Antioxidants for male subfertility

Cochrane Database of Systematic Reviews

Volumn 2019, Issue 3, 2019, Pages

  Smits, Roos M ^a   Mackenzie Proctor, Rebecca ^b   Yazdani, Anusch ^c   Stankiewicz, Marcin T ^d   Jordan, Vanessa ^e   Showell, Marian G ^e  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

^b AUCKLAND CITY HOSPITAL   (New Zealand)

^c Queensland Fertility Group   (Australia)

^d Ashford Specialist Centre Suite 22 ^*  (Australia)

^e UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCYSTEINE; ALPHA TOCOPHEROL; ANTIOXIDANT; CARNITINE; DOCOSAHEXAENOIC ACID; ETHYL CYSTEINE; FOLIC ACID; LEVACECARNINE; SELENIUM; UNCLASSIFIED DRUG; ZINC;

ADVERSE EVENT; DIET SUPPLEMENTATION; GASTROINTESTINAL SYMPTOM; HUMAN; IN VITRO FERTILIZATION; INTRACYTOPLASMIC SPERM INJECTION; LIVE BIRTH; MALE SUBFERTILITY; MEN'S HEALTH; META ANALYSIS; OUTCOME ASSESSMENT; PREGNANCY; PREGNANCY RATE; PRIORITY JOURNAL; RANDOMIZED CONTROLLED TRIAL (TOPIC); REVIEW; RISK ASSESSMENT; SEMEN ANALYSIS; SPERMATOZOON MOTILITY; SPONTANEOUS ABORTION; SUBFERTILITY; SYSTEMATIC REVIEW; CHEMICALLY INDUCED; DNA DAMAGE; DNA FRAGMENTATION; DRUG EFFECT; FEMALE; GASTROINTESTINAL DISEASE; MALE; MALE INFERTILITY; OXIDATIVE STRESS; SPERMATOZOON; SPERMATOZOON COUNT;

ABORTION, SPONTANEOUS; ANTIOXIDANTS; DNA DAMAGE; DNA FRAGMENTATION; FEMALE; GASTROINTESTINAL DISEASES; HUMANS; INFERTILITY, MALE; LIVE BIRTH; MALE; OXIDATIVE STRESS; PREGNANCY; PREGNANCY RATE; RANDOMIZED CONTROLLED TRIALS AS TOPIC; SPERM COUNT; SPERM MOTILITY; SPERMATOZOA;

EID: 85062936204     PISSN: None     EISSN: 1469493X     Source Type: Journal    
DOI: 10.1002/14651858.CD007411.pub4     Document Type: Review

References (283)

1. Akiyama M. In vivo scavenging effect of ethylcysteine on reactive oxygen species in human semen. Japanese Journal of Urology 1999;90(3):421-8.
2. Attallah D, El Nashar IH, Mahmoud R, Shaaban OM, Salman SA. N-acytelcysteine prior to intrauterine insemination in couples with isolated athenozospermia: a randomized controlled trial. Fertility and Sterility 2013;100 (3 Suppl):S462.
3. Azizollahi G, Azizollahi S, Babaei H, Kianinejad M, Baneshi MR, Nematollahi-mahani SN. Effects of supplement therapy on sperm parameters, protamine content and acrosomal integrity of varicocelectomized subjects. Journal of Assisted Reproduction and Genetics 2013; 30(4):593-9.
4. Azizollahi G, Azizollahi S, Babaei H, Kianinejad MA, Baneshi MR, Nematollahi-Mahani SN. Effects of zinc sulfate and folic acid coadministration on sperm parameters, protamine content and acrosomal integrity of varicocelectomized patients. Iranian Journal of Reproductive Medicine 2013;1):37.
5. Azizollahi S, Azizollahi G, Nematollahi SN, Babaei H, Rastegari A, Maghsudi S. The effect of folic acid administration on protamine deficiency, acrosomal activity and semen parameters in varicocelectomized patients. Human Reproduction 2011;26 Suppl 1 Abstract no: P-57: i340.
6. Azizollahi S, Nematollahi-Mahani SN, Azizollahi G, Baneshi MR, Safari Z. The effect of folic acid and zinc sulfate on endocrine parameters and seminal antioxidant level, after variocelectomy. Iranian Journal of Reproductive Medicine 2013;1):36.
7. Balercia G, Regoli F, Armeni T, Koverech A, Mantero F, Boscaro M. Placebo-controlled double-blind randomized trial on the use of L-carnitine, L-acetylcarnitine, or combined L-carnitine and L-acetylcarnitine in men with idiopathic asthenozoospermia. Fertility and Sterility 2005; 84(3):662-71.
8. Balercia G, Buldreghini E, Vignini A, Tiano L, Paggi F, Amoroso S, et al. Coenzyme Q10 treatment in infertile men with idiopathic asthenozoospermia: a placebo-controlled, double-blind randomized trial. Fertility and Sterility 2009; 91(5):1785-92.
9. Barekat F, Tavalaee M, Deemeh MR, Bahreinian M, Azadi L, Abbasi H, et al. A Preliminary Study: N-acetyl-L-cysteine Improves Semen Quality following Varicocelectomy. International Journal of Fertility & Sterility 2016;10(1): 120-6.
10. Biagiotti G, Cavallini G, Modenini F, Vitali G, Magli C, Ferraretti A. Prostaglanadins pulsed down-regulation enhances carnitine therapy performance in severe idiopathic oligoasthenospermia. Human Reproduction 2003;18 Suppl 1:202.
11. Blomberg Jensen M, Gerner Lawaetz J, Andersson A, Petersen JH, Nordkap L, Bang AK, et al. Vitamin D deficiency and low ionized calcium are linked with semen quality and sex steroid levels in infertile men. Human Reproduction 2016;31(8):1875-85.
12. Blomberg Jensen M, Lawaetz JG, Petersen JH, Juul A, Jorgensen N. Effects of vitamin D supplementation on semen quality, reproductive hormones, and live birth rate: a randomized clinical trial. Journal of Clinical Endocrinology & Metabolism 2018;103(3):870-81.
13. Boonyarangkul A, Vinayanuvattikhun N, Chiamchanya C, Visutakul P. Comparative study of the effects of tamoxifen citrate and folate on semen quality of the infertile male with semen abnormality. Journal of the Medical Association of Thailand 2015;98(11):1057-63.
14. Busetto G, Virmani A, Del Giudice F, Micic S, Agarwal A, De Berardinis E. Varicocele and oligoasthenoteratozoospermia: Evaluation of antioxidant supplementation effect on pregnancy rate and sperm quality. Fertility and Sterility 2017;108 (3 Supplement 1):e133.
15. Busetto G, Virmani MA, Antonini G, Ragonesi G, De Berardinis E, Agarwal A, et al. Effect of antioxidant supplementation on sperm parameters in oligoasthenoteratozoospermia, with and without varicocele: A DBPC study. Fertility and Sterility 2016;106 (Supplement 3):e46.
16. Busetto GM, Agarwal A, Virmani A, Antonini G, Ragonesi G, Del Giudice F, et al. Effect of metabolic and antioxidant supplementation on sperm parameters in oligo-astheno-teratozoospermia, with and without varicocele: a double-blind placebo-controlled study. Andrologia 2018;07: 07.
17. Busetto GM, Virmani A, Antonini G, Ragonesi G, Del Giudice F, Agarwal A, et al. Effect of antioxidant supplementation on sperm parameters in oligo-astheno-teratozoospermia, with and without varicocele: A double blind place controlled (DBPC) study. Human Reproduction 2017;32 (Supplement 1):i142-3.
18. Cavallini G, Ferraretti AP, Gianaroli L, Biagiotti G, Vitali G. Cinnoxicam and L-carnitine/acetyl-L-carnitine treatment for idiopathic and varicocoele associated oligoasthenospermia [see comment]. Journal of Andrology 2004; Vol. 25, issue 5:761-70.
19. Cavallini G, Ferraretti AP, Gianaroli L, Magli MC, Biagiotti G, Vitali G. Cinnoxicam + carnitines for idiopathic dyspermia. The 20th Annual Meeting of the European Society of Human Reproduction and Embryology. Human Reproduction 2004:i23-4.
20. Conquer JA, Martin JB, Tummon I, Watson L, Tekpetey F. Effect of DHA supplementation on DHA status and sperm motility in asthenozoospermic males. Lipids 2000;35(2): 149-54.
21. Cyrus A, Kabir A, Goodarzi D, Moghimi M. The effect of adjuvant vitamin C after varicocele surgery on sperm quality and quantity in infertile men: a double blind placebo controlled clinical trial. International Brazilian Journal of Urology 2015; Vol. 41, issue 2:230-8.
22. Dawson EB, Harris WA, Powell LC. Relationship between ascorbic acid and male fertility. World Review of Nutrition and Dietetics 1990; Vol. 62:1-26.
23. Deng XL, Li YM, Yang XY, Huang JR, Guo SL, Song LM. Efficacy and safety of vitamin D in the treatment of idiopathic oligoasthenozoospermia. [Chinese]. Zhonghua nan ke xue = National Journal of Andrology 2014; Vol. 20, issue 12:1082-5.
