Industrial biomanufacturing: The future of chemical production

Science

Volumn 355, Issue 6320, 2017, Pages

  Clomburg, James M ^a   Crumbley, Anna M ^a   Gonzalez, Ramon ^a  

^a Rice University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INDUSTRIAL CHEMICAL; BIOFUEL; ENZYME; METHANE;

BIOLOGICAL PRODUCTION; CATALYST; CHEMICAL INDUSTRY; COST-BENEFIT ANALYSIS; ENERGY EFFICIENCY; INDUSTRIAL ENTERPRISE; MANUFACTURING; RESOURCE DEVELOPMENT;

ALCOHOL PRODUCTION; AUTOMATION; BIOCATALYST; BIOPROCESS; BIOTECHNOLOGICAL PRODUCTION; BIOTECHNOLOGY; BIOTRANSFORMATION; ECONOMIC MODEL; ENERGY COST; ENERGY YIELD; GEOGRAPHIC MAPPING; INDUSTRIAL PRODUCTION; MANUFACTURING INDUSTRY; METABOLIC ENGINEERING; NONHUMAN; OIL INDUSTRY; PRIORITY JOURNAL; PROCESS OPTIMIZATION; REVIEW; BIOENGINEERING; CHEMICAL INDUSTRY; CHEMISTRY; FORECASTING; METABOLISM; PROCEDURES; TRENDS;

BIOENGINEERING; BIOFUELS; CHEMICAL INDUSTRY; ENZYMES; FORECASTING; METABOLIC NETWORKS AND PATHWAYS; METHANE;

EID: 85009247636     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.aag0804     Document Type: Review

References (122)

1. SelectUSA, Chemical Spotlight: The Chemical Industry in the United States (International Trade Administration, 2016); https://www.selectusa.gov/chemical-industry-united-states.
2. UNEP, The Global Chemicals Outlook: Towards Sound Management of Chemicals (United Nations Environment Programme, Nairobi, Kenya, 2012).
3. F. Aftalion, A History of the International Chemical Industry (Chemical Heritage Press, 2001; https://books.google.com/books?id=zTP1MFJw8CsC.
4. K. Torén, P. D. Blanc, The history of pulp and paper bleaching: Respiratory-health effects. Lancet 349, 1316-1318 (1997). doi: 10.1016/S0140-6736(96)10141-0; pmid: 9142078
5. K. H. Wolff, Textile bleaching and the birth of the chemical industry. Bus. Hist. Rev. 48, 143-163 (1974). doi: 10.2307/3112839
6. B. R. Glick, J. J. Pasternak, C. L. Patten, Eds., Molecular Biotechnology (American Society of Microbiology, 2010); http://www.asmscience.org/content/book/10.1128/9781555816124.
7. A. L. Demain, 01. History of Industrial Biotechnology (Wiley, 2010).
8. Energy Information Administration, Number and Capacity of Petroleum Refineries: Petroleum and Other Liquids (2016); https://www.eia.gov/dnav/pet/pet-pnp-cap1-dcu-nus-a.htm.
9. V. H. Dale et al., Incorporating bioenergy into sustainable landscape designs. Renew. Sustain. Energy Rev. 56, 1158-1171 (2016). doi: 10.1016/j.rser.2015.12.038
10. D. Zavala-Araiza, D. T. Allen, M. Harrison, F. C. George, G. R. Jersey, Allocating methane emissions to natural gas and oil production from shale formations. ACS Sustain. Chem.& Eng. 3, 492-498 (2015). doi: 10.1021/sc500730x
11. World Bank, World Development Indicators 2015 (World Bank, 2015); https://openknowledge.worldbank.org/handle/10986/21634.
