Recent developments in molecular genetics of Candida albicans

Annual Review of Microbiology

Volumn 54, Issue , 2000, Pages 463-498

  De Backer, M D ^a   Magee, P T ^a   Pla, J ^a  

^a JANSSEN RESEARCH FOUNDATION   (Belgium)

Author keywords

Expression; Gene function; Gene manipulation; Genetics; Genome

Indexed keywords

CANDIDA ALBICANS; GENE DISRUPTION; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE INACTIVATION; GENE SILENCING; GENETIC VARIABILITY; IMMUNE DEFICIENCY; MOLECULAR GENETICS; NONHUMAN; OPPORTUNISTIC INFECTION; PRIORITY JOURNAL; PROTEIN LOCALIZATION; REPORTER GENE; REVIEW;

CANDIDA ALBICANS; CANDIDIASIS; GENE EXPRESSION; GENOME, FUNGAL; HUMANS; MOLECULAR BIOLOGY; RECOMBINANT PROTEINS; VARIATION (GENETICS);

EID: 0033765755     PISSN: 00664227     EISSN: None     Source Type: Book Series    
DOI: 10.1146/annurev.micro.54.1.463     Document Type: Review

References (166)

1. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains
2. Simultaneous fluorescence- activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins
3. Gene regulation by antisense RNA in the fission yeast Schizosaccharomyces pombe
4. Antisense gene expression in yeast
5. Artificial ribozyme and antisense gene expression in S. cerevisiae
6. The ade6 gene of the fission yeast as a target for antisense and ribozyme RNA-mediated suppression
7. The Candida albicans HYR1 gene, which is activated in response to hyphal development, belongs to a gene family encoding yeast cell wall proteins
8. Isolation, characterization, and genetic analysis of monosomic, aneuploid mutants of Candida albicans
9. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae
10. Electroporation protocols for microorganisms
11. High-efficiency transformation of yeast by electroporation
12. Secular trends in the epiderniology of nosocomial fungal infections in the United States, 1980-1990: National Nosocomial Infections Surveillance System
13. An activator/repressor dual system allows fight tetracycline-regulated gene expression in budding yeast
14. Antimicrobial agents: Antifungal agents
15. Structure and regulation of the Candida albicans ADH1 gene encoding an immunogenic alcohol dehydrogenase
16. The emergence of fungi as major pathogens
17. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance
18. Thirteen-year evolution of azole resistance in yeast isolates and prevalence of resistant strains carried by cancer patients at a large medical center
19. Control of filament formation in Candida albicans by the transcriptional repressor TUP1
20. Microsatellite polymorphism in the promoter sequence of the elongation factor
21. Stable transformation and regulated expression of an inducible reporter construct in Candida albicans using restriction enzyme-mediated integration
22. Cloning and expression of Candida albicans ADE2 and proteinase genes on a replicative plasmid in C. albicans and in Saccharomyces cerevisiae
23. Molecular cloning and expression of the Candida albicans beta-N-acetylglucosaminidase (HEX1) gene
24. The MET3 promoter: A new tool for Candida albicans molecular genetics
25. Cloning the centromere from Candida albicans using chromosome engineering techniques
26. A physical map of chromosome
27. Construction of an SfiI macrorestriction map of the Candida albicans genome
28. Green fluorescent protein as a reporter of transcription and protein localization in fungi
29. Yeast-enhanced green fluorescent protein (yEGFP) a reporter of gene expression in Candida albicans
30. FACS-optimized mutants of the green fluorescent protein (GFP)
31. Candida pathogenesis: Unravelling the threads of infection
32. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15
33. Roles of the Candida albicans mitogen-activated protein kinase homolog, Cek1p, in hyphal development and systemic candidiasis
34. Putative virulence factors of Candida albicans
35. Transformation of Candida albicans by electroporation
36. PCR- and ligation-mediated synthesis of split-marker cassettes with long flanking homology regions for gene disruption in Candida albicans
37. Morphogenesis-independent regulation of actin transcript levels in the pathogenic yeast Candida albicans
38. Transformation of Saccharomyces cerevisiae by electroporation
39. Positive selection system to screen for inhibitors of human immunodeficiency virus-1 transcription
40. Can we prevent azole resistance in fungi?
41. European Functional Analysis Network (EUROFAN) and the functional analysis of the Saccharomyces cerevisiae genome
42. Highly-efficient electrotransformation of the yeast Hansenula polymorpha
43. New vectors for combinatorial deletions in yeast chromosomes and for gap-repair cloning using 'split-marker' recombination
44. 'Mass-murder' of ORFs from three regions of chromosome XI from Saccharomyces cerevisiae
45. Cell cycle arrest promotes transhammerhead ribozyme action in yeast
46. Common themes in microbial pathogenicity revisited
47. Opportunistic candidiasis: An epidemic of the 1980s
48. Isogenic strain construction and gene mapping in Candida albicans
49. Fungal infection rates are increasing
50. Cloning of a yeast gene for trichodermin resistance and ribosomal protein
51. Linkage of adhesion, filamentous growth, and virulence in Candida albicans to a single gene, INT1
52. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae
53. Cloning and characterization of a Candida albicans maltase gene involved in sucrose utilization
54. The SPA2 gene of Saccharomyces cerevisiae is important for pheromone-induced morphogenesis and efficient mating
55. Human mycoses: Drags and targets for emerging pathogens
56. Antifungal agents: Chemotherapeutic targets and immunological strategies
57. Phenotypic characterization of a Candida albicans strain deficient in its major exoglucanase
58. Repeated use of GALl for gene disruption in Candida albicans
59. A hyphal-specific chitin synthase gene (CHS2) is not essential for growth, dimorphism, or virulence of Candida albicans
60. Key genes in the regulation of dimorphism of Candida albicans
61. The future of antifungal therapy
62. Clinical pharmacology of systemic antifungal agents: A comprehensive review of agents in clinical use, current investigational compounds, and putative targets for antifungal drag development
63. A new efficient gene disruption cassette for repeated use in budding yeast
64. Induced expression of the Candida albicans multidrug resistance gene CDR1 in response to fluconazole and other antifungals
65. A reorganized Candida albicans DNA sequence promoting homologous non-integrative genetic transformation
66. Integrative transformation of yeast using electroporation
67. Transformation of yeast
68. Virulence factors of medically important fungi
69. Candida albicans ALS1: Domains related to a S. cerevisiae sexual agglutinin separated by a repeating motif
70. Identification of a mating type-like locus in the asexual pathogenic yeast Candida albicans
71. Transformation of intact yeast cells treated with alkali cations
72. Isolation and characterization of a repeated sequence (RPS1) of Candida albicans
73. The search for new antifungal drugs and strategies continues
74. Advances in mycobacterial genetics: New promises for old diseases
75. The topoisomerase I gene from Candida albicans
76. Emergence of azole chug resistance in Candida species from HIV-infected patients receiving prolonged fluconazole therapy for oral candidosis
77. Efficient electropulse transformation of intact Candida maltosa cells by different homologous vector plasmids
78. One-step gene disruption by cotransformation to isolate double auxotrophs in Candida albicans
79. Directed mutagenesis in Candida albicans: One-step gene disruption to isolate ura3 mutants
80. Site-specific recombinases: Tools for genome engineering
81. Pathogenicity of Candida albicans auxotrophic mutants in experimental infections
82. Candida albicans hyphal formation and virulence: Is there a clearly defined role?
83. Overexpression of a cloned IMP dehydrogenase gene of Candida albicans confers resistance to the specific inhibitor mycophenolic acid
84. A transformation system for the yeast Candida utilis: Use of a modified endogenous ribosomal protein gene as a drug-resistant marker and ribosomal DNA as an integration target for vector DNA
85. Development of autonomously replicating plasmids for Candida albicans
86. The molecular genetics of Candida albicans
87. Isolation of hem3 mutants from Candida albicans by sequential gene disruption
88. Gene disruption to evaluate the role of fungal Candidate virulence genes
89. A method for performing precise alterations in the yeast genome using a recycable selectable marker
90. Altered expression of selectable marker URA3 in gene-disrupted Candida albicans strains complicates interpretation of virulence studies
91. Beta-galactosidase of Kluyverorayces lactis (Lac4p) as reporter of gene expression in Candida albicans and C. tropicalis
92. Sequence and promoter regulation of the PCK1 gene encoding phosphoenolpyruvate carboxykinase of the fungal pathogen Candida albicans
93. Suppression of hyphal formation in Candida albicans by mutation of a STE12 homolog
94. The molecular genetics of Candida albicans
95. Systemically administered antifungal agents: A review of their clinical pharmacology and therapeutic applications
96. Which came first: The hypha or the yeast?
97. Comparison of molecular typing methods for Candida albicans
98. WO-2, a stable aneuploid derivative of Candida albicans strain WO-1, can switch from white to opaque and form hyphae
99. Selection for bacterial genes that are specifically induced in host tissues: The hunt for virulence factors
100. Selection of bacterial virulence genes that are specifically induced in host tissues
101. High efficiency transformation of Saccharomyces cerevisiae by electroporation
102. pCa1, a highly unusual Ty1/copia retrotransposon from the pathogerdc yeast Candida albicans
103. Translation elongation factor 2 is encoded by a single essential gene in Candida albicans
104. Genomics and antimicrobial drag discovery
105. Expression of a chromosomally integrated, single-copy GFP gene in Candida albicans, and its use as a reporter of gene regulation
106. Sequential gene disruption in Candida albicans by FLP-mediated site-specific recombination
107. Use of URA3 as a reporter of gene expression in C. albicans
108. A controllable gene-expression system for the pathogenis fungus Candida glabrata
109. Artificial antisense regulation of YBR1012, an essential gene from S. cerevisiae which is important for progression through G1/S
110. YBR1012, an essential gene from S. cerevisiae: Construction of an RNA-antisense conditional allele and isolation of a multicopy suppressor
