Ecology of listeria species and L. monocytogenes in the natural environment

Listeria, Listeriosis, and Food Safety, Third Edition

Volumn , Issue , 2007, Pages 21-54

  Sauders, Brian D ^a   Wiedmann, Martin ^a  

^a Department of Obstetrics Gynecology   (United States)

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EID: 85048045722     PISSN: None     EISSN: None     Source Type: Book    
DOI: None     Document Type: Chapter

References (213)

1. Aarnisalo, K., T. Autio, A. M. Sjoberg, J. Lunden, H. Korkeala, and M. L. Suihko. 2003. Typing of Listeria monocytogenes isolates originating from the food processing industry with automated ribotyping and pulsed-field gel electrophoresis. J. Food Prot. 66:249-255.
2. Adams, C. J., T. E. Neff, and L. L. Jackson. 1979. Induction of Listeria monocytogenes infection by the consumption of ponderosa pine needles. Infect. Immun. 25:117-120.
3. al-Ghazali, M. R., and S. K. al-Azawi. 1988. Effects of sewage treatment on the removal of Listeria monocytogenes. J. Appl. Bacteriol. 65:203-208.
4. al-Ghazali, M. R., and S. K. al-Azawi. 1988. Storage effects of sewage sludge cake on the survival of Listeria monocytogenes. J. Appl. Bacteriol. 65:209-213.
5. Amtsberg, G. von. 1979. Epidemiology and diagnosis of Listeria infections. 1. Dtsch. Tierärztl. Wochenschr. 86:253-257.
6. Anonymous. 1999. Update: Multistate outbreak of listeriosis-United States, 1998-1999. MMWR 47:1117-1118.
7. Anonymous. 2000. Multistate outbreak of listeriosis-United States, 2000. MMWR 49:1129-1130.
8. Anonymous. 2002. Outbreak of listeriosis-northeastern United States, 2002. MMWR 51:950-951.
9. Antoniollo, P. C., S. Bandeira Fda, M. M. Jantzen, E. H. Duval, and W. P. da Silva. 2003. Prevalence of Listeria spp. in feces and carcasses at a lamb packing plant in Brazil. J. Food Prot. 66:328-330.
10. Arimi, S. M., E. T. Ryser, T. J. Pritchard, and C. W. Donnelly. 1997. Diversity of Listeria ribotypes recovered from dairy cattle, silage, and dairy processing environments. J. Food Prot. 60:811-816.
11. Armstrong, D. 1985. Listeria monocytogenes. In Principles and practices of infectious diseases. 2nd ed. eds. G. L. Mandell, R. G. Douglas, Jamie Robertson, and J. E. Bennett, New York: Wiley.
12. Arvanitidou, M., A. Papa, T. C. Constantinidis, V. Danielides, and V. Katsouyannopoulos. 1997. The occurrence of Listeria spp. and Salmonella spp. in surface waters. Microbiol. Res. 152:395-397.
13. Autio, T., J. Lunden, M. Fredriksson-Ahomaa, J. Bjorkroth, A. M. Sjoberg, and H. Korkeala. 2002. Similar Listeria monocytogenes pulsotypes detected in several foods originating from different sources. Int. J. Food Microbiol. 77:83-90.
14. Barker, J., and M. R. Brown. 1994. Trojan horses of the microbial world: protozoa and the survival of bacterial pathogens in the environment. Microbiology 170:1253-1259.
15. Barnes, R., P. Archer, J. Strack, and G. R. Istre. 1989. Listeriosis associated with consumption of turkey franks. MMWR 38:267-268.
16. Basher, H. A., D. R. Fowler, F. G. Rodgers, A. Seaman, and M. Woodbine. 1984. Pathogenicity of natural and experimental listeriosis in newly hatched chicks. Res. Vet. Sci. 36:76-80.
17. Bauwens, L., F. Vercammen, and A. Hertsens. 2003. Detection of pathogenic Listeria spp. in zoo animal feces: use of immunomagnetic separation and a chromogenic isolation medium. Vet. Microbiol. 91:115-123.
18. Baxter, F., F. Wright, R. M. Chalmers, J. C. Low, and W. Donachie. 1993. Characterization by multilocus enzyme electrophoresis of Listeria monocytogenes isolates involved in ovine listeriosis outbreaks in Scotland from 1989 to 1991. Appl. Environ. Microbiol. 59:3126-3129.
19. Begley, M., C. G. Gahan, and C. Hill. 2002. Bile stress response in Listeria monocytogenes LO28:adaptation, cross-protection, and identification of genetic loci involved in bile resistance. Appl. Environ. Microbiol. 68:6005-6012.
20. Belding, R. C., and M. L. Mayer. 1959. Listeriosis in the turkey-two case reports. J. Am. Vet. Med. Assoc. 131:296-297.
21. Bernagozzi, M., F. Bianucci, R. Sacchetti, and P. Bisbini. 1994. Study of the prevalence of Listeria spp. in surface water. Zentralbl. Hyg. Umweltmed. 196:237-244.
22. Besnard, V., M. Federighi, E. Declerq, F. Jugiau, and J. M. Cappelier. 2002. Environmental and physico-chemical factors induce VBNC state in Listeria monocytogenes. Vet. Res. 33:359-370.
23. Bille, J. 1990. Epidemiology of human listeriosis in Europe, with special reference to the Swiss outbreak. In Foodborne listeriosis, ed. A. J. Miller, J. L. Smith, and G. A. Somkuti. New York:Elsevier.
24. Bille, J., J. Rocourt, and B. Swaminathan. 1999. Listeria, Erysipelothrix, and Kurthia. In Manual of clinical microbiology, 7th ed. American Society for Microbiology, Washington, D.C.
25. Boerlin, P., and J. C. Piffaretti. 1991. Typing of human, animal, food, and environmental isolates of Listeria monocytogenes by multilocus enzyme electrophoresis. Appl. Environ. Microbiol. 57:1624-1629.
26. Botzler, R. G., A. B. Cowan, and T. F. Wetzler. 1974. Survival of Listeria monocytogenes in soil and water. J. Wildl. Dis. 10:204-212.
27. Botzler, R. G., T. F. Wetzler, and A. B. Cowan. 1973. Listeria in aquatic animals. J. Wildl. Dis. 9:163-170.
28. Brosch, R., B. Catimel, G. Millon, C. Buchrieser, E. Vindel, and J. Rocourt. 1993. Virulence heterogeneity of Listeria monocytogenes strains from various sources (food, human, animal) in immunocompetent mice and its association with typing characteristics. J. Food Prot. 56:296-301.
