A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay

Molecular Human Reproduction

Volumn 2, Issue 8, 1996, Pages 613-619

  Hughes, Ciara M ^a   Lewis, Sheena E M ^a   McKelvey Martin, Valerie J ^b   Thompson, W ^a  

^a QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

^b UNIVERSITY OF ULSTER   (United Kingdom)

Author keywords

DNA damage; Human spermatozoa; Modified comet assay

Indexed keywords

DNA; HYDROGEN PEROXIDE;

AGAR GEL ELECTROPHORESIS; ARTICLE; COMPARATIVE STUDY; DNA DAMAGE; DNA REPAIR; DRUG EFFECT; HUMAN; IN VITRO STUDY; MALE; MALE INFERTILITY; METABOLISM; METHODOLOGY; RADIATION DOSE; RADIATION EXPOSURE; SPERMATOZOON;

DNA; DNA DAMAGE; DNA REPAIR; ELECTROPHORESIS, AGAR GEL; HUMANS; HYDROGEN PEROXIDE; INFERTILITY, MALE; MALE; RADIATION TOLERANCE; SPERMATOZOA;

EID: 0030202411     PISSN: 13609947     EISSN: None     Source Type: Journal    
DOI: 10.1093/molehr/2.8.613     Document Type: Review

References (49)

1. Aitken, J. and Fisher, H. (1994) Reactive oxygen species generation and human spermatozoa: the balance of benefit and risk. Bio Essays, 16, 259-267.
2. Ashwood-Smith, M.J. and Edwards, R.G. (1996) DNA repair by oocytes. Mol. Hum. Reprod., 2, 46-51.
3. Baker, H.W.G. (1993) Treatment of male infertility - what does the future hold? Int. J. Andorra, 16, 343-348.
4. Barren, J.G., De Lara, J., Cummings, K.B. and Ward, W. S. (1994) DNA organisation in human spermatozoa. J. Androl., 15, 139-144.
5. Biggers, J.D., Whitten, W.K., and Whittingham, D.G. (1971) The culture of mouse embryos in vitro. In David, J.C. (ed.), Methods in Mammalian Embryology. Freeman Press, San Francisco, pp. 86-94.
6. Brandreff, B.F., Gordan, L.A. and Carrano, A.V. (1990) Cytogenics of human sperm: structural aberrations and DNA replication. Prog. Clin. Biol. Res., 340B, 425-434.
7. Chandley, A.C. and Kofman-Algaro, S. (1971) Unscheduled DNA synthesis in human germ cells following UV irradiation. Exp. Cell Res., 69, 45-48.
8. Eng, E., Scholberg, A., Clausen, O.P.F. and Purves, K. (1993) DNA flow cytometry of sperm from normozoospermic men in barren couples and men of proven fertility. Int. J. Fertil., 38, 305-310.
9. Evenson, D.P., Jost, L.K., Baer, R.K. et al. (1991) Individuality of DNA denaturation patterns in human sperm as measured by the sperm chromatin structure assay. Reprod. Toxicol., 5, 115-125.
10. Fairbairn, D.W., Olive, P.L. and O'Neill, K.L. (1995) The Comet assay: a comprehensive review. Mutat. Res., 339, 37-59.
11. Hittmair, A., Rogatsch, H., Mikuz, G. and Feichtinger, H. (1994) Quantification of spermatogenesis by dual parameter flow cytometry. Fertil. Steril., 61, 746-750.
12. Irvine, D.A. (1992) Assessment of spermatogenesis. Curr. Obstet. Gynaecol., 2, 20-26.
13. Krausz, C., Mills, C., Rogers, S. et al. (1994) Stimulation of oxygen generation by human sperm suspensions using phorbol esters and formyl peptides: relationship with motility and fertilisation in vitro. Fertil. Stertil., 62, 599-605.
14. Kruger, T., Swanson, R., Acosta, A.A. et al. (1988) Abnormal sperm morphology and other sperm parameters related to the outcome of the hamster oocyte human sperm penetration assay. Int. J. Androl., 11, 107-113.
15. Kruger, T., Acosta, A.A., Simmons, K.F. et al. (1988) Predictive value of abnormal sperm morphology in in vitro fertilisation. Fertil. Steril., 49, 112-117.
16. Lee, S.E. and Choo, M.S. (1991) Flow cytometric analysis of testes in fertile men: a comparison of the ploidy to routine histopathological study. Eur. Urol., 20, 33-38.
17. Lewis, S.E.M., Boyle, P.M., McKinney, K.A. et al. (1995) Total antioxidant capacity of seminal plasma is different in fertile and infertile men. Fertil. Steril., 64, 866-870.
18. Lipitz, S., Bert, B., Radian, C. et al. (1992) Sperm head ultramorphology and chromatin stability of males with unexplained infertility who fail to fertilize normal human ova in vitro. Andrologia, 24, 261-269.
19. Liu, D.Y. and Baker, H.W.G. (1988) The proportion of human sperm with poor morphology, but normal intact acrosomes detected with pisum sativum agglutinin correlates with fertilisation in vitro. Fertil. Steril., 50, 288-293.
20. Liu, D.Y. and Baker, H.W.G. (1992) Sperm nuclear chromatin normality: relationship with sperm morphology, sperm zona-pellucida binding, and fertilisation rates in vitro. Fertil. Steril., 58, 1178-1184.
21. McKelvey-Martin, V.J., Green, M.H.L., Schmer, P. et al. (1993) The single cell gel electrophoresis assay (comet assay): a European review. Mut. Res., 288, 47-63.
22. McKelvey-Martin, V.J., Melia, N., Walsh, I.K. et al. (1996) Two potential clinical applications of the alkaline single cell gel electrophoresis assay. Mutat. Res. (in press)
23. McKinney, K.A., Lewis, S.E.M. and Thompson, W. (1995) Reactive oxygen species generation in human spermatozoa. A comparison of luminol and lucigen chemiluminescence probes. Arch. Androl. (in press).
24. Molina, J., Castilla, J.A., Gil, T. et al. (1995) Influence of incubation on the chromatin condensation and nuclear stability of human spermatozoa by flow cytometry. Mol. Hum. Reprod., 1,
25. see Hum. Reprod., 10, 1280-1286.
26. Oehinger, S., Acosta, A., Veek, L.C. et al. (1991) Recurrent failure of in vitro fertilisation: role of the hemizona assay in the sequential diagnosis of specific sperm oocyte defects. Am. J. Obstet. Gynecol., 164 (5 pt 1), 1210-1215.
27. Ostling, O. and Johanson, K.J. (1984) Microelectrophoretic study of radiation induced damages in individual mammalian cells. Biochem. Biophys. Res. Commun., 123, 291-298.
28. Palmero, G., Joris, H., Devroey, P. and Van Steirteghem, A.C. (1992) Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet, 340, 17-18.
29. Rosenbusch, B. and Sterzik, K. (1994) Sperm chromosomes and habitual abortion. Fertil. Steril., 56, 370-372.
30. Rousseaux, S. and Chevret, E. (1995) In-vitro decondensation of human spermatozoa for fluorescence in-situ hybridization. Mol. Hum. Reprod., 1,
31. see Hum. Reprod., 10, 2209-2213.
32. Rydbert, B. and Johanson, K.J. (1978) Estimations of DNA strand breaks in single mammalian cells. In Hanawalt, P.C., Freidberg, E.C. and Fox, C.F. (eds), DNA Repair Mechanisms. Academic Press, New York, pp. 465-468.
33. Sega, G.A. (1976) Molecular disometry of chemical mutagens: measurement of molecular dose and DNA repair in mammalian germ cells. Mutat. Res., 38, 317-326.
34. Sega, G.A. and Generoso, E. (1990) Measurement of DNA breakage in specific germ cell stages of male mice exposed to acrylamide, using an alkaline elution procedure. Mutat. Res., 242, 79-87.
35. Sega, G.A., Sluder, A.E., McCoy, L.S. et al. (1986) The use of alkaline elution procedures to measure DNA damage in spermatogenic stages of mice exposed to methansulfonate. Mutat. Res., 159, 55-63.
36. Sidney, R. and Grimes, J.R. (1986) Nuclear proteins in spermatogenesis. Comp. Biochem. Physiol., 83, 495-500.
37. Singh, N.P., McCoy, M.T., Tice, R.R. and Schneider, E.L. (1988) A simple technique forquantitation of low levels of DNA damage in individual cells. Exp. Cell. Res., 175, 184-191.
38. Singh, N.P., Danner, D.B., Tice, R.R. et al. (1989) Abundant alkali sensitive sites in DNA of human and mouse sperm. Exp. Cell. Res., 184, 461-470.
39. Spano, M and Evenson, D.P. (1991) Flow cytometric studies in reproductive toxicology. In New Horizons in Biological Disymmetry. pp. 497-511.
40. Tejada, R.J., Mitchall, J.C., Norman, A. et al. (1984) A test for practical evaluation of male fertility by acridine orange (AO) fluorescence. Fertil. Steril., 42, 87-91.
41. Van Loon, A.A.W.M., Den Boer, P.J., Van der Schans, G.P. et al. (1991) Immunochemical detection of DNA damage induction and repair at different cellular stages of spermatogenesis of the hamster after in vitro or m vivo exposure to ionising radiation. Exp. Cell Res., 193, 303-309.
42. Van Loon, A.A.W.M., Sonneveld, E., Hoogerbrugge, J. et al. (1993) Induction and repair of single strand breaks and DNA base damage at different cellular stages of spermatogenesis of the hamster upon in vitro exposure to ionising radiation. Mutat. Res., 294, 139-148.
43. Van Steirteghem, A., Liu, J., Joris, H. et al. (1994) Assisted fertilisation by subzonal insemination and intracytoplasmic sperm injection. Reprod. Fertil. Dev., 6, 85-91.
44. Ward, W.S. (1993) DNA loop domain tertiary structure in mammalian spermatozoa. Biol. Reprod., 48, 1193-1201.
45. Ward, W.W. and Coffey, D.S. (1991) DNA packaging and organisation in mammalian spermatogenesis: comparison with somatic cells. Biol. Reprod., 44, 569-574.
46. Wiger, R., Bjorge, C., Holme, J.A. et al. (1995) Chemically induced single strand breaks and their repair in spermatogenic cells. Environ. Mutag. Hum. Popul., p. 66.
47. World Health Organisation (1992) Laboratory Manual for the Examination of Semen and Sperm-Cervical Mucus Interaction. Cambridge University Press, Cambridge, p. 107.
48. Zamboni, L. (1992) Sperm structure and its relevance to infertility. Arch. Pathol. Lab. Med., 116, 325-344.
49. Zirkin, B., Soucek, D., Chang, T. S. K. and Perreault, S. D. (1985) In vitro and in vivo studies of mammalian sperm nuclear decondensation. Gamete Res., 11, 349-365.