-
1
-
-
0026317443
-
By-passing immunization. Human antibodies from V-gene libraries displayed on phage
-
Marks J.D., et al. By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222:1991;581-597.
-
(1991)
J. Mol. Biol.
, vol.222
, pp. 581-597
-
-
Marks, J.D.1
-
2
-
-
0034425742
-
Antibody arrays for high-throughput screening of antibody-antigen interactions
-
de Wildt R.M., et al. Antibody arrays for high-throughput screening of antibody-antigen interactions. Nat. Biotechnol. 18:2000;989-994.
-
(2000)
Nat. Biotechnol.
, vol.18
, pp. 989-994
-
-
De Wildt, R.M.1
-
3
-
-
18344385481
-
Autoantigen microarrays for multiplex characterization of autoantibody responses
-
Robinson W.H., et al. Autoantigen microarrays for multiplex characterization of autoantibody responses. Nat. Med. 8:2002;295-301.
-
(2002)
Nat. Med.
, vol.8
, pp. 295-301
-
-
Robinson, W.H.1
-
4
-
-
0035151047
-
Advances in protein microarray technology for protein expression and interaction profiling
-
Haab B.B. Advances in protein microarray technology for protein expression and interaction profiling. Curr. Opin. Drug Discov. Devel. 4:2001;116-123.
-
(2001)
Curr. Opin. Drug Discov. Devel.
, vol.4
, pp. 116-123
-
-
Haab, B.B.1
-
5
-
-
0035221240
-
Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions
-
RESEARCH0004
-
Haab B.B., et al. Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions. Genome Biol. 2001;2. RESEARCH0004.
-
(2001)
Genome Biol.
, pp. 2
-
-
Haab, B.B.1
-
6
-
-
12444317574
-
Antibodies in proteomics 1: Generating antibodies
-
Bradbury A. Antibodies in proteomics 1: generating antibodies. Trends Biotechnol. 2003;21.
-
(2003)
Trends Biotechnol.
, pp. 21
-
-
Bradbury, A.1
-
7
-
-
0035400341
-
Antibodies in haystacks: How selection strategy influences the outcome of selection from molecular diversity libraries
-
Lou J., et al. Antibodies in haystacks: how selection strategy influences the outcome of selection from molecular diversity libraries. J. Immunol. Methods. 253:2001;233-242.
-
(2001)
J. Immunol. Methods
, vol.253
, pp. 233-242
-
-
Lou, J.1
-
8
-
-
0026528230
-
Miniantibodies: Use of amphipathic helices to produce functional, flexibly linked dimeric FV fragments with high avidity in Escherichia coli
-
Pack P., Pluckthun A. Miniantibodies: use of amphipathic helices to produce functional, flexibly linked dimeric FV fragments with high avidity in Escherichia coli. Biochemistry. 31:1992;1579-1584.
-
(1992)
Biochemistry
, vol.31
, pp. 1579-1584
-
-
Pack, P.1
Pluckthun, A.2
-
9
-
-
0022340978
-
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product
-
Evan G.I., et al. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5:1985;3610-3616.
-
(1985)
Mol. Cell. Biol.
, vol.5
, pp. 3610-3616
-
-
Evan, G.I.1
-
10
-
-
0026568735
-
Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen
-
Hanke T., et al. Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J. Gen. Virol. 73:1992;653-660.
-
(1992)
J. Gen. Virol.
, vol.73
, pp. 653-660
-
-
Hanke, T.1
-
11
-
-
0030738617
-
New protein engineering approaches to multivalent and bispecific antibody fragments
-
Pluckthun A., Pack P. New protein engineering approaches to multivalent and bispecific antibody fragments. Immunotechnology. 3:1997;83-105.
-
(1997)
Immunotechnology
, vol.3
, pp. 83-105
-
-
Pluckthun, A.1
Pack, P.2
-
12
-
-
0028945817
-
High-affinity antigen binding by chelating recombinant antibodies (CRAbs)
-
Neri D., et al. High-affinity antigen binding by chelating recombinant antibodies (CRAbs). J. Mol. Biol. 246:1995;367-373.
-
(1995)
J. Mol. Biol.
, vol.246
, pp. 367-373
-
-
Neri, D.1
-
13
-
-
0031032155
-
Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system
-
Krebber A., et al. Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. J. Immunol. Methods. 201:1997;35-55.
-
(1997)
J. Immunol. Methods
, vol.201
, pp. 35-55
-
-
Krebber, A.1
-
14
-
-
0035427605
-
High-throughput generation and engineering of recombinant human antibodies
-
Krebs B., et al. High-throughput generation and engineering of recombinant human antibodies. J. Immunol. Methods. 254:2001;67-84.