29. Buchrieser, C., R. Brosch, B. Catimel, and J. Rocourt. 1993. Pulsed-field gel electrophoresis applied forcomparing Listeria monocytogenes strains involved in outbreaks. Can. J. Microbiol. 39:395-401.
30. Buchrieser, C., C. Rusniok, F. Kunst, P. Cossart, and P. Glaser. 2003. Comparison of the genome sequences of Listeria monocytogenes and Listeria innocua: Clues for evolution and pathogenicity. FEMS Immunol. Med. Microbiol. 35:207-213.
31. Cai, S., D. Y. Kabuki, A. Y. Kuaye, T. G. Cargioli, M. S. Chung, R. Nielsen, and M. Wiedmann. 2002. Rational design of DNA sequence-based strategies for subtyping Listeria monocytogenes. J. Clin. Microbiol. 40:3319-3325.
32. Capita, R., C. Alonso-Calleja, B. Moreno, and M. C. Garcia-Fernandez. 2001. Occurrence of Listeria species in retail poultry meat andcomparison of a cultural/immunoassay for their detection. Int. J. Food Microbiol. 65:75-82.
33. Caro, M. R., E. Zamora, L. Leon, F. Cuello, J. Salinas, D. Megias, M. J. Cubero, and A. Contreras. Isolation and identification of Listeria monocytogenes in vegetable byproduct silages containing preservative additives and destined for animal feeding. Anim. Feed Sci. Technol. 31:285-291.
34. Chakraborty, T., F. Ebel, J. Wehland, J. Dufrenne, and S. Notermans. 1994. Naturally occurring virulence-attenuated isolates of Listeria monocytogenes capable of inducing long-term protection against infection by virulent strains of homologous and heterologous serotypes. FEMS Immunol. Med. Microbiol. 10:1-9.
35. Colburn, K. G., C. A. Kaysner, C. Abeyta, Jr., and M. M. Wekell. 1990. Listeria species in a California coast estuarine environment. Appl. Environ. Microbiol. 56:2007-2011.
36. Conte, M. P., C. Longhi, M. Polidoro, G. Petrone, V. Buonfiglio, S. Di Santo, E. Papi, L. Seganti, P. Visca, and P. Valenti. 1996. Iron availability affects entry of Listeria monocytogenes into the enterocytelike cell line Caco-2. Infect. Immun. 64:3925-3929.
37. Cook, L. V. 2003. Isolation and identification of Listeria monocytogenes from red meat, poultry, egg, and environmental samples. United States Department of Agriculture. http://www.fsis.usda.gov/OPHS/microlab/mlgbook.htm (accessed 3/15/03).
38. Cox, L. J., T. Kleiss, J. L. Cordier, C. Cordellana, P. Konkel, C. Pedrazzini, R. Beumer, and A. Siebenga. 1989. Listeria spp. in food processing, nonfood and domestic environments. Food Microbiol. 6:49-61.
39. Cranfield, M., M. A. Eckhaus, B. A. Valentine, and J. D. Strandberg. 1985. Listeriosis in Angolan giraffes. J. Am. Vet. Med. Assoc. 187:1238-1240.
40. Dahllof, I. 2002. Molecularcommunity analysis of microbial diversity. Curr. Opin. Biotechnol. 13:213-217.
41. Dalton, C. B., C. C. Austin, J. Sobel, P. S. Hayes, W. F. Bibb, L. M. Graves, B. Swaminathan, M. E. Proctor, and P. M. Griffin. 1997. An outbreak of gastroenteritis and fever due to Listeria monocytogenes in milk. N. Engl. J. Med. 336:100-105.
42. De Luca, G., F. Zanetti, P. Fateh-Moghadm, and S. Stampi. 1998. Occurrence of Listeria monocytogenes in sewage sludge. Zentralbl. Hyg. Umweltmed. 201:269-277.
43. DeLong, E. F. 2002. Microbial population genomics and ecology. Curr. Opin. Microbiol. 5:520-524.
44. Destro, M. T., M. F. Leitao, and J. M. Farber. 1996. Use of molecular typing methods to trace the dissemination of Listeria monocytogenes in a shrimp processing plant. Appl. Environ. Microbiol. 62:705-711.
45. Dijkstra, R. G. 1971. Investigations on the survival times of Listeria bacteria in suspensions of brain tissue, silage and faeces and in milk. Zentralbl. Bakteriol. [Orig]. 216:92-95.
46. Dijkstra, R. G. 1975. The occurrence of Listeria encephalitis in cattle in a loose housing after the use of litter, infected with Listeria bacteria, from a broiler farm. To what extent do Listeria bacteria occur in the gut contents of broilers (author’s transl.)? Tijdschr. Diergeneeskd. 100:1154-1155.
47. Dijkstra, R. G. 1979. Listeria monocytogenes in intestinal contents and faeces from healthy broilers of different ages, in the litter and its potential danger for other animals including cattle. Proceedings of VII international symposium of problems in listeriosis, National Agroindustrial Union, Center for Scientific Information, Sofia, pp. 289-294.
48. Dijkstra, R. G. 1982. The occurrence of Listeria monocytogenes in surface water of canals and lakes, in ditches of one big polder and in the effluents and canals of a sewage treatment plant. Zentralbl. Bakteriol. Mikrobiol. Hyg. [B]. 176:202-205.
49. Dingle, K. E., F. M. Colles, D. R. Wareing, R. Ure, A. J. Fox, F. E. Bolton, H. J. Bootsma, R. J. Willems, R. Urwin, and M. C. Maiden. 2001. Multilocus sequence typing system for Campylobacter jejuni. J. Clin. Microbiol. 39:14-23.
50. Donachie, W., J. C. Low, F. Baxter, and F. Thompson-Carter. 1995. Typing of Listeria monocytogenes isolates with multilocus enzyme electrophoresis (MEE) and pulsed field gel electrophoresis (PFGE). Proceedings of XII international symposium on problems of listeriosis, Perth, Western Australia, Promaco Conventions Pty, Ltd., pp. 417-420.
51. Donald, A. S., D. R. Fenlon, and B. Seddon. 1995. The relationship between ecophysiology, indigenous microflora and growth of Listeria monocytogenes in grass silage. J. Appl. Bacteriol. 79:141-148.