12. E. Dahlgren, C. Göçmen, K. Lackner, G. van Ryzin, Small modular infrastructure. Eng. Econ. 58, 231-264 (2013). doi: 10.1080/0013791X.2013.825038
13. C. A. Haynes, R. Gonzalez, Rethinking biological activation of methane and conversion to liquid fuels. Nat. Chem. Biol. 10, 331-339 (2014). doi: 10.1038/nchembio.1509; pmid: 24743257
14. B. Erickson, P. Nelson, P. Winters, Perspective on opportunities in industrial biotechnology in renewable chemicals. Biotechnol. J. 7, 176-185 (2012). doi: 10.1002/biot.201100069; pmid: 21932250
15. J. D. Keasling, Manufacturing molecules through metabolic engineering. Science 330, 1355-1358 (2010). doi: 10.1126/science.1193990; pmid: 21127247
16. Energy Information Adminstration, Monthly Energy Review, Total Energy (EIA, 2016); http://www.eia.gov/totalenergy/data/monthly/index.cfm#renewable.
17. C. D. Elvidge, M. Zhizhin, F. C. Hsu, K. E. Baugh, VIIRS nightfire: Satellite pyrometry at night. Remote Sens. 5, 4423-4449 (2013). doi: 10.3390/rs5094423
18. R. Khalilpour, I. A. Karimi, in International Petroleum Technology Conference (IPTC, 2010), pp. 61-62.
19. S. Sirajuddin, A. C. Rosenzweig, Enzymatic oxidation of methane. Biochemistry 54, 2283-2294 (2015). doi: 10.1021/acs.biochem.5b00198; pmid: 25806595
20. M. G. Kalyuzhnaya, A. W. Puri, M. E. Lidstrom, Metabolic engineering in methanotrophic bacteria. Metab. Eng. 29, 142-152 (2015). doi: 10.1016/j.ymben.2015.03.010; pmid: 25825038
21. Y. A. Trotsenko, J. C. Murrell, Metabolic aspects of aerobic obligate methanotrophy. Adv. Appl. Microbiol. 63, 183-229 (2008). doi: 10.1016/S0065-2164(07)00005-6; pmid: 18395128
22. Q. Fei et al., Bioconversion of natural gas to liquid fuel: Opportunities and challenges. Biotechnol. Adv. 32, 596-614 (2014). doi: 10.1016/j.biotechadv.2014.03.011; pmid: 24726715
23. A. F. Khadem et al., Autotrophic methanotrophy in verrucomicrobia: Methylacidiphilum fumariolicum SolV uses the Calvin-Benson-Bassham cycle for carbon dioxide fixation. J. Bacteriol. 193, 4438-4446 (2011). doi: 10.1128/JB.00407-11; pmid: 21725016
24. A. Gilman et al., Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1. Microb. Cell Fact. 14, 182 (2015). doi: 10.1186/s12934-015-0372-8; pmid: 26572866
25. M. Kaluzhnaya et al., Taxonomic characterization of new alkaliphilic and alkalitolerant methanotrophs from soda lakes of the Southeastern Transbaikal region and description of Methylomicrobium buryatense sp.nov. Syst. Appl. Microbiol. 24, 166-176 (2001). doi: 10.1078/0723-2020-00028; pmid: 11518319
26. A. W. Puri et al., Genetic tools for the industrially promising methanotroph Methylomicrobium buryatense. Appl. Environ. Microbiol. 81, 1775-1781 (2015). doi: 10.1128/AEM.03795-14; pmid: 25548049
27. X. Yan, F. Chu, A. W. Puri, Y. Fu, M. E. Lidstrom, Electroporation-based genetic manipulation in type I methanotrophs. Appl. Environ. Microbiol. 82, 2062-2069 (2016). doi: 10.1128/AEM.03724-15; pmid: 26801578
28. C. A. Henard et al., Bioconversion of methane to lactate by an obligate methanotrophic bacterium. Sci. Rep. 6, 21585 (2016). doi: 10.1038/srep21585; pmid: 26902345