111. Functional characterization of the MKC1 gene of Candida albicans, which encodes a mitogen-activated protein kinase homolog related to cell integrity
112. Cloning, analysis and one-step disruption of the ARG5,6 gene of Candida albicans
113. KEX2 influences Candida albicans proteinase secretion and hyphal formation
114. The changing face of candidemia: Emergence of non-Candida albicans species and antifungal resistance
115. Promoter selectivity of E. coli RNA polymerase: Analysis of the promoter system of convergently-transcribed dnaQ-mh genes
116. Candida species and virulence
117. Recombinase-mediated gene activation and site-specific integration in mammalian cells
118. Characterization of the Candida albicans TRP1 gene and construction of a homozygous trp1 mutant by sequential cotransformation
119. Specific chromosome alterations in fluconazole-resistant mutants of Candida albicans
120. Understanding Candida albicans at the molecular level
121. Cloning of the Candida albicans HIS1 gene by direct complementation of a C. albicans histidine auxotroph using an improved double-ARS shuttle vector
122. Multiple tandem integrations of transforming DNA sequences in yeast chromosomes suggest a mechanism for integrative transformation by homologous recombination
123. Resistant candidia sis
124. A Candida albicans chaperonin subunit (CaCct8p) as a suppressor of morphogenesis and Ras phenotypes in C. albicans and Saccharomyces cerevisiae
125. Tools for functional analysis in C. albicans
126. A Ste6p/P-glycoprotein homologue from the asexual yeast C. albicans transports the a-factor mating pheromone in S. cerevisiae
127. Resistance of Candida species to fluconazole
128. Opaque-white phenotype transition: A programmed morphological transition in Candida albicans
129. A multipurpose transposon system for analyzing protein production, localization, and function in Saccharomyces cerevisiae
130. Variation in assimilating functions occurs in spontaneous Candida albicans mutants having chromosomal alterations
131. Candida albicans: Do mycelia matter?
132. A G-protein alpha subunit from asexual Candida albicans functions in the mating signal transduction pathway of Saccharomyces cerevisiae and is regulated by the al-alpha-2 repressor
133. Site-specific recombinases: Changing partners and doing the twist
134. The Flp recombinase of the 2-micron plasmid of Saccharomyces cerevisiae
135. Susceptibilities of Candida albicans multidrug transporter mutams to various antifungal agents and other metabolic inhibitors
136. The mitogen-activated protein kinase homolog HOG1 gene controls glycerol accumulation in the pathogenic fungus Candida albicans
137. Non-standard translational events in Candida albicans mediated by an unusual seryl-tRNA with a 5'-CAG-3' (leucine) anticodon
138. The CUG codon is decoded in vivo as serine and not leucine in Candida albicans
139. PHR1, a pH-regulated gene of Candida albicans, is required for morphogenesis
140. Genetics of Candida albicans
141. Application of DNA typing methods to epidemiology and taxonomy of Candida species
142. Major trends in the microbial etiology of nosocomial infection
143. Failure of fluconazole in systemic candidiasis
144. Transformation of intact yeast cells by electroporation
145. In vivo expression technology for selection of bacterial genes specifically induced in host tissues
146. Fluconazole resistant Candida in AIDS
147. Genetic footprinting: A genomic strategy for determining a gene's function given its sequence
148. The sea pansy Renilla reniformis luciferase serves as a sensitive bioluminescent reporter for differential gene expression in Candida albicans
149. The frequency of integrative transformation at phase-specific genes of Candida albicans correlates with their transcriptional state
150. Host-induced, stage-specific virulence gene activation in Candida albicans during infection
151. Efg1p, an essential regulator of morphogenesis of the human pathogen Candida albicans, is a member of a conserved class of bHLH proteins regulating morphogenetic processes in fungi
152. Sequence analysis and expression of the two genes for elongation factor 1 alpha from the dimorphic yeast Candida albicans
153. Structure and regulation of the HSP90 gene from the pathogenic fungus Candida albicans
154. An improved protocol for the preparation of yeast cells for transformation by electroporation
155. Sequence analysis of Candida albicans phosphoribosyl-aminoimidazole carboxylase (ADE2) gene
156. Chemotherapy of fungal infections
157. Cloning the centromere from Candida albicans using chromosome engineering techniques
158. Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae
159. New heterologous modules for classical or PCR- based gene disruptions in Saccharomyces cerevisiae
160. The presence of an R467K amino acid substitution and loss of allelic variation correlate with an azoleresistant lanosterol 14alpha demethylase in Candida albicans
161. The 'universal' leucine codon CTG in the secreted aspartyl proteinase 1 (SAP1) gene of Candida albicans encodes a serine in vivo
162. Clinical, cellular, and moleculars factors that contribute to antifungal drug resistance
163. Rapid hypothesis testing with Candida albicians through gene disruption with short homology regions
164. Cloning and sequencing of a Candida albicans catalase gene and effects of disruption of this gene