PCR- and ligation-mediated synthesis of split-marker cassettes with long flanking homology regions for gene disruption in Candida albicans

BioTechniques

Volumn 28, Issue 6, 2000, Pages 1112-1116

  De Hoogt, Ronald ^a   Luyten, Walter H M L ^a   Contreras, Roland ^b   De Backer, Marianne D ^a  

^a JANSSEN RESEARCH FOUNDATION   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

ALLELE; ARTICLE; CANDIDA ALBICANS; CLONING; GENE AMPLIFICATION; GENE CASSETTE; GENE DISRUPTION; MARKER GENE; NONHUMAN; POLYMERASE CHAIN REACTION; SYNTHESIS;

CANDIDA ALBICANS;

EID: 0034125057     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00286st01     Document Type: Article

References (16)

1. Boeke, J.D., F. LaCroute and G.R. Fink. 1984. A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol. Gen. Genet. 197:345-346.
2. De Backer, M.D., R.A. de Hoogt, G. Froyen, F.C. Odds, R. Contreras and W.H.M.L. Luyten. 2000. Single allele knock-out of mRNA capping enzyme leads to unexpected drug and temperature resistance in Candida albicans. Microbiology 146:353-365.
3. De Backer, M.D., D. Maes, S. Vandoninck, M. Logghe, R. Contreras and W.H.M.L. Luyten. 1999. Transformation of Candida albicans by electroporation. Yeast 15:1609-1613.
4. Dujon, B. 1998. European Functional Analysis Network (EUROFAN) and the functional analysis of the Saccharomyces cerevisiae genome. Electrophoresis 19:617-624.
5. Fairhead, C., B. Llorente, F. Denis, M. Soler and B. Dujon. 1996. New vectors for combinatorial deletions in yeast chromosomes and for gap-repair cloning using "split-marker" recombination. Yeast 12:1439-1457.
6. Fairhead, C., A. Thierry, F. Denis, M. Eck and B. Dujon. 1998. "Mass-murder" of ORFs from three regions of chromosome XI from Saccharomyces cerevisiae. Gene 225:33-46.
7. Fonzi, W.A. and M.Y. Irwin. 1993. Isogenic strain construction and gene mapping in Candida albicans. Genetics 134:717-728.
8. Jack, D.B. 1998. The search for new antifungal drugs and strategies continues. Drug News Perspect. 11:306-309.
9. Kirsch, D.R., R. Kelly and M. B. Kurtz. 1990. The Genetics of Candida. CRC Press, Boca Raton, FL.
10. Livak, K.J., S.J. Flood, J. Marmaro, W. Giusti and K. Deetz. 1995. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. Genome Res. 4:357-362.
11. Nikawa, J. and M. Kawabata. 1998. PCR- and ligation-mediated synthesis of marker cassettes with long flanking homology regions for gene disruption in Saccharomyces cerevisiae. Nucleic Acids Res. 26:860-861.
12. Orlando, C., P. Pinzani and M. Pazzagli. 1998. Developments in quantitative PCR. Clin. Chem. Lab. Med. 36:255-269.
13. Plessis, A. and B. Dujon. 1993. Multiple tandem integrations of transforming DNA sequences in yeast chromosomes suggest a mechanism for integrative transformation by homologous recombination. Gene 134:41-50.
14. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning. CSH Laboratory Press, Cold Spring Harbor, NY.
15. Wilson, R.B., D. Davis, B.M. Enloe and A.P. Mitchell. 2000. A recyclable Candida albicans URA3 cassette for PCR product-directed gene disruptions. Yeast 16:65-70.
16. Wilson, R.B., D. Davis and A.P. Mitchell. 1999. Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions. J. Bacteriol. 181:1868-1874.