24. Deng XL, Li YM, Yang XY, Huang JR, Guo SL, Song LM. [Efficacy and safety of vitamin D in the treatment of idiopathic oligoasthenozoospermia]. Zhong Hua Nan Ke Xue 2014; Vol. 20, issue 12:1082-5.
25. Dimitriadis F, Tsambalas S, Tsounapi P, Kawamura H, Vlachopoulou E, Haliasos N, et al. Effects of phosphodiesterase-5 inhibitors on Leydig cell secretory function in oligoasthenospermic infertile men: A randomized trial. BJU International 2010;106(8):1181-5.
26. Ener K, Aldemir M, Isik E, Okulu E, Ozcan MF, Ugurlu M, et al. The impact of vitamin E supplementation on semen parameters and pregnancy rates after varicocelectomy: A randomised controlled study. Andrologia 2016;48(7): 829-34.
27. Eslamian G, Amirjannati N, Sadeghi MR, Rashidkhani B, Pahlavan S, Hooshangi A, et al. The effects of combined supplementation of docosahexaenoic acid and vitamin E on fatty acid changes in sperm membrane in asthenozoospermic men. Iranian Journal of Nutrition Sciences & Food Technology 2013;8(1):23-37.
28. Hekmatdoost A. The effects of administration of combined docosahexaenoic acid and vitamin E supplements on spermatogram and seminal plasma oxidative stress in infertile men with asthenozoospermia. www.clinicaltrials.gov.ClinicalTrials.gov: NCT01846325]
29. Exposito A, Perez-Sanz J, Crisol L, Aspichueta F, Quevedo S, Diaz-Nunez M, et al. A prospective double-blind randomized placebo-controlled study of the effect of vitamin E on semen parameters in infertile men. Human Reproduction 2016; Vol. 31 (Supplement 1):i137-8.
30. Exposito A, Perez-Sanz J, Crisol L, Aspichueta F, Quevedo S, Diaz-Nunez M, et al. A prospective double-blind randomized placebo-controlled study of the effect of vitamin E on semen parameters in infetile men. Human Reproduction 2016; Vol. 31 (Supp1):i137-i138 Abstract no: P-018.
31. Galatioto GP, Gravina GL, Angelozzi G, Sacchetti A, Innominato PF, Pace G, et al. May antioxidant therapy improve sperm parameters of men with persistent oligospermia after retrograde embolization for varicocele?. World Journal of Urology 2008; Vol. 26, issue 1:97-102.
32. Gamidov SI, Ovchinnikov RI, Popova AY, Avakyan AY, Sukhikh GT. Adjuvant antioxidant therapy in varicocele infertility. [Russian]. Urologiia 2017; Vol. 2:64-72.
33. Gamidov SI, Ovchinnikov RI, Popova AY, Avakyan AY, Sukhikh GT. [Adjuvant antioxidant therapy in varicocele infertility]. Urologiia (Moscow, Russia) 2017, issue 2 (supplement):64-72.
34. Gopinath PM, Kalra B, Saxena A, Malik S, Kochhar K, Kalra S, et al. Fixed dose combination therapy of antioxidants in treatment of idiopathic oligoasthenozoospermia: Results of a randomized, double-blind, placebo-controlled clinical trial. International Journal of Infertility and Fetal Medicine 2013; Vol. 4, issue 1:6-13.
35. Greco E, Iacobelli M, Rienzi L, Ubaldi F, Ferrero S, Tesarik J. Reduction of the incidence of sperm DNA fragmentation by oral antioxidant treatment. Journal of Andrology 2005; Vol. 26, issue 3:349-53.
36. Haghighian HK, Haidari F, Mohammadi-Asl J, Dadfar M. Randomized, triple-blind, placebo-controlled clinical trial examining the effects of alpha-lipoic acid supplement on the spermatogram and seminal oxidative stress in infertile men. Fertility & Sterility 2015; Vol. 104, issue 2:318-24.
37. Haje M, Naoom K. Combined tamoxifen and L-carnitine therapies for the treatment of idiopathic male infertility attending intracytoplasmic sperm injection: A randomized controlled trial. International Journal of Infertility and Fetal Medicine 2015; Vol. 6, issue 1:20-4.
38. Kessopoulou E, Powers HJ, Sharma KK, Pearson MJ, Russell JM, Cooke ID, et al. A double-blind randomized placebo cross-over controlled trial using the antioxidant vitamin E to treat reactive oxygen species associated male infertility. Fertility and Sterility 1995; Vol. 64, issue 4: 825-31.
39. Kumamoto Y, Maruta H, Ishigami J, Kamidono S, Orikasa S, Kimura M, et al. Clinical efficacy of mecobalamin in the treatment of oligozoospermia--results of double-blind comparative clinical study. Hinyokika Kiyo. Acta Urologica Japonica 1988;34:1109-32.
40. Lenzi A, Lombardo F, Sgro P, Salacone P, Caponecchia L, Dondero F, et al. Use of carnitine therapy in selected cases of male factor infertility: a double-blind crossover trial [see comment]. Fertility and Sterility 2003; Vol. 79, issue 2: 292-300.
41. Lenzi A, Sgro P, Salacone P, Paoli D, Gilio B, Lombardo F, et al. A placebo-controlled double-blind randomized trial of the use of combined l-carnitine and l-acetyl-carnitine treatment in men with asthenozoospermia [see comment]. Fertility and Sterility 2004; Vol. 81, issue 6:1578-84.
42. Li Z, Chen GW, Shang XJ, Bai WJ, Han YF, Chen B, et al. A controlled randomized trial of the use of combined L-carnitine and acetyl-L-carnitine treatment in men with oligoasthenozoospermia. Zhong Hua Nan Ke Xue 2005; Vol. 11, issue 10:761-4.
43. Li Z, Gu R, Liu Y, Xiang Z, Cao X, Han Y, et al. Curative effect of L-carnitine supplementation in the treatment of male infertility. Academic Journal of Shanghai Second Medical University 2005; Vol. 25, issue 3:292-4.
44. Lombardo F, Gandini L, Agarwal A, Sgro P, Dondero F, Lenzi A. A prospective double blind placebo controlled cross over trial of carnitine therapy in selected cases of male infertility. Fertility and Sterility 2002; Vol. 78 Suppl 1: 68-9.
45. Martinez AM, Sordia-Hernandez LH, Morales JA, Merino M, Vidal O, Garcia Garza MR, et al. A randomized clinical study assessing the effects of the antioxidants, resveratrol or SC1002, a hydrogen sulfide prodrug, on idiopathic oligoasthenozoospermia. Asian Pacific Journal of Reproduction 2015; Vol. 4, issue 2:106-11.
46. Martinez-Soto JC, Domingo JC, Cardobilla LP, Pellicer A, Landeras J. Effect of dietary DHA supplementation on sperm DNA integrity. Fertility and Sterility September 2010;94(4):S235-6.
47. Mehni NM, Ketabchi AA, Hosseini E. Combination effect of pentoxifylline and L-carnitine on idiopathic oligoasthenoteratozoospermia. Iranian Journal of Reproductive Medicine 2014; Vol. 12, issue 12:817-24.
48. Micic S, Lalic N, Bojanic N, Djordjevic D, Virmani A, Agarwal A. Assessment of sperm motility in oligoasthenospermic men, treated with metabolic and essential nutrients, in a randomized, double blind, placebo study. Human Reproduction 2016; Vol. 31 (Supplement 1):i151-2.
49. Micic S, Lalic N, Bojanic N, Djordjevic D, Virmani A, Agarwal A. DBPC study in oligoasthenospermic men treated with metabolic and essential nutrients showed that progressive sperm motility was correlated to seminal carnitine levels. Andrology 2016; Vol. 4 (Supplement 2): 56.
50. Micic S, Lalic N, Bojanic N, Djordjevic D, Virmani A, Agarwal A. DBPC study showed significant correlation of DNA fragmetation index (DFI) and seminal carnitine with progressive sperm motility in oligospermic men treated with metabolic and essential nutrients. Fertility and Sterility 2017; Vol. 108 (3 Supplement 1):e307-8.
51. Micic S, Lalic N, Bojanic N, Djordjevic D, Virmani A, Agarwal A. Oligoastenospermic men treated with proxeed plus showed correlation between sperm motility and seminal carnitine. Fertility and Sterility 2016; Vol. 106 (Supplement 3):e298-9.
52. Micic S, Lalic N, Djordjevic D, Bogavac-Stanojevic N, Virmani A, Agarwal A. Seminal carnitine and DNA fragmentation index (DFI) impact progressive sperm motility in oligoasthenospermic men treated with metabolic and essential nutrients, with moderate accuracy. Human Reproduction 2017; Vol. 32 (Supplement 1):i132.
53. Morgante G, Scolaro V, Tosti C, Di Sabatino A, Piomboni P, De Leo V. Treatment with carnitine, acetyl carnitine, L-arginine and ginseng improves sperm motility and sexual health in men with asthenopermia. Minerva Urologica e Nefrologica 2010;62(3):213-8.
