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Volumn 7, Issue 3, 2004, Pages 337-344

Lipid species profiling: A high-throughput approach to identify lipid compositional changes and determine the function of genes involved in lipid metabolism and signaling

Author keywords

DGDG; digalactosyldiacylglycerol; electrospray ionization; ESI; mass spectrometry; MGDG; monogalactosyldiacylglycerol; MS; PA; PC; PE; PG; phosphatidic acid; phosphatidylcholine; phosphatidylethanolamine; phosphatidylglycerol; phosphatidylinositol; PI; PLD

Indexed keywords

LIPID;

EID: 2442617114     PISSN: 13695266     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pbi.2004.03.011     Document Type: Review
Times cited : (165)

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    • Deuterium labeling of yeast with two different precursors, followed by ESI-MS/MS analysis of the molecular species of PE and PC, demonstrated that PC is produced both by PE triple methylation and from choline and diacylglycerol via cytosine diphosphate (CDP)-choline. The two pathways produce different PC molecular species. The combination of lipid profiling and stable isotope labeling should prove useful in elucidating other metabolic pathways.
    • Boumann H.A., Damen M.J.A., Versluis C., Heck A.J.R., de Kruijff B., de Kroon A.I.P.M. The two biosynthetic routes leading to phosphatidylcholine in yeast produce different sets of molecular species. Evidence for lipid remodeling. Biochemistry. 42:2003;3054-3059 Deuterium labeling of yeast with two different precursors, followed by ESI-MS/MS analysis of the molecular species of PE and PC, demonstrated that PC is produced both by PE triple methylation and from choline and diacylglycerol via cytosine diphosphate (CDP)-choline. The two pathways produce different PC molecular species. The combination of lipid profiling and stable isotope labeling should prove useful in elucidating other metabolic pathways.
    • (2003) Biochemistry , vol.42 , pp. 3054-3059
    • Boumann, H.A.1    Damen, M.J.A.2    Versluis, C.3    Heck, A.J.R.4    De Kruijff, B.5    De Kroon, A.I.P.M.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.