. 187:1997;1-8.
    • (1997) Gene , vol.187 , pp. 1-8
    • Persic, L.1
  • 17
    • 0031038715 scopus 로고    scopus 로고
    • An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries
    • Persic L., et al. An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries. Gene. 187:1997;9-18.
    • (1997) Gene , vol.187 , pp. 9-18
    • Persic, L.1
  • 18
    • 0033676098 scopus 로고    scopus 로고
    • DNA cloning using in vitro site-specific recombination
    • Hartley J.L., et al. DNA cloning using in vitro site-specific recombination. Genome Res. 10:2000;1788-1795.
    • (2000) Genome Res. , vol.10 , pp. 1788-1795
    • Hartley, J.L.1
  • 19
    • 0035093709 scopus 로고    scopus 로고
    • Open-reading-frame sequence tags (OSTs) support the existence of at least 17,300 genes in C. elegans
    • Reboul J., et al. Open-reading-frame sequence tags (OSTs) support the existence of at least 17,300 genes in C. elegans. Nat. Genet. 27:2001;332-336.
    • (2001) Nat. Genet. , vol.27 , pp. 332-336
    • Reboul, J.1
  • 20
    • 0035929244 scopus 로고    scopus 로고
    • Using an in vivo phagemid system to identify non-compatible loxP sequences
    • Siegel R.W., et al. Using an in vivo phagemid system to identify non-compatible loxP sequences. FEBS Lett. 505:2001;467-473.
    • (2001) FEBS Lett. , vol.505 , pp. 467-473
    • Siegel, R.W.1
  • 21
    • 0032541483 scopus 로고    scopus 로고
    • Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination
    • Lee G., Saito I. Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination. Gene. 216:1998;55-65.
    • (1998) Gene , vol.216 , pp. 55-65
    • Lee, G.1    Saito, I.2
  • 22
    • 0023046599 scopus 로고
    • The role of the loxP spacer region in P1 site-specific recombination
    • Hoess R.H., et al. The role of the loxP spacer region in P1 site-specific recombination. Nucleic Acids Res. 14:1986;2287-2300.
    • (1986) Nucleic Acids Res. , vol.14 , pp. 2287-2300
    • Hoess, R.H.1
  • 23
    • 0033987925 scopus 로고    scopus 로고
    • Exploiting recombination in single bacteria to make large phage antibody libraries
    • Sblattero D., Bradbury A. Exploiting recombination in single bacteria to make large phage antibody libraries. Nat. Biotechnol. 18:2000;75-80.
    • (2000) Nat. Biotechnol. , vol.18 , pp. 75-80
    • Sblattero, D.1    Bradbury, A.2
  • 24
    • 0031822837 scopus 로고    scopus 로고
    • Tissue microarrays for high-throughput molecular profiling of tumor specimens
    • Kononen J., et al. Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat. Med. 4:1998;844-847.
    • (1998) Nat. Med. , vol.4 , pp. 844-847
    • Kononen, J.1
  • 25
    • 0033645558 scopus 로고    scopus 로고
    • An automated machine vision system for the histological grading of cervical intraepithelial neoplasia (CIN)
    • Keenan S.J., et al. An automated machine vision system for the histological grading of cervical intraepithelial neoplasia (CIN). J. Pathol. 192:2000;351-362.
    • (2000) J. Pathol. , vol.192 , pp. 351-362
    • Keenan, S.J.1
  • 26
    • 0036840104 scopus 로고    scopus 로고
    • Morphometry of dermal collagen orientation by Fourier analysis is superior to multi-observer assessment
    • van Zuijlen P.P., et al. Morphometry of dermal collagen orientation by Fourier analysis is superior to multi-observer assessment. J. Pathol. 198:2002;284-291.
    • (2002) J. Pathol. , vol.198 , pp. 284-291
    • Van Zuijlen, P.P.1
  • 27
    • 0035663101 scopus 로고    scopus 로고
    • The application of protein microarrays to serum diagnostics: Prostate cancer as a test case
    • Miller J.C., et al. The application of protein microarrays to serum diagnostics: prostate cancer as a test case. Dis. Markers. 17:2001;225-234.
    • (2001) Dis. Markers , vol.17 , pp. 225-234
    • Miller, J.C.1
  • 28
    • 0034622925 scopus 로고    scopus 로고
    • Printing proteins as microarrays for high-throughput function determination
    • MacBeath G., Schreiber S.L. Printing proteins as microarrays for high-throughput function determination. Science. 289:2000;1760-1763.
    • (2000) Science , vol.289 , pp. 1760-1763
    • MacBeath, G.1    Schreiber, S.L.2
  • 29
    • 0035848705 scopus 로고    scopus 로고
    • Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front
    • Paweletz C.P., et al. Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front. Oncogene. 20:2001;1981-1989.