-
(2001)
J. Immunol. Methods
, vol.254
, pp. 67-84
-
-
Krebs, B.1
-
15
-
-
0033152010
-
PSKAP/S: An expression vector for the production of single-chain Fv alkaline phosphatase fusion proteins
-
Griep R.A., et al. pSKAP/S: an expression vector for the production of single-chain Fv alkaline phosphatase fusion proteins. Protein Expr. Purif. 16:1999;63-69.
-
(1999)
Protein Expr. Purif.
, vol.16
, pp. 63-69
-
-
Griep, R.A.1
-
16
-
-
0031039221
-
Targeting vectors for intracellular immunisation
-
Persic L., et al. Targeting vectors for intracellular immunisation. Gene. 187:1997;1-8.
-
(1997)
Gene
, vol.187
, pp. 1-8
-
-
Persic, L.1
-
17
-
-
0031038715
-
An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries
-
Persic L., et al. An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries. Gene. 187:1997;9-18.
-
(1997)
Gene
, vol.187
, pp. 9-18
-
-
Persic, L.1
-
18
-
-
0033676098
-
DNA cloning using in vitro site-specific recombination
-
Hartley J.L., et al. DNA cloning using in vitro site-specific recombination. Genome Res. 10:2000;1788-1795.
-
(2000)
Genome Res.
, vol.10
, pp. 1788-1795
-
-
Hartley, J.L.1
-
19
-
-
0035093709
-
Open-reading-frame sequence tags (OSTs) support the existence of at least 17,300 genes in C. elegans
-
Reboul J., et al. Open-reading-frame sequence tags (OSTs) support the existence of at least 17,300 genes in C. elegans. Nat. Genet. 27:2001;332-336.
-
(2001)
Nat. Genet.
, vol.27
, pp. 332-336
-
-
Reboul, J.1
-
20
-
-
0035929244
-
Using an in vivo phagemid system to identify non-compatible loxP sequences
-
Siegel R.W., et al. Using an in vivo phagemid system to identify non-compatible loxP sequences. FEBS Lett. 505:2001;467-473.
-
(2001)
FEBS Lett.
, vol.505
, pp. 467-473
-
-
Siegel, R.W.1
-
21
-
-
0032541483
-
Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination
-
Lee G., Saito I. Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination. Gene. 216:1998;55-65.
-
(1998)
Gene
, vol.216
, pp. 55-65
-
-
Lee, G.1
Saito, I.2
-
22
-
-
0023046599
-
The role of the loxP spacer region in P1 site-specific recombination
-
Hoess R.H., et al. The role of the loxP spacer region in P1 site-specific recombination. Nucleic Acids Res. 14:1986;2287-2300.
-
(1986)
Nucleic Acids Res.
, vol.14
, pp. 2287-2300
-
-
Hoess, R.H.1
-
23
-
-
0033987925
-
Exploiting recombination in single bacteria to make large phage antibody libraries
-
Sblattero D., Bradbury A. Exploiting recombination in single bacteria to make large phage antibody libraries. Nat. Biotechnol. 18:2000;75-80.
-
(2000)
Nat. Biotechnol.
, vol.18
, pp. 75-80
-
-
Sblattero, D.1
Bradbury, A.2
-
24
-
-
0031822837
-
Tissue microarrays for high-throughput molecular profiling of tumor specimens
-
Kononen J., et al. Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat. Med. 4:1998;844-847.
-
(1998)
Nat. Med.
, vol.4
, pp. 844-847
-
-
Kononen, J.1
-
25
-
-
0033645558
-
An automated machine vision system for the histological grading of cervical intraepithelial neoplasia (CIN)
-
Keenan S.J., et al. An automated machine vision system for the histological grading of cervical intraepithelial neoplasia (CIN). J. Pathol. 192:2000;351-362.
-
(2000)
J. Pathol.
, vol.192
, pp. 351-362
-
-
Keenan, S.J.1
-
26
-
-
0036840104
-
Morphometry of dermal collagen orientation by Fourier analysis is superior to multi-observer assessment
-
van Zuijlen P.P., et al. Morphometry of dermal collagen orientation by Fourier analysis is superior to multi-observer assessment. J. Pathol. 198:2002;284-291.
-
(2002)
J. Pathol.
, vol.198
, pp. 284-291
-
-
Van Zuijlen, P.P.1
-
27
-
-
0035663101
-
The application of protein microarrays to serum diagnostics: Prostate cancer as a test case
-
Miller J.C., et al. The application of protein microarrays to serum diagnostics: prostate cancer as a test case. Dis. Markers. 17:2001;225-234.
