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Mutants of Arabidopsis reveal many roles for membrane lipids
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This excellent review summarizes current knowledge of lipid metabolism and identifies areas that are ripe for further investigation.
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Wallis J.G., Browse J. Mutants of Arabidopsis reveal many roles for membrane lipids. Prog Lipid Res. 41:2002;254-278 This excellent review summarizes current knowledge of lipid metabolism and identifies areas that are ripe for further investigation.
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A novel technique for the sensitive quantification of acyl CoA esters from plant tissues
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Quantification of jasmonic acid, methyl jasmonate, and salicylic acid in plants by capillary liquid chromatography electrospray ionization tandem mass spectrometry
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Wilbert S.M., Ericsson L.H., Gordon M.P. Quantification of jasmonic acid, methyl jasmonate, and salicylic acid in plants by capillary liquid chromatography electrospray ionization tandem mass spectrometry. Anal Biochem. 257:1998;186-194
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Thoma I., Loeffler C., Sinha A.K., Gupta M., Kischeke M., Steffan B., Roitsch T., Mueller M.J. Cyclopentenone isoprostanes induced by reactive oxygen species trigger defense gene activation and phytolalexin accumulation in plants. Plant J. 34:2003;363-375
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Analysis of oxylipins by high-performance liquid chromatography with evaporative light-scattering detection and particle-beam mass spectrometry
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Hamberg M., Sanz A., Castresana C. α-oxidation of fatty acids in higher plants: identification of a pathogen-inducible oxygenase (piox) as an α-dioxygenase and biosynthesis of 2-hydroperoxylinolenic acid. J Biol Chem. 274:1999;24503-24513
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Kramell R., Miersch O., Atzorn R., Parthier B., Wasternack C. Octadecanoid-derived alteration of gene expression and the "oxylipin signature" in stressed barley leaves. Implications for different signaling pathways. Plant Physiol. 123:2000;177-187
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Oxylipin profiling reveals the preferential stimulation of the 9-lipoxygenase pathway in elicitor-treated potato cells
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Göbel C., Feussner I., Schmidt A., Scheel D., Sanchez-Serrano J., Hamberg M., Rosahl S. Oxylipin profiling reveals the preferential stimulation of the 9-lipoxygenase pathway in elicitor-treated potato cells. J Biol Chem. 276:2001;6267-6273
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18
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A permease-like protein involved in ER to thylakoid lipid transfer in Arabidopsis
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A robotic procedure for lipid extraction and thin-layer chromatography was applied to screen 25 000 seedlings for the production of altered levels of digalactosydiacylglycerol and the accumulation of an extra class of lipid, trigalactosyldiacylglycerol. Analysis of one of the mutants that accumulated trigalactosyldiacylglycerol demonstrated that this mutant was defective in a permease-like protein of the outer chloroplastic envelope, which is proposed to be part of a lipid-transfer complex.
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Xu C., Fan J., Riekhof W., Froehlich J.E., Benning C. A permease-like protein involved in ER to thylakoid lipid transfer in Arabidopsis. EMBO J. 22:2003;2370-2379 A robotic procedure for lipid extraction and thin-layer chromatography was applied to screen 25 000 seedlings for the production of altered levels of digalactosydiacylglycerol and the accumulation of an extra class of lipid, trigalactosyldiacylglycerol. Analysis of one of the mutants that accumulated trigalactosyldiacylglycerol demonstrated that this mutant was defective in a permease-like protein of the outer chloroplastic envelope, which is proposed to be part of a lipid-transfer complex.
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Xu, C.1
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19
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Separation of phospholipids in microfluid chip device: Application to high-throughput screening assays for lipid-modifying enzymes
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Microfluid-chip-based technology was used to separate lipid substrates and products after enzymatic reactions using fluorescently labeled substrates and products. This technology can be utilized for high-throughput screening for the presence of lipid-modifying enzymes.
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Lin S., Fischl A.S., Bi X., Parce W. Separation of phospholipids in microfluid chip device: application to high-throughput screening assays for lipid-modifying enzymes. Anal Biochem. 314:2003;97-107 Microfluid-chip-based technology was used to separate lipid substrates and products after enzymatic reactions using fluorescently labeled substrates and products. This technology can be utilized for high-throughput screening for the presence of lipid-modifying enzymes.