    • (2001) Oncogene , vol.20 , pp. 1981-1989
    • Paweletz, C.P.1
  • 30
    • 0035887145 scopus 로고    scopus 로고
    • Profiling of cancer cells using protein microarrays: Discovery of novel radiation-regulated proteins
    • Sreekumar A., et al. Profiling of cancer cells using protein microarrays: discovery of novel radiation-regulated proteins. Cancer Res. 61:2001;7585-7593.
    • (2001) Cancer Res. , vol.61 , pp. 7585-7593
    • Sreekumar, A.1
  • 31
    • 18344374893 scopus 로고    scopus 로고
    • Multiplexed protein profiling on microarrays by rolling-circle amplification
    • Schweitzer B., et al. Multiplexed protein profiling on microarrays by rolling-circle amplification. Nat. Biotechnol. 20:2002;359-365.
    • (2002) Nat. Biotechnol. , vol.20 , pp. 359-365
    • Schweitzer, B.1
  • 32
    • 0036898579 scopus 로고    scopus 로고
    • Protein microarrays and proteomics
    • MacBeath G. Protein microarrays and proteomics. Nat. Genet. 32:(Suppl. 1):2002;526-532.
    • (2002) Nat. Genet. , vol.32 , Issue.SUPPL. 1 , pp. 526-532
    • MacBeath, G.1
  • 33
    • 18544397601 scopus 로고    scopus 로고
    • Directed evolution of high-affinity antibody mimics using mRNA display
    • Xu L., et al. Directed evolution of high-affinity antibody mimics using mRNA display. Chem. Biol. 9:2002;933-942.
    • (2002) Chem. Biol. , vol.9 , pp. 933-942
    • Xu, L.1
  • 34
    • 0035003057 scopus 로고    scopus 로고
    • Protein chips based on recombinant antibody fragments: A highly sensitive approach as detected by mass spectrometry
    • Borrebaeck C.A., et al. Protein chips based on recombinant antibody fragments: a highly sensitive approach as detected by mass spectrometry. BioTechniques. 30:2001;1126-1132.
    • (2001) BioTechniques , vol.30 , pp. 1126-1132
    • Borrebaeck, C.A.1
  • 36
    • 0027787769 scopus 로고
    • Intracellular expression of anti-p21ras single chain Fv fragments inhibits meiotic maturation of Xenopus oocytes
    • Biocca S., et al. Intracellular expression of anti-p21ras single chain Fv fragments inhibits meiotic maturation of Xenopus oocytes. Biochem. Biophys. Res. Commun. 197:1993;422-427.
    • (1993) Biochem. Biophys. Res. Commun. , vol.197 , pp. 422-427
    • Biocca, S.1
  • 37
    • 0029826880 scopus 로고    scopus 로고
    • Human recombinant antibody fragments neutralising human immunodeficiency virus type 1 reverse transcriptase provide an experimental basis for the structural classification of the DNA polymerase family
    • Gargano N., et al. Human recombinant antibody fragments neutralising human immunodeficiency virus type 1 reverse transcriptase provide an experimental basis for the structural classification of the DNA polymerase family. J. Virol. 70:1996;7706-7712.
    • (1996) J. Virol. , vol.70 , pp. 7706-7712
    • Gargano, N.1
  • 38
    • 0030776175 scopus 로고    scopus 로고
    • Inhibition of murine leukaemia virus retrotranscription by the intracellular expression of a phage-derived anti-reverse transcriptase antibody fragment
    • Gargano N., Cattaneo A. Inhibition of murine leukaemia virus retrotranscription by the intracellular expression of a phage-derived anti-reverse transcriptase antibody fragment. J. Gen. Virol. 78:1997;2591-2599.
    • (1997) J. Gen. Virol. , vol.78 , pp. 2591-2599
    • Gargano, N.1    Cattaneo, A.2
  • 39
    • 85031151400 scopus 로고    scopus 로고
    • The mode of action of the Y13-259 scFv fragment when expressed intracellularly in mammalian cells
    • in press
    • Cardinale, A. et al. The mode of action of the Y13-259 scFv fragment when expressed intracellularly in mammalian cells. FEBS Lett. (in press).
    • FEBS Lett.
    • Cardinale, A.1
  • 40
    • 0028914482 scopus 로고
    • Inhibition of HIV-1 Tat-mediated LTR transactivation and HIV-1 infection by anti-Tat single chain intrabodies
    • Mhashilkar A.M., et al. Inhibition of HIV-1 Tat-mediated LTR transactivation and HIV-1 infection by anti-Tat single chain intrabodies. EMBO J. 14:1995;1542-1551.
