RAG1-DNA binding in V(D)J recombination: Specificity and DNA-induced conformational changes revealed by fluorescence and CD spectroscopy

Journal of Biological Chemistry

Volumn 278, Issue 8, 2003, Pages 5584-5596

  Ciubotaru, Mihai ^a   Ptaszek, Leon M ^c   Baker, Gary A ^a   Baker, Sheila N ^b   Bright, Frank V ^b   Schatz, David G ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY AT BUFFALO   (United States)

^c NEW YORK UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANISOTROPY; FLUORESCENCE; POLARIZATION; PROTEINS;

FREQUENCY DOMAIN SPECTROSCOPY;

DNA;

AMINO ACID; DNA; IMMUNOGLOBULIN RECEPTOR; RAG1 PROTEIN; RAG2 PROTEIN; T LYMPHOCYTE RECEPTOR; DNA BINDING PROTEIN; DNA POLYMERASE; HOMEODOMAIN PROTEIN; OLIGODEOXYRIBONUCLEOTIDE; RAG 1 PROTEIN; RAG-1 PROTEIN; RAG2 PROTEIN, MOUSE; RECOMBINANT PROTEIN; RECOMBINASE; TRANSPOSASE; V(D)J RECOMBINATION ACTIVATING PROTEIN 2;

ANISOTROPY; ARTICLE; BINDING AFFINITY; CIRCULAR DICHROISM; COMPLEX FORMATION; CONCENTRATION RESPONSE; FLUORESCENCE; GENETIC RECOMBINATION; HYDROPHOBICITY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DNA BINDING; PROTEIN INTERACTION; ANIMAL; BINDING SITE; CHEMISTRY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE; GENETIC TRANSFECTION; GENETICS; KINETICS; METABOLISM; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PROTEIN CONFORMATION;

MURINAE;

ANIMALS; BASE SEQUENCE; BINDING SITES; CIRCULAR DICHROISM; CLONING, MOLECULAR; DNA; DNA NUCLEOTIDYLTRANSFERASES; DNA-BINDING PROTEINS; ESCHERICHIA COLI; GENES, RAG-1; HOMEODOMAIN PROTEINS; KINETICS; MICE; OLIGODEOXYRIBONUCLEOTIDES; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; SUBSTRATE SPECIFICITY; TRANSFECTION; TRANSPOSASES; VDJ RECOMBINASES;

EID: 0037458557     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M209758200     Document Type: Article

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