54. Nadjarzadeh A, Shidfar F, Amirjannati N, Vafa MR, Motevalian SA, Gohari MR, et al. Coenzyme Q10 improves seminal oxidative defense but does not affect on seman in idiopathic oligoasthenoteratozoospermia: A randomized double- blind placebo controlled trial. Journal of Endocrinological Investigation 2011;34:e224-8.
55. Nozha CF, Leila AK, Zouhir S, Hanen G, Khled Z, Tarek R. Oxidative stress and male infertility: comparative study of combined vitamin E/selenium treatment versus vitamin B. Human Reproduction 2001; Vol. 17, issue Abstract book 1:111-2.
56. Omu AE, Dashti H, Al-Othman S. Treatment of asthenozoospermia with zinc sulphate: andrological, immunological and obstetric outcome. European Journal of Obstetrics, Gynecology, and Reproductive Biology 1998; Vol. 79, issue 2:179-84.
57. Omu AE, Al-Azemi MK, Kehinde EO, Anim JT, Oriowo MA, Mathew TC. Indications of the mechanisms involved in improved sperm parameters by zinc therapy. Medical Principles and Practice 2008; Vol. 17, issue 2:108-16.
58. Peivandi S, Abasali K, Narges M. Effects of L-carnitine on infertile men's spermogram; a randomised clinical trial. Journal of Reproduction and Infertility 2010;10(4):331.
59. Pourmand G, Movahedin M, Dehghani S, Mehrsai A, Ahmadi A, Pourhosein M, et al. Does L-carnitine therapy add any extra benefit to standard inguinal varicocelectomy in terms of deoxyribonucleic acid damage or sperm quality factor indices: a randomized study. Urology 2014; Vol. 84, issue 4:821-5.
60. Pourmand GH, Movahedin M, Dehghan S, Mehrsai A, Ahmadi A, Pourhosein M. Does L-carnitine therapy add any extra benefit to standard inguinal varicocelectomy in terms of deoxyribonucleic acid damage or sperm quality factor indices: a randomized study. Iranian Journal of Reproductive Medicine. 2015, issue 4 suppl. 1:67.
61. Poveda C, Rodriguez R, Chu EE, Aparicio LE, Gonzales IG, Moreno CJ. A placebo-controlled double-blind randomized trial of the effect of oral supplementation with spermotrend, maca extract (lepidium meyenii) or L-carnitine in semen parameters of infertile men. Fertility and Sterility 2013; Vol. 100, issue 3 Suppl:S440.
62. Pryor JP, Blandy JP, Evans P, Chaput de Saintonge DM, Usherwood M. Controlled clinical trial of arginine for infertile men with oligozoospermia. British Journal of Urology 1978;50:47-50.
63. Raigani M, Yaghmaei B, Amirjannti N, Lakpour N, Akhondi MM, Zeraati H, et al. The micronutrient supplements, zinc sulphate and folic acid, did not ameliorate sperm functional parameters in oligoasthenoteratozoospermic men. Andrologia 2014; Vol. 46, issue 9:956-62.
64. Rolf C, Cooper TG, Yeung CH, Nieschlag E. Antioxidant treatment of patients with asthenozoospermia or moderate oligoasthenozoospermia with high-dose vitamin C and vitamin E: a randomized, placebo-controlled, double-blind study. Human Reproduction 1999;14(4):1028-33.
65. Rolf C, Cooper TG, Yeung CH, Nieschlag E. Antioxidant treatment of patients with asthenozoospermia or moderate oligoasthenozoospermia with high-dose vitamin C and vitamin E: a randomized, placebo-controlled, double-blind study [see comment]. Human Reproduction 1999;14(4): 1028-33.
66. Safarinejad MR, Safarinejad S. Efficacy of selenium and/ or N-acetyl-cysteine for improving semen parameters in infertile men: a double-blind, placebo controlled, randomized study. Journal of Urology 2009;181(2):741-51.
67. Safarinejad ME. Efficacy of coenzyme Q10 on semen parameters, sperm function and reproductive hormones in infertile men. Journal of Urology 2009;182(1):237-48.
68. Safarinejad MR, Shafiei N, Safarinejad S. Effects of the reduced form of coenzyme Q10 (ubiquinol) on semen parameters in men with idiopathic infertility: A double-blind, placebo controlled, randomized study. Journal of Urology 2012;188(2):526-31.
69. Scott R, MacPherson A, Yates RW, Hussain B, Dixon J. The effect of oral selenium supplementation on human sperm motility. British Journal of Urology 1998; Vol. 82, issue 1: 76-80.
70. Sharifzadeh F, Norouzi S, Ashrafi M, Aminimoghaddam S, Koohpayezadeh J. Effects of zinc sulfate on subfertility related to male factors: a prospective double-blind, randomized, placebo-controlled clinical trial. Journal of Obstetrics, Gynecology and Cancer Research 2016; Vol. 1, issue 2:e7242. 2476-5848]
71. Pryor JL, Stacy L, Glass S, Campagnone J, Sigman M. Randomized double blind placebo controlled trial of carnitine for the treatment of idiopathic asthenospermia. Fertility and Sterility 2003;80 Suppl 3:48.
72. Sigman M, Glass S, Campagnone J, Pryor JL. Carnitine for the treatment of idiopathic asthenospermia: a randomized, double-blind, placebo-controlled trial. Fertility and Sterility 2006;85(5):1409-14.
73. Sivkov AV, Oshchepkov VN, Evdokimov VV, Keshishev NG, Skabko OV. Selzink plus study in patients with chronic non-infectious prostatitis and abnormal fertility. [Russian]. Urologii 2011; Vol. 5:27-33.
74. Sofikitis N, Dimitriadis F, Skouros S, Stavrou S, Seminis G, Giannakis I, et al. Effects of avanafil on semen quality and sperm cytoskeleton in oligoasthenospermic infertile men: A randomized controlled trial. Journal of Sexual Medicine 2016; Vol. 2:S164-5.
75. Suleiman SA, Ali ME, Zaki ZM, el-Malik EM, Nasr MA. Lipid peroxidation and human sperm motility: protective role of vitamin E. Journal of Andrology 1996; Vol. 17, issue 5:530-7.
76. Tremellen K, Froiland D, Miari G, Thompson J. A randomised control trial examining the effect of an antioxidant on sperm function pregnancy outcome during IVF treatment. The Fertility Society of Australia 25th Annual scientific meeting. Australian & New Zealand Journal of Obstetrics & Gynaecology 2006;46 Suppl 2:A.2.
77. Tremellen K, Miari G, Froiland D, Thompson J. A randomised control trial examining the effect of an antioxidant (Menevit) on pregnancy outcome during IVF-ICSI treatment. Australian & New Zealand Journal of Obstetrics & Gynaecology 2007;47:216-21.
78. Wang YX, Yang SW, Qu CB, Huo HX, Li W, Li JD, et al. L-carnitine: safe and effective for asthenozoospermia. Zhonghua nan ke xue - National Journal of Andrology 2010; 16(5):420-2.
79. Ebisch IM, Pierik FH, De Jong FH, Thomas CM, Steegers-Theunissen RP. Does folic acid and zinc sulphate intervention affect endocrine parameters and sperm characteristics in men?. International Journal of Andrology 2006;29(2):339-45.
80. Wong WY, Merkus HM, Thomas CM, Menkveld R, Zielhuis GA, Steegers-Theunissen RP. Effects of folic acid and zinc sulfate on male factor subfertility: a double-blind, randomized, placebo-controlled trial. Fertility and Sterility 2002; Vol. 77, issue 3:491-8.
81. Zalata A, Christophe A, Horrbin D, Dhooge W, Comhaire F. Protection of sperm function and DNA by essential fatty acids and antioxidants dietary supplementation. Fertility and Sterility 1998;70:287.
82. Zalata A, Christophe A, Horrobin D, Dhooge W, Comhaire F. Effect of essential fatty acids and antioxidants dietary supplementation on the oxidative DNA damage of the human spermatozoa. ESHRE 14th annual meeting. Goteborg, 1998:270-1.
83. Zavaczki Z, Szollosi J, Kiss S, Koloszar S, Fejes I, Kovacs L, Pal A. Magnesium-orotate supplementation for idiopathic infertile male patients: a randomized, placebo-controlled clinical pilot study. Magnesium Research 2003;16(2):131-6.
84. Adel N, El Maghraby H, Ghareeb D, Elmahdy M. Vitamin e and berberine counteract the adverse effects of ros on sperm and seminal parameters. Human Reproduction 2015; 30 Suppl 1:i171 Abstract no: P-113.
85. Alahmar AT. Effect of vitamin C, vitamin E, zinc, selenium, and coenzyme Q10 in infertile men with idiopathic oligoasthenozoospermia. International Journal of Infertility and Fetal Medicine 2017;8(2):45-9.
86. Alizadeh F, Javadi M, Karami AA, Gholaminejad F, Kavianpour M, Haghighian HK. Curcumin nanomicelle improves semen parameters, oxidative stress, inflammatory biomarkers, and reproductive hormones in infertile men: A randomized clinical trial. Phytotherapy Research 2018;32(3): 514-21.