    • (1995) EMBO J. , vol.14 , pp. 1542-1551
    • Mhashilkar, A.M.1
  • 41
    • 0028955535 scopus 로고
    • Phenotypic knockout of the high-affinity human interleukin 2 receptor by intracellular single-chain antibodies against the α subunit of the receptor
    • Richardson J.H., et al. Phenotypic knockout of the high-affinity human interleukin 2 receptor by intracellular single-chain antibodies against the α subunit of the receptor. Proc. Natl. Acad. Sci. U. S. A. 92:1995;3137-3141.
    • (1995) Proc. Natl. Acad. Sci. U. S. A. , vol.92 , pp. 3137-3141
    • Richardson, J.H.1
  • 42
    • 0028024772 scopus 로고
    • Intracellular single chain Fv antibody inhibits Ras activity in T-cell antigen receptor stimulated Jurkat cells
    • Werge T.M., et al. Intracellular single chain Fv antibody inhibits Ras activity in T-cell antigen receptor stimulated Jurkat cells. FEBS Lett. 351:1994;393-396.
    • (1994) FEBS Lett. , vol.351 , pp. 393-396
    • Werge, T.M.1
  • 43
    • 0033952543 scopus 로고    scopus 로고
    • Diverting a protein from its cellular location by intracellular antibodies. The case of p21Ras
    • Lener M., et al. Diverting a protein from its cellular location by intracellular antibodies. The case of p21Ras. Eur. J. Biochem. 267:2000;1196-1205.
    • (2000) Eur. J. Biochem. , vol.267 , pp. 1196-1205
    • Lener, M.1
  • 44
    • 0027275371 scopus 로고
    • Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gp120 single-chain antibody
    • Marasco W.A., et al. Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gp120 single-chain antibody. Proc. Natl. Acad. Sci. U. S. A. 90:1993;7889-7893.
    • (1993) Proc. Natl. Acad. Sci. U. S. A. , vol.90 , pp. 7889-7893
    • Marasco, W.A.1
  • 45
    • 85047699270 scopus 로고    scopus 로고
    • Intrabody-mediated phenotypic knockout of major histocompatibility complex class I expression in human and monkey cell lines and in primary human keratinocytes
    • Mhashilkar A.M., et al. Intrabody-mediated phenotypic knockout of major histocompatibility complex class I expression in human and monkey cell lines and in primary human keratinocytes. Gene Ther. 9:2002;307-319.
    • (2002) Gene Ther. , vol.9 , pp. 307-319
    • Mhashilkar, A.M.1
  • 46
    • 85031157285 scopus 로고    scopus 로고
    • Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework
    • in press
    • Auf der Maur A. et al. Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework. J. Biol. Chem. (in press).
    • J. Biol. Chem.
    • Auf der Maur, A.1
  • 47
    • 0033499632 scopus 로고    scopus 로고
    • Extended half-life and elevated steady-state level of a single-chain Fv intrabody are critical for specific intracellular retargeting of its antigen, caspase-7
    • Zhu Q., et al. Extended half-life and elevated steady-state level of a single-chain Fv intrabody are critical for specific intracellular retargeting of its antigen, caspase-7. J. Immunol. Methods. 231:1999;207-222.
    • (1999) J. Immunol. Methods , vol.231 , pp. 207-222
    • Zhu, Q.1
  • 48
    • 0033520343 scopus 로고    scopus 로고
    • Intrabody construction and expression. I. The critical role of VL domain stability
    • Ohage E., Steipe B. Intrabody construction and expression. I. The critical role of VL domain stability. J. Mol. Biol. 291:1999;1119-1128.
    • (1999) J. Mol. Biol. , vol.291 , pp. 1119-1128
    • Ohage, E.1    Steipe, B.2
  • 49
    • 0036299049 scopus 로고    scopus 로고
    • The intracellular antibody capture technology (IACT): Towards a consensus sequence for intracellular antibodies
    • Visintin M., et al. The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies. J. Mol. Biol. 317:2002;73-83.
    • (2002) J. Mol. Biol. , vol.317 , pp. 73-83
    • Visintin, M.1
  • 50
    • 0033520452 scopus 로고    scopus 로고
    • Intrabody construction and expression. II. A synthetic catalytic Fv fragment
    • Ohage E.C., et al. Intrabody construction and expression. II. A synthetic catalytic Fv fragment. J. Mol. Biol. 291:1999;1129-1134.
    • (1999) J. Mol. Biol. , vol.291 , pp. 1129-1134
    • Ohage, E.C.1
  • 51
    • 0028111743 scopus 로고
    • Sequence statistics reliably predict stabilizing mutations in a protein domain
    • Steipe B., et al. Sequence statistics reliably predict stabilizing mutations in a protein domain. J. Mol. Biol. 240:1994;188-192.