-
(2001)
Dis. Markers
, vol.17
, pp. 225-234
-
-
Miller, J.C.1
-
28
-
-
0034622925
-
Printing proteins as microarrays for high-throughput function determination
-
MacBeath G., Schreiber S.L. Printing proteins as microarrays for high-throughput function determination. Science. 289:2000;1760-1763.
-
(2000)
Science
, vol.289
, pp. 1760-1763
-
-
MacBeath, G.1
Schreiber, S.L.2
-
29
-
-
0035848705
-
Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front
-
Paweletz C.P., et al. Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front. Oncogene. 20:2001;1981-1989.
-
(2001)
Oncogene
, vol.20
, pp. 1981-1989
-
-
Paweletz, C.P.1
-
30
-
-
0035887145
-
Profiling of cancer cells using protein microarrays: Discovery of novel radiation-regulated proteins
-
Sreekumar A., et al. Profiling of cancer cells using protein microarrays: discovery of novel radiation-regulated proteins. Cancer Res. 61:2001;7585-7593.
-
(2001)
Cancer Res.
, vol.61
, pp. 7585-7593
-
-
Sreekumar, A.1
-
31
-
-
18344374893
-
Multiplexed protein profiling on microarrays by rolling-circle amplification
-
Schweitzer B., et al. Multiplexed protein profiling on microarrays by rolling-circle amplification. Nat. Biotechnol. 20:2002;359-365.
-
(2002)
Nat. Biotechnol.
, vol.20
, pp. 359-365
-
-
Schweitzer, B.1
-
32
-
-
0036898579
-
Protein microarrays and proteomics
-
MacBeath G. Protein microarrays and proteomics. Nat. Genet. 32:(Suppl. 1):2002;526-532.
-
(2002)
Nat. Genet.
, vol.32
, Issue.SUPPL. 1
, pp. 526-532
-
-
MacBeath, G.1
-
33
-
-
18544397601
-
Directed evolution of high-affinity antibody mimics using mRNA display
-
Xu L., et al. Directed evolution of high-affinity antibody mimics using mRNA display. Chem. Biol. 9:2002;933-942.
-
(2002)
Chem. Biol.
, vol.9
, pp. 933-942
-
-
Xu, L.1
-
34
-
-
0035003057
-
Protein chips based on recombinant antibody fragments: A highly sensitive approach as detected by mass spectrometry
-
Borrebaeck C.A., et al. Protein chips based on recombinant antibody fragments: a highly sensitive approach as detected by mass spectrometry. BioTechniques. 30:2001;1126-1132.
-
(2001)
BioTechniques
, vol.30
, pp. 1126-1132
-
-
Borrebaeck, C.A.1
-
36
-
-
0027787769
-
Intracellular expression of anti-p21ras single chain Fv fragments inhibits meiotic maturation of Xenopus oocytes
-
Biocca S., et al. Intracellular expression of anti-p21ras single chain Fv fragments inhibits meiotic maturation of Xenopus oocytes. Biochem. Biophys. Res. Commun. 197:1993;422-427.
-
(1993)
Biochem. Biophys. Res. Commun.
, vol.197
, pp. 422-427
-
-
Biocca, S.1
-
37
-
-
0029826880
-
Human recombinant antibody fragments neutralising human immunodeficiency virus type 1 reverse transcriptase provide an experimental basis for the structural classification of the DNA polymerase family
-
Gargano N., et al. Human recombinant antibody fragments neutralising human immunodeficiency virus type 1 reverse transcriptase provide an experimental basis for the structural classification of the DNA polymerase family. J. Virol. 70:1996;7706-7712.
-
(1996)
J. Virol.
, vol.70
, pp. 7706-7712
-
-
Gargano, N.1
-
38
-
-
0030776175
-
Inhibition of murine leukaemia virus retrotranscription by the intracellular expression of a phage-derived anti-reverse transcriptase antibody fragment
-
Gargano N., Cattaneo A. Inhibition of murine leukaemia virus retrotranscription by the intracellular expression of a phage-derived anti-reverse transcriptase antibody fragment. J. Gen. Virol. 78:1997;2591-2599.
-
(1997)
J. Gen. Virol.
, vol.78
, pp. 2591-2599
-
-
Gargano, N.1
Cattaneo, A.2
-
39
-
-
85031151400
-
The mode of action of the Y13-259 scFv fragment when expressed intracellularly in mammalian cells
-
in press
-
Cardinale, A. et al. The mode of action of the Y13-259 scFv fragment when expressed intracellularly in mammalian cells. FEBS Lett. (in press).