52. Donnelly, C. W. 2002. Detection and isolation of Listeria monocytogenes from food samples: implications of sublethal injury. J. AOAC Int. 85:495-500.
53. Dutta, P. K., and B. S. Malik. 1981. Isolation and characterization of Listeria monocytogenes from animals and human beings. Indian J. Anim. Sci. 51:1045-1052.
54. Enright, M. C., K. Knox, D. Griffiths, D. W. Crook, and B. G. Spratt. 2000. Molecular typing of bacteria directly from cerebrospinal fluid. Eur. J. Clin. Microbiol. Infect. Dis. 19:627-630.
55. Enright, M. C., and B. G. Spratt. 1999. Multilocus sequence typing. Trends Microbiol. 7:482-487.
56. Fantelli, K., and R. Stephan. 2001. Prevalence and characteristics of shigatoxin-producing Escherichia coli and Listeria monocytogenes strains isolated from minced meat in Switzerland. Int. J. Food Microbiol. 70:63-69.
57. Farber, J. M., E. M. Daley, M. T. MacKie, and B. Limerick. 2000. A small outbreak of listeriosis potentially linked to the consumption of imitation crab meat. Lett. Appl. Microbiol. 31:100-104.
58. Farber, J. M., G. W. Sanders, and M. A. Johnston. 1989. A survey of various foods for the presence of Listeria species. J. Food Prot. 52:456-458.
59. Fenlon, D. R. 1985. Wild birds and silage as reservoirs of Listeria in the agricultural environment. J. Appl. Bacteriol. 59:537-543.
60. Fenlon, D.R. 1986. Growth of naturally occurring Listeria spp. in silage: Acomparative study of laboratory and farm ensiled grass. Grass Forage Sci. 41:375-378.
61. Fenlon, D. R. 1986. Rapid quantitative assessment of the distribution of Listeria in silage implicated in a suspected outbreak of listeriosis in calves. Vet. Rec. 118:240-242.
62. Fenlon, D. R. 1988. Listeriosis. In Silage and health, 1st ed., ed. B. A. Stark and J. M. Wilkinson. Marlow, England: Chalcombe Publications.
63. Fenlon, D. R., T. Stewart, and W. Donachie. 1995. The incidence, numbers and types of Listeria monocytogenes isolated from farm bulk tank milks. Lett. Appl. Microbiol. 20:57-60.
64. Fenlon, D. R., J. Wilson, and W. Donachie. 1996. The incidence and level of Listeria monocytogenes contamination of food sources at primary production and initial processing. J. Appl. Bacteriol. 81:641-650.
65. Fenlon, D. R., and J. R. Wilson. 1996. Enterobacteria as indicators of poor fermentation and Listeria contamination of silage. Proceedings of XI international silage conference, IGER, Aberystwyth, U.K., p. 103.
66. Fensterbank, R., A. Audurier, J. Godu, P. Guerrault, and N. Malo. 1984. Listeria strains isolated from sick animals and consumed silage. Ann. Rech. Vet. 15:113-118.
67. Finley, G. G., and J. R. Long. 1977. An epizootic of listeriosis in chinchillas. Can. Vet. J. 18:164-167.
68. Frances, N., H. Hornby, and P. R. Hunter. 1991. The isolation of Listeria species from fresh-water sites in Cheshire and North Wales. Epidemiol. Infect. 107:235-238.
69. Francis, M. S., and C. J. Thomas. 1996. Effect of multiplicity of infection on Listeria monocytogenes pathogenicity for HeLa and Caco-2 cell lines. J. Med. Microbiol. 45:323-330.
70. Frye, D. M., R. Zweig, J. Sturgeon, M. Tormey, M. LeCavalier, I. Lee, L. Lawani, and L. Mascola. 2002. An outbreak of febrile gastroenteritis associated with delicatessen meat contaminated with Listeria monocytogenes. Clin. Infect. Dis. 35:943-949.
71. Garcia, E., M. De Paz, J. L. Rodriguez, P. Gaya, M. Medina, and M. Nunez. 1996. Exogenous sources of Listeria contamination in raw ewe’s milk. J. Food Prot. 59:950-954.
72. Garrec, N., F. Picard-Bonnaud, and A. M. Pourcher. 2003. Occurrence of Listeria sp. and L. monocytogenes in sewage sludge used for land application: Effect of dewatering, liming and storage in tank on survival of Listeria species. FEMS Immunol. Med. Microbiol. 35:275-283.
73. Geuenich, H. H., and H. E. Muller. 1984. Isolation and germ count of Listeria monocytogenes in raw and biologically treated waste water. Zentralbl. Bakteriol. Mikrobiol. Hyg. (B). 179:266-273.
74. Gilbert, R. J., J. McLauchlin, and S. K. Velani. 1993. The contamination of pate by Listeria monocytogenes in England and Wales in 1989 and 1990. Epidemiol. Infect. 110:543-551.
75. Gitter, M. 1986. A changing pattern of ovine listeriosis in Great Britain. Proceedings of IX international symposium on problems of listeriosis, University of Nantes, Nantes, France, pp. 294-299.
76. Gitter, M., R. S. Stebbings, J. A. Morris, D. Hannam, and C. Harris. 1986. Relationship between ovine listeriosis and silage feeding. Vet. Rec. 118:207-208.
77. Glaser, P., L. Frangeul, C. Buchrieser, C. Rusniok, A. Amend, F. Baquero, P. Berche, H. Bloecker, P. Brandt, T. Chakraborty, A. Charbit, F. Chetouani, E. Couve, A. de Daruvar, P. Dehoux, E. Domann, G. Dominguez-Bernal, E. Duchaud, L. Durant, O. Dussurget, K. D. Entian, H. Fsihi, F. G. Portillo, P. Garrido, L. Gautier, W. Goebel, N. Gomez-Lopez, T. Hain, J. Hauf, D. Jackson, L. M. Jones, U. Kaerst, J. Kreft, M. Kuhn, F. Kunst, G. Kurapkat, E. Madueno, A. Maitournam, J. M. Vicente, E. Ng, H. Nedjari, G. Nordsiek, S. Novella, B. de Pablos, J. C. Perez-Diaz, R. Purcell, B. Remmel, M. Rose, T. Schlueter, N. Simoes, A. Tierrez, J. A. Vazquez-Boland, H. Voss, J. Wehland, and P. Cossart. 2001. Comparative genomics of Listeria species. Science 294:849-852.