    • (1994) J. Mol. Biol. , vol.240 , pp. 188-192
    • Steipe, B.1
  • 52
    • 0032718607 scopus 로고    scopus 로고
    • Intrabody construction and expression III: Engineering hyperstable V(H) domains
    • Wirtz P., Steipe B. Intrabody construction and expression III: engineering hyperstable V(H) domains. Protein Sci. 8:1999;2245-2250.
    • (1999) Protein Sci. , vol.8 , pp. 2245-2250
    • Wirtz, P.1    Steipe, B.2
  • 53
    • 0035823143 scopus 로고    scopus 로고
    • Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies
    • Bach H., et al. Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies. J. Mol. Biol. 312:2001;79-93.
    • (2001) J. Mol. Biol. , vol.312 , pp. 79-93
    • Bach, H.1
  • 54
    • 0033865190 scopus 로고    scopus 로고
    • Engineered protein scaffolds for molecular recognition
    • Skerra A. Engineered protein scaffolds for molecular recognition. J. Mol. Recognit. 13:2000;167-187.
    • (2000) J. Mol. Recognit. , vol.13 , pp. 167-187
    • Skerra, A.1
  • 55
    • 0028982245 scopus 로고
    • A combinatorial library of an α-helical bacterial receptor domain
    • Nord K., et al. A combinatorial library of an α-helical bacterial receptor domain. Protein Eng. 8:1995;601-608.
    • (1995) Protein Eng. , vol.8 , pp. 601-608
    • Nord, K.1
  • 56
    • 0029876415 scopus 로고    scopus 로고
    • Genetic selection of peptide aptamers that recognize and inhibit cyclin-dependent kinase 2
    • Colas P., et al. Genetic selection of peptide aptamers that recognize and inhibit cyclin-dependent kinase 2. Nature. 380:1996;548-550.
    • (1996) Nature , vol.380 , pp. 548-550
    • Colas, P.1
  • 57
    • 0034610373 scopus 로고    scopus 로고
    • Targeted modification and transportation of cellular proteins
    • Colas P., et al. Targeted modification and transportation of cellular proteins. Proc. Natl. Acad. Sci. U. S. A. 97:2000;13720-13725.
    • (2000) Proc. Natl. Acad. Sci. U. S. A. , vol.97 , pp. 13720-13725
    • Colas, P.1
  • 58
    • 0032564350 scopus 로고    scopus 로고
    • An artificial cell-cycle inhibitor isolated from a combinatorial library
    • Cohen B.A., et al. An artificial cell-cycle inhibitor isolated from a combinatorial library. Proc. Natl. Acad. Sci. U. S. A. 95:1998;14272-14277.
    • (1998) Proc. Natl. Acad. Sci. U. S. A. , vol.95 , pp. 14272-14277
    • Cohen, B.A.1
  • 59
    • 0037173610 scopus 로고    scopus 로고
    • Modulation of HIV-1 replication by RNA interference
    • Jacque J.M., et al. Modulation of HIV-1 replication by RNA interference. Nature. 418:2002;435-438.
    • (2002) Nature , vol.418 , pp. 435-438
    • Jacque, J.M.1
  • 60
    • 0036245628 scopus 로고    scopus 로고
    • U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells
    • Miyagishi M., Taira K. U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nat. Biotechnol. 20:2002;497-500.
    • (2002) Nat. Biotechnol. , vol.20 , pp. 497-500
    • Miyagishi, M.1    Taira, K.2
  • 61
    • 0037117550 scopus 로고    scopus 로고
    • A DNA vector-based RNAi technology to suppress gene expression in mammalian cells
    • Sui G., et al. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc. Natl. Acad. Sci. U. S. A. 99:2002;5515-5520.
    • (2002) Proc. Natl. Acad. Sci. U. S. A. , vol.99 , pp. 5515-5520
    • Sui, G.1
  • 62
    • 0035694237 scopus 로고    scopus 로고
    • Identification of essential genes in cultured mammalian cells using small interfering RNAs
    • Harborth J., et al. Identification of essential genes in cultured mammalian cells using small interfering RNAs. J. Cell Sci. 114:2001;4557-4565.
    • (2001) J. Cell Sci. , vol.114 , pp. 4557-4565
    • Harborth, J.1
  • 63
    • 0035942736 scopus 로고    scopus 로고
    • Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
    • Elbashir S.M., et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411:2001;494-498.
    • (2001) Nature , vol.411 , pp. 494-498
    • Elbashir, S.M.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.