87. Alsalman AR, Almashhedy LA, Hadwan MH. Effect of oral zinc supplementation on the thiol oxido-reductive index and thiol-related enzymes in seminal plasma and spermatozoa of Iraqi asthenospermic patients. Biological Trace Element Research 2018;184(2):340-9.
88. Anarte C, Calvo I, Domingo A, Presilla N, Aleman M, Bou R, et al. Effect of DHA supplementation on fatty acid composition of sperm and its relation to semen quality. Human Reproduction - Abstract book of the 28th ESHRE Annual Meeting, Turkey 1-4 July 2012. 2012; Vol. 27, issue 2:ii121-ii50.
89. Anarte C, Domingo A, Agirregoikoa JA, De Pablo J, Barrenetxea G. Regular consumption of docosahexaenoic acid (DHA) improves semen quality. Fertility and Sterility 2013;100 Suppl 3:S443.
90. Cai T, Wagenlehner F, Mazzoli S, Meacci F, Mondaini N, Nesi G, et al. Semen quality in patients with Chlamydia trachomatis genital infection treated concurrently with prulifloxacin and a phytotherapeutic agent. Journal of Andrology 2012;33(4):615-23.
91. Calogero AE, Gullo G, La Vignera S, Condorelli RA, Vaiarelli A. Myoinositol improves sperm parameters and serum reproductive hormones in patients with idiopathic infertility: a prospective double-blind randomized placebo-controlled study. Andrology 2015;3(3):491-5.
92. Capece M, Romeo G, Ruffo A, Romis L, Mordente S, Di Lauro G. A phytotherapic approach to reduce sperm DNA fragmentation in patients with male infertility. Urologia (Treviso) 2017;84(2):79-82.
93. Capece M, Romeo G, Ruffo A, Romis L, Mordente S, Di Lauro G. Alga Ecklonia Bicyclis, tribulus terrestris, myoinositol and biovistm reduce sperm DNA fragmentation in patients with oligo-asthenoterato-zoospermia (OAT) syndrome. Journal of Sexual Medicine 2015;12 (Supplement 5):365.
94. Chattopadhyay R, Yasmin S, Chakravarty BN. Effect of continuous 6 months oral antioxidant combination with universally recommended dosage in idiopathic male infertility. International Journal of Infertility and Fetal Medicine 2016;7(1):1-6.
95. Chen XF, Li Z, Ping P, Dai JC, Zhang FB, Shang XJ, et al. Efficacy of natural vitamin E on oligospermia and asthenospermia: a prospective multi-centered randomized controlled study of 106 cases. Zhong Hua Nan Ke Xue 2012; 18(5):428-31.
96. Ciftci H, Verit A, Savas M, Yeni E, Erel O. Effects of N-acetylcysteine on semen parameters and oxidative/ antioxidant status. Urology 2009;74(1):73-6.
97. Comhaire FH, El Garem Y, Mahmoud A, Eertmans F, Schoonjans F. Combined conventional/antioxidant “Astaxanthin” treatment for male infertility: a double blind, randomized trial. Asian Journal of Andrology 2005;7(3): 257-62.
98. Ebisch IM, van Heerde WL, Thomas CM, van der Put N, Wong WY, Steegers-Theunissen RP. C677T methylenetetrahydrofolate reductase polymorphism interferes with the effects of folic acid and zinc sulfate on sperm concentration. Fertility and Sterility 2003;80(5): 1190-4.
99. Elgindy EA, El-Huseiny AM, Mostafa MI, Gaballah AM, Amed TA. N-Acetylcysteine: Could it be an effective adjuvant therapy in ICSI cycles. Fertility and Sterility 2008;90 Suppl 1(American Society for Reproductive Medicine 64th Annual Meeting. 8-12 November 2008, San Francisco, CA):356 Abstract no: P738.
100. Ghafarizadeh AA, Vaezi G, Shariatzadeh MA, Malekirad AA. Effect of in vitro selenium supplementation on sperm quality in asthenoteratozoospermic men. Andrologia 2018; 50(2):e12869.
101. Ghanem H, Shaeer O, El-Segini A. Combination clomiphene citrate and antioxidant therapy for idiopathic male infertility: a randomized controlled trial. Fertility and Sterility 2010;93(7):2232-5.
102. Gulati S, Chattopadhyay R, Ghosh B, Yasmin S, Ghosh S, Bose G, et al. Treatment with combined antioxidant formulation before ICSI improves pregnancy rate in couples with obstructive azoospermia. Fertility and Sterility 2015; 1):e241.
103. AGUNCO Obstetrics and Gynecology Centre (PI unknown). Effect of treatment with myo-inositol on human semen parameters in patients undergoing in vitro fertilization cycles. https://clinicaltrials.gov.
104. Gulino FA, Leonardi E, Marilli I, Musmeci G, Vitale SG, Leanza V, et al. Effect of treatment with myo-inositol on semen parameters of patients undergoing an IVF cycle: in vivo study. Gynecological Endocrinology 2016;32(1):65-8.
105. Hafeez M, Ahmed A, Usmanghani K, Mohiuddin E, Asif HM, Akram M, et al. Clinical evaluation of herbal medicine for oligospermia. Pakistan Journal of Nutrition 2011;10(3): 238-40.
106. Iacono F, Ruffo A, Prezioso D, Illiano E, Romeo G, Romis L, et al. Combination therapy with antiestrogen and a natural composite containing tribulus terrestris, alga ecklonia bicyclis, biovis and myo-inositol in the treatment of male idiopathic infertility. Journal of Sexual Medicine 2014;1:92.
107. Jawad HM. Zinc sulfate treatment of secondary male infertility associated with positive serum and seminal plasma anti-sperm antibody test. Middle East Fertility Society Journal 2013;18(1):24-30.
108. Kanta Goswami S, Chattopadhyay R, Ghosh S, Ghosh B, Yasmin S, Goswami M, et al. Exogenous oral combination antioxidants in idiopathic infertile males, having higher DNA fragmentation index, before ICSI cycle may improve live birth rate. Human Reproduction 2017;32 (Supplement 1):i160.
109. Keskes-Ammar L, Feki-Chakroun N, Rebai T, Sahnoun Z, Ghozzi H, Hammami S, et al. Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Archives of Andrology 2003; 49(2):83-94.
110. Kim C-H, Yoon J-W, Ahn J-W, Kang H-J, Lee J-W, Kang B-M. The effect of supplementation with omega-3-polyunsaturated fatty acids in intracytoplasmic sperm injection cycles for infertile patients with a history of unexplained total fertilization failure. Fertility and Sterility 2010;94(4 Suppl 1):S242.
111. Korosi T, Barta C, Rokob K, Torok T. Physiological Intra-Cytoplasmic Sperm Injection (PICSI) outcomes after oral pretreatment and semen incubation with myo-inositol in oligoasthenoteratozoospermic men: results from a prospective, randomized controlled trial. European Review for Medical & Pharmacological Sciences 2017;21(2 Suppl): 66-72.
112. Kumar R, Saxena V, Shamsi M, Venkatesh S, Dada R. Herbo-mineral supplementation in men with idiopathic oligoasthenoteratospermia: A double blind randomized placebo-controlled trial. Indian Journal of Urolology 2011; 27(3):357-62.
113. Lenzi A, Culasso F, Gandini L, Lombardo F, Dondero F. Placebo-controlled, double-blind, cross-over trial of glutathione therapy in male infertility. Human Reproduction 1993;8(10):1657-62.
114. Lu SM, Li X, Zhang HB, Hu JM, Yan JH, Liu JL, et al. [Use of L-carnitine before percutaneous epididymal sperm aspiration-intracytoplasmic sperm injection for obstructive azoospermia]. Zhonghua nan ke xue - National Journal of Andrology 2010;16(10):919-21.
115. Martinez-Soto JC, Domingo JC, Cordobilla B, Nicolas M, Fernandez L, Albero P, et al. Dietary supplementation with docosahexaenoic acid (DHA) improves seminal antioxidant status and decreases sperm DNA fragmentation. Systems Biology in Reproductive Medicine 2016;62(6):387-95.
116. Merino G, Martinez Chequer JC, Barahona E, Bermudez JA, Moran C, Carranza-Lira S. Effects of pentoxifylline on sperm motility in normogonadotropic asthenozoospermic men. Archives of Andrology 1997;39(1):65-9.
117. Micic S, Hadzi-Djokic J, Dotlic R, Tulic C. Pentoxifyllin treatment of oligoasthenospermic men. Acta Europaea Fertilitatis 1988;19(3):135-7.
118. Micic S, Lalic N, Bojanic N, Nale DJ. Oligospermic men treated by carnitine. 17th World Congress on Fertility and Sterility. Melbourne, 2001:38.
119. Movahedin M, Mehrsai A, Noori M, Dehghani S, Pourmand G, Ahmadi A, et al. Does anti-oxidant therapy add any extra benefit to standard inguinal varicocelectomy in terms of DNA damage or sperm quality factor indices: a randomized study. Urology 2014;4(suppl. 1):S252.
120. Nadjarzadeh A, Shidfar F, Amirjannati N, Vafa MR, Motevalian SA, Gohari MR, et al. Effect of Coenzyme Q10 supplementation on antioxidant enzymes activity and oxidative stress of seminal plasma: a double-blind randomised clinical trial. Andrologia 2014;46(2):177-83.