29. H. R. Christensen, L. C. Larsen, H. Frøkiaer, The oral immunogenicity of BioProtein, a bacterial single-cell protein, is affected by its particulate nature. Br. J. Nutr. 90, 169-178 (2003). doi: 10.1079/BJN2003863; pmid: 12844389
30. M. G. Kalyuzhnaya, A. W. Puri, M. E. Lidstrom, Metabolic engineering in methanotrophic bacteria. Metab. Eng. 29, 142-152 (2015). doi: 10.1016/j.ymben.2015.03.010; pmid: 25825038
31. P. Dürre, B. J. Eikmanns, C1-carbon sources for chemical and fuel production by microbial gas fermentation. Curr. Opin. Biotechnol. 35, 63-72 (2015). doi: 10.1016/j.copbio.2015.03.008; pmid: 25841103
32. A. J. Cal et al., Methanotrophic production of polyhydroxybutyrate-co-hydroxyvalerate with high hydroxyvalerate content. Int. J. Biol. Macromol. 87, 302-307 (2016). doi: 10.1016/j.ijbiomac.2016.02.056; pmid: 26920242
33. P. J. Strong et al., The opportunity for high-performance biomaterials from methane. Microorganisms 4, 11 (2016). doi: 10.3390/microorganisms4010011; pmid: 27681905
34. R. W. Ye et al., Construction of the astaxanthin biosynthetic pathway in a methanotrophic bacterium Methylomonas sp. strain 16a. J. Ind. Microbiol. Biotechnol. 34, 289-299 (2007). doi: 10.1007/s10295-006-0197-x; pmid: 17205350
35. E. G. Ivanova, D. N. Fedorov, N. V. Doronina, Y. A. Trotsenko, Production of vitamin B 12 in aerobic methylotrophic bacteria. Microbiology 75, 494-496 (2006). doi: 10.1134/S0026261706040217
36. X. Ge et al., High-rate, high-yield production of methanol by ammonia-oxidizing bacteria. Crit. Rev. Biochem. Mol. Biol. 212, 147-164 (2013).
37. M. G. Kalyuzhnaya et al., Highly efficient methane biocatalysis revealed in a methanotrophic bacterium. Nat. Commun. 4, 2785 (2013). doi: 10.1038/ncomms3785; pmid: 24302011
38. M. Kaluzhnaya et al., Taxonomic characterization of new alkaliphilic and alkalitolerant methanotrophs from soda lakes of the Southeastern Transbaikal region and description of Methylomicrobium buryatense sp.nov. Syst. Appl. Microbiol. 24, 166-176 (2001). doi: 10.1078/0723-2020-00028; pmid: 11518319
39. R. Balasubramanian et al., Oxidation of methane by a biological dicopper centre. Nature 465, 115-119 (2010). doi: 10.1038/nature08992; pmid: 20410881
40. A. de la Torre et al., Genome-scale metabolic reconstructions and theoretical investigation of methane conversion in Methylomicrobium buryatense strain 5G(B1). Microb. Cell Fact. 14, 188 (2015). doi: 10.1186/s12934-015-0377-3; pmid: 26607880
41. V. W. C. Soo et al., Reversing methanogenesis to capture methane for liquid biofuel precursors. Microb. Cell Fact. 15, 11 (2016). doi: 10.1186/s12934-015-0397-z; pmid: 26767617
42. S. Shima et al., Structure of a methyl-coenzyme M reductase from Black Sea mats that oxidize methane anaerobically. Nature 481, 98-101 (2011). doi: 10.1038/nature10663; pmid: 22121022
43. S. Scheller, M. Goenrich, R. Boecher, R. K. Thauer, B. Jaun, The key nickel enzyme of methanogenesis catalyses the anaerobic oxidation of methane. Nature 465, 606-608 (2010). doi: 10.1038/nature09015; pmid: 20520712
