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This report described the common contaminants in reagents and glassware, such as oleic, palmitic, and stearic acids, that interfere with results in lipidomics, and approaches to reduce abundant fatty acid contamination to a level usually below the amount that can be detected in the sample of interest.
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Bollinger, J.G., Naika, G.S., Sadilek, M., Gelb, M.H., LC/ESI-MS/MS detection of FAs by charge reversal derivatization with more than four orders of magnitude improvement in sensitivity. J Lipid Res 54 (2013), 3523–3530 This report described the common contaminants in reagents and glassware, such as oleic, palmitic, and stearic acids, that interfere with results in lipidomics, and approaches to reduce abundant fatty acid contamination to a level usually below the amount that can be detected in the sample of interest.
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This review covered chemical derivatization strategies to comprehensively identify and structurally characterize lipid species with in complex mixtures.
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Ryan, E., Reid, G.E., Chemical derivatization and ultrahigh resolution and accurate mass spectrometry strategies for “shotgun” lipidome analysis. Acc Chem Res 49 (2016), 1596–1604 This review covered chemical derivatization strategies to comprehensively identify and structurally characterize lipid species with in complex mixtures.
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This work introduced acharge-switch derivatization of fatty acids method toidentification and quantitation of the specific locations of double bonds.
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Yang, K., Dilthey, B.G., Gross, R.W., Identification and quantitation of fatty acid double bond positional isomers: a shotgun lipidomics approach using charge-switch derivatization. Anal Chem 85 (2013), 9742–9750 This work introduced acharge-switch derivatization of fatty acids method toidentification and quantitation of the specific locations of double bonds.
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An one-step derivatization method for characterization and quantification of diacylglycerol species by shotgun lipidomics was reporte.
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Wang, M., Hayakawa, J., Yang, K., Han, X., Characterization and quantification of diacylglycerol species in biological extracts after one-step derivatization: a shotgun lipidomics approach. Anal Chem 86 (2014), 2146–2155 An one-step derivatization method for characterization and quantification of diacylglycerol species by shotgun lipidomics was reporte.
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This review focused on advances of shotgun lipidomics and its applications in biological research.
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Wang, M., Wang, C., Han, R.H., Han, X., Novel advances in shotgun lipidomics for biology and medicine. Prog Lipid Res 61 (2016), 83–108 This review focused on advances of shotgun lipidomics and its applications in biological research.
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Acomprehensive strategy based on the pseudotargeted analysis by integrating blank-wash, pooled quality control (QC) sample was developed to provide a reliable protocol for large-scale metabolomics studies. It is also very important in lipidomics research.
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Luo, P., Yin, P., Zhang, W., Zhou, L., Lu, X., Lin, X., Xu, G., Optimization of large-scale pseudotargeted metabolomics method based on liquid chromatography-mass spectrometry. J Chromatogr A 1437 (2016), 127–136 Acomprehensive strategy based on the pseudotargeted analysis by integrating blank-wash, pooled quality control (QC) sample was developed to provide a reliable protocol for large-scale metabolomics studies. It is also very important in lipidomics research.
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This paper presented the publicly availableLipidBlast software for the development of MS/MS libraries for lipid classes.
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Kind, T., Okazaki, Y., Saito, K., Fiehn, O., LipidBlast templates as flexible tools for creating new in-silico tandem mass spectral libraries. Anal Chem 86 (2014), 11024–11027 This paper presented the publicly availableLipidBlast software for the development of MS/MS libraries for lipid classes.
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