Development of an insertional expression vector system for Methylobacterium extorquens AM1 and generation of null mutants lacking mtdA and/or fch

Microbiology

Volumn 150, Issue 1, 2004, Pages 9-19

  Marx, Christopher J ^a,c   Lidstrom, Mary E ^a,b  

^a UNIVERSITY OF WASHINGTON   (United States)

^b University of Washington   (United States)

^c MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

METHENYLTETRAHYDROFOLATE CYCLOHYDROLASE; METHYLENETETRAHYDROFOLATE DEHYDROGENASE; METHYLENETETRAHYDROFOLIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; CONTROLLED STUDY; EXPRESSION VECTOR; GENE EXPRESSION; GENE INSERTION; GENE LOCUS; GENE MUTATION; GENETIC CODE; METHYLOBACTERIUM; METHYLOBACTERIUM CHLOROMETHANICUM; METHYLOBACTERIUM EXTORQUENS; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); METHYLOBACTERIUM; METHYLOBACTERIUM CHLOROMETHANICUM; METHYLOBACTERIUM EXTORQUENS; NEGIBACTERIA; PROKARYOTA;

EID: 1642555532     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.26587-0     Document Type: Article

References (36)

1. Attwood, M. M. & Harder, W. (1972). A rapid and specific enrichment procedure for Hyphomicrobium spp. Antonie Van Leeuwenhoek 38, 369-377.
2. Chambers, S. P., Prior, S. E., Barstow, D. A. & Minton, N. P. (1988). The pMTL nic cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing. Gene 68, 139-149.
3. Chistoserdov, A. Y., Chistoserdova, L. V., McIntire, W. S. & Lidstrom, M. E. (1994). Genetic organization of the mau gene cluster in Methylobacterium extorquens AM1: complete nucleotide sequence and generation and characteristics of mau mutants. J Bacteriol 176, 4052-4065.
4. 1 transfer enzymes and coenzymes linking methylotrophic bacteria and methanogenic Archaea. Science 281, 99-102.
5. Chistoserdova, L., Chen, S. W., Lapidus, A. & Lidstrom, M. E. (2003). Methylotrophy in Methylobacterium extorquens AM1 from a genomic point of view. J Bacteriol 185, 2980-2987.
6. Chistoserdova, L. V. & Lidstrom, M. E. (1994a). Genetics of the serine cycle in Methylobacterium extorquens AM1: identification, sequence, and mutation of three new genes involved in C1 assimilation, orf4, mtkA, and mtkB. J Bacteriol 176, 7398-7404.
7. Chistoserdova, L. V. & Lidstrom, M. E. (1994b). Genetics of the serine cycle in Methylobacterium extorquens AM1: identification of sgaA and mtdA and sequences of sgaA, hprA, and mtdA. J Bacteriol 176, 1957-1968.
8. Chistoserdova, L. V. & Lidstrom, M. E. (1996). Molecular characterization of a chromosomal region involved in the oxidation of acetyl-CoA to glyoxylate in the isocitrate-lyase-negative methylotroph Methylobacterium extorquens AM1. Microbiology 142, 1459-1468.
9. Figurski, D. H. & Helinski, D. R. (1979). Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci U S A 76, 1648-1652.
10. Hagemeier, C. H., Chistoserdova, L., Lidstrom, M. E., Thauer, R. K. & Vorholt, J. A. (2000). Characterization of a second methylene tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1. Eur J Biochem 267, 3762-3769.
11. Kalb, V. F. & Bernlohr, R. W. (1977). A new spectrophotometric assay for protein in cell extracts. Anal Biochem 82, 362-371.
12. Kataeva, I. M. & Golovleva, L. A. (1990). Catechol 2,3-dioxygenases from Pseudomonas aeruginosa 2x. Methods Enzymol 188, 115-121.
13. 1 compounds. 2. Synthesis of cell constituents by methanol-and formate-grown Pseudomonas AM1, and methanol-grown Hyphomicrobium vulgare. Biochem J 81, 470-479.
14. Laukel, M., Chistoserdova, L., Lidstrom, M. E. & Vorholt, J. A. (2003). The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: purification and properties. Eur J Biochem 270, 325-333.
15. Lidstrom, M. E. (2001). Aerobic methylotrophic prokaryotes. In The Prokaryotes, posting date 2 November, 2001 (http://141.150.157.117:8080/prokPUB/chaprender/jsp/showchap.jsp?chapnum= 300&initsec=01_00). Edited by M. Dworkin.
16. Marison, I. W. & Attwood, M. M. (1982). A possible alternative mechanism for the oxidation of formaldehyde to formate. J Gen Microbiol 128, 1441-1446.