-
FEBS Lett.
-
-
Cardinale, A.1
-
40
-
-
0028914482
-
Inhibition of HIV-1 Tat-mediated LTR transactivation and HIV-1 infection by anti-Tat single chain intrabodies
-
Mhashilkar A.M., et al. Inhibition of HIV-1 Tat-mediated LTR transactivation and HIV-1 infection by anti-Tat single chain intrabodies. EMBO J. 14:1995;1542-1551.
-
(1995)
EMBO J.
, vol.14
, pp. 1542-1551
-
-
Mhashilkar, A.M.1
-
41
-
-
0028955535
-
Phenotypic knockout of the high-affinity human interleukin 2 receptor by intracellular single-chain antibodies against the α subunit of the receptor
-
Richardson J.H., et al. Phenotypic knockout of the high-affinity human interleukin 2 receptor by intracellular single-chain antibodies against the α subunit of the receptor. Proc. Natl. Acad. Sci. U. S. A. 92:1995;3137-3141.
-
(1995)
Proc. Natl. Acad. Sci. U. S. A.
, vol.92
, pp. 3137-3141
-
-
Richardson, J.H.1
-
42
-
-
0028024772
-
Intracellular single chain Fv antibody inhibits Ras activity in T-cell antigen receptor stimulated Jurkat cells
-
Werge T.M., et al. Intracellular single chain Fv antibody inhibits Ras activity in T-cell antigen receptor stimulated Jurkat cells. FEBS Lett. 351:1994;393-396.
-
(1994)
FEBS Lett.
, vol.351
, pp. 393-396
-
-
Werge, T.M.1
-
43
-
-
0033952543
-
Diverting a protein from its cellular location by intracellular antibodies. The case of p21Ras
-
Lener M., et al. Diverting a protein from its cellular location by intracellular antibodies. The case of p21Ras. Eur. J. Biochem. 267:2000;1196-1205.
-
(2000)
Eur. J. Biochem.
, vol.267
, pp. 1196-1205
-
-
Lener, M.1
-
44
-
-
0027275371
-
Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gp120 single-chain antibody
-
Marasco W.A., et al. Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gp120 single-chain antibody. Proc. Natl. Acad. Sci. U. S. A. 90:1993;7889-7893.
-
(1993)
Proc. Natl. Acad. Sci. U. S. A.
, vol.90
, pp. 7889-7893
-
-
Marasco, W.A.1
-
45
-
-
85047699270
-
Intrabody-mediated phenotypic knockout of major histocompatibility complex class I expression in human and monkey cell lines and in primary human keratinocytes
-
Mhashilkar A.M., et al. Intrabody-mediated phenotypic knockout of major histocompatibility complex class I expression in human and monkey cell lines and in primary human keratinocytes. Gene Ther. 9:2002;307-319.
-
(2002)
Gene Ther.
, vol.9
, pp. 307-319
-
-
Mhashilkar, A.M.1
-
46
-
-
85031157285
-
Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework
-
in press
-
Auf der Maur A. et al. Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework. J. Biol. Chem. (in press).
-
J. Biol. Chem.
-
-
Auf der Maur, A.1
-
47
-
-
0033499632
-
Extended half-life and elevated steady-state level of a single-chain Fv intrabody are critical for specific intracellular retargeting of its antigen, caspase-7
-
Zhu Q., et al. Extended half-life and elevated steady-state level of a single-chain Fv intrabody are critical for specific intracellular retargeting of its antigen, caspase-7. J. Immunol. Methods. 231:1999;207-222.
-
(1999)
J. Immunol. Methods
, vol.231
, pp. 207-222
-
-
Zhu, Q.1
-
48
-
-
0033520343
-
Intrabody construction and expression. I. The critical role of VL domain stability
-
Ohage E., Steipe B. Intrabody construction and expression. I. The critical role of VL domain stability. J. Mol. Biol. 291:1999;1119-1128.
-
(1999)
J. Mol. Biol.
, vol.291
, pp. 1119-1128
-
-
Ohage, E.1
Steipe, B.2
-
49
-
-
0036299049
-
The intracellular antibody capture technology (IACT): Towards a consensus sequence for intracellular antibodies
-
Visintin M., et al. The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies. J. Mol. Biol. 317:2002;73-83.
-
(2002)
J. Mol. Biol.
, vol.317
, pp. 73-83
-
-
Visintin, M.1
-
50
-
-
0033520452
-
Intrabody construction and expression. II. A synthetic catalytic Fv fragment
-
Ohage E.C., et al. Intrabody construction and expression. II. A synthetic catalytic Fv fragment. J. Mol. Biol. 291:1999;1129-1134.