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Lin, S.1
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The sulfolipids 2′-O-acyl-sulfoquinovosyldiacylglycerol and sulfoquinovosyldiacylglycerol are absent from a Chlamydomonas reinhardtii mutant deleted in SQD11
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Riekhof W.R., Ruckle M.E., Lydic T.A., Sears B.B., Benning C. The sulfolipids 2′-O-acyl-sulfoquinovosyldiacylglycerol and sulfoquinovosyldiacylglycerol are absent from a Chlamydomonas reinhardtii mutant deleted in SQD11. Plant Physiol. 133:2003;864-874
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Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry
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Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI/mass spectrometry: A bridge to lipidomics
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More than ten years of work on the development of lipid profiling are boiled down to a succinct description of the authors' working methods and to highlights of their recent work on the roles of lipids in human and animal disease processes.
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Han X., Gross R.W. Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI/mass spectrometry: a bridge to lipidomics. J Lipid Res. 44:2003;1071-1079 More than ten years of work on the development of lipid profiling are boiled down to a succinct description of the authors' working methods and to highlights of their recent work on the roles of lipids in human and animal disease processes.
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Han, X.1
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Han X., Gross R.W. Electrospray ionization mass spectroscopic analysis of human erythrocyte plasma membrane phospholipids. Proc Natl Acad Sci USA. 91:1994;10635-10639
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Alterations of individual molecular species of human platelet phospholipids during thrombin stimulation: Electrospray-ionization mass spectrometry-facilitated identification of the boundary conditions for the magnitude and selectivity of thrombin-induced platelet phospholipid hydrolysis
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Han X., Gubitosi-Klug R.A., Collins B.J., Gross R.W. Alterations of individual molecular species of human platelet phospholipids during thrombin stimulation: electrospray-ionization mass spectrometry-facilitated identification of the boundary conditions for the magnitude and selectivity of thrombin-induced platelet phospholipid hydrolysis. Biochemistry. 35:1996;5822-5832
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Electrospray ionization mass spectrometric analyses of phospholipids from rat and human pancreatic islets and subcellular membranes: Comparison to other tissues and implication for membrane fusion in insulin exocytosis
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Ramanadham S., Hsu F.-F., Bohrer A., Nowatzke W., Ma Z., Turk J. Electrospray ionization mass spectrometric analyses of phospholipids from rat and human pancreatic islets and subcellular membranes: comparison to other tissues and implication for membrane fusion in insulin exocytosis. Biochemistry. 37:1998;4553-4567
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Structural characterization of triacylglycerols as lithiated adducts by electrospray ionization mass spectrometry using low-energy collisionally activated dissociation on a triple-stage quadrupole instrument
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Hsu F.-F., Turk J. Structural characterization of triacylglycerols as lithiated adducts by electrospray ionization mass spectrometry using low-energy collisionally activated dissociation on a triple-stage quadrupole instrument. J Am Soc Mass Spectrom. 10:1999;587-599
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Studies of the role of group VI phospholipase A2 in fatty acid incorporation, phospholipid remodeling, lysophosphatidylcholine generation, and secretagogue-induced arachidonic acid release in pancreatic islets and insulinoma cells
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Ramanadham S., Hsu F.-F., Bohrer A., Ma Z., Turk J. Studies of the role of group VI phospholipase A2 in fatty acid incorporation, phospholipid remodeling, lysophosphatidylcholine generation, and secretagogue-induced arachidonic acid release in pancreatic islets and insulinoma cells. J Biol Chem. 274:1999;13915-13927
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Hsu F.-F., Turk J. Characterization of phosphatidylinositol, phosphatidylinositol-4-phosphate, and phosphatidylinositol-4, 5-bisphosphate by electrospray ionization tandem mass spectrometry: a mechanistic study. J Am Soc Mass Spectrom. 11:2000;986-999
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Ramanadham S., Hsu F., Zhang S., Bohrer A., Ma Z., Turk J. Electrospray ionization mass spectrometric analyses of phospholipids from INS-1 insulinoma cells: comparison to pancreatic islets and effects of fatty acid supplementation on phospholipid composition and insulin secretion. Biochim Biophys Acta. 1484:2000;251-266
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Quantitative analysis and molecular species fingerprinting of triacylglyceride molecular species directly from lipid extracts of biological samples by electrospray ionization tandem mass spectrometry
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Han X., Gross R.W. Quantitative analysis and molecular species fingerprinting of triacylglyceride molecular species directly from lipid extracts of biological samples by electrospray ionization tandem mass spectrometry. Anal Biochem. 295:2001;88-100
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Listenberger L.L., Han X., Lewis S.E., Cases S., Farese R.V. Jr., Ory D.S., Schaffer J.E. Triglyceride accumulation protects against fatty acid-induced lipotoxicity. Proc Natl Acad Sci USA. 100:2003;3077-3082
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Listenberger, L.L.1
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0347065337
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Decreased hepatic triglyceride accumulation and altered fatty acid uptake in mice with deletion of the liver fatty acid-binding protein gene
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Newberry E.P., Xie Y., Kennedy S., Han X., Buhman K.K., Luo J., Gross R.W., Davidson N.O. Decreased hepatic triglyceride accumulation and altered fatty acid uptake in mice with deletion of the liver fatty acid-binding protein gene. J Biol Chem. 278:2003;51664-51672
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Newberry, E.P.1
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Gross, R.W.7
Davidson, N.O.8
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35
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0037199955
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Profiling membrane lipids in plant stress responses: Role of phospholipase Dα in freezing-induced lipid changes in Arabidopsis
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The authors describe a methodology for the automated profiling of the major plant polar lipid classes. They apply this strategy to identify the changes that occur in Arabidopsis during cold acclimation and freezing. Comparison of wildtype plants and plants that are defective in PLDα define the role of PLDα in the plant response to freezing.
