-
1
-
-
0028291823
-
Isolation of high affinity antibodies directly from large synthetic repertoires
-
Griffiths, A. D., Williams, S. C., Hartley, O., Tomlinson, I. M., Waterhouse, P., Crosby, W. L., Kontermann, R. E., Jones, P. T., Low, N. M., Allison, T. J., Prospero, T. D., Hoogenboom, H. R., Nissim, A., Cox, J. P. L., Harrison, J. L., Zaccolo, M., Gherardi, E., and Winter, G. (1994) Isolation of high affinity antibodies directly from large synthetic repertoires. EMBO J. 13, 3245-3260.
-
(1994)
EMBO J.
, vol.13
, pp. 3245-3260
-
-
Griffiths, A.D.1
Williams, S.C.2
Hartley, O.3
Tomlinson, I.M.4
Waterhouse, P.5
Crosby, W.L.6
Kontermann, R.E.7
Jones, P.T.8
Low, N.M.9
Allison, T.J.10
Prospero, T.D.11
Hoogenboom, H.R.12
Nissim, A.13
Cox, J.P.L.14
Harrison, J.L.15
Zaccolo, M.16
Gherardi, E.17
Winter, G.18
-
2
-
-
0025740577
-
Multi-subunit proteins on the surface of filamentous phage: Methodologies for displaying antibody (Fab) heavy and light chains
-
Hoogenboom, H. R., Griffiths, A. D., Johnson, K. S., Chiswell, D. J., Hudson, P., and Winter, G. (1991) Multi-subunit proteins on the surface of filamentous phage: Methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res. 15, 4133-4137.
-
(1991)
Nucleic Acids Res.
, vol.15
, pp. 4133-4137
-
-
Hoogenboom, H.R.1
Griffiths, A.D.2
Johnson, K.S.3
Chiswell, D.J.4
Hudson, P.5
Winter, G.6
-
3
-
-
0025226085
-
Phage-antibodies: Filamentous phage displaying antibody variable domains
-
McCafferty, J., Griffiths, A. D., Winter, G., and Chiswell, D. J. (1990) Phage-antibodies: Filamentous phage displaying antibody variable domains. Nature 348, 552-554.
-
(1990)
Nature
, vol.348
, pp. 552-554
-
-
McCafferty, J.1
Griffiths, A.D.2
Winter, G.3
Chiswell, D.J.4
-
4
-
-
0028006072
-
Antibody fragments from a single pot phage display library as immunochemical reagents
-
Nissim, A., Hoogenboom, H. R., Tomlinson, I. M., Flynn, G., Midgley, C., Lane, D., and Winter, G. (1994) Antibody fragments from a single pot phage display library as immunochemical reagents. EMBO J. 13, 692-698.
-
(1994)
EMBO J.
, vol.13
, pp. 692-698
-
-
Nissim, A.1
Hoogenboom, H.R.2
Tomlinson, I.M.3
Flynn, G.4
Midgley, C.5
Lane, D.6
Winter, G.7
-
5
-
-
9344223986
-
Human antibodies with subnanomolar affinities isolated from a large non-immunized phage display library
-
Vaughan, T. J., Williams, A. J., Pritchard, K., Osbourn, J. K., Pope, A. R., Earnshaw, J. C., McCafferty, J., Hodits, R. A., Wilton, J., and Johnson, K. (1996) Human antibodies with subnanomolar affinities isolated from a large non-immunized phage display library. Nature Biotechnol. 14, 309-314.
-
(1996)
Nature Biotechnol.
, vol.14
, pp. 309-314
-
-
Vaughan, T.J.1
Williams, A.J.2
Pritchard, K.3
Osbourn, J.K.4
Pope, A.R.5
Earnshaw, J.C.6
McCafferty, J.7
Hodits, R.A.8
Wilton, J.9
Johnson, K.10
-
6
-
-
0023714467
-
A short polypeptide marker sequence for recombinant protein identification and purification
-
Hopp, T. P., Pricket, K. S., Price, V. L., Libby, R. T., March, C. J., Cerretti, D. P., Urdal, D. L., and Conlon, P. J. (1988) A short polypeptide marker sequence for recombinant protein identification and purification. Bio/Technology 6, 1204-1204.
