Engineered CHO cells for production of diverse, homogeneous glycoproteins

Nature Biotechnology

Volumn 33, Issue 8, 2015, Pages 842-844

  Yang, Zhang ^a,b   Wang, Shengjun ^a,b   Halim, Adnan ^a,b   Schulz, Morten Alder ^a   Frodin, Morten ^c   Rahman, Shamim H ^a,d   Vester Christensen, Malene B ^a,d   Behrens, Carsten ^d   Kristensen, Claus ^a   Vakhrushev, Sergey Y ^a   Bennett, Eric Paul ^a   Wandall, Hans H ^a   Clausen, Henrik ^a,b  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b Chalmers University of Technology   (Denmark)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

^d NOVO NORDISK A S   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOLOGY; GLYCOPROTEINS; GLYCOSYLATION;

CHINESE HAMSTER OVARY CELLS; DESIGN MATRIX; GLYCOFORMS; GLYCOSYL TRANSFERASE; HUMAN LIKE; N-GLYCOSYLATION; SIALIC ACIDS; THERAPEUTIC POTENTIALS;

CELLS;

GLYCOPROTEIN; GLYCOSYLTRANSFERASE; SIALIC ACID; ZINC FINGER NUCLEASE; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; CELL ENGINEERING; CHO CELL LINE; FEMALE; FUCOSYLATION; GENE CONTROL; GENE DELETION; GENE EXPRESSION; GENE INSERTION; GENETIC SCREENING; GENOME; GLYCOSYLATION; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE STUDY; RNA SEQUENCE; SIALYLATION; ANIMAL; CHEMISTRY; CRICETULUS; GENETICS; HAMSTER; METABOLISM; PROCEDURES; PROTEIN ENGINEERING;

CRICETULUS GRISEUS;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; GLYCOPROTEINS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 84938895840     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.3280     Document Type: Article

References (20)

1. Walsh, G. Nat. Biotechnol. 32, 992-1000 (2014).
2. Walsh, G. Drug Discov. Today 15, 773-780 (2010).
3. Sasaki, H., Ochi, N., Dell, A. & Fukuda, M. Biochemistry 27, 8618-8626 (1988).
4. Sinclair, A.M. & Elliott, S. J. Pharm. Sci. 94, 1626-1635 (2005).
5. Patnaik, S.K. & Stanley, P. Methods Enzymol. 416, 159-182 (2006).
6. Yamane-Ohnuki, N. et al. Biotechnol. Bioeng. 87, 614-622 (2004).
7. Malphettes, L. et al. Biotechnol. Bioeng. 106, 774-783 (2010).
8. Steentoft, C. et al. Glycobiology 24, 663-680 (2014).
9. Xu, X. et al. Nat. Biotechnol. 29, 735-741 (2011).
10. Lewis, N.E. et al. Nat. Biotechnol. 31, 759-765 (2013).
11. North, S.J. et al. J. Biol. Chem. 285, 5759-5775 (2010).
12. Amado, M., Almeida, R., Schwientek, T. & Clausen, H. Biochim. Biophys. Acta 1473, 35-53 (1999).
13. Narimatsu, H. Curr. Opin. Struct. Biol. 16, 567-575 (2006).
14. Fukuda, M.N., Sasaki, H., Lopez, L. & Fukuda, M. Blood 73, 84-89 (1989).
15. Maresca, M., Lin, V.G., Guo, N. & Yang, Y. Genome Res. 23, 539-546 (2013).
16. El Maï, N., Donadio-Andrei, S., Iss, C., Calabro, V. & Ronin, C. Methods Mol. Biol. 988, 19-29 (2013).
17. DeFrees, S. et al. Glycobiology 16, 833-843 (2006).
18. Hamilton, S.R. et al. Science 313, 1441-1443 (2006).
19. Duda, K. et al. Nucleic Acids Res. 42, e84 (2014).
20. Zhang, Y. et al. Nucleic Acids Res. 43, e59 (2015).