121. Nashivochnikova NA, Krupin VN, Selivanova SA. [Efficiency of spematon in male infertility]. Urologiia (Moscow, Russia: 1999) 2014;2:52-4. PUBMED: 24956674]
122. Tsafrir A. Is a carnitine based food supplement (PorimoreTM) for infertile men superior to folate and zinc with regard to pregnancy rates in intrauterine insemination cycles?. https://clinicaltrials.gov.
123. Irge D. Supplement intake in infertile men; the effect on sperm parameters, fertilization rate and embryo quality. https://clinicaltrials.gov.
124. Nematollahi-Mahani SN, Azizollahi GH, Baneshi MR, Safari Z, Azizollahi S. Effect of folic acid and zinc sulphate on endocrine parameters and seminal antioxidant level after varicocelectomy. Andrologia 2014;46(3):240-5.
125. Niederberger C. Combination clomiphene citrate and antioxidant therapy for idiopathic male infertility: a randomized controlled trial. Journal of Urology 2011;185 (1):252.
126. Nikolova V, Stanislavov R, Vatev I, Nalbanski B, Punevska M. Sperm parameters in male idiopathic infertility after treatment with prelox. Akusherstvo i Ginekologiia 2007;46 (5):7-12.
127. Pawlowicz P, Stachowiak G, Bielak A, Wilczynski J. Administration of natural anthocyanins derived from chokeberry (aronia melanocarpa) extract in the treatment of oligospermia in males with enhanced autoantibodies to oxidized low density lipoproteins (oLAB). The impact on fructose levels. Ginekologia Polska 2001;72(12):983-8.
128. Polak de Fried E, Bossi NM, Notrica JA, Vazquez Levin MH. Vitamin-d treatment does not improve pregnancy rates in patients undergoing art: a prospective, randomized, double-blind placebo-controlled trial. Fertility and Sterility 2013;100 Suppl 3:S493-4.
129. Raigani M, Sadeghi MR, Akhondi MA, Amir Jannati N, Soleimani Badia M. Impacts of MTHFR polymorphism on the effects of folic acid and zinc sulfate supplementations in OAT men. Iranian Journal of Reproductive Medicine 2010; 1):46.
130. Safarinejad MR. Effect of pentoxifylline on semen parameters, reproductive hormones and seminal plasma antioxidant capacity in men with idiopathic infertility: a randomized double-blind placebo-controlled study. International Urology and Nephrology 2011;43:315-28.
131. Safarinejad MR, Shafiei N, Safarinejad S. A prospective double-blind randomized placebo-controlled study of the effect of saffron (Crocus sativus Linn.) on semen parameters and seminal plasma antioxidant capacity in infertile men with idiopathic oligoasthenoteratozoospermia. Phytotherapy research: PTR 2011;25(4):508-16.
132. Singh AK, Deshpande SB. Role of nutraceuticals in treatment of azoospermia due to maturation arrest. Indian Journal of Physiology and Pharmacology 2016;60 (5 Supplement 1):6-7.
133. Soylemez H, Kilic S, Atar M, Penbegul N, Sancaktutar AA, Bozkurt Y. Effects of micronised purified flavonoid fraction on pain, semen analysis and scrotal color Doppler parameters in patients with painful varicocele; results of a randomized placebo-controlled study. International Urology and Nephrology 2012;44(2):401-8.
134. Stanislavov R, Nikolova V, Rohdewald P. Improvement of seminal parameters with Prelox®: a randomized, double-blind, placebo controlled, cross over trial. Phytotherapy Research 2009;23:297-302. DOI: 10.1002
135. Stanislavov R, Rohdewald P. Sperm quality in men is improved by supplementation with a combination of L-arginine, L-citrullin, roburins and Pycnogenol. Minerva Urologica e Nefrologica 2014;66(4):217-23.
136. Stanislavov R, Rohdewald P. Sperm quality in men is improved by supplementation with a combination of L-arginine, L-citrulline, roburins and Pycnogenol. Minerva Urologica e Nefrologica 2014;66(4):217-23.
137. Stanislavov R, Rohdewald P. Sperm quality in men is improved by supplementation with a combination of L-arginine, L-citrulline, roburins and Pycnogenol. Minerva Urologica e Nefrologica 2014;66(4):217-23.
138. Tang KF, Xing Y, Wu CY, Liu RZ, Wang XY, Xing JP. [Tamoxifen combined with coenzyme Q10 for idiopathic oligoasthenospermia]. Zhong Hua Nan Ke Xue 2011;17(7): 615-8.
139. Verzeletti FB, Poletto RS, Bertolin TE, Fornari F. Evaluation of sperm quality in adults after use spirulina platensis and resveratrol. Jornal Brasileiro de Reproducao Assistida 2012;16 (5):271-7.
140. Vicari E, Calogero AE. Effects of treatment with carnitines in infertile patients with prostatovesiculoepididymitis and elevated sperm oxidative stress. ESHRE 17th Annual Meeting. Lausanne: Human Reproduction, 2001; Vol. 16 Suppl 1:106-7.
141. Vicari E, Rubino C, De Palma A, Longo G, Lauretta M, Consoli S, et al. Antioxidant therapeutic efficiency after the use of carnitine in infertile patients with bacterial or non bacterial prostato-vesiculo-epididymitis. Archivio Italiano di Urologia, Andrologia 2001;73(1):15-25.
142. Vicari E, La Vignera S, Calogero AE. Antioxidant treatment with carnitines is effective in infertile patients with prostatovesiculoepididymitis and elevated seminal leukocyte concentrations after treatment with nonsteroidal anti-inflammatory compounds. Fertility and Sterility 2002;78 (6):1203-8.
143. Wang C, Chan CW, Wong KK, Yeung KK. Comparison of the effectiveness of placebo, clomiphene citrate, mesterolone, pentoxifylline, and testosterone rebound therapy for the treatment of idiopathic oligospermia. Fertility and Sterility 1983;40(3):358-65.
144. Wang Y, Yang S, Cai W, Qu C, Li J, Chang X, et al. [Clinical efficacy of L-carnitine combined with tamoxifen in treatment of oligoasthenozoospermia] LA: Chi. Zhonghua nan ke xue - National Journal of Andrology 2010;16(5): 420-2.
145. Wu ZM, Lu X, Wang YW, Sun J, Tao JW, Yin FH, et al. Short-term medication of L-carnitine before intracytoplasmic sperm injection for infertile men with oligoasthenozoospermia. Zhong Hua Nan Ke Xue 2012;18 (3):253-6.
146. Goswami SK, Yasmin S, Chakraborty P, Chattopadhyay R, Ghosh S, Goswami M, et al. Role of dietary antioxidant supplementation in treatment of idiopathic male infertility: Promising evidence from a sub-continental study. Fertility and Sterility 2015;1):e288.
147. Kamath MS. To compare the effectiveness of antioxidants versus no treatment for male partner for improving pregnancy rates in couples undergoing In vitro fertilization (IVF) for abnormal semen analysis. www.who.int/trialsearch.
148. Baumgraß H. Randomized, placebo-controlled, double-blind, multi-centre pilot study to investigate the effect of AM019016 on male spermatogenesis in subjects with diagnosed unspecific (idiopathic) subfertility. www.who.int/trialsearch.
149. Talebi A, Amini L. The effect of oral vitamin D3 supplementation on spermogram quantitative and qualitative indicators in infertile male. www.who.int/trialsearch.
150. Azima S. The effects of folic acid, vitamin E, selenium on semen parameters in infertile men [Effect of folic acid, vitamin E, selenium on semen parameters]. www.who.int/trialsearch.
151. Aguilar MM. Assessment of the efficacy of dietary supplement spermotrend in the treatment of male infertility. ClinicalTrials.gov.
152. Mathieu-d'Argent E. Impact of folates in the care of the male infertility (FOLFIV). https://clinicaltrials.gov.
153. Palumbo M. Myo-inositol on human semen parameters [Effect of of treatment with Myo-inositol on human semen parameters in patients undergoing IVF cycles]. https://clinicaltrials.gov.
154. Hekmatdoost A. The effects of administration of combined docosahexaenoic acid and vitamin e supplements on spermatogram and seminal plasma oxidative stress in infertile men with asthenozoospermia. https://clinicaltrials.gov.
155. Pinter B, Imamovic Kumalic S. Oral Astaxanthin and semen quality, fertilization and embryo development in Assisted Reproduction Techniques procedures. https://clinicaltrials.gov.
156. Eisenberg E, Steiner AZ. Males, antioxidants, and infertility trial (MOXI). https://clinicaltrials.gov.
157. Jawaid M. Neotililty trial: Effect of coenzyme Q10 on semen parameters in men with idiopathic infertility (neotility). https://clinicaltrials.gov.
158. Smits RM, Fleischer K, Braat DDM. The impact of a nutritional supplement (Impryl®) on male fertility. https://clinicaltrials.gov.
159. Agarwal A, Said T. Role of sperm chromatin abnormalities and DNA damage in male infertility. Human Reproduction Update 2003;9(4):331-45.