44. T. Wongnate et al., The radical mechanism of biological methane synthesis by methyl-coenzyme M reductase. Science 352, 953-958 (2016). doi: 10.1126/science.aaf0616; pmid: 27199421
45. K. Zheng, P. D. Ngo, V. L. Owens, X. P. Yang, S. O. Mansoorabadi, The biosynthetic pathway of coenzyme F430 in methanogenic and methanotrophic archaea. Science 354, 339-342 (2016). doi: 10.1126/science.aag2947; pmid: 27846569
46. S. Scheller, H. Yu, G. L. Chadwick, S. E. McGlynn, V. J. Orphan, Artificial electron acceptors decouple archaeal methane oxidation from sulfate reduction. Science 351, 703-707 (2016). doi: 10.1126/science.aad7154; pmid: 26912857
47. I. W. Bogorad et al., Building carbon-carbon bonds using a biocatalytic methanol condensation cycle. Proc. Natl. Acad. Sci. U.S.A. 111, 15928-15933 (2014). doi: 10.1073/pnas.1413470111; pmid: 25355907
48. J. E. N. Müller et al., Engineering Escherichia coli for methanol conversion. Metab. Eng. 28, 190-201 (2015). doi: 10.1016/j.ymben.2014.12.008; pmid: 25596507
49. J. B. Siegel et al., Computational protein design enables a novel one-carbon assimilation pathway. Proc. Natl. Acad. Sci. U.S.A. 112, 3704-3709 (2015). pmid: 25775555
50. D. C. Ducat, P. A. Silver, Improving carbon fixation pathways. Curr. Opin. Chem. Biol. 16, 337-344 (2012). doi: 10.1016/j.cbpa.2012.05.002; pmid: 22647231
51. S. A. Angermayr, A. Gorchs Rovira, K. J. Hellingwerf, Metabolic engineering of cyanobacteria for the synthesis of commodity products. Trends Biotechnol. 33, 352-361 (2015). doi: 10.1016/j.tibtech.2015.03.009; pmid: 25908503
52. H. Li et al., Integrated electromicrobial conversion of CO2 to higher alcohols. Science 335, 1596 (2012). doi: 10.1126/science.1217643; pmid: 22461604
53. J. P. Torella et al., Efficient solar-to-fuels production from a hybrid microbial-water-splitting catalyst system. Proc. Natl. Acad. Sci. U.S.A. 112, 2337-2342 (2015). doi: 10.1073/pnas.1424872112
54. M. W. Keller et al., Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide. Proc. Natl. Acad. Sci. U.S.A. 110, 5840-5845 (2013). doi: 10.1073/pnas.1222607110; pmid: 23530213
55. H. Latif, A. A. Zeidan, A. T. Nielsen, K. Zengler, Trash to treasure: Production of biofuels and commodity chemicals via syngas fermenting microorganisms. Curr. Opin. Biotechnol. 27, 79-87 (2014). doi: 10.1016/j.copbio.2013.12.001; pmid: 24863900
56. F. R. Bengelsdorf, M. Straub, P. Dürre, Bacterial synthesis gas (syngas) fermentation. Environ. Technol. 34, 1639-1651 (2013). doi: 10.1080/09593330.2013.827747; pmid: 24350425
57. B. Acharya, P. Roy, A. Dutta, Review of syngas fermentation processes for bioethanol. Biofuels 5, 551-564 (2014). doi: 10.1080/17597269.2014.1002996
58. M. Köpke et al., Clostridium ljungdahlii represents a microbial production platform based on syngas. Proc. Natl. Acad. Sci. U.S.A. 107, 13087-13092 (2010). doi: 10.1073/pnas.1004716107; pmid: 20616070
59. A. Banerjee, C. Leang, T. Ueki, K. P. Nevin, D. R. Lovley, Lactose-inducible system for metabolic engineering of Clostridium ljungdahlii. Appl. Environ. Microbiol. 80, 2410-2416 (2014). doi: 10.1128/AEM.03666-13; pmid: 24509933