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Welti R., Li W., Li M., Sang Y., Biesiada H., Zhou H.-E., Rajashekar C.B., Williams T.D., Wang X. Profiling membrane lipids in plant stress responses: role of phospholipase Dα in freezing-induced lipid changes in Arabidopsis. J Biol Chem. 277:2002;31994-32002 The authors describe a methodology for the automated profiling of the major plant polar lipid classes. They apply this strategy to identify the changes that occur in Arabidopsis during cold acclimation and freezing. Comparison of wildtype plants and plants that are defective in PLDα define the role of PLDα in the plant response to freezing.
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J Biol Chem
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Welti, R.1
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Zhou, H.-E.6
Rajashekar, C.B.7
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Wang, X.9
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36
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0037334429
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Electrospray ionization tandem mass spectrometry scan modes for plant chloroplast lipids
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The authors describe ESI-MS/MS methods for profiling MGDG, DGDG, SQDG, and PG.
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Welti R., Wang X., Williams T.D. Electrospray ionization tandem mass spectrometry scan modes for plant chloroplast lipids. Anal Biochem. 314:2003;149-152 The authors describe ESI-MS/MS methods for profiling MGDG, DGDG, SQDG, and PG.
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Welti, R.1
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Structural identification of glycerolipid molecular species isolated from Cyanobacterium Synechocystis sp. PCC 6803 using fast atom bombardment tandem mass spectrometry
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Kim Y.H., Choi J.-S., Yoo J.S., Park Y.-M., Kim M.S. Structural identification of glycerolipid molecular species isolated from Cyanobacterium Synechocystis sp. PCC 6803 using fast atom bombardment tandem mass spectrometry. Anal Biochem. 267:1999;260-270
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Kim, Y.H.1
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38
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0038148603
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Lipid and oxylipin profiles during aging and sprout development in potato tubers (Solanum tuberosum L.)
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Decreases in phospholipid levels that occur when stored potato tubers sprout correlate temporally with increases in the levels of the 9-S-hydroperoxide of linoleic acid and of colneleic acid.
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Fauconnier M.L., Welti R., Blée E., Marlier M. Lipid and oxylipin profiles during aging and sprout development in potato tubers (Solanum tuberosum L.). Biochim Biophys Acta. 1633:2003;118-126 Decreases in phospholipid levels that occur when stored potato tubers sprout correlate temporally with increases in the levels of the 9-S-hydroperoxide of linoleic acid and of colneleic acid.
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Biochim Biophys Acta
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Fauconnier, M.L.1
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39
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0142028805
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The Arabidopsis thaliana sfd mutants affect plastidic lipid composition and suppress dwarfing, cell death and the enhanced disease resistance phenotypes resulting from the deficiency of a fatty acid desaturase
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Lipid profiles from plants with three different mutations that suppress the dwarfed phenotype of the ssi2 mutation showed changes in the composition of plastidic lipids. This is the first study to use lipid profiling to investigate mechanisms associated with defense responses.
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Nandi A., Krothapalli K., Buseman C.M., Li M., Welti R., Enyedi A., Shah J. The Arabidopsis thaliana sfd mutants affect plastidic lipid composition and suppress dwarfing, cell death and the enhanced disease resistance phenotypes resulting from the deficiency of a fatty acid desaturase. Plant Cell. 15:2003;2383-2398 Lipid profiles from plants with three different mutations that suppress the dwarfed phenotype of the ssi2 mutation showed changes in the composition of plastidic lipids. This is the first study to use lipid profiling to investigate mechanisms associated with defense responses.
