Characterization and evolution of an activator-independent methanol dehydrogenase from Cupriavidus necator N-1

Applied Microbiology and Biotechnology

Volumn 100, Issue 11, 2016, Pages 4969-4983

  Wu, Tung Yun ^a   Chen, Chang Ting ^a   Liu, Jessica Tse Jin ^a   Bogorad, Igor W ^a   Damoiseaux, Robert ^b   Liao, James C ^a  

^a Engineering IV   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Directed molecular evolution; High throughput screening; Methanol dehydrogenase; Methanol utilization

Indexed keywords

BACTERIOLOGY; CHEMICAL ACTIVATION; ENZYMES; ESCHERICHIA COLI; MOLECULAR BIOLOGY;

ALCOHOL DEHYDROGENASE; BACILLUS METHANOLICUS; CATALYTIC EFFICIENCIES; ENDOGENOUS ACTIVATOR; HIGH THROUGHPUT SCREENING; METHANOL DEHYDROGENASE; METHANOL UTILIZATION; MOLECULAR EVOLUTION;

METHANOL;

ACETYLACETONE; ALCOHOL; ALCOHOL DEHYDROGENASE; AMMONIUM ACETATE; ASPARTIC ACID; BUTANOL; HISTIDINE; METHANOL; METHANOL DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; NUDIX HYDROLASE; PROPANOL; ALCOHOL DEHYDROGENASE (ACCEPTOR); BACTERIAL PROTEIN; INORGANIC PYROPHOSPHATASE; NUDIX HYDROLASES;

BACTERIUM; CATALYSIS; ELECTRON; ENZYME ACTIVITY; GENETIC ANALYSIS; METHANOL; OXIDATION; PROTEIN; TEMPERATURE GRADIENT;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CATALYTIC EFFICIENCY; CELL ASSAY; CELL DENSITY; COMPARATIVE STUDY; CONTROLLED STUDY; CUPRIAVIDUS NECATOR; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; HIGH THROUGHPUT SCREENING; KINETIC PARAMETERS; MOLECULAR WEIGHT; NONHUMAN; OXIDATION; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS; TEMPERATURE SENSITIVITY; THERMOSTABILITY; BACILLUS; BIOTRANSFORMATION; CATALYSIS; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR EVOLUTION; TEMPERATURE;

BACILLUS METHANOLICUS; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI; NEGIBACTERIA;

1-BUTANOL; ALCOHOL OXIDOREDUCTASES; AMINO ACID SEQUENCE; BACILLUS; BACTERIAL PROTEINS; BIOTRANSFORMATION; CATALYSIS; CLONING, MOLECULAR; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI; EVOLUTION, MOLECULAR; GENE EXPRESSION REGULATION, BACTERIAL; HYDROGEN-ION CONCENTRATION; METHANOL; NAD; PYROPHOSPHATASES; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84957595673     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-016-7320-3     Document Type: Article

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