78. Gombas, D. E., Y. Chen, R. S. Clavero, and V. N. Scott. 2003. Survey of Listeria monocytogenes in ready-to-eat foods. J. Food Prot. 66:559-569.
79. Gouin, E., J. Mengaud, and P. Cossart. 1994. The virulence gene cluster of Listeria monocytogenes is also present in Listeria ivanovii, an animal pathogen, and Listeria seeligeri, a nonpathogenic species. Infect. Immun. 62:3550-3553.
80. Graves, L. M., B. Swaminathan, and S. B. Hunter. 1999. Subtyping of Listeria monocytogenes. In Listeria, listeriosis, and food safety. 2nd ed. eds. E. T. Ryser and E. H. Marth, New York: Marcel Dekker, Inc., pp. 279-297.
81. Graves, L. M., B. Swaminathan, M. W. Reeves, S. B. Hunter, R. E. Weaver, B. D. Plikaytis, and A. Schuchat. 1994. Comparison of ribotyping and multilocus enzyme electrophoresis for subtyping of Listeria monocytogenes isolates. J. Clin. Microbiol. 32:2936-2943.
82. Gray, M. L. 1958. Listeriosis in fowls-a review. Avian Dis. 2:296-314.
83. Gray, M. L. 1960. Silage feeding and listeriosis. J. Am. Vet. Med. Assoc. 136:205-208.
84. Gray, M. L., and A. H. Killinger. 1966. Listeria monocytogenes and listeric infections. Bacteriol. Rev. 30:309-382.
85. Gray, M. L., C. Singh, and F. Thorp. 1956. Abortion and pre- or post-natal death of young due to Listeria monocytogenes. III. Studies in ruminants. Am. J. Vet. Res. 17:510-516.
86. Gray, M. L., H. J. Stafseth, F. Thorp, L. B. Scholl, and W. F. Riley. 1948. A new technique for isolating listerellae from the bovine brain. J. Bacteriol. 55:443-444.
87. Grif, K., I. Hein, M. Wagner, E. Brandl, O. Mpamugo, J. McLauchlin, M. P. Dierich, and F. Allerberger. 2001. Prevalence and characterization of Listeria monocytogenes in the feces of healthy Austrians. Wien. Klin. Wochenschr. 113:737-742.
88. Grif, K., G. Patscheider, M. P. Dierich, and F. Allerberger. 2003. Incidence of fecal carriage of Listeria monocytogenes in three healthy volunteers: A one-year prospective stool survey. Eur. J. Clin. Microbiol. Infect. Dis. 22:16-20.
89. Grønstol, H. 1979. Listeriosis in sheep. Isolation of Listeria monocytogenes from grass silage. Acta Vet. Scand. 20:492-497.
90. Harvey, J., and A. Gilmour. 1994. Application of multilocus enzyme electrophoresis and restriction fragment length polymorphism analysis to the typing of Listeria monocytogenes strains isolated from raw milk, nondairy foods, and clinical and veterinary sources. Appl. Environ. Microbiol. 60:1547-1553.
91. Hatkin, J. M., W. E. Phillips, Jr., and G. A. Hurst. 1986. Isolation of Listeria monocytogenes from an eastern wild turkey. J. Wildl. Dis. 22:110-112.
92. Haydon, D. T., S. Cleaveland, L. H. Taylor, and M. K. Laurenson. 2002. Identifying reservoirs of infection: A conceptual and practical challenge. Emerg. Infect. Dis. 8:1468-1473.
93. Hayes, P. S., L. M. Graves, G. W. Ajello, B. Swaminathan, R. E. Weaver, J. D. Wenger, A. Schuchat, and C. V. Broome. 1991. Comparison of cold enrichment and U.S. Department of Agriculture methods for isolating Listeria monocytogenes from naturally contaminated foods. The Listeria Study Group. Appl. Environ. Microbiol. 57:2109-2113.
94. Hitchins, A. D., and R. C. Whiting. 2001. Food-borne Listeria monocytogenes risk assessment. Food Addit. Contam. 18:1108-1117.
95. Hofer, E. 1983. Bacteriologic and epidemiologic studies on the occurrence of Listeria monocytogenes in healthy cattle. Zentralbl. Bakteriol. Mikrobiol. Hyg. [A]. 256:175-183.
96. Hoffman, A. D., K. L. Gall, D. M. Norton, and M. Wiedmann. 2003. Listeria monocytogenes contamination patterns for the smoked fish processing environment and for raw fish. J. Food Prot. 66:52-60.
97. Hohne, K., B. Loose, and H. P. Seeliger. 1975. Isolation of Listeria monocytogenes in slaughter animals and bats of Togo (West Africa). Ann. Microbiol. (Paris). 126A:501-507.
98. Holah, J. T., J. H. Taylor, D. J. Dawson, and K. E. Hall. 2002. Biocide use in the food industry and the disinfectant resistance of persistent strains of Listeria monocytogenes and Escherichia coli. Symp. Ser. Soc. Appl. Microbiol. 111S-120S.
99. Huss, H. H., L. V. Jorgensen, and B. F. Vogel. 2000. Control options for Listeria monocytogenes in seafoods. Int. J. Food Microbiol. 62:267-274.
100. Husu, J. R. 1990. Epidemiological studies on the occurrence of Listeria monocytogenes in the feces of dairy cattle. Zentralbl. Veterinarmed. B. 37:276-282.
101. Husu, J. R., J. T. Beery, E. Nurmi, and M. P. Doyle. 1990. Fate of Listeria monocytogenes in orally dosed chicks. Int. J. Food Microbiol. 11:259-269.
102. Husu, J. R., J. T. Seppanen, S. K. Sivela, and A. L. Rauramaa. 1990. Contamination of raw milk by Listeria monocytogenes on dairy farms. Zentralbl. Veterinarmed. B. 37:268-275.
103. Husu, J. R., S. K. Sivela, and A. L. Rauramaa. 1990. Prevalence of Listeria species as related to chemical quality of farm-ensiled grass. Grass Forage Sci. 45:309-314.
104. Jacquet, C., B. Catimel, R. Brosch, C. Buchrieser, P. Dehaumont, V. Goulet, A. Lepoutre, P. Veit, and J. Rocourt. 1995. Investigations related to the epidemic strain involved in the French listeriosis outbreak in 1992. Appl. Environ. Microbiol. 61:2242-2246.