60. A. Kita et al., Development of genetic transformation and heterologous expression system in carboxydotrophic thermophilic acetogen Moorella thermoacetica. J. Biosci. Bioeng. 115, 347-352 (2013). doi: 10.1016/j.jbiosc.2012.10.013; pmid: 23177215
61. T. Ueki, K. P. Nevin, T. L. Woodard, D. R. Lovley, Converting carbon dioxide to butyrate with an engineered strain of Clostridium ljungdahlii. MBio 5, e01636-14 (2014). doi: 10.1128/mBio.01636-14; pmid: 25336453
62. J. Mock et al., Energy conservation associated with ethanol formation from H2 and CO2 in Clostridium autoethanogenum involving electron bifurcation. J. Bacteriol. 197, 2965-2980 (2015). doi: 10.1128/JB.00399-15; pmid: 26148714
63. S. Hoffmeister et al., Acetone production with metabolically engineered strains of Acetobacterium woodii. Metab. Eng. 36, 37-47 (2016). doi: 10.1016/j.ymben.2016.03.001; pmid: 26971669
64. F. Liew et al., Gas fermentation: A flexible platform for commercial scale production of low-carbon-fuels and chemicals from waste and renewable feedstocks. Front. Microbiol. 7, 694 (2016). doi: 10.3389/fmicb.2016.00694; pmid: 27242719
65. K. P. Nevin, T. L. Woodard, A. E. Franks, Microbial electrosynthesis : Feeding microbes electricity to convert carbon dioxide and water to multicarbon extracellular organic. Am. Soc. Microbiol. 1, 1-4 (2010). pmid: 20714445
66. C. Leang, T. Ueki, K. P. Nevin, D. R. Lovley, A genetic system for Clostridium ljungdahlii: A chassis for autotrophic production of biocommodities and a model homoacetogen. Appl. Environ. Microbiol. 79, 1102-1109 (2013). doi: 10.1128/AEM.02891-12; pmid: 23204413
67. K. K. Sakimoto, A. B. Wong, P. Yang, Self-photosensitization of nonphotosynthetic bacteria for solar-to-chemical production. Science 351, 74-77 (2016). doi: 10.1126/science. aad3317; pmid: 26721997
68. K. Schuchmann, V. Müller, Direct and reversible hydrogenation of CO2 to formate by a bacterial carbon dioxide reductase. Science 342, 1382-1385 (2013). doi: 10.1126/science.1244758; pmid: 24337298
69. K. Schuchmann, V. Müller, Autotrophy at the thermodynamic limit of life: A model for energy conservation in acetogenic bacteria. Nat. Rev. Microbiol. 12, 809-821 (2014). doi: 10.1038/nrmicro3365; pmid: 25383604
70. L. B. Maia, L. Fonseca, I. Moura, J. J. G. Moura, Reduction of carbon dioxide by a molybdenum-containing formate dehydrogenase: A kinetic and mechanistic study. J. Am. Chem. Soc. 138, 8834-8846 (2016). doi: 10.1021/jacs.6b03941; pmid: 27348246
71. C. Y. Ng, A. Khodayari, A. Chowdhury, C. D. Maranas, Advances in de novo strain design using integrated systems and synthetic biology tools. Curr. Opin. Chem. Biol. 28, 105-114 (2015). doi: 10.1016/j.cbpa.2015.06.026; pmid: 26177080
72. N. Ziemert, M. Alanjary, T. Weber, The evolution of genome mining in microbes-A review. Nat. Prod. Rep. 33, 988-1005 (2016). doi: 10.1039/C6NP00025H; pmid: 27272205
73. W. S. Mak, J. B. Siegel, Computational enzyme design: Transitioning from catalytic proteins to enzymes. Curr. Opin. Struct. Biol. 27, 87-94 (2014). doi: 10.1016/j.sbi.2014.05.010; pmid: 25005925
74. I. Wheeldon et al., Substrate channelling as an approach to cascade reactions. Nat. Chem. 8, 299-309 (2016). doi: 10.1038/nchem.2459; pmid: 27001725