160. Agarwal A, Nallella KP, Allamaneni S, Said TM. Role of antioxidants in treatment of male infertility: an overview of the literature. Reproductive Biomedicine Online 2004;8(6): 616-27.
161. Agarwal A, Said TM. Carnitines and male infertility. Reproductive BioMedicine Online (Reproductive Healthcare Limited). Reproductive Healthcare Limited, 2004; Vol. 8, issue 4:376-84.
162. Agarwal A, Majzoub A, Esteves SC, Ko EY, Ramasamy R, Zini A. Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios. Translational Andrology and Urology 2016;5(6): 935-50.
163. Agarwal A, Majzoub A. Laboratory tests for oxidative stress. Indian Journal of Urology 2017;33(3):199-206. [0970-1591: (Print)]
164. Aitken RJ, Clarkson JS. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. Journal of Reproduction and Fertility 1987;81(2):459-69. [0022-4251: (Print)]
165. Aitken RJ, Clarkson JS, Hargreave TB, Irvine DS, Wu FC. Analysis of the relationship between defective sperm function and the generation of reactive oxygen species in cases of oligozoospermia. Journal of Andrology 1989;10(3): 214-20. [0196-3635: (Print)]
166. Aitken RJ, West KM. Analysis of the relationship between reactive oxygen species production and leucocyte infiltration in fractions of human semen separated on Percoll gradients. International Journal of Andrology. Blackwell Publishing Ltd, 1990; Vol. 13, issue 6:433-51.
167. Aitken RJ, Buckingham D, West K, Wu FC, Zikopoulos K, Richardson DW. Differential contribution of leucocytes and spermatozoa to the generation of reactive oxygen species in the ejaculates of oligozoospermic patients and fertile donors. Journal of Reproduction and Fertility 1992;94(2):451-62. [0022-4251: (Print)]
168. Aitken J, Fisher H. Reactive oxygen species generation and human spermatozoa: the balance of benefit and risk. Bioessays 1994;16(4):259-67. [0265-9247: (Print)]
169. Aitken RJ, De Iuliis GN, Finnie JM, Hedges A, McLachlan RI. Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: development of diagnostic criteria. Human Reproduction 2010;25(10):2415-26.
170. Aktan G, Dogru-Abbasoglu S, Kucukgergin C, Kadioglu A, Ozdemirler-Erata G, Kocak-Toker N. Mystery of idiopathic male infertility: is oxidative stress an actual risk?. Fertility and Sterility 2013;99(5):1211-5.
171. Alvarez 2003. Nuture vs nature: How can we optimise sperm quality?. Journal of Andrology 2003;24(5):640-8.
172. Annals of the New York Academy of Science. Preface: Carnitine: Lessons from One Hundred Years of Research. Annals of the New York Academy of Science. Blackwell Publishing Ltd, 2004; Vol. 1033, issue 1:ix-xi.
173. Appleton J. Arginine: clinical potential of a semi-essential amino acid. Alternative Medicine Review 2002;7(6):512-22. [10895159]
174. Atkuri KR, Mantovani JJ, Herzenberg LA, Herzenberg LA. N-Acetylcysteine-a safe antidote for cysteine/glutathione deficiency. Cancer/Immunomodulation 2007;7(4):355-9.
175. Barratt CLR, Bjorndahl L, De Jonge CJ, Lamb DJ, Osorio Martini F, McLachlan R, et al. The diagnosis of male infertility: an analysis of the evidence to support the development of global WHO guidance-challenges and future research opportunities. Human Reproduction Update 2017;23(6):660-80. [1460-2369: (Electronic)]
176. Bendich A, Olson JA. Biological actions of carotenoids. The FASEB Journal 1989;3(8):1927-32.
177. Bertelli AA, Das DK. Grapes, wines, resveratrol, and heart health. Journal of Cardiovascular Pharmacology 2009;54(6): 468-76.
178. Bevilacqua A, Carlomagno G, Gerli S, Montanino Oliva M, Devroey P, Lanzone A, et al. Results from the International Consensus Conference on myo-inositol and D-chiro-inositol in Obstetrics and Gynecology - assisted reproduction technology. Gynecological Endocrinology 2015; 31(6):441-6.
179. Bhagavan HN, Chopra RK. Coenzyme Q10: Absorption, tissue uptake, metabolism and pharmacokinetics. Free Radical Research 2006;40(5):445-53.
180. Boe-Hansen GB, Fedder J, Ersboll AK, Christensen P. The sperm chromatin structure assay as a diagnostic tool in the human fertility clinic. Human Reproduction 2006;21(6): 1576-82.
181. Boitani C, Puglisi R. Selenium, a key element in spermatogenesis and male fertility. Molecular Mechanisms in Spermatogenesis. New York, NY: Springer New York, 2008: 65-73.
182. Boivin J, Bunting L, Collins JA, Nygren KG. International estimates of infertility prevalence and treatment-seeking: potential need and demand for infertility medical care. Human Reproduction 2007;22(6):1506-12. [0268-1161]
183. Borini A, Tarozzi N, Nadalini M. Sperm DNA fragmentation testing in male infertility work-up: are we ready?. Translational Andrology and Urology 2017;6(Suppl 4):S580-2.
184. Branco CS, Garcez ME, Pasqualotto FF, Erdtman B, Salvador M. Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen. Cryobiology 2010;60(2):235-7.
185. Bungum M, Humaidan P, Spano M, Jepson K, Bungum L, Giwercman A. The predictive value of sperm chromatin structure assay (SCSA) parameters for the outcome of intrauterine insemination, IVF and ICSI. Human Reproduction 2004;19(6):1401-8.
186. Burk RF. Selenium, an antioxidant nutrient. Nutrition in Clinical Care 2002;5(2):75-9.
187. Bykova M, Athayde K, Sharma R, Jha R, Sabanegh E, Agarwal A. Defining the reference value of seminal reactive oxygen species in a population of infertile men and normal healthy volunteers. Fertility and Sterility 2007;88 Suppl 1 (P-597):305.
188. Bøhmer T, Hoel P, Purvis K, Hansson V. Carnitine levels in human accessory sex organs. Archives of Andrology 1978;1 (1):53-9.
189. Cissen M, Wely M, Scholten I, Mansell S, Bruin JP, Mol BW, et al. Measuring sperm DNA fragmentation and clinical outcomes of medically assisted reproduction: a systematic review and meta-analysis. PLOS One 2016;11 (11):e0165125.
190. Colagar AH, Marzony ET. Ascorbic acid in human seminal plasma: determination and its relationship to sperm quality. Journal of Clinical Biochemistry and Nutrition 2009;45(2): 144-9.
191. Colagar AH, Marzony ET, Chaichi MJ. Zinc levels in seminal plasma are associated with sperm quality in fertile and infertile men. Nutrition Research 2009; Vol. 29, issue 2:82-8.
192. Collins JA, Barnhart KT, Schlegel PN. Do sperm DNA integrity tests predict pregnancy with in vitro fertilization?. Fertility and Sterility 2008;89(4):823-31.
193. Collodel G, Federico MG, Geminiani M, Martini S, Bonechi C, Rossi C, et al. Effect of trans-resveratrol on induced oxidative stress in human sperm and in rat germinal cells. Reproductive Toxicology 2011;31(2):239-46.
194. Colone M, Marelli G, Unfer V, Bozzuto G, Molinari A, Stringaro A. Inositol activity in oligoasthenoteratospermia--an in vitro study. European Review for Medical and Pharmacological Sciences 2010;14(10):891-6. [1128-3602: (Print)]
195. Comhaire FH. Towards more objectivity in diagnosis and management of male infertility. International Journal of Andrology. Supplement. Oxford; Melbourne: Blackwell Scientific, 1987; Vol. 7.
196. Condorelli RA, La Vignera S, Mongioi LM, Vitale SG, Lagana AS, Cimino L, et al. Myo-inositol as a male fertility molecule: speed them up!. European Review for Medical and Pharmacological Sciences 2017;21(2 Suppl):30-5. [2284-0729: (Electronic)]
197. Dattilo M, Giuseppe D, Ettore C, Ménézo Y. Improvement of gamete quality by stimulating and feeding the endogenous antioxidant system: mechanisms, clinical results, insights on gene-environment interactions and the role of diet. Journal of Assisted Reproduction and Genetics 2016;33(12):1633-48.
198. de Angelis C, Galdiero M, Pivonello C, Garifalos F, Menafra D, Cariati F, et al. The role of vitamin D in male fertility: A focus on the testis. Reviews in Endocrine and Metabolic Disorders 2017;18(3):285-305.
199. Dias S, McNamee R, Vail A. Evidence of improving quality of reporting of randomised controlled trials in subfertility. Human Reproduction 2006;21(10):2617-27.
200. Ebisch IM, Thomas CM Peters WH, Braat DD, Steegers-Theunissen RP. The importance of folate, zinc and antioxidants in the pathogenesis and prevention of subfertility. Human Reproduction Update 2007;13(2): 163-74.
201. El-Taieb MA, Herwig R, Nada EA, Greilberger J, Marberger M. Oxidative stress and epididymal sperm transport, motility and morphological defects. European Journal of Obstetrics, Gynecology, and Reproductive Biology 2009;144 Suppl 1:199-203.