17. Marx, C. J. & Lidstrom, M. E. (2001). Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria. Microbiology 147, 2065-2075.
18. Marx, C. J. & Lidstrom, M. E. (2002). Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria. BioTechniques 33, 1062-1067.
19. Marx, C. J., Laukel, M., Vorholt, J. A. & Lidstrom, M. E. (2003a). Purification of the formate-tetrahydrofolate ligase from Methylobacterium extorquens AM1 and demonstration of its requirement for methylotrophic growth. J Bacteriol 185, 7169-7175.
20. Marx, C. J., O'Brien, B. N., Breezee, J. & Lidstrom, M. E. (2003b). Novel methylotrophy genes of Methylobacterium extorquens AM1 identified by using transposon mutagenesis including a putative dihydromethanopterin reductase. J Bacteriol 185, 669-673.
21. Nagy, P. L., Marolewski, A., Benkovic, S. J. & Zalkin, H. (1995). Formyltetrahydrofolate hydrolase: a regulatory enzyme that functions to balance pools of tetrahydrofolate and one-carbon tetrahydrofolate adducts in Escherichia coli. J Bacteriol 177, 1292-1298.
22. Nunn, D. N. & Lidstrom, M. E. (1986). Isolation and complementation analysis of 10 methanol oxidation mutant classes and identification of the methanol dehydrogenase structural gene of Methylobacterium sp. strain AM1. J Bacteriol 166, 581-590.
23. Palmeros, B., Wild, J., Szybalski, W., Le Borgne, S., Hernandez-Chavez, G., Gosset, G., Valle, F. & Bolivar, F. (2000). A family of removable cassettes designed to obtain antibiotic-resistance-free genomic modifications of Escherichia coli and other bacteria. Gene 247, 255-264.
24. Pomper, B. K. & Vorholt, J. A. (2001). Characterization of the formyltransferase from Methylobacterium extorquens AM1. Eur J Biochem 268, 4769-4775.
25. Pomper, B. K., Vorholt, J. A., Chistoserdova, L., Lidstrom, M. E. & Thauer, R. K. (1999). A methenyl tetrahydromethanopterin cyclohydrolase and a methenyl tetrahydrofolate cyclohydrolase in Methylobacterium extorquens AM1. Eur J Biochem 261, 475-480.
26. Pomper, B. K., Saurel, O., Milon, A. & Vorholt, J. A. (2002). Generation of formate by the formyltransferase/hydrolase complex (Fhc) from Methylobacterium extorquens AM1. FEBS Lett 523, 133-137.
27. Purdy, D., O'Keeffe, T. A., Elmore, M., Herbert, M., McLeod, A., Bokori-Brown, M., Ostrowski, A. & Minton, N. P. (2002). Conjugative transfer of clostridial shuttle vectors from Escherichia coli to Clostridium difficile through circumvention of the restriction barrier. Mol Microbiol 46, 439-452.
28. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
29. Simon, R., Priefer, U. & Puhler, A. (1983). A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio/Technology 1, 784-791.
30. 1 utilization pathway in Methylobacterium chloromethanicum CM4. J Bacteriol 184, 3476-3484.
31. Toyama, H., Anthony, C. & Lidstrom, M. E. (1998). Construction of insertion and deletion mxa mutants of Methylobacterium extorquens AM1 by electroporation. FEMS Microbiol Lett 166, 1-7.
32. Vannelli, T., Messmer, M., Studer, A., Vuilleumier, S. & Leisinger, T. (1999). A corrinoid-dependent catabolic pathway for growth of a Methylobacterium strain with chloromethane. Proc Natl Acad Sci U S A 96, 4615-4620.
33. Vorholt, J. A. (2002). Cofactor-dependent pathways of formaldehyde oxidation in methylotrophic bacteria. Arch Microbiol 178, 239-249.
34. Vorholt, J. A., Chistoserdova, L., Lidstrom, M. E. & Thauer, R. K. (1998). The NADP-dependent methylene tetrahydromethanopterin dehydrogenase in Methylobacterium extorquens AM1. J Bacteriol 180, 5351-5356.
35. Vorholt, J. A., Marx, C. J., Lidstrom, M. E. & Thauer, R. K. (2000). Novel formaldehyde-activating enzyme in Methylobacterium extorquens AM1 required for growth on methanol. J Bacteriol 182, 6645-6650.
36. Whitaker, J. R. & Granum, P. E. (1980). An absolute method for protein determination based on the difference at 235 and 280 nm. Anal Biochem 109, 156-159.