75. X. Jia et al., Design, analysis and application of synthetic microbial consortia. Synth. Syst. Biotechnol. 1, 109-117 (2016). doi: 10.1016/j.synbio.2016.02.001
76. H. Zhang, X. Wang, Modular co-culture engineering, a new approach for metabolic engineering. Metab. Eng. 37, 114-121 (2016). doi: 10.1016/j.ymben.2016.05.007; pmid: 27242132
77. A. S. Karim, M. C. Jewett, A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery. Metab. Eng. 36, 116-126 (2016). doi: 10.1016/j.ymben.2016.03.002; pmid: 26996382
78. C. D. Blanchette et al., Printable enzyme-embedded materials for methane to methanol conversion. Nat. Commun. 7, 11900 (2016). doi: 10.1038/ncomms11900; pmid: 27301270
79. W. Jiang, D. Bikard, D. Cox, F. Zhang, L. A. Marraffini, RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat. Biotechnol. 31, 233-239 (2013). doi: 10.1038/nbt.2508; pmid: 23360965
80. J. E. DiCarlo et al., Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res. 41, 4336-4343 (2013). doi: 10.1093/nar/gkt135; pmid: 23460208
81. T. Jakočiunas et al., Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae. Metab. Eng. 28, 213-222 (2015). doi: 10.1016/j.ymben.2015.01.008; pmid: 25638686
82. H. H. Wang et al., Programming cells by multiplex genome engineering and accelerated evolution. Nature 460, 894-898 (2009). doi: 10.1038/nature08187; pmid: 19633652
83. C. A. Hutchison 3rd et al., Design and synthesis of a minimal bacterial genome. Science 351, aad6253 (2016). doi: 10.1126/science.aad6253; pmid: 27013737
84. J. K. Rogers, N. D. Taylor, G. M. Church, Biosensor-based engineering of biosynthetic pathways. Curr. Opin. Biotechnol. 42, 84-91 (2016). doi: 10.1016/j.copbio.2016.03.005; pmid: 26998575
85. T. C. Williams, I. S. Pretorius, I. T. Paulsen, Synthetic evolution of metabolic productivity using biosensors. Trends Biotechnol. 34, 371-381 (2016). doi: 10.1016/j.tibtech.2016.02.002; pmid: 26948437
86. V. Chubukov, A. Mukhopadhyay, C. Petzold, J. Keasling, H. G. Martín, Synthetic and systems biology for microbial production of commodity chemicals. npj Syst. Biol. Appl. 2, 16009 (2016). doi: 10.1038/npjsba.2016.9
87. P. Neubauer et al., Consistent development of bioprocesses from microliter cultures to the industrial scale. Eng. Life Sci. 13, 224-238 (2013). doi: 10.1002/elsc.201200021
88. A. A. Menezes, J. Cumbers, J. A. Hogan, A. P. Arkin, Towards synthetic biological approaches to resource utilization on space missions. J. R. Soc. Interface 12, 20140715-20140715 (2015). doi: 10.1098/rsif.2014.0715; pmid: 25376875
89. A. A. Menezes, M. G. Montague, J. Cumbers, J. A. Hogan, A. P. Arkin, Grand challenges in space synthetic biology. J. R. Soc. Interface 12, 20150803 (2015). doi: 10.1098/rsif.2015.0803; pmid: 26631337
90. T. Brown, Ammonia Plants in North America. Google Fusion Table (2013); https://fusiontables.google.com/data?docid= 1vXUF9q5X0vbWID-JAzpxaByp28lwlr3gs0y2zg8#map:id=3.
91. CF Industries, Donaldsonville Nitrogen Facility (2016); https://www.cfindustries.com/who-we-are/locations/donaldsonville-nitrogen-facility.