-
(1988)
Bio/Technology
, vol.6
, pp. 1204-1204
-
-
Hopp, T.P.1
Pricket, K.S.2
Price, V.L.3
Libby, R.T.4
March, C.J.5
Cerretti, D.P.6
Urdal, D.L.7
Conlon, P.J.8
-
7
-
-
0030965970
-
Disrupting the hydrophobic patches at the antibody variable/constant domain interface: Improved in vivo folding and physical characterization of an engineered scFv fragment
-
Nieba, L., Honegger, A., Krebber, C., and Plückthun, A. (1997) Disrupting the hydrophobic patches at the antibody variable/constant domain interface: Improved in vivo folding and physical characterization of an engineered scFv fragment. Protein Eng. 10, 435-444.
-
(1997)
Protein Eng.
, vol.10
, pp. 435-444
-
-
Nieba, L.1
Honegger, A.2
Krebber, C.3
Plückthun, A.4
-
8
-
-
14744283859
-
Recombinant colorimetric antibodies: Construction and characterization of a bifunctional F(ab)2/alkaline phosphatase conjugate produced in Escherichia coli
-
Ducancel, F., Gillet, D., Carrier, A., Lajeunesser, E., Menez, A., and Boulain, J. C. (1993) Recombinant colorimetric antibodies: Construction and characterization of a bifunctional F(ab)2/alkaline phosphatase conjugate produced in Escherichia coli. Bio/Technology 11, 601-605.
-
(1993)
Bio/Technology
, vol.11
, pp. 601-605
-
-
Ducancel, F.1
Gillet, D.2
Carrier, A.3
Lajeunesser, E.4
Menez, A.5
Boulain, J.C.6
-
9
-
-
0013627974
-
Antigen detection using recombinant, bifunctional single-chain Fv fusion proteins synthesized in Escherichia coli
-
Gandecha, A. R., Owen, M. R. L., Cockburn, B., and Whitelam, G. C. (1992) Antigen detection using recombinant, bifunctional single-chain Fv fusion proteins synthesized in Escherichia coli. Protein Express. Purif. 5, 361-365.
-
(1992)
Protein Express. Purif.
, vol.5
, pp. 361-365
-
-
Gandecha, A.R.1
Owen, M.R.L.2
Cockburn, B.3
Whitelam, G.C.4
-
10
-
-
0031049705
-
A scFv-alkaline phosphatase fusion-protein which detects potato leafroll luteovirus in plant extracts by ELISA
-
Harper, K., Kerschbaumer, R. J., Ziegler, A., Macintosh, S. M., Cowan, G. H., Himmler, G., and Mayo, M. A. (1997) A scFv-alkaline phosphatase fusion-protein which detects potato leafroll luteovirus in plant extracts by ELISA. J. Virol. Methods 63, 237-242.
-
(1997)
J. Virol. Methods
, vol.63
, pp. 237-242
-
-
Harper, K.1
Kerschbaumer, R.J.2
Ziegler, A.3
Macintosh, S.M.4
Cowan, G.H.5
Himmler, G.6
Mayo, M.A.7
-
11
-
-
0031194670
-
Single-chain Fv fusion proteins suitable as coating and detecting reagents in a double antibody sandwich enzyme-linked immunosorbent assay
-
Kerschbaumer, R. J., Hirschl, S., Kaufman, A., Ibl, M., Koenig, R., and Himmler, G. (1997) Single-chain Fv fusion proteins suitable as coating and detecting reagents in a double antibody sandwich enzyme-linked immunosorbent assay. Anal. Biochem. 249, 219-227.
-
(1997)
Anal. Biochem.
, vol.249
, pp. 219-227
-
-
Kerschbaumer, R.J.1
Hirschl, S.2
Kaufman, A.3
Ibl, M.4
Koenig, R.5
Himmler, G.6
-
12
-
-
0029929019
-
PDAP2: A vector for construction of alkaline phosphatase fusion proteins
-
Kerschbaumer, R. J., Hirschl, S., Schwager, C., Ibl, M., and Himmler, G. (1996) PDAP2: A vector for construction of alkaline phosphatase fusion proteins. Immunotechnology 2, 145-150.
-
(1996)
Immunotechnology
, vol.2
, pp. 145-150
-
-
Kerschbaumer, R.J.1
Hirschl, S.2
Schwager, C.3
Ibl, M.4
Himmler, G.5
-
13
-
-
0026404619
-
Cloning and expression of an HIV-1 specific single-chain Fv region fused to Escherichia coli alkaline phosphatase
-
Kohl, J., Rüker, F., Himmler, G., and Katinger, H. (1990) Cloning and expression of an HIV-1 specific single-chain Fv region fused to Escherichia coli alkaline phosphatase. Ann. N. Y. Acad. Sci. 646, 106-114.