105. Jacquet, C., B. Catimel, V. Goulet, A. Lepoutre, P. Veit, P. Dehaumont, and J. Rocourt. 1995. Typing of Listeria monocytogenes during epidemiological investigations of the French listeriosis outbreaks in 1992, 1993, and 1995. Proceedings of XII international symposium on problems of listeriosis, Perth, Western Australia, Promaco Conventions, pp. 61-176.
106. Jacquet, C., J. Rocourt, and A. Reynaud. 1993. Study of Listeria monocytogenes contamination in a dairy plant and characterization of the strains isolated. Int. J. Food Microbiol. 20:13-22.
107. Jeffers, G. T., J. L. Bruce, P. L. McDonough, J. Scarlett, K. J. Boor, and M. Wiedmann. 2001. Comparative genetic characterization of Listeria monocytogenes isolates from human and animal listeriosis cases. Microbiology 147:1095-1104.
108. Jensen, L. B., Y. Agerso, and G. Sengelov. 2002. Presence of erm genes among macrolide-resistant Gram-positive bacteria isolated from Danish farm soil. Environ. Int. 28:487-491.
109. Johnson, G. C., C. W. Maddox, W. H. Fales, W. A. Wolff, R. F. Randle, J. A. Ramos, H. Schwartz, K. M. Heise, A. L. Baetz, I. V. Wesley, and D. E. Wagner. 1996. Epidemiologic evaluation of encephalitic listeriosis in goats. J. Am. Vet. Med. Assoc. 208:1695-1699.
110. Kathariou, S. 2002. Listeria monocytogenes virulence and pathogenicity, a food safety perspective. J. Food Prot. 65:1811-1829.
111. Kikuhara, H., M. Ogawa, H. Miyamoto, Y. Nikaido, and S. Yoshida. 1994. Intracellular multiplication of Legionella pneumophila in Tetrahymena thermophila. J. Uoeh. 16:263-275.
112. Larsen, H. E. 1984. Investigation on the epidemiology of listeriosis-the distribution of Listeria monocytogenes in environments in which clinical outbreaks have not been diagnosed. Nord. Vet. Med. 16:890-909.
113. Lawrence, L. M., and A. Gilmour. 1994. Incidence of Listeria spp. and Listeria monocytogenes in a poultry processing environment and in poultry products and their rapid confirmation by multiplex PCR. Appl. Environ. Microbiol. 60:4600-4604.
114. Lawrence, L. M., and A. Gilmour. 1995. Characterization of Listeria monocytogenes isolated from poultry products and from the poultry-processing environment by random amplification of polymorphic DNA and multilocus enzyme electrophoresis. Appl. Environ. Microbiol. 61:2139-2144.
115. Le Marc, Y., V. Huchet, C. M. Bourgeois, J. P. Guyonnet, P. Mafart, and D. Thuault. 2002. Modeling the growth kinetics of Listeria as a function of temperature, pH and organic acid concentration. Int. J. Food Microbiol. 73:219-237.
116. Levine, P., B. Rose, S. Green, G. Ransom, and W. Hill. 2001. Pathogen testing of ready-to-eat meat and poultry products collected at federally inspected establishments in the United States, 1990 to 1999. J. Food Prot. 64:1188-1193.
117. Linnan, M. J., L. Mascola, X. D. Lou, V. Goulet, S. May, C. Salminen, D. W. Hird, M. L. Yonekura, P. Hayes, R. Weaver, et al. 1988. Epidemic listeriosis associated with Mexican-style cheese. N. Engl. J. Med. 319:823-828.
118. Loken, T., E. Aspoy, and H. Gronstol. 1982. Listeria monocytogenes excretion and humoral immunity in goats in a herd with outbreaks of listeriosis and in a healthy herd. Acta Vet. Scand. 23:392-399.
119. Low, J. C., R. M. Chalmers, W. Donachie, R. Freeman, J. McLauchlin, and P. R. Sisson. 1992. Pyrolysis mass spectrometry of Listeria monocytogenes isolates from sheep. Res. Vet. Sci. 53:64-67.
120. Low, J. C., and W. Donachie. 1997. A review of Listeria monocytogenes and listeriosis. Vet. J. 153:9-29.
121. Low, J. C., W. Donachie, J. McLauchlin, and F. Wright. 1995. Characterization of Listeria monocytogenes strains from a farm environment. Proceedings of XII international symposium on problems of listeriosis, Perth, Western Australia, Promaco Conventions Pty, pp. 141-144.
122. Low, J. C., and C. P. Renton. 1985. Septicaemia, encephalitis and abortions in a housed flock of sheep caused by Listeria monocytogenes type 1/2. Vet. Rec. 116:147-150.
123. Lozniewski, A., A. Humbert, D. Corsaro, J. Schwartzbrod, M. Weber, and A. Le Faou. 2001. Comparison of sludge and clinical isolates of Listeria monocytogenes. Lett. Appl. Microbiol. 32:336-339.
124. Ly, T. M., and H. E. Muller. 1989. Heat tolerance of free living and of intracellular Listeria. Zentralbl. Hyg. Umweltmed. 189:272-276.
125. Ly, T. M., and H. E. Muller. 1990. Ingested Listeria monocytogenes survive and multiply in protozoa. J. Med. Microbiol. 33:51-54.
126. Lyytikainen, O., T. Autio, R. Maijala, P. Ruutu, T. Honkanen-Buzalski, M. Miettinen, M. Hatakka, J. Mikkola, V. J. Anttila, T. Johansson, L. Rantala, T. Aalto, H. Korkeala, and A. Siitonen. 2000. An outbreak of Listeria monocytogenes serotype 3a infections from butter in Finland. J. Infect. Dis. 181:1838-1841.
127. MacDonald, F., and A. D. Sutherland. 1994. Important differences between the generation times of Listeria monocytogenes and Listeria innocua in two Listeria enrichment broths. J. Dairy Res. 61:433-436.
128. MacGowan, A. P., K. Bowker, J. McLauchlin, P. M. Bennett, and D. S. Reeves. 1994. The occurrence and seasonal changes in the isolation of Listeria spp. in shop bought food stuffs, human feces, sewage and soil from urban sources. Int. J. Food. Microbiol. 21:325-334.