92. Yara, World scale ammonia plant opens in Burrup (2006); http://yara.com/media/press-releases/1045462/press-release/200604/world-scale-ammonia-plant-opens-in-burrup/.
93. G. Morgan, The Sturgeon refinery and the high cost of valueadded. Alberta Oil (2014); http://www.albertaoilmagazine. com/2014/11/high-price-adding-value/.
94. E. O'Brien, Gas to liquids plants: Turning Louisiana natural gas into marketable liquid fuels (Louisiana Department of Natural Resources, Technology Assessment Division, 2013).
95. J. Patton, Incitec Plans $850 Million U.S. Ammonia Plant on Gas Price. Bloomberg (2013); http://www.bloomberg.com/news/articles/2013-04-17/incitec-plans-850-million-u-sammonia-plant-on-gas-price.
96. U. S. Energy Information Administration, Ethanol Plants. Layer Information for Interactive State Maps (2016); https://www.eia.gov/maps/layer-info-m.cfm.
97. U.S. Energy Information Administration, Petroleum Refineries. Layer Information for Interactive State Maps (2016); https://www.eia.gov/maps/layer-info-m.cfm.
98. I. Foris, I. F. Announces, $265 million ammonia plant in Grant Parish. Louisiana Econ. Dev. (2015); http://www. opportunitylouisiana.com/led-news/news-releases/news/2015/07/22/investimus-foris-announces-$265-millionammonia-plant-in-grant-parish.
99. Google Maps, Big River United Energy (2016); https://www. google.com/maps/place/Big+River+United+Energy/@ 42.4892966,-91.1562547,17z/data=!3m1!4b1!4m5!3m4! 1s0x87e3546c5bd01ea9:0xc8a545926175dc77!8m2! 3d42.4892966!4d-91.1540607.
100. G. Maps, ExxonMobil Baytown Refinery (2016); https://www. google.com/maps/place/Exxonmobil+Refinery/@ 29.7562611,-94.9913522,15z/data=!4m2!3m1! 1s0x0:0x1700615ea16f9969?sa=X&ved=0ahUKEwi-5qaFqOfOAhVDMyYKHeBICM0Q-BIIeDAK.
101. N. Jessen, DuPont: Collaborations key in building 30 MMgy cellulosic plant (2012); http://www.ethanolproducer.com/articles/9338/dupont-collaborations-key-in-building-30-mmgy-cellulosic-plant.
102. Tate, DuPont, 1,3-propanediol FAQs (2016); http://www. duponttateandlyle.com/faqs.
103. Genomatica, Novamont opens world's first commercial plant for bio-production of a major intermediate chemical (2016); http://www.genomatica.com/news/press-releases/Novamont-opensworlds-first-commercial-plant-for-bio-based-intermediate/.
104. W. G. Hettinga et al., Understanding the reductions in US corn ethanol production costs: An experience curve approach. Energy Policy 1, 190-203 (2009). doi: 10.1016/j.enpol.2008.08.002
105. H. L. David, G. S. Hammaker, R. J. Buzenberg, J. P. Wagner, Gasohol Economic Feasibility Study (Oxford Univ. Press, 1978).
106. M. LeBlanc, A. Prato, Ethanol production from grain in the United States: Agricultural impacts and economic feasibility. Can. J. Agric. Econ. 31, 223-232 (1983). doi: 10.1111/j.1744-7976.1983. tb01198.x
107. U.S. Department of Agriculture, Fuel Ethanol and Agriculture: An Economic Asessment (USDA, 1986).
108. J. Whims, "Corn based ethanol costs and margins: Attachment 1" (Sparks Companies Inc. and Kansas State University, 2002); www.agmrc.org/media/cms/ksueth1-44EF748246D5F.pdf.
