Intrabody construction and expression III: Engineering hyperstable V(H) domains

Protein Science

Volumn 8, Issue 11, 1999, Pages 2245-2250

  Wirtz, Peter ^a   Steipe, Boris ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

Intrabodies; Protein engineering; Protein stability; Recombinant expression; VH domain

Indexed keywords

DISULFIDE; IMMUNOGLOBULIN; PROTEIN;

ARTICLE; CYTOPLASM; ESCHERICHIA COLI; GENETIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STABILITY; SEQUENCE ANALYSIS;

ESCHERICHIA COLI;

EID: 0032718607     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.11.2245     Document Type: Article

References (27)

1. Bothwell AL, Paskind M, Reth M, Imanishi KT, Rajewsky K, Baltimore D. 1981. Heavy chain variable region contribution to the NPb family of antibodies: Somatic mutation evident in a gamma 2a variable region. Cell 24:625-637.
2. Cattaneo A, Biocca S. 1999. The selection of intracellular antibodies. Trends Biotechnol 17:115-121.
3. Davies J, Riechmann L. 1996. Single antibody domains as small recognition units: Design and in vitro antigen selection of camelized, human VH domains with improved protein stability. Protein Eng 9:531-537.
4. Gargano N, Cattaneo A. 1997. Rescue of a neutralizing anti-viral antibody fragment from an intracellular polyclonal repertoire expressed in mammalian cells. FEBS Lett 414:537-540.
5. Glockshuber R, Schmidt T, Plückthun A. 1992. The disulfide bonds in antibody variable domains: Effects on stability, folding in vitro, and functional expression in Escherichia coll. Biochemistry 31:1270-1279.
6. Guo J, Huang W, Scanlan TS. 1994. Kinetic and mechanistic characterization of an efficient hydrolytic antibody: Evidence for the formation of an acyl intermediate. J Am Chem Soc 116:6062-6069.
7. Hamers-Casterman C, Atarhouch T, Muyldermans S, Robinson G, Hamers C, Songa EB, Bendahman N, Hamers R. 1993. Naturally occurring antibodies devoid of light chains. Nature 363:446-448.
8. Kabat EA, Wu TT, Perry HM, Gottesman KS, Foeller C. 1992. Distribution files of the fifth edition of sequences of proteins of immunological interest. http://immuno.bme.nwu.edu.
9. Lauwereys M, Arbabi Ghahroudi M, Desmyter A, Kinne J, Holzer W, De Genst E, Wyns L, Muyldermans S. 1998. Potent enzyme inhibitors derived from dromedary heavy-chain antibodies. EMBO J 17:3512-3520.
10. Maciejewski JP, Weichold FF, Young NS, Cara A, Zella D, Reitz MJ, Gallo RC. 1995. Intracellular expression of antibody fragments directed against HIV reverse transcriptase prevents HIV infection in vitro. Nature Med 1:667-673.
11. Marasco WA. 1997. Intrabodies: Turning the humoral immune system outside in for intracellular immunization. Gene Ther 4:11-15.
12. Martin F, Volpari C, Steinkühler C, Dimasi N, Brunetti M, Biasiol G, Altamura S, Cortese R, De Francesco R, Sollazzo M. 1997. Affinity selection of a camelized V(H) domain antibody inhibitor of hepatitis C virus NS3 protease. Protein Eng 10:607-614.
13. Martineau P, Jones P, Winter G. 1998. Expression of an antibody fragment at high levels in the bacterial cytoplasm. J Mol Biol 280:117-127.
14. Ohage EC, Steipe B. 1999. Intrabody construction and expression I: The critical role of VL domain stability. J Mol Biol 291:1119-1128.
15. Ohage EC, Wirtz P, Barnikow J, Steipe B. 1999. Intrabody construction and expression II: A synthetic catalytic Fv. J Mol Biol 291:1129-1134.
16. 1 with zero, one, and two intact disulfide bonds. J Biol Chem 263:11820-11825.
17. Proba K, Ge L, Plückthun A. 1995. Functional antibody single-chain fragments from the cytoplasm of Escherichia coli: Influence of thioredoxin reductase (TrxB). Gene 159:203-207.
18. Proba K, Worn A, Honegger A, Plückthun A. 1998. Antibody scFv fragments without disulfide bonds made by molecular evolution. J Mol Biol 275:245-253.
19. Riechmann L. 1996. Rearrangement of the former VL interface in the solution structure of a camelised, single antibody VH domain. J Mol Biol 259:957-969.
20. Rondon IJ, Marasco WA. 1997. Intracellular antibodies (intrabodies) for gene therapy of infectious diseases. Annu Rev Microbiol 51:257-283.
21. Skerra A. 1994. Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli. Gene 151:131-135.
22. v fragment in Escherichia coli. Science 240:1038-1041.
23. Steipe B. 1998. The canonical sequence approximation. http://www.lmb.uni-muenchen.de/groups/bs/canonical.html.
24. Steipe B, Schiller B, Plückthun A, Steinbacher S. 1994. Sequence statistics reliably predict stabilizing mutations in a protein domain. J Mol Biol 240:188-192.
25. Ward ES, Güssow D, Griffiths AD, Jones PT, Winter G. 1989. Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli. Nature 341:544-546.
26. Wu Y, Duan L, Zhu M, Hu B, Kubota S, Bagasra O, Pomerantz RJ. 1996. Binding of intracellular anti-Rev single chain variable fragments to different epitopes of human immunodeficiency virus type 1 rev: Variations in viral inhibition. J Virol 70:3290-3297.
27. Zhou GW, Guo J, Huang W, Fletterick RJ, Scanlan TS. 1994. Crystal structure of a catalytic antibody with a serine protease active site. Science 265:1059-1064.