202. The European Society of Human Reproduction and Embryology (ESHRE). Guidelines to the prevalence, diagnosis, treatment and management of infertility, 1996. Human Reproduction 1996;11(8):1775.
203. Eskanazi B, Kidd SA, Marks AR, Sloter E, Block G, Wyrobeck AJ. Antioxidant intake is associated with semen quality in healthy men. Human Reproduction 2005;20(4): 1006-12.
204. Evenson DP, Jost LK, Marshall D, Zinaman MJ, Clegg E, Purvis K, et al. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Human Reproduction 1999;14(4):1039-49.
205. Evenson D, Wixon R. Meta-analysis of sperm DNA fragmentation using the sperm chromatin structure assay. Reproductive Biomedicine Online 2006;12(4):466-72.
206. Evers JL. Female subfertility. Lancet 2002;360(9327): 151-9. [0140-6736: (Print)]
207. Garg H, Kumar R. An update on the role of medical treatment including antioxidant therapy in varicocele. Asian Journal of Andrology 2016;18(2):222-8.
208. Ghyczy M, Boros M. Electrophilic methyl groups present in the diet ameliorate pathological states induced by reductive and oxidative stress: a hypothesis. British Journal of Nutrition 2001;85(4):409-14.
209. Giordano E, Visioli F. Long-chain omega 3 fatty acids: Molecular bases of potential antioxidant actions. Prostaglandins Leukotrienes and Essential Fatty Acids 2014;90 (1):1-4.
210. Gnoth C, Godehardt E, Frank-Herrmann P, Friol K, Tigges J, Freundl G. Definition and prevalence of subfertility and infertility. Human Reproduction 2005;20(5):1144-7.
211. GRADEpro. GRADEpro Guideline Development Tool. McMaster University: developed by Evidence Prime, Inc., 2015.
212. Grand View Research. Dietary Supplements Market Analysis By Ingredient (Botanicals, Vitamins, Minerals, Amino Acids, Enzymes), By Product (Tablets, Capsules, Powder, Liquids, Soft Gels, Gel Caps), By Application (Additional Supplement, Medicinal Supplement, Sports Nutrition), By End-Use (Infant, Children, Adults, Pregnant Women, Old-Aged) And Segment Forecasts To 2024. https://www.grandviewresearch.com 2016:0-200.
213. Grune T, Lietz G, Palou A, Ross AC, Stahl W, Tang G, et al. β-Carotene Is an Important Vitamin A Source for Humans1-3. Journal of Nutrition 2010;140(12): 2268S-85S.
214. Halicka HD, Zhao H, Li J, Traganos F, Studzinski GP, Darzynkiewicz Z. Attenuation of constitutive DNA damage signaling by 1,25-dihydroxyvitamin D3. Aging. Impact Journals, LLC, 2012; Vol. 4, issue 4:270-8.
215. Hankey GJ, Eikelboom JW. Homocysteine and vascular disease. Lancet 1999; Vol. 354, issue 9176:407-13.
216. Hausenblas HA, Schoulda JA, Smoliga JM. Resveratrol treatment as an adjunct to pharmacological management in type 2 diabetes mellitus-systematic review and meta-analysis. Molecular Nutrition & Food Research 2014;59(1): 147-59.
217. Higgins JP, Green S, editors. Cochrane Handbook for Systematic Reviews of Interventions 5.1.0 (updated March 2011). The Cochrane Collaboration 2011. Available from www.cochrane-handbook.org. Chichester UK: John Wiley and Sons Ltd.
218. Hsia T, Yin M. Post-intake of S-Ethyl Cysteine and S-Methyl Cysteine improved LPS-induced acute lung injury in mice. Nutrients 2016;8(8):507.
219. Huang D, Tocmo R. Assays based on competitive measurement of the scavenging ability of reactive oxygen/ nitrogen species. In: Apak R, Capanoglu E, Shahidi F editor(s). Measurement of Antioxidant Activity and Capacity: Recent Trends and Applications. Chichester, West Sussex, UK: John Wiley & Sons Ltd, 2018.
220. Hughes CM, Lewis SE, McKelvey-Martin VJ, Thompson W. A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay. Molecular Human Reproduction 1996;2(8):613-9. [1360-9947: (Print)]
221. Inhorn MC, Patrizio P. Infertility around the globe: new thinking on gender, reproductive technologies and global movements in the 21st century. Human Reproduction Update 2015;21(4):411-26. [1460-2369: (Electronic)]
222. Institute of Medicine. Vitamin E. Dietary Reference Intakes for Vitamin C, Vitamin E, Selenium, and Carotenoids. Washington, DC: The National Academies Press, 2000.
223. Intasqui P, Antoniassi M, Camargo M, Nichi M, Carvalho VM, Cardozo KHM, et al. Differences in the seminal plasma proteome are associated with oxidative stress levels in men with normal semen parameters. Fertility and Sterility 2015;104(2):292-301.
224. Irani M, Amirian M, Sadeghi R, Lez JL, Latifnejad Roudsari R. The effect of folate and folate plus zinc supplementation on endocrine parameters and sperm characteristics in subfertile men: a systematic review and meta-analysis. Urology Journal August 29 2017;14(5):4069-78.
225. Irvine DS. Epidemiology and aetiology of male infertility. Human Reproduction 1998;13 Suppl 1:33-44. [0268-1161: (Print)]
226. Iwasaki A, Gagnon C. Formation of reactive oxygen species in spermatozoa of infertile patients. Fertility and Sterility 1992;57(2):409-16. [0015-0282: (Print)]
227. Jones R, Mann T. Lipid peroxidation in spermatozoa. Proceedings of the Royal Society Series B-Biological Sciences 1973;184(1074):103-7. [0080-4649]
228. Joshi R, Adhikari S, Patro BS, Chattopadhyay S, Mukherjee T. Free radical scavenging behavior of folic acid: evidence for possible antioxidant activity. Free Radical Biology & Medicine 2001;30(12):1390-9.
229. Kodama H, Yamaguchi R, Fukuda J, Kasai H, Tanaka T. Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertilty and Sterility 1997;68(3):519-24. [0015-0282: (Print)]
230. Kofi Arhin S, Zhao Y, Lu XS, Chetry M, Lu JQ. Effect of micronutrient supplementation on IVF outcomes: a systematic review of the literature. Reproductive BioMedicine Online 2017;35(6):715-22.
231. Lafuente R, Gonzalez-Comadran M, Sola I, Lopez G, Brassesco M, Carreras R, et al. Coenzyme Q10 and male infertility: a meta-analysis. Journal of Assisted Reproduction and Genetics. 2013;30(9):1147-56.
232. Lewin LM, Yannai Y, Sulimovici S, Kraicer PF. Studies on the metabolic role of myo-inositol. Distribution of radioactive myo-inositol in the male rat. Biochemical Journal 1976;156(2):375-80.
233. Lewis SE, Sterling ES, Young IS, Thompson W. Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile men. Fertility and Sterility 1997;67(1): 142-7.
234. Lewis SEM, Aitken RJ, Conner SJ, Iuliis GD, Evenson DP, Henkel R, et al. The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment. Reproductive BioMedicine Online 2013; Vol. 27, issue 4:325-37.
235. Li Z, Wang L, Cai J, Huang H. Correlation of sperm DNA damage with IVF and ICSI outcomes: a systematic review and meta-analysis. Journal of Assisted Reproduction and Genetics 2006;23(9-10):367-76.
236. Littarru GP, Tiano L. Bioenergetic and antioxidant properties of coenzyme Q10: recent developments. Molecular Biotechnology 2007;37(1):31-7.
237. MacFarquhar JK, Broussard DL, Melstrom P, Hutchinson R, Wolkin A, Martin C, et al. Acute selenium toxicity associated with a dietary supplement. Archives of Internal Medicine 2010;170(3):256-61.
238. Majzoub A, Agarwal A. Antioxidant therapy in idiopathic oligoasthenoteratozoospermia. Indian Journal of Urology 2017;33(3):207-14.
239. Majzoub A, Agarwal A. Systematic review of antioxidant types and doses in male infertility: Benefits on semen parameters, advanced sperm function, assisted reproduction and live-birth rate. Arab Journal of Urology 2 January 2018; 16(1):113-24.
240. Mascarenhas MN, Flaxman SR, Boerma T, Vanderpoel S, Stevens GA. National, regional, and global trends in infertility prevalence since 1990: a systematic analysis of 277 health surveys. PLOS Medicine 2012;9(12):e1001356. [1549-1676: (Electronic)]
241. Mazzilli F, Rossi T, Marchesini M, Ronconi C, Dondero F. Superoxide anion in human semen related to seminal parameters and clinical aspects. Fertility and Sterility 1994; 62(4):862-8. [0015-0282: (Print)]
242. McNeill DA, Ali PS, Song YS. Mineral analyses of vegetarian, health, and conventional foods: magnesium, zinc, copper, and manganese content. Journal of American Dietetic Association 1985;85(5):569-72. [0002-8223: (Print)]
243. Mendiola J, Torres-Cantero AM, Vioque J, Moreno-Grau JM, Ten J, Roca M, et al. A low intake of antioxidant nutrients is associated with poor semen quality in patients attending fertility clinics. Fertility and Sterility 2010;93(4): 1128-33.