109. R. Dale, W. Tyner, Economic and technical analysis of ethanol dry milling: Model description (Purdue University, 2006); http://www.researchgate.net/publication/5218817-Economic-And-Technical-Analysis-Of-Ethanol-Dry-Milling-Model-Description/file/79e4150eec5e1635d6.pdf.
110. J. R. Kwiatkowski, A. J. McAloon, F. Taylor, D. B. Johnston, Modeling the process and costs of fuel ethanol production by the corn dry-grind process. Ind. Crops Prod. 23, 288-296 (2006). doi: 10.1016/j.indcrop.2005.08.004
111. J. Coombe, Feasibility study for small-scale ethanol production in Minnesota (BBI International, 2009).
112. T. Sato, H. Atomi, Microbial inorganic carbon fixation. eLS, 1-12 (2001); http://www.els.net/WileyCDA/ElsArticle/refIda0021900. html.
113. J. C. Liao, L. Mi, S. Pontrelli, S. Luo, Fuelling the future: Microbial engineering for the production of sustainable biofuels. Nat. Rev. Microbiol. 14, 288-304 (2016). doi: 10.1038/nrmicro.2016.32; pmid: 27026253
114. G. Michal, Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (Wiley, New York, ed. 1, 1999; http://www.amazon.co.uk/Biochemical-Pathways-Biochemistry-Molecular-Biology/dp/0470146842/ref=sr-1-1? s=books&ie=UTF8&qid=1447518627&sr=1-1&keywords=biochemical+pathways).
115. A. Flamholz, E. Noor, A. Bar-Even, W. Liebermeister, R. Milo, Glycolytic strategy as a tradeoff between energy yield and protein cost. Proc. Natl. Acad. Sci. U.S.A. 110, 10039-10044 (2013). doi: 10.1073/pnas.1215283110; pmid: 23630264
116. K. H. Rostkowski, A. R. Pfluger, C. S. Criddle, Stoichiometry and kinetics of the PHB-producing Type II methanotrophs Methylosinus trichosporium OB3b and Methylocystis parvus OBBP. Bioresour. Technol. 132, 71-77 (2013). doi: 10.1016/j.biortech.2012.12.129; pmid: 23395757
117. C. Duan, M. Luo, X. Xing, High-rate conversion of methane to methanol by Methylosinus trichosporium OB3b. Bioresour. Technol. 102, 7349-7353 (2011). doi: 10.1016/j.biortech.2011.04.096; pmid: 21612919
118. T. Hölscher, U. Breuer, L. Adrian, H. Harms, T. Maskow, Production of the chiral compound (R)-3-hydroxybutyrate by a genetically engineered methylotrophic bacterium. Appl. Environ. Microbiol. 76, 5585-5591 (2010). doi: 10.1128/AEM.01065-10; pmid: 20581197
119. T. M. B. Heggeset et al., Genome sequence of thermotolerant Bacillus methanolicus: Features and regulation related to methylotrophy and production of L-lysine and L-glutamate from methanol. Appl. Environ. Microbiol. 78, 5170-5181 (2012). doi: 10.1128/AEM.00703-12; pmid: 22610424
120. T. Brautaset et al., Bacillus methanolicus pyruvate carboxylase and homoserine dehydrogenase I and II and their roles for L-lysine production from methanol at 50 degrees C. Appl. Microbiol. Biotechnol. 87, 951-964 (2010). doi: 10.1007/s00253-010-2559-6; pmid: 20372887
121. I. Naerdal, J. Pfeifenschneider, T. Brautaset, V. F. Wendisch, Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains. Microb. Biotechnol. 8, 342-350 (2015). doi: 10.1111/1751-7915.12257 pmid: 25644214
122. F. Sonntag et al., Engineering Methylobacterium extorquens for de novo synthesis of the sesquiterpenoid a-humulene from methanol. Metab. Eng. 32, 82-94 (2015). doi: 10.1016/j.ymben.2015.09.004; pmid: 26369439