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Plant Cell
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Nandi, A.1
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40
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1042290708
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The Arabidopsis dihydroxyacetone phosphate reductase gene, SUPPRESSOR of FATTY ACID DESATURASE DEFICIENCY1, is required for glycerolipid metabolism and for the activation of systemic acquired resistance
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Profiling of complex lipids from plants that have a mutation that compromises the acquisition of systemic acquired resistance showed that these plants have altered plastidic glycerolipid composition. A candidate gene approach identified the altered gene as a putative dihydroxyacetone phosphate reductase.
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Nandi A., Welti R., Shah J. The Arabidopsis dihydroxyacetone phosphate reductase gene, SUPPRESSOR OF FATTY ACID DESATURASE DEFICIENCY1, is required for glycerolipid metabolism and for the activation of systemic acquired resistance. Plant Cell. 16:2004;465-477 Profiling of complex lipids from plants that have a mutation that compromises the acquisition of systemic acquired resistance showed that these plants have altered plastidic glycerolipid composition. A candidate gene approach identified the altered gene as a putative dihydroxyacetone phosphate reductase.
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Plant Cell
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Nandi, A.1
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41
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0345363228
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Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of the lipid molecular species composition of yeast subcellular membranes reveals acyl chain-based sorting/remodeling of distinct molecular species en route to the plasma membrane
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Schneiter R., Brügger B., Sandhoff R., Zellnig G., Leber A., Lampl M., Athenstaedt K., Hrastnik C., Eder S., Daum G., et al. Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of the lipid molecular species composition of yeast subcellular membranes reveals acyl chain-based sorting/remodeling of distinct molecular species en route to the plasma membrane. J Cell Biol. 146:1999;741-754
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Schneiter, R.1
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42
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0037065727
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Lipid rafts are enriched in arachidonic acid and plasmenylethanolamine and their composition is independent of caveolin-1 expression: A quantitative electrospray ionization/mass spectrometric analysis
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Lipids from lipid rafts and from whole plasma membranes isolated from a human epidermal carcinoma cell line were quantitatively profiled by ESI-MS. These lipid rafts were enriched in sphingomyelin, cholesterol, and plasmenylethanolamines.
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Pike L.J., Han X., Chung K.-N., Gross R.W. Lipid rafts are enriched in arachidonic acid and plasmenylethanolamine and their composition is independent of caveolin-1 expression: a quantitative electrospray ionization/mass spectrometric analysis. Biochemistry. 41:2002;2075-2088 Lipids from lipid rafts and from whole plasma membranes isolated from a human epidermal carcinoma cell line were quantitatively profiled by ESI-MS. These lipid rafts were enriched in sphingomyelin, cholesterol, and plasmenylethanolamines.
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Biochemistry
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Pike, L.J.1
Han, X.2
Chung, K.-N.3
Gross, R.W.4
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The two biosynthetic routes leading to phosphatidylcholine in yeast produce different sets of molecular species. Evidence for lipid remodeling
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Deuterium labeling of yeast with two different precursors, followed by ESI-MS/MS analysis of the molecular species of PE and PC, demonstrated that PC is produced both by PE triple methylation and from choline and diacylglycerol via cytosine diphosphate (CDP)-choline. The two pathways produce different PC molecular species. The combination of lipid profiling and stable isotope labeling should prove useful in elucidating other metabolic pathways.
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Boumann H.A., Damen M.J.A., Versluis C., Heck A.J.R., de Kruijff B., de Kroon A.I.P.M. The two biosynthetic routes leading to phosphatidylcholine in yeast produce different sets of molecular species. Evidence for lipid remodeling. Biochemistry. 42:2003;3054-3059 Deuterium labeling of yeast with two different precursors, followed by ESI-MS/MS analysis of the molecular species of PE and PC, demonstrated that PC is produced both by PE triple methylation and from choline and diacylglycerol via cytosine diphosphate (CDP)-choline. The two pathways produce different PC molecular species. The combination of lipid profiling and stable isotope labeling should prove useful in elucidating other metabolic pathways.
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(2003)
Biochemistry
, vol.42
, pp. 3054-3059
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Boumann, H.A.1
Damen, M.J.A.2
Versluis, C.3
Heck, A.J.R.4
De Kruijff, B.5
De Kroon, A.I.P.M.6
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