Functional organization of single and paired V(D)J cleavage complexes

Molecular and Cellular Biology

Volumn 21, Issue 13, 2001, Pages 4256-4264

  Landree, M A ^a   Kale, S B ^a   Roth, D B ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIMER; MANGANESE; NUCLEOPROTEIN; PROTEIN; PROTEIN RAG 1; PROTEIN RAG 2; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; DNA BINDING; ENZYME ACTIVE SITE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE; SYNAPSE;

BINDING SITES; DIMERIZATION; DNA; DNA NUCLEOTIDYLTRANSFERASES; DNA-BINDING PROTEINS; HOMEODOMAIN PROTEINS; IMMUNOBLOTTING; MAGNESIUM; MANGANESE; NUCLEIC ACID CONFORMATION; PROTEIN BINDING; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; VDJ RECOMBINASES;

EID: 0034972168     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.21.13.4256-4264.2001     Document Type: Article

References (32)

1. Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system
2. Distinct roles of RAG1 and RAG2 in binding the V(D)J recombination signal sequences
3. A RAG1 and RAG2 tetramer complex is active in cleavage in V(D)J recombination
4. The three chemical steps of Tn10/IS10 transposition involve repeated utilization of a single active site
5. RAG1 mediates signal sequence recognition and recruitment of RAG2 in V(D)J recombination
6. Initiation of V(D)J recombination in vitro obeying the 12/23 rule
7. Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complex
8. A stable RAG1-RAG2-DNA complex that is active in V(D)J cleavage
9. Assembly of a 12/23 paired signal complex: A critical control point in V(D)J recombination
10. DNA transposition by the RAG1 and RAG2 proteins: A possible source of oncogenic translocations
11. Single active site catalysis of the successive phosphoryl transfer steps by DNA transposases: Insights from phosphorothioate stereoselectivity
12. Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinase
13. Functional analysis of coordinated cleavage in V(D)J recombination
14. Mutational analysis of RAG-1 and RAG-2 identifies three active site amino acids in RAG-1 critical for both cleavage steps of V(D)J recombination
15. RAG-1 and RAG-2-dependent assembly of functional complexes with V(D)J recombination substrates in solution
16. The defect in murine severe combined immune deficiency: Joining of signal sequences but not coding segments in V(D)J recombination
17. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps
18. The same two monomers within a MuA tetramer provide the DDE domains for the strand cleavage and strand transfer steps of transposition
19. Trans catalysis in Tn5 transposition
20. Separation-of-function mutants reveal critical roles for RAG2 in both the cleavage and joining steps of V(D)J recombination
21. A dimer of the lymphoid protein RAG1 recognizes the recombination signal sequence and the complex stably incorporates the high mobility group protein HMG2
22. Characterization of broken DNA molecules associated with V(D)J recombination
23. 2+ cofactors at the initial stages of V(D)J recombination
24. The Tn7 transposase is a heteromeric complex in which DNA breakage and joining activities are distributed between different gene products
25. V(D)J recombination: Modulation of RAG1 and RAG2 cleavage activity on 12/23 substrates by whole cell extract and DNA-bending proteins
26. The homeodomain region of Rag-1 reveals the parallel mechanisms of bacterial and V(D)J recombination
27. The DDE motif in RAG-1 is contributed in trans to a single active site that catalyzes the nicking and transesterification steps of V(D)J recombination
28. V(D)J recombination signal recognition: Distinct, overlapping DNA-protein contacts in complexes containing RAG1 with and without RAG2
29. RAG-2 promotes heptamer occupancy by RAG-1 in the assembly of a V(D)J initiation complex
30. The RAG1 and RAG2 proteins establish the 12/23 rule in V(D)J recombination
31. EcoRV restriction endonuclease: Communication between catalytic metal ions and DNA recognition
32. Organization and dynamics of the Mu transpososome: Recombination by communication between two active sites