244. Miroczuk-Chodakowska I, Witkowska AM, Zujko ME. Endogenous non-enzymatic antioxidants in the human body. Advances in Medical Sciences 2018;63(1):68-78.
245. Navarro-Alarcon M, Cabrera-Vique C. Selenium in food and the human body: A review. Science of The Total Environment 2008;400(1):115-41.
246. Osman A, Alsomait H, Seshadri S, El-Toukhy T, Khalaf Y. The effect of sperm DNA fragmentation on live birth rate after IVF or ICSI: a systematic review and meta-analysis. Reproductive Biomedicine Online 2015;30(2):120-7.
247. Ourique GM, Finamor IA, Saccol EM, Riffel AP, Pês TS, Gutierrez K, et al. Resveratrol improves sperm motility, prevents lipid peroxidation and enhances antioxidant defences in the testes of hyperthyroid rats. Reproductive Toxicology 2013;37:31-9.
248. Padayatty SJ, Katz A, Wang Y, Eck P, Kwon O, Lee J, et al. Vitamin C as an antioxidant: evaluation of Its role in disease prevention. Journal of the American College of Nutrition 2003;22(1):18-35.
249. Pasqualotto FF, Sharma RK, Kobayashi H, Nelson DR, Jr AJT, Agarwal A. Oxidative stress in normospermic men undergoing infertility evaluation. Journal of Andrology 2001;22(2):316-22.
250. Patel SR, Sigman M. Antioxidant therapy in male infertility. Urologic Clinics of North America. 2008;35(2):319-30. MEDLINE: 18423251
251. Pravst I, mitek K, mitek J. Coenzyme Q10 contents in foods and fortification strategies. Critical Reviews in Food Science and Nutrition 2010;50(4):269-80.
252. Nordic Cochrane Centre, The Cochrane Collaboration. Review Manager 5 (RevMan 5). Version 5.3. Copenhagen: Nordic Cochrane Centre, The Cochrane Collaboration, 2014.
253. Richard D, Kefi K, Barbe U, Bausero P, Visioli F. Polyunsaturated fatty acids as antioxidants. Pharmacological Research 2008;57(6):451-5.
254. Robinson L, Gallos ID, Conner SJ, Rajkhowa M, Miller D, Lewis S, et al. The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis. [Review]. Human Reproduction 2012;27(10):2908-17.
255. Rogovik AL, Vohra S, Goldman RD. Safety considerations and potential interactions of vitamins: should vitamins be considered drugs?. Annals of Pharmacotherapy 2009;44(2): 311-24.
256. Ross AC. Vitamin A and Carotenoids. In: Shils ME SM, Ross AC, Caballero B, Cousins R editor(s). Modern Nutrition in Health and Disease. Vol. 10th edition, Lippincott Williams & Wilkins, 2006:351-75.
257. Ross C, Morriss A, Khairy M, Khalaf Y, Braude P, Coomarasamy A, El-Toukhy T. A systematic review of the effect of oral antioxidants on male infertility. Reproductive Biomedicine Online 2010;20(6):711-23. [PUBMED: 20378409]
258. Salas-Huetos A, Bulló M, Salas-Salvadó J. Dietary patterns, foods and nutrients in male fertility parameters and fecundability: a systematic review of observational studies. Human Reproduction Update 1 July 2017;23(4):371-89.
259. Shekarriz M, Thomas AJ Jr, Agarwal A. Incidence and level of seminal reactive oxygen species in normal men. Urology 1995;45(1):103-7. [0090-4295: (Print)]
260. Shimura T, Toyoshima M, Taga M, Shiraishi K, Uematsu N, Inoue M, et al. The novel surveillance mechanism of the Trp53-dependent s-phase checkpoint ensures chromosome damage repair and preimplantation-stage development of mouse embryos fertilized with x-irradiated sperm. Radiation Research 2002;158(6):735-42.
261. Showell MG, Mackenzie-Proctor R, Jordan V, Hart RJ. Antioxidants for female subfertility. Cochrane Database of Systematic Reviews 2017, Issue 7. DOI: 10.1002/ 14651858.CD007807.pub3
262. Sikka S, Rajasekaran M, Hellstrom W. Role of oxidative stress and antioxidants in male infertility. Journal of Andrology 1995;16(6):464-8.
263. Simon L, Murphy K, Shamsi MB, Liu L, Emery B, Aston KI, et al. Paternal influence of sperm DNA integrity on early embryonic development. Human Reproduction 2014; 29(11):2402-12.
264. Smith R, Vantman D, Ponce J, Escobar J, Lissi E. Total antioxidant capacity of human seminal plasma. Human Reproduction 1996;11(8):1655-60.
265. Spanò M, Bonde JP, Hjøllund HI, Kolstad HA, Cordelli E, Leter G. Sperm chromatin damage impairs human fertility. The Danish First Pregnancy Planner Study Team. Fertility and Sterility 2000;73(1):43-50.
266. Thonneau P, Marchand S, Tallec A, Ferial M, Ducot B, Lansac J, et al. Incidence and main causes of infertility in a resident population (1 850 000) of three French regions (1988-1989)*. Human Reproduction 1991;6(6):811-6.
267. Traber MG, Atkinson J. Vitamin E, antioxidant and nothing more. Free Radical Biology and Medicine 2007;43(1):4-15.
268. Tremellen K. Oxidative stress and male infertility - a clinical perspective. Human Reproduction Update 2008;14(3): 243-58.
269. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biological Interactions 2006;160(1):1-40.
270. Verit FF, Verit A, Kocyigit A, Ciftci H, Celik H, Koksal M. No increase in sperm DNA damage and seminal oxidative stress in patients with idiopathic infertility. Archives of Gynecology and Obstetrics 2006;274(6):339-44.
271. Wathes DC, Abayasekara DR, Aitken RJ. Polyunsaturated fatty acids in male and female reproduction. Biology of Reproduction 2007;77(2):190-201.
272. Winters BR, Walsh TJ. The epidemiology of male infertility. Urologic Clinics of North America 2014;41(1):195-204. [1558-318X: (Electronic)]
273. Yavuz Y, Mollaoglu H, Yurumez Y, Ucok K, Duran L, Tunay K, et al. Therapeutic effect of magnesium sulphate on carbon monoxide toxicity-mediated brain lipid peroxidation. European Review for Medical and Pharmacological Sciences 2013;17 Suppl 1:28-33. [1128-3602: (Print)]
274. Zareba P, Colaci DS, Afeiche M, Gaskins AJ, Jorgensen N, Mendiola J, et al. Semen quality in relation to antioxidant intake in a healthy male population. Fertility and Sterility 2013;100(6):1572-9.
275. Zegers-Hochschild F, Adamson GD, Dyer S, Racowsky C, de Mouzon J, Sokol R, et al. The International Glossary on Infertility and Fertility Care, 2017. Human Reproduction 2017;32(9):1786-1801. [1556-5653: (Electronic)]
276. Zhang Z, Zhu L, Jiang H, Chen H, Chen Y, Dai Y. Sperm DNA fragmentation index and pregnancy outcome after IVF or ICSI: a meta-analysis. Journal of Assisted Reproduction and Genetics 2015;32(1):17-26.
277. Zhao J, Huang X, Xu B, Yan Y, Zhang Q, Li Y. Whether vitamin D was associated with clinical outcome after IVF/ ICSI: a systematic review and meta-analysis. Reproductive Biology and Endocrinology 2018;16(1):13.
278. Zhou X, Liu F, Zhai S. Effect of L-carnitine and/or L-acetyl-carnitine in nutrition treatment for male infertility: a systematic review. Asia Pacific Journal of Clinical Nutrition 2007;16 Suppl 1:383-90. MEDLINE: 17392136
279. Zini A, de Lamirande E, Gagnon C. Reactive oxygen species in semen of infertile patients: levels of superoxide dismutase- and catalase-like activities in seminal plasma and spermatozoa. International Journal of Andrology 1993; Vol. 16, issue 3:183-8. [0105-6263: (Print)]
280. Zini A, Dohle G. Are varicoceles associated with increased deoxyribonucleic acid fragmentation? [Review]. Fertility and Sterility 2011;96(6):1283-7.
281. Showell MG, Brown J, Yazdani A, Stankiewicz MT, Hart RJ. Antioxidants for male subfertility. Cochrane Database of Systematic Reviews 2008, Issue 4. DOI: 10.1002/ 14651858.CD007411
282. Showell MG, Brown J, Yazdani A, Stankiewicz MT, Hart RJ. Antioxidants for male subfertility. Cochrane Database of Systematic Reviews 2011, Issue 1. DOI: 10.1002/ 14651858.CD007411.pub2
283. Showell MG, Mackenzie-Proctor R, Brown J, Yazdani A, Stankiewicz MT, Hart RJ. Antioxidants for male subfertility. Cochrane Database of Systematic Reviews 2014, Issue 12. DOI: 10.1002/14651858.CD007411.pub3