-
(1990)
Ann. N. Y. Acad. Sci.
, vol.646
, pp. 106-114
-
-
Kohl, J.1
Rüker, F.2
Himmler, G.3
Katinger, H.4
-
14
-
-
0031970653
-
Selection of single-chain variable fragment antibodies to black currant reversion associated virus from a synthetic phage display library
-
Susi, P., Ziegler, A., and Torrance, L. (1998) Selection of single-chain variable fragment antibodies to black currant reversion associated virus from a synthetic phage display library. Phytopathology 88, 230-233.
-
(1998)
Phytopathology
, vol.88
, pp. 230-233
-
-
Susi, P.1
Ziegler, A.2
Torrance, L.3
-
15
-
-
0028273913
-
Application of an alkaline phosphatase fusion-protein system suitable for efficient screening and production of Fab-enzyme conjugates in Escherichia coli
-
Weiss, E., and Orfanoudakis, G. (1994) Application of an alkaline phosphatase fusion-protein system suitable for efficient screening and production of Fab-enzyme conjugates in Escherichia coli. J. Biotechnol. 33, 43-53.
-
(1994)
J. Biotechnol.
, vol.33
, pp. 43-53
-
-
Weiss, E.1
Orfanoudakis, G.2
-
16
-
-
14744298086
-
Construction, bacterial expression and characterization of a bifunctional single-chain antibody-phosphatase fusion protein targeted to the human Erbb-2 receptor
-
Wels, W., Harwerth, I., Zwickl, M., Hardman, N., Groner, B., and Hynes, N. E. (1992) Construction, bacterial expression and characterization of a bifunctional single-chain antibody-phosphatase fusion protein targeted to the human Erbb-2 receptor. Bio/Technology 10, 1128-1132.
-
(1992)
Bio/Technology
, vol.10
, pp. 1128-1132
-
-
Wels, W.1
Harwerth, I.2
Zwickl, M.3
Hardman, N.4
Groner, B.5
Hynes, N.E.6
-
18
-
-
0024845655
-
Expression of functional Fv and Fab fragments in Escherichia coli
-
Plückthun, A., and Skerra, A. (1989) Expression of functional Fv and Fab fragments in Escherichia coli. Methods Enzymol. 178, 497-515.
-
(1989)
Methods Enzymol.
, vol.178
, pp. 497-515
-
-
Plückthun, A.1
Skerra, A.2
-
19
-
-
0028030082
-
Mechanisms underlying expression of Tn10 encoded tetracycline resistance
-
Hillen, W., and Berens, C. (1994) Mechanisms underlying expression of Tn10 encoded tetracycline resistance. Annu. Rev. Microbiol. 48, 345-369.
-
(1994)
Annu. Rev. Microbiol.
, vol.48
, pp. 345-369
-
-
Hillen, W.1
Berens, C.2
-
20
-
-
0028555357
-
Use of tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli
-
Skerra, A. (1994) Use of tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli. Gene 151, 131-135.
-
(1994)
Gene
, vol.151
, pp. 131-135
-
-
Skerra, A.1
-
21
-
-
0003044623
-
Purification of native proteins from the cytoplasm and periplasm of Escherichia coli using IMAC and histidine tails: A comparison of proteins and protocols
-
Lindner, P., Guth, B., Wuelfmg, C., Krebber, C., Steipe, B., Mueller, F., and Plückthun, A. (1992) Purification of native proteins from the cytoplasm and periplasm of Escherichia coli using IMAC and histidine tails: A comparison of proteins and protocols. Methods 4, 41-56.
-
(1992)
Methods
, vol.4
, pp. 41-56
-
-
Lindner, P.1
Guth, B.2
Wuelfmg, C.3
Krebber, C.4
Steipe, B.5
Mueller, F.6
Plückthun, A.7
-
22
-
-
0026045194
-
Real-time biospecific interaction analysis using surface plasmon resonance and a sensor chip technology
-
Jönsson, U., Fagerstam, L., Ivarsson, B., Johnsson, B., Karlsson, R., Lundh, K., Lofas., S., Persson, B., Roos, H., Ronnberg, I., and Malmqvist, M. (1991) Real-time biospecific interaction analysis using surface plasmon resonance and a sensor chip technology. Biotechniques 11, 620-627.