-
(1999)
J. Mol. Biol.
, vol.291
, pp. 1129-1134
-
-
Ohage, E.C.1
-
51
-
-
0028111743
-
Sequence statistics reliably predict stabilizing mutations in a protein domain
-
Steipe B., et al. Sequence statistics reliably predict stabilizing mutations in a protein domain. J. Mol. Biol. 240:1994;188-192.
-
(1994)
J. Mol. Biol.
, vol.240
, pp. 188-192
-
-
Steipe, B.1
-
52
-
-
0032718607
-
Intrabody construction and expression III: Engineering hyperstable V(H) domains
-
Wirtz P., Steipe B. Intrabody construction and expression III: engineering hyperstable V(H) domains. Protein Sci. 8:1999;2245-2250.
-
(1999)
Protein Sci.
, vol.8
, pp. 2245-2250
-
-
Wirtz, P.1
Steipe, B.2
-
53
-
-
0035823143
-
Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies
-
Bach H., et al. Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies. J. Mol. Biol. 312:2001;79-93.
-
(2001)
J. Mol. Biol.
, vol.312
, pp. 79-93
-
-
Bach, H.1
-
54
-
-
0033865190
-
Engineered protein scaffolds for molecular recognition
-
Skerra A. Engineered protein scaffolds for molecular recognition. J. Mol. Recognit. 13:2000;167-187.
-
(2000)
J. Mol. Recognit.
, vol.13
, pp. 167-187
-
-
Skerra, A.1
-
55
-
-
0028982245
-
A combinatorial library of an α-helical bacterial receptor domain
-
Nord K., et al. A combinatorial library of an α-helical bacterial receptor domain. Protein Eng. 8:1995;601-608.
-
(1995)
Protein Eng.
, vol.8
, pp. 601-608
-
-
Nord, K.1
-
56
-
-
0029876415
-
Genetic selection of peptide aptamers that recognize and inhibit cyclin-dependent kinase 2
-
Colas P., et al. Genetic selection of peptide aptamers that recognize and inhibit cyclin-dependent kinase 2. Nature. 380:1996;548-550.
-
(1996)
Nature
, vol.380
, pp. 548-550
-
-
Colas, P.1
-
57
-
-
0034610373
-
Targeted modification and transportation of cellular proteins
-
Colas P., et al. Targeted modification and transportation of cellular proteins. Proc. Natl. Acad. Sci. U. S. A. 97:2000;13720-13725.
-
(2000)
Proc. Natl. Acad. Sci. U. S. A.
, vol.97
, pp. 13720-13725
-
-
Colas, P.1
-
58
-
-
0032564350
-
An artificial cell-cycle inhibitor isolated from a combinatorial library
-
Cohen B.A., et al. An artificial cell-cycle inhibitor isolated from a combinatorial library. Proc. Natl. Acad. Sci. U. S. A. 95:1998;14272-14277.
-
(1998)
Proc. Natl. Acad. Sci. U. S. A.
, vol.95
, pp. 14272-14277
-
-
Cohen, B.A.1
-
59
-
-
0037173610
-
Modulation of HIV-1 replication by RNA interference
-
Jacque J.M., et al. Modulation of HIV-1 replication by RNA interference. Nature. 418:2002;435-438.
-
(2002)
Nature
, vol.418
, pp. 435-438
-
-
Jacque, J.M.1
-
60
-
-
0036245628
-
U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells
-
Miyagishi M., Taira K. U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nat. Biotechnol. 20:2002;497-500.
-
(2002)
Nat. Biotechnol.
, vol.20
, pp. 497-500
-
-
Miyagishi, M.1
Taira, K.2
-
61
-
-
0037117550
-
A DNA vector-based RNAi technology to suppress gene expression in mammalian cells
-
Sui G., et al. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc. Natl. Acad. Sci. U. S. A. 99:2002;5515-5520.
-
(2002)
Proc. Natl. Acad. Sci. U. S. A.
, vol.99
, pp. 5515-5520
-
-
Sui, G.1
-
62
-
-
0035694237
-
Identification of essential genes in cultured mammalian cells using small interfering RNAs
-
Harborth J., et al. Identification of essential genes in cultured mammalian cells using small interfering RNAs. J. Cell Sci. 114:2001;4557-4565.
-
(2001)
J. Cell Sci.
, vol.114
, pp. 4557-4565
-
-
Harborth, J.1
-
63
-
-
0035942736
-
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
-
Elbashir S.M., et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411:2001;494-498.
-
(2001)
Nature
, vol.411
, pp. 494-498
-
-
Elbashir, S.M.1
|