The DDE motif in RAG-1 is contributed in trans to a single active site that catalyzes the nicking and transesterification steps of V(D)J recombination

Molecular and Cellular Biology

Volumn 21, Issue 2, 2001, Pages 449-458

  Swanson, P C ^a  

^a CREIGHTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MAGNESIUM; MANGANESE; RAG1 PROTEIN; RAG2 PROTEIN; RECOMBINASE; UNCLASSIFIED DRUG;

ARTICLE; CARBOXYLATION; DNA PROTEIN COMPLEX; DNA RECOMBINATION; DNA STRAND BREAKAGE; ENZYME ACTIVE SITE; ESTERIFICATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; REACTION ANALYSIS;

AMINO ACID MOTIFS; BINDING SITES; CATALYSIS; DIMERIZATION; DNA; DNA NUCLEOTIDYLTRANSFERASES; DNA-BINDING PROTEINS; GENE REARRANGEMENT; HOMEODOMAIN PROTEINS; HUMANS; IMMUNOGLOBULINS; MUTATION; NUCLEAR PROTEINS; NUCLEIC ACID CONFORMATION; OLIGODEOXYRIBONUCLEOTIDES; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; RECEPTORS, ANTIGEN, T-CELL; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; VDJ RECOMBINASES;

EID: 0035168323     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.21.2.449-458.2001     Document Type: Article

References (52)

1. Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system
2. The RAG1 homeodomain recruits HMG1 and HMG2 to facilitate recombination signal sequence binding and to enhance the intrinsic DNA-bending activity of RAG1-RAG2
3. Distinct roles of RAG1 and RAG2 in binding the V(D)J recombination signal sequences
4. A RAG1 and RAG2 tetramer complex is active in cleavage in V(D)J recombination
5. Hairpin coding end opening is mediated by RAG1 and RAG2 proteins
6. Hairpin formation in Tn5 transposition
7. The three chemical steps of Tn10/IS10 transposition involve repeated utilization of a single active site
8. Three-dimensional structure of the Tn5 synaptic complex transposition intermediate
9. RAG1 mediates signal sequence recognition and recruitment of RAG2 in V(D)J recombination
10. Initiation of V(D)J recombination in vitro obeying the 12/23 rule
11. Detection of RAG protein-V(D)J recombination signal interactions near the site of DNA cleavage by UV cross-linking
12. The RAG proteins and V(D)J recombination: Complexes, ends, and transposition
13. Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complex
14. Integrating DNA: Transposases and retroviral integrases
15. Assembly of a 12/23 paired signal complex: A critical control point in V(D)J recombination
16. A stable RAG1-RAG2-DNA complex that is active in V(D)J cleavage
17. DNA transposition by the RAG1 and RAG2 proteins: A possible source of oncogenic translocations
18. Recombinant circle PCR and recombination PCR for site-specific mutagenesis without PCR product purification
19. Tn10 transposition via a DNA hairpin intermediate
20. Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinase
21. Tn10 and IS10 transposition and chromosome rearrangements: Mechanism and regulation in vivo and in vitro
22. Mutational analysis of RAG1 and RAG2 identifies three catalytic amino acids in RAG1 critical for both cleavage steps of V(D)J recombination
23. Structural aspects of a higher order nucleoprotein complex: Induction of an altered DNA structure at the Mu-host junction of the Mu type 1 transpososome
24. The mechanism of V(D)J joining: Lessons from molecular, immunological, and comparative analyses
25. RAG-1- and RAG-2-dependent assembly of functional complexes with V(D)J recombination substrates in solution
26. Regulation of V(D)J recombination activator protein RAG-2 by phosphorylation
27. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps
28. Rejoining of DNA by the RAG1 and RAG2 proteins
29. The same two monomers within a MuA tetramer provide the DDE domains for the strand cleavage and strand transfer steps of transposition
30. Trans catalysis in Tn5 transposition
31. RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination
32. V(D)J recombination. Ragtime jumping
33. Bacterial transposases and retroviral integrases
34. Tn5: A molecular window on transposition
35. A dimer of the lymphoid protein RAG1 recognizes the recombination signal sequence and the complex stably incorporates the high mobility group protein HMG2
36. VDJ recombination: A transposase goes to work
37. V(D)J recombination: Broken DNA molecules with covalently sealed (hairpin) coding ends in scid mouse thymocytes
38. Characterization of broken DNA molecules associated with V(D)J recombination
39. The RAG1/RAG2 complex constitutes a 3′ flap endonuclease: Implications for junctional diversity in V(D)J and transpositional recombination
40. The Tn7 transposase is a heteromeric complex in which DNA breakage and joining activities are distributed between different gene products
41. The V(D)J recombination activating gene, RAG-1
42. Double-strand signal sequence breaks in V(D)J recombination are blunt, 5′-phosphorylated, RAG-dependent, and cell cycle regulated
43. V(D)J recombination: Signal and coding joint resolution are uncoupled and depend on parallel synapsis of the sites
44. DNA hairpin opening mediated by the RAG1 and RAG2 proteins
45. The homeodomain region of Rag-1 reveals the parallel mechanisms of bacterial and V(D)J recombination
46. RAG-2 promotes heptamer occupancy by RAG-1 in the assembly of a V(D)J initiation complex
47. V(D)J recombination signal recognition: Distinct, overlapping DNA-protein contacts in complexes containing RAG1 with and without RAG2
48. Stimulation of V(D)J cleavage by high mobility group proteins
49. Initiation of V(D)J recombination in a cell-free system
50. Similarities between initiation of V(D)J recombination and retroviral integration
51. The RAG1 and RAG2 proteins establish the 12/23 rule in V(D)J recombination
52. Organization and dynamics of the Mu transpososome: Recombination by communication between two active sites