-
(1991)
Biotechniques
, vol.11
, pp. 620-627
-
-
Jönsson, U.1
Fagerstam, L.2
Ivarsson, B.3
Johnsson, B.4
Karlsson, R.5
Lundh, K.6
Lofas, S.7
Persson, B.8
Roos, H.9
Ronnberg, I.10
Malmqvist, M.11
-
23
-
-
0013607980
-
Selection of Ralstonia solanacearum race 3 specific monoclonal antibodies from combinatorial libraries
-
Prior, P., Alien, C., and Elphinstone, J., Eds., Springer-Verlag, Berlin
-
Griep, R. A., Van Twisk, C., Van der Wolf, J. M., Van Beckhoven, J. R., and Schots, A. (1998) Selection of Ralstonia solanacearum race 3 specific monoclonal antibodies from combinatorial libraries in "Bacterial Wilt Disease: Molecular and Ecological Aspects" (Prior, P., Alien, C., and Elphinstone, J., Eds.), pp. 140-145, Springer-Verlag, Berlin.
-
(1998)
Bacterial Wilt Disease: Molecular and Ecological Aspects
, pp. 140-145
-
-
Griep, R.A.1
Van Twisk, C.2
Van Der Wolf, J.M.3
Van Beckhoven, J.R.4
Schots, A.5
-
24
-
-
0030861452
-
Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O and the AraC/I1-I2 regulatory elements
-
Lutz, R., and Bujard H. (1997) Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O and the AraC/I1-I2 regulatory elements. Nucleic Acids Res. 25, 1203-1210.
-
(1997)
Nucleic Acids Res.
, vol.25
, pp. 1203-1210
-
-
Lutz, R.1
Bujard, H.2
-
25
-
-
0021402226
-
Construction of an E. coli strain overproducing the Tn10-encoded Tet repressor and its use for large scale purification
-
Oehmichen, R., Klock, G., Altschmied, L., and Hillen, W. (1984) Construction of an E. coli strain overproducing the Tn10-encoded Tet repressor and its use for large scale purification. EMBO J. 3, 539-543.
-
(1984)
EMBO J.
, vol.3
, pp. 539-543
-
-
Oehmichen, R.1
Klock, G.2
Altschmied, L.3
Hillen, W.4
-
26
-
-
0027975979
-
Intra and extracellular expression of a scFv antibody fragment in E. coli: Effect of bacterial strains and pathway engineering using GroES/L chaperonins
-
Dueñas, M., Vázquez, J., Ayala, M., Söderlind, E., Ohlin, M., Pérez, L., Borrebaeck, C. A. K., and Gavilondo, J. V. (1994) Intra and extracellular expression of a scFv antibody fragment in E. coli: Effect of bacterial strains and pathway engineering using GroEs/L chaperonins. BioTechniques 16, 476-483.
-
(1994)
BioTechniques
, vol.16
, pp. 476-483
-
-
Dueñas, M.1
Vázquez, J.2
Ayala, M.3
Söderlind, E.4
Ohlin, M.5
Pérez, L.6
Borrebaeck, C.A.K.7
Gavilondo, J.V.8
-
27
-
-
14744300211
-
By-passing immunization: Building high affinity antibodies by chain shuffling
-
Marks, J. D., Griffiths, A. D., Malmqvist, M., Clackson, T. P., Bye, J. M., and Winter, G. (1992) By-passing immunization: Building high affinity antibodies by chain shuffling. BiotechnolOgy 7, 779-783.
-
(1992)
Biotechnology
, vol.7
, pp. 779-783
-
-
Marks, J.D.1
Griffiths, A.D.2
Malmqvist, M.3
Clackson, T.P.4
Bye, J.M.5
Winter, G.6
-
28
-
-
0028966985
-
Engineered turns of a recombinant antibody improve its in vivo folding
-
Knappik, A., and Plückthun, A. (1995) Engineered turns of a recombinant antibody improve its in vivo folding. Protein Eng. 8, 81-89.
-
(1995)
Protein Eng.
, vol.8
, pp. 81-89
-
-
Knappik, A.1
Plückthun, A.2
-
29
-
-
0030017673
-
Folding nuclei of the scFv fragment of an antibody
-
Freund, C., Honegger, A., Hunziker, P., Holak, T. A., and Plückthun, A. (1996) Folding nuclei of the scFv fragment of an antibody. Biochemistry35, 8457-8464.
-
(1996)
Biochemistry
, vol.35
, pp. 8457-8464
-
-
Freund, C.1
Honegger, A.2
Hunziker, P.3
Holak, T.A.4
Plückthun, A.5
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