129. Mainou-Fowler, T., A. P. MacGowan, and R. Postlethwaite. 1988. Virulence of Listeria spp.: Course of infection in resistant and susceptible mice. J. Med. Microbiol. 27:131-140.
130. McDonald, P., A. R. Henderson, and S. J. E. Heron. 1991. The biochemistry of silage. Marlow, England: Chalcombe Publications.
131. McLauchlin, J. 1996. The relationship between Listeria and listeriosis. Food Control. 7.
132. McLauchlin, J., and G. S. Nichols. 1994. Listeria and seafood. PHLS Microbiol. Dig. 11:151-154.
133. Miettinen, M. K., L. Palmu, K. J. Bjorkroth, and H. Korkeala. 2001. Prevalence of Listeria monocytogenes in broilers at the abattoir, processing plant, and retail level. J. Food Prot. 64:994-999.
134. Milohanic, E., P. Glaser, J. Y. Coppee, L. Frangeul, Y. Vega, J. A. Vazquez-Boland, F. Kunst, P. Cossart, and C. Buchrieser. 2003. Transcriptome analysis of Listeria monocytogenes identifies three groups of genes differently regulated by PrfA. Mol. Microbiol. 47:1613-1625.
135. Mitscherlich, E., and E. H. Marth. 1984. Microbial Survival in the Environment-Bacteria and Rickettsiae Important in Human and Animal Health. Berlin: Springer-Verlag.
136. Motes, M. L. 1991. Incidence of Listeria species in shrimp, oysters, and estuarine waters. J. Food Prot. 54.
137. Nadon, C. A., D. L. Woodward, C. Young, F. G. Rodgers, and M. Wiedmann. 2001. Correlations between molecular subtyping and serotyping of Listeria monocytogenes. J. Clin. Microbiol. 39:2704-2707.
138. Nagi, M. S., and J. D. Verma. 1967. An outbreak of listeriosis in chickens. Ind. J. Vet. Med. 44:539-543.
139. Nesbakken, T., G. Kapperud, and D. A. Caugant. 1996. Pathways of Listeria monocytogenes contamination in the meat processing industry. Int. J. Food Microbiol. 31:161-171.
140. Nicolas, J. A., E. P. Espaze, J. Rocourt, M. J. Cornuejols, M. Lamachere, N. Vidaued, B. Catimel, and A. L. Courtieu. 1988. Listeriose animale et ensilage. Rec. de Med. Vet. 164:203-206.
141. Nilsson, A., and K. A. Karlsson. 1959. Listeria monocytogenes isolations from animals in Sweden during 1948 to 1957. Nord. Vet. Med. 11:305-315.
142. Nolan, D. A., D. C. Chamblin, and J. A. Troller. 1992. Minimal water activity levels for growth and survival of Listeria monocytogenes and Listeria innocua. Int. J. Food Microbiol. 16:323-335.
143. Norrung, B., and J. K. Andersen. 2000. Variations in virulence between different electrophoretic types of Listeria monocytogenes. Lett. Appl. Microbiol. 30:228-232.
144. Norrung, B., and N. Skovgaard. 1993. Application of multilocus enzyme electrophoresis in studies of the epidemiology of Listeria monocytogenes in Denmark. Appl. Environ. Microbiol. 59:2817-2822.
145. Norton, D. M., M. A. McCamey, K. L. Gall, J. M. Scarlett, K. J. Boor, and M. Wiedmann. 2001. Molecular studies on the ecology of Listeria monocytogenes in the smoked fish processing industry. Appl. Environ. Microbiol. 67:198-205.
146. Norton, D. M., J. M. Scarlett, K. Horton, D. Sue, J. Thimothe, K. J. Boor, and M. Wiedmann. 2001. Characterization and pathogenic potential of Listeria monocytogenes isolates from the smoked fish industry. Appl. Environ. Microbiol. 67:646-653.
147. Olafson, P. 1940. Listerella encephalitis (circling disease) of sheep, cattle, and goats. Cornell Vet. 30:141-150.
148. Park, S. F., and R. G. Kroll. 1993. Expression of listeriolysin and phosphatidylinositol-specific phospholipase C is repressed by the plant-derived molecule cellobiose in Listeria monocytogenes. Mol. Microbiol. 8:653-661.
149. Phan-Thanh, L., and T. Gormon. 1995. Analysis of heat and cold shock proteins in Listeria by twodimensional electrophoresis. Electrophoresis 16:444-450.
150. Piffaretti, J. C., H. Kressebuch, M. Aeschbacher, J. Bille, E. Bannerman, J. M. Musser, R. K. Selander, and J. Rocourt. 1989. Genetic characterization of clones of the bacterium Listeria monocytogenes causing epidemic disease. Proc. Natl. Acad. Sci. USA 86:3818-3822.
151. Pinto, B., and D. Reali. 1996. Prevalence of Listeria monocytogenes and other Listeria in Italianmade soft cheeses. Zentralbl. Hyg. Umweltmed. 199:60-68.
152. Plagemann, O., and A. Weber. 1988. Listeria monocytogenes als Abortursache bei Klippschliefern (Procavia capensis). Kleintierpraxis 33:317-318.
153. Pritchard, T. J., K. J. Flanders, and C. W. Donnelly. 1995. Comparison of the incidence of Listeria on equipment versus environmental sites within dairy processing plants. Int. J. Food Microbiol. 26:375-384.
154. Rahman, T., D. K. Sarma, B. K. Goswami, T. N. Upadhyaya, and B. Choudhury. 1985. Occurrence of listerial meningoencephalitis in pigs. Ind. Vet. J. 62:7-9.
155. Ralovich, B. 1984. Listeriosis research-present situation and perspective. Budapest: Akademiai Kiado.
156. Ramage, C. P., J. C. Low, J. McLauchlin, and W. Donachie. 1999. Characterization of Listeria ivanovii isolates from the U.K. using pulsed-field gel electrophoresis. FEMS Microbiol. Lett. 170:349-353.
157. Ranjard, L., F. Poly, and S. Nazaret. 2000. Monitoringcomplex bacterialcommunities using cultureindependent molecular techniques: Application to soil environment. Res. Microbiol. 151:167-177.
158. Razavilar, V., and C. Genigeorgis. 1998. Prediction of Listeria spp. growth as affected by various levels of chemicals, pH, temperature and storage time in a model broth. Int. J. Food Microbiol. 40:149-157.
