Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2

ACS Synthetic Biology

Volumn 8, Issue 4, 2019, Pages 796-806

  Roth, Timothy B ^a,b,c   Woolston, Benjamin M ^d   Stephanopoulos, Gregory ^d   Liu, David R ^a,b,c  

^a BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^b HARVARD UNIVERSITY   (United States)

^c HARVARD UNIVERSITY   (United States)

^d Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

directed evolution; methanol assimilation; methanol dehydrogenase; phage assisted continuous evolution; phage assisted noncontinuous evolution; synthetic methylotrophy

Indexed keywords

ALCOHOL DEHYDROGENASE; CARBON 13; FORMALDEHYDE; METHANOL DEHYDROGENASE; METHANOL DEHYDROGENASE 2; UNCLASSIFIED DRUG; ALCOHOL DEHYDROGENASE (ACCEPTOR); BACTERIAL PROTEIN; METHANOL; NICOTINAMIDE ADENINE DINUCLEOTIDE;

ANIMAL MODEL; ARTICLE; BACILLUS; BACILLUS METHANOLICUS; BACTERIAL CELL; BACTERIAL METABOLISM; BACTERIOPHAGE; ENZYME ACTIVITY; EVOLUTION; METABOLITE; MUTATION; NONHUMAN; PHAGE ASSISTED NONCONTINUOUS EVOLUTION; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; METABOLIC ENGINEERING; METABOLISM; PROCEDURES;

ALCOHOL OXIDOREDUCTASES; BACILLUS; BACTERIAL PROTEINS; BACTERIOPHAGES; ESCHERICHIA COLI; FORMALDEHYDE; GENE EXPRESSION REGULATION, BACTERIAL; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; METHANOL; NAD;

EID: 85064645919     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.8b00481     Document Type: Article

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