Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae

Yeast

Volumn 13, Issue 11, 1997, Pages 1065-1075

  Wach, Achim ^a   Brachat, Arndt ^a   Alberti Segui, Christina ^b   Rebischung, Corinne ^a   Philippsen, Peter ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

^b UNIVERSITÉ DE STRASBOURG   (France)

Author keywords

Geneticin; Green fluorescence protein; HIS3 heterologous auxotrophic marker; KanMX; PCR targeting; PFA plasmids

Indexed keywords

ANTIBIOTIC G 418; DNA POLYMERASE; FUNGAL DNA; GREEN FLUORESCENT PROTEIN;

ARTICLE; CELL TRANSFORMATION; CONTROLLED STUDY; FUNGUS MUTATION; GENE CONVERSION; GENETIC RECOMBINATION; MARKER GENE; NONHUMAN; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

ACYLTRANSFERASES; CELL CYCLE PROTEINS; CHROMOSOME MAPPING; CLONING, MOLECULAR; GENES, REPORTER; GENETIC COMPLEMENTATION TEST; GENTAMICINS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; OPEN READING FRAMES; PEPTIDE ELONGATION FACTOR 1; PEPTIDE ELONGATION FACTORS; PLASMIDS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); RECOMBINATION, GENETIC; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SCHIZOSACCHAROMYCES; TRANSCRIPTION FACTORS;

AEQUOREA VICTORIA; EREMOTHECIUM GOSSYPII; FUNGI; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES KLUYVERI; SACCHAROMYCES POMBE; SCHIZOSACCHAROMYCES POMBE; VICTORIA;

EID: 0030840519     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(19970915)13:11<1065::AID-YEA159>3.0.CO;2-K     Document Type: Article

References (41)

1. Amberg, D. C., Botstein, D. and Beasley, E. M. (1995). Precise gene disruption in Saccharomyces cerevisiae by double fusion polymerase chain reaction. Yeast 11, 1275-1280.
2. Barnes, W. M. (1994). PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc. Natl. Acad. Sci USA 91, 2216-2220.
3. Baudin, A., Ozier, K. O., Denouel, A., Lacroute, F. and Cullin, C. (1993). A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucl. Acids Res. 21, 3329-3330.
4. Brachat, A. (1997). Functional Analysis of novel Saccharomyces cerevisiae genes. PhD-Thesis, University of Basel.
5. Bullock, W. O., Fernandez, J. M. and Short, J. M. (1987). XLI-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection. BioTechniques 5, 376-378.
6. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W. and Prasher, D. C. (1994). Green fluorescent protein as a marker for gene expression. Science 263, 802-805.
7. Delagrave, S., Hawtin, R. E., Silva, C. M., Yang, M. M. and Youvan, D. C. (1995). Red-shifted excitation mutants of the green fluorescent protein. Bio/ Technology 13, 151-154.
8. Eberhardt, I. and Hohmann, S. (1995). Strategy for deletion of complete open reading frames in Saccharomyces cerevisiae. Curr. Genet. 27, 306-308.
9. Ehrig, T., O'Cane, D. J. and Prendergast, F. G. (1995). Green fluorescent protein mutants with altered fluorescence excitation spectra. FEBS Lett. 367, 163-166.
10. Erickson, F. L. and Hannig, E. M. (1995). Characterization of Schizosaccharomyces pombe his1 and his5 cDNA. Yeast 11, 157-167.
11. Gietz, R. D. and Woods, R. A. (1994). High efficiency transformation with lithium acetate. In Johnson, J. R. (Ed.), Molecular Genetics of Yeast, A Practical Approach. IRL Press, Oxford, UK, pp. 121-134.
12. Güldener, U., Heck, S., Fielder, T., Beinhauer, J. and Hegemann, J. H. (1996). A new efficient gene disruption cassette for repeated use in budding yeast. Nucl. Acids Res. 24, 2519-2524.
13. Halme, A., Michelitch, M., Mitchell, E. L. and Chant, J. (1996). Bud10p directs axial cell polarization in budding yeast and resembles a transmembrane receptor. Curr. Biol. 6, 570-579.
14. Heery, D. M., Gannon, F. and Powell, R. (1990). A simple method for subcloning DNA fragments from gel slices. Trends Genetics 6, 173.
15. Heim, R., Cubitt, A. B. and Tsien, R. Y. (1995). Improved green fluorescence. Nature 373, 663-664.
16. Heim, R., Prasher, D. C. and Y., T. R. (1994). Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. USA 91, 12501-12504.
17. Heim, R. and Tsien, R. Y. (1996). Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescent resonance energy transfer. Curr. Biol. 6, 178-182.
18. Horton, R. M. (1995). PCR-mediated recombination and mutagenesis. SOEing together tailor-made genes. Mol. Biotechnol. 3, 93-99.
19. Huxley, C., Green, E. D. and Dunham, I. (1990). Rapid assessment of S. cerevisiae mating type by PCR. Trends Genet. 6, 236.
20. Kahana, J. A., Schnapp, B. J. and Silver, P. A. (1995). Kinetics of spindle pole body separation in budding yeast. Proc. Natl. Acad. Sci. USA 92, 9707-9711.
21. Laengle-Ronault, F. and Jacobs, E. (1995). A method for performing precise alterations in the yeast genome usina a recyclable selectable marker. Nucl. Acids Res. 23, 3079-3081.
22. Lorenz, M. C., Muir, R. S., Lim, E., McElver, J., Weber, S. C. and Heitman, J. (1995). Gene disruption with PCR products in Saccharomyces cerevisiae. Gene 158, 113-117.
23. Maftahi, M., Gaillardin, C. and Nicaud, J.-M. (1996). Sticky-end polymerase chain reaction method for systematic gene disruption in Saccharomyces cerevisiae. Yeast 12, 859-868.
24. Mallet, L. and Jacquet, M. (1996). Intergenic flip flop, a method for systematic gene disruption and cloning in yeast. Yeast 12, 1351-1357.
25. Manivasakam, P., Weber, S. C., McElver, J. and Schiestl, R. H. (1995). Micro-homology mediated PCR-targeting in Saccharomyces cerevisiae. Nucl. Acids Res. 23, 2799-2800.
26. McElver, J. and Weber, S. (1992). Flag N-terminal epitope overexpression of bacterial alkaline phosphatase and Flag C-terminal epitope tagging by PCR one-step targeted integration. Yeast 8 (special issue), S627.
27. Mohr, C. (1995). Genetic Engineering of the Filamentous Fungus Ashbya gossypii: Construction of a genomic Library, Isolation of Genes for Isopropylmalate Dehydrogenase (LEU2) and a Protein Kinase (APK1) by Heterologous Complementation and Characterization of Non-reverting Mutants. University of Basel.
28. Niedenthal, R. K., Riles, L., Johnston, M. and Hegemann, J. H. (1996). Green fluorescent protein as a marker for gene expression and subcellular localization in budding yeast. Yeast 12, 773-786.
29. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
30. Scherer, S. and Davis, R. W. (1979). Replacement of chromosome segments with altered DNA sequences constructed in vitro. Proc. Natl. Acad. Sci. USA 76, 4951-4955.
31. Stearns, T. (1995). The green revolution. Curr. Biol. 5, 262-264.
32. Steiner, S. and Philippsen, P. (1994). Sequence and promoter analysis of the highly expressed TEF gene of the filamentous fungus Ashbya gossypii. Mol. Gen. Genet. 242, 263-271.
33. Struhl, K., Cameron, J. R. and Davis, R. W. (1976). Functional genetic expression of eukaryotic DNA in Escherichia coli. Proc. Natl. Acad. Sci. USA 73, 1471-1475.
34. Thomas, B. J. and Rothstein, R. (1989). Elevated recombination rates in transcriptionally active DNA. Cell 56, 619-630.
35. Treco, D. A. (1989). Basic Techniques of Yeast Genetics. Greene Publishing and Wiley-Interscience, New York, 13.1.1-13.1.7 p.
36. Wach, A. (1996). PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae. Yeast 12, 259-265.
37. Wach, A., Brachat, A., Poehlmann, R. and Philippsen, P. (1994). New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10, 1793-1808.
38. Waddle, J. A., Karpova, T. S., Waterston, R. H. and Cooper, J. A. (1996). Movement of cortical patches in yeast. J. Cell. Biol. 132, 861-870.
39. Weinstock, K. G. and Strathern, J. N. (1993). Molecular genetics in Saccharomyces kluyveri: the HIS3 homolog and its use as a selectable marker gene in S. kluyveri and Saccharomyces cerevisiae. Yeast 9, 351-361.
40. Winston, F., Dollard, C. and Ricupero-Hovasse, S. L. (1995). Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C. Yeast 11, 53-55.
41. Wozniak, R. W., Blobel, G. and Rout, M. P. (1994). POM152 is an integral protein of the pore membrane domain of the yeast nuclear envelope. J. Cell. Biol. 125, 31-42.