159. Restaino, L., E. W. Frampton, R. M. Irbe, G. Schabert, and H. Spitz. 1999. Isolation and detection of Listeria monocytogenes using fluorogenic and chromogenic substrates for phosphatidylinositolspecific phospholipase C. J. Food Prot. 62:244-251.
160. Richmond, M. 1990. The microbiological safety of foods, parts I & II. Report of the Committee on the Microbiological Safety of Foods. London: HMSO.
161. Rørvik, L. M., B. Aase, T. Alvestad, and D. A. Caugant. 2003. Molecular epidemiological survey of Listeria monocytogenes in broilers and poultry products. J. Appl. Microbiol. 94:633-640.
162. Rørvik, L. M., D. A. Caugant, and M. Yndestad. 1995. Contamination pattern of Listeria monocytogenes and other Listeria spp. in a salmon slaughterhouse and smoked salmon processing plant. Int. J. Food Microbiol. 25:19-27.
163. Ryser, E. T., S. M. Arimi, M. M. Bunduki, and C. W. Donnelly. 1996. Recovery of different Listeria ribotypes from naturally contaminated, raw refrigerated meat and poultry products with two primary enrichment media. Appl. Environ. Microbiol. 62:1781-1787.
164. Ryser, E. T., S. M. Arimi, and C. W. Donnelly. 1997. Effects of pH on distribution of Listeria ribotypes in corn, hay, and grass silage. Appl. Environ. Microbiol. 63:3695-3697.
165. Sabanadesan, S., A. M. Lammerding, and M. W. Griffiths. 2000. Survival of Listeria innocua in salmon following cold-smoke application. J. Food Prot. 63:715-720.
166. Salamina, G., E. Dalle Donne, A. Niccolini, G. Poda, D. Cesaroni, M. Bucci, R. Fini, M. Maldini, A. Schuchat, B. Swaminathan, W. Bibb, J. Rocourt, N. Binkin, and S. Salmaso. 1996. A foodborne outbreak of gastroenteritis involving Listeria monocytogenes. Epidemiol. Infect. 117:429-436.
167. Salcedo, C., L. Arreaza, B. Alcala, L. De La Fuente, and J. A. Vazquez. 2003. Development of a multilocus sequence typing method for analysis of Listeria monocytogenes clones. J. Clin. Microbiol. 41:757-762.
168. Sauders, B.D. 2003. Unpublished data.
169. Sauders, B. D., E. D. Fortes, D. L. Morse, N. Dumas, J. A. Kiehlbauch, Y. Schukken, J. R. Hibbs, and M. Wiedmann. 2003. Molecular subtyping to detect human listeriosis clusters. Emerg. Infect. Dis. 9:672-680.
170. Schlech, W. F., III, P. M. Lavigne, R. A. Bortolussi, A. C. Allen, E. V. Haldane, A. J. Wort, A. W. Hightower, S. E. Johnson, S. H. King, E. S. Nicholls, and C. V. Broome. 1983. Epidemic listeriosis- evidence for transmission by food. N. Engl. J. Med. 308:203-206.
171. Schneegurt, M. A., S. Y. Dore, and C. F. Kulpa, Jr. 2003. Direct extraction of DNA from soils for studies in microbial ecology. Curr. Issues Mol. Biol. 5:1-8.
172. Schuchat, A., C. Lizano, C. V. Broome, B. Swaminathan, C. Kim, and K. Winn. 1991. Outbreak of neonatal listeriosis associated with mineral oil. Pediatr. Infect. Dis. J. 10:183-189.
173. Schwartz, J. C. 1969. Attempted isolations of Listeria monocytogenes from diagnostic accessions. Am. J. Vet. Res. 30:483-484.
174. Seeliger, H. P. R. 1961. Listeriosis. New York: Hafner Publishing.
175. Senczek, D., R. Stephan, and F. Untermann. 2000. Pulsed-field gel electrophoresis (PFGE) typing of Listeria strains isolated from a meat processing plant over a 2-year period. Int. J. Food Microbiol. 62:155-159.
176. Skjerve, E., L. M. Rørvik, and O. Olsvik. 1990. Detection of Listeria monocytogenes in foods by immunomagnetic separation. Appl. Environ. Microbiol. 56:3478-3481.
177. Skovgaard, N., and C. A. Morgen. 1988. Detection of Listeria spp. in feces from animals, in feeds, and in raw foods of animal origin. Int. J. Food Microbiol. 6:229-242.
178. Sontakke, S., and J. M. Farber. 1995. The use of PCR ribotyping for typing strains of Listeria spp. Eur. J. Epidemiol. 11:665-673.
179. Soontharanont, S., and C. D. Garland. 1995. The occurrence of Listeria in temperate aquatic habitats. Proceedings of XII international symposium on problems of listeriosis, Perth, Western Australia, Promaco Conventions, pp. 145-146.
180. Sturgess, C. P. 1989. Listerial abortion in the bitch. Vet. Rec. 124:177.
181. Takai, S., F. Orii, K. Yasuda, S. Inoue, and S. Tsubaki. 1990. Isolation of Listeria monocytogenes from raw milk and its environment at dairy farms in Japan. Microbiol. Immunol. 34:631-634.
182. Thimothe, J., J. Walker, V. Suvanich, K. L. Gall, M. W. Moody, and M. Wiedmann. 2002. Detection of Listeria in crawfish processing plants and in raw, whole crawfish and processed crawfish (Procambarus spp.). J. Food Prot. 65:1735-1739.
183. Tompkin, R. B. 2002. Control of Listeria monocytogenes in the food-processing environment. J. Food Prot. 65:709-725.
184. Trott, D. J., I. D. Robertson, and D. J. Hampson. 1993. Genetic characterization of isolates of Listeria monocytogenes from man, animals and food. J. Med. Microbiol. 38:122-128.
185. Troxler, R., A. von Graevenitz, G. Funke, B. Wiedemann, and I. Stock. 2000. Natural antibiotic susceptibility of Listeria species: L. grayi, L. innocua, L. ivanovii, L. monocytogenes, L. seeligeri and L. welshimeri strains. Clin. Microbiol. Infect. 6:525-535.
186. Ueno, H., K. Yokota, T. Arai, Y. Muramatsu, H. Taniyama, T. Iida, and C. Morita. 1996. The prevalence of Listeria monocytogenes in the environment of dairy farms. Microbiol. Immunol. 40:121-124.
187. Uyttendaele, M., P. De Troy, and J. Debevere. 1999. Incidence of Salmonella, Campylobacter jejuni, Campylobacter coli, and Listeria monocytogenes in poultry carcasses and different types of poultry products for sale on the Belgian retail market. J. Food Prot. 62:735-740.
188. van Netten, P., I. Perales, A. van de Moosdijk, G. D. Curtis, and D. A. Mossel. 1989. Liquid and solid selective differential media for the detection and enumeration of L. monocytogenes and other Listeria spp. Int. J. Food Microbiol. 8:299-316.
189. Van Renterghem, B., F. Huysman, R. Rygole, and W. Verstraete. 1991. Detection and prevalence of Listeria monocytogenes in the agricultural ecosystem. J. Appl. Bacteriol. 71:211-217.
190. Vazquez-Boland, J. A., L. Dominguez, M. Blanco, J. Rocourt, J. F. Fernandez-Garayzabal, C. B. Gutierrez, R. I. Tascon, and E. F. Rodriguez-Ferri. 1992. Epidemiologic investigation of a silageassociated epizootic of ovine listeric encephalitis, using a new Listeria-selective enumeration medium and phage typing. Am. J. Vet. Res. 53:368-371.
191. Vazquez-Boland, J. A., M. Kuhn, P. Berche, T. Chakraborty, G. Dominguez-Bernal, W. Goebel, B. Gonzalez-Zorn, J. Wehland, and J. Kreft. 2001. Listeria pathogenesis and molecular virulence determinants. Clin. Microbiol. Rev. 14:584-640.
192. Vela, A. I., J. F. Fernandez-Garayzabal, J. A. Vazquez, M. V. Latre, M. M. Blanco, M. A. Moreno, L. de La Fuente, J. Marco, C. Franco, A. Cepeda, A. A. Rodriguez Moure, G. Suarez, and L. Dominguez. 2001. Molecular typing by pulsed-field gel electrophoresis of Spanish animal and human Listeria monocytogenes isolates. Appl. Environ. Microbiol. 67:5840-5843.
193. Vizcaino, L. L., M. J. Cubero, and A. Contreras. 1988. Listeric abortions in ewes and cows associated to orange peel and artichoke silage feeding. Proceedings of X international symposium on listeriosis, Pecs, Hungary, p. P29.
194. Vlaemynck, G., L. Herman, and K. Coudijzer. 1994. Isolation and characterization of two bacteriocins produced by Enterococcus faecium strains inhibitory to Listeria monocytogenes. Int. J. Food Microbiol. 24:211-225.
195. Volokhov, D., A. Rasooly, K. Chumakov, and V. Chizhikov. 2002. Identification of Listeria species by microarray-based assay. J. Clin. Microbiol. 40:4720-4728.
196. Walker, J. K., and J. H. Morgan. 1993. Ovine ophthalmitis associated with Listeria monocytogenes. Vet. Rec. 132:636.
197. Watkins, J., and K. P. Sleath. 1981. Isolation and enumeration of Listeria monocytogenes from sewage, sewage sludge and river water. J. Appl. Bacteriol. 50:1-9.
198. Weis, J., and H. P. Seeliger. 1975. Incidence of Listeria monocytogenes in nature. Appl. Microbiol. 30:29-32.
199. Welsh, R. D. 1983. Equine abortion caused by Listeria monocytogenes serotype 4. J. Am. Vet. Med. Assoc. 182:291.
200. Welshimer, H. J. 1960. Survival of Listeria monocytogenes in soil. J. Bacteriol. 80:316-320.
201. Welshimer, H. J., and J. Donker-Voet. 1971. Listeria monocytogenes in nature. Appl. Microbiol. 21:516-519.
202. Wesley, I. V., and F. Ashton. 1991. Restriction enzyme analysis of Listeria monocytogenes strains associated with food-borne epidemics. Appl. Environ. Microbiol. 57:969-975.
203. Whittenbury, R. 1968. Microbiology of silage grass. Proces. Biochem. 3:27-31.
204. Wiedmann, M. 2002. Molecular subtyping methods for Listeria monocytogenes. J. AOAC Int. 85:524-531.
205. Wiedmann, M., T. Arvik, J. L. Bruce, J. Neubauer, F. del Piero, M. C. Smith, J. Hurley, H. O. Mohammed, and C. A. Batt. 1997. Investigation of a listeriosis epizootic in sheep in New York state. Am. J. Vet. Res. 58:733-737.
206. Wiedmann, M., J. L. Bruce, C. Keating, A. E. Johnson, P. L. McDonough, and C. A. Batt. 1997. Ribotypes and virulence gene polymorphisms suggest three distinct Listeria monocytogenes lineages with differences in pathogenic potential. Infect. Immun. 65:2707-2716.
207. Wiedmann, M., J. L. Bruce, R. Knorr, M. Bodis, E. M. Cole, C. I. McDowell, P. L. McDonough, and C. A. Batt. 1996. Ribotype diversity of Listeria monocytogenes strains associated with outbreaks of listeriosis in ruminants. J. Clin. Microbiol. 34:1086-1090.
208. Wiedmann, M., J. Czajka, N. Bsat, M. Bodis, M. C. Smith, T. J. Divers, and C. A. Batt. 1994. Diagnosis and epidemiological association of Listeria monocytogenes strains in two outbreaks of listerial encephalitis in small ruminants. J. Clin. Microbiol. 32:991-996.
209. Wiedmann, M., and K. E. Evans. 2000. Infectious diseases of dairy animals: Listeriosis. In Encyclopedia of dairy science. London: Academic Press.
210. Wiedmann, M., S. Mobini, J. R. Cole, Jr., C. K. Watson, G. T. Jeffers, and K. J. Boor. 1999. Molecular investigation of a listeriosis outbreak in goats caused by an unusual strain of Listeria monocytogenes. J. Am. Vet. Med. Assoc. 215:369-371, 340.
211. Wilson, I. G. 1995. Occurrence of Listeria species in ready to eat foods. Epidemiol. Infect. 115:519-526.
212. Winkenwerder, W. 1967. The occurrence of Listeria monocytogenes in cattle in Niedersachsen. Berl. Münch. Tierärztl. Wochenschr. 80:445-449.
213. Yu, L. S., and D. Y. Fung. 1993. Five-tube most-probable-number method using the Fung-Yu tube for enumeration of Listeria monocytogenes in restructured meat products during refrigerated storage. Int. J. Food